• Journal of Microbiology and Biotechnology
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• v.33 no.9
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• pp.1170-1178
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• 2023

Food allergy represents a severe problem for many societies, including sensitive populations, academies, health authorities, and the food industry. Peanut allergy occupies a special place in the food allergy spectrum. To prevent consumption by consumers suffering from a peanut allergy, a rapid and sensitive detection method is essential to identify unintended peanut adulteration in processed foods. In this study, we produced four monoclonal antibodies (MAbs; RO 3A1-12, PB 4C12-10, PB 5F9-23, and PB 6G4-30) specific to thermo-stable and soluble proteins (TSSPs) of peanut and developed an enzyme-linked immunosorbent assay (ELISA) based on the MAbs. Among them, PB 5F9-23 MAb was firmly bound to Ara h 1, and other MAbs strongly reacted to Ara h 3 in the Western blot analysis. An antibody cocktail solution of the MAbs was used to enhance the sensitivity of an indirect ELISA, and the limit of detection of the indirect ELISA based on the antibody cocktail solution was 1 ng/ml and improved compared to the indirect ELISA based on the single MAb (11 ng/ml). The cross-reaction analysis revealed the high specificity of developed MAbs to peanut TSSPs without cross-reaction to other food allergens, including nuts. Subsequently, analyzing processed foods by indirect ELISA, all foods labeled as containing peanuts in the product description were confirmed to be positive. The results indicate that the developed antibodies exhibit high specificity and sensitivity to peanuts and can be used as bio-receptors in immunoassays or biosensors to detect intentional or unintentional adulteration of peanuts in processed foods, particularly heat-processed foods.

• Journal of Environmental Science International
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• v.24 no.6
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• pp.763-767
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• 2015

The present study was conducted to development mass production methods for peanut sprouts that is considered as a field of blue ocean among the agricultural products. 'Jopyeong' was the best as a major cultivar for peanut sprouts production. The manual for the production of high-quality peanut sprouts is as following. Germination temperature appropriate for production of high-quality peanut sprouts was $27^{\circ}C$. Peanut sprouts at the growth stage of 8th day, and older plants with advanced growth showed deteriorated merchantable and eating quality. Resveratrol compound was not found in the seeds, but its highest amount was detected from 9-day old sprouts. The best water temperature applicable to high quality peanut sprout production was $25^{\circ}C$. The growth of peanut sprout was inhibited by the high temperatures above $35^{\circ}C$ and low temperatures below $15^{\circ}C$.

• The Korean Journal of Food And Nutrition
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• v.32 no.5
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• pp.511-521
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• 2019

The purpose of this study was to investigate the inhibitory effect of enzyme activity and anti-proliferation of human cancer cell lines (HCT 116, NCI-H460 and MCF-7) of peanut skin depending on cultivars (Arachis hypogaea L. cv. K-Ol, cv. Sinpalkwang, cv. Daan, cv. Heuksaeng) and extraction solvent. Peanut skin was extracted with 80% ethanol, 80% methanol, 80% acetone, and distilled water, followed by analysis of the enzyme inhibitory activity and anticancer activity. Methanol extract of Daan cultivar most effectively inhibited ${\alpha}$-gluosidase (65.08%, 0.025 mg/mL), tyrosinase (82.49%, 2 mg/mL) and ACE (73.61%, 10 mg/mL). The inhibitory effect of peanut skin extracts on colon cancer cell (HCT-116), lung cancer cell (NCI-H460) and breast cancer cell (MCF-7) growth were investigate using MTT assay. The highest anti-proliferation of cancer cell line of peanut skin extracts was observed in the methanol extract of Daan cultivar. The cell viability on HCT 116, NCI-H460 and MCF-7 cell lines of methanol extracts from peanut skin of Daan cultivar was 48.13%, 41.03%, and 36.02% at $200{\mu}g/mL$, respectively. These results suggest that peanut skin extracts may mediate physiological activity, and provide valuable information for the use of peanut byproduct as a functional food material.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.52 no.1
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• pp.55-59
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• 2007

This experiments were conducted to evaluate suitable plant-type and cultivars for producing fresh pod peanut from late seeding as succeeding crop, compared with early seeding as proceeding crop or single cropping. 12 cultivars according to grain weight and plant types, 6 virginia typed cultivars(ssp. hypogaea) and 6 shinpung typed cultivars(ssp. fastigiata), were used for early and late seedings. The plant growth and yield potential in early seeding were better than those in late seeding. But the ratios of dry/fresh pod and of mature pod in late seeding were higher than those of early seeding. The yield of fresh pod by cultivars in two seeding times showed significant correlation with pod scale such as fresh pod weight, 100-grain weight, and dry seed yield positively, but pod number negatively in early seeding only. Yield of fresh peanut between Virginia and Shinpung types didn't show significant difference in early seeding, but showed in late seeding. Average yield of Virginia typed cultivars showed significantly higher than that of Shinpung typed ones. This yield gap between two plant types was the same tendency on extending seedings to July 20.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.65 no.4
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• pp.447-456
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• 2020

Peanut is an allotetraploid derived from a single recent polyploidization. Polyploidization has been reported to have caused significant loss in genetic diversity during the domestication of cultivated peanuts. Single nucleotide polymorphism (SNP)-based markers such as cleaved amplified polymorphic sequences (CAPS) derived from next-generation sequencing (NGS) have been developed and widely applied for breeding and genetic research in peanuts. This study aimed to identify the genetic diversity and population structure using 30 CAPS markers and 96 peanut accessions from five different origins. High genetic dissimilarities were detected between the accessions from Korea and those from the other three South American origins generally regarded as the origin of peanuts, while the accessions from Brazil and Argentina presented the lowest genetic dissimilarity. Based on the results of the present study, accessions from Korea have unique genetic variation compared to those from other countries, while accessions from the other four origins are closely related. Our study identified the genetic differentiation in 96 peanut accessions from five different origins, and this study also showed the successful application of SNP information derived from re-sequencing based on NGS technology.

