• Analytical Science and Technology
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• v.9 no.1
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• pp.112-117
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• 1996

Three active compounds were isolated from the culture of a plant pathogenic fungus, Bipolaris cynodontis. The structure elucidation of these compounds was accomplished by 2D NMR techniques, such as $^1H-^1H$ and $^{13}C-^1H$ COSY, COLOC, HMBC and rotating frame NOE(ROESY). Compounds were found as derivatives of cochlioquinone and cochlioquinol that were previously isolated as phytotoxins from B. bicolor and B. cynodontis, respectively. The compounds showed phytotxicity against Italian ryegrass, one of the host plants of B. cynodontis.

• The Plant Pathology Journal
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• v.24 no.2
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• pp.202-206
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• 2008

Antifungal activity of Korean and Chinese lichen-forming fungi (LFF) was evaluated against plant pathogenic fungus of Colletotrichum acutatum, causal agent of anthracnose on hot pepper. This is the first attempt to evaluate antifungal activity of LFF, instead of lichen thalli, against C. acutatum. Total 100 LFF were isolated from the lichens with discharged spore method or tissue culture method. Among the 100 isolates, 8 LFF showed more than 50% of inhibition rates of mycelial growth of the target pathogen. Especially, Lecanora argentata was highly effective in inhibition of mycelial growth of C. accutatum at the rate of 68%. Antifungal activity of other LFF was in the order of Cetrelia japonica (61.4%), Ramalina conduplicans (59.5%), Umbilicaria esculenta (59.5%), Ramalina litoralis (56.7%), Cetrelia braunsiana (56.5%), Nephromopsis pallescensn (56.1%), and Parmelia simplicior (53.8%). Among the tested LFF, 61 isolates of LFF exhibited moderate antifungal activity against the target pathogen at the inhibition rates from 30 to 50%. Antifungal activity of the LFF against C. acutatum was variable at the species level rather than genus level of LFF. This study suggests that LFF can be served as a promising bioresource to develop novel biofungicides.

• Mycobiology
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• v.35 no.1
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• pp.39-43
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• 2007

The antifungal effect of pine needle extract prepared by a distinguishable extraction method and the dry distillation method, was examined. The effect of this extract itself was insignificant. The chemical components of pine needle extract were then investigated by gas chromatographic analysis, and four, chemical components, acetol, furfural, 5-methyl furfural, and terpine4-ol, were identified. The antifungal effects of those four chemical components against Alternaria mali (A. mali), an agent of Alternaria blotch of apple, were then examined. It was observed that the minimum inhibitory concentrations (MICs) were 6.25, 0.78, 0.78, and 12.5 (mg/ml) of acetol, furfural, 5-methyl furfural, and terpine-4-ol, respectively. MICs of furfural and 5-methyl furfural had the same order of magnitude as that of an antifungal agrochemical, chlorothalonil. Although furfural itself can not be completely substituted for an antifungal agrochemical, a partial mixture of furfural and antifungal agrochemical may be used as a substitute. The use of agrochemicals for the prevention of plant disease caused by pathogenic fungus such as A. mali could be partially reduced by the application of this mixture.

• International Journal of Industrial Entomology and Biomaterials
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• v.14 no.2
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• pp.81-85
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• 2007

White Muscardine is the most common fungal disease of silkworm, Bombyx mori L. caused by the pathogenic fungus, Beauveria bassiana (Balsamo) Vuillemin. In the present investigation, an attempt has been made to screen locally available medicinal/ weed plants against Beauveria bassiana. Among the plant extracts (PE) tested, 5% aqueous crude extract of the bulb of Allium sativum (Garlic) has been found to be most effective against Beauveria bassiana. The radial growth of Beauveria bassiana in vitro was inhibited to the tune of 54.9% in aqueous extract and 54.4% in ethanolic extract of Allium sativum and correspondingly mycelial dry weight gave rise to 110.7 mg and 108.7 mg against 201.7 mg in control 15 days post treatment. Similarly, silkworm larvae topically inoculated with the Beauveria bassiana conidia ($1.8{\times}10^6/ml$) registered survival up to 53.0% against 0.0% in control after treatment with aqueous extract of Allium sativum. Simultaneously, as a preventive measure, silkworm larvae were put to rear in conidia contaminated seat paper instantly treated with aqueous extract of Allium sativum that also increased survival up to 61.0% against 4.6% in control. It is also observed that the plant extract is absolutely innocuous to silkworm.

• Journal of Korean Society of Forest Science
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• v.6 no.1
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• pp.6-10
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• 1967

In the present paper author investigated the symptom, pathogenic fungus and