• Korean Journal of Plant Resources
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• v.20 no.6
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• pp.553-557
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• 2007

The purpose of this study is to establish an extraction and analysis method for $\alpha$-tocopherol, and to then distinguish among varieties. The $\alpha$-tocopherol contents of 22 varieties of peanut seeds were analyzed by HPLC. Peanut seeds of cv. Palkwang were processed in two ways, by boiling and roasting. The $\alpha$-tocopherol contents of the two types of peanuts were compared with fresh seeds without any processing. $\alpha$-Tocopherol was detected at a retention time of 2.95 minutes. Five standards of $\alpha$-tocopherol covering a range of $20{\sim}100{\mu}g/ml$ were made up in 2% isopropyl alcohol/n-hexane and analyzed in duplicate. The $\alpha$-tocopherol contents differed according to extraction temperature. The contents were $85{\mu}g/g$ or less at $10^{\circ}C\;and\;20^{\circ}C$ and $94{\mu}g/g$ at $30^{\circ}C$, but they were decreased at $40^{\circ}C$ or higher. The $\alpha$-tocopherol contents in 22 peanut varieties were $61.36{\sim}96.80{\mu}g/g$ according to variety. Fresh peanuts contained $106.7{\mu}g/g$ of $\alpha$-tocopherol, while boiled peanuts contained $108.8{\mu}g/g$ of $\alpha$-tocopherol, and roasted peanuts contained $109.2{\mu}g/g$ of $\alpha$-tocopherol.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.45 no.5
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• pp.339-342
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• 2000

Near-infrared reflectance spectroscopy (NIRS) was used to estimate the lipid and protein contents in ground seed samples of perilla (Perilla frutescens Brit.) and peanut (Arachis hypogaea L.). A total of 46 perilla and 80 peanut calibration samples and 23 perilla and 46 pea. nut NIRS validation samples were used for NIRS equation development and validation, respectively. Validation of these NIRS equations showed a range of very low bias (-0.05 to 0.13 %) and standard error of prediction corrected for bias (0.224 to 0.803%) and very high coefficient of determination ($R^2$) (0.962 to 0.985). It was concluded that NIRS could be adapted as a mass screening method for lipid and protein contents in perilla and peanut seed.

• Korean Journal of Food Science and Technology
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• v.34 no.5
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• pp.924-926
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• 2002

The effects of inlet air temperature and atomizing pressure on the coating efficiency were evaluated using peanuts. Broken peanut pieces were coated with dextrin and sodium caseinate solution by a fluidized bed coater. The coating efficiency was significantly influenced by inlet air temperature and atomizing pressure, with the optimal efficiency achieved at $70^{\circ}C$ and 3 bar, respectively. The coating material consisting of dextrin and sodium caseinate could be used for preventing rancidity of broken peanut.

• Research in Plant Disease
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• v.28 no.1
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• pp.54-56
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• 2022

A peanut plant showing wilt and browned symptom was found in the field of Gochang, Korea, in July 2021. The symptomatic peanut plant was collected from the field and isolation of the pathogen caused the wilt symptom was performed using the collected sample on TZC media. The dominated colony on media was isolated colony on media was isolated and subcultured of purification. The pure cultured bacteria was identified as Ralstonia solanacearum by sequencing of 16S rRNA gene. Multiplex polymerase chain reaction using phylotype-specific primer set identified isolate as phylotype I (R. pseudosolanacearum). Phylogenetic tree was constructed based on 16S rRNA sequence and it was closed with R. pseudosolanacearum. Pathogenicity of the isolates was assessed by soil drenching inoculation on 4-week-old peanut plant. The wilt symptom was successfully reproduced by inoculation of the isolates after 14 days. This is first report of bacterial wilt caused by R. pseudosolanacearum on peanut in Korea.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.113-113
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• 2022

Salinity in surface waters is increasing around the world. Many factors, including increased water extraction, poor irrigation management, and sea-level rise, contribute to this change, and posing a threat to plant development and agricultural production. Seeds exposed to high salinity, have a lower probability of germinating and various physiological and biochemical effects. Salinity stress affects more than 20% of agricultural land and about 50% of irrigated land. In the current study, our objective is to identify the salt-tolerant peanut (Arachis hypogaea L.) Korean genotypes under salinity stress. Thus, two-week-old 19 diverse peanut Korean genotypes were exposed to 10 days of salinity (150 mM NaCl) stress. Based on the growth attributes investigation, Baekjung and Ahwon genotypes showed significantly higher shoot lengths compared to control plants. Whereas, the Sinpalwang genotype exhibited a significantly positive response for plant growth and reduced wilting symptoms compared to other genotypes. This study was able to find out peanut tolerant and sensitive genotypes for salt stress. These results may provide a good template for further salt-tolerant peanut cultivar improvement programs. Identified diverse salt-responsive genotypes can be utilized as source material in Korean breeding schemes for peanut crop improvement for salt and other abiotic stress tolerance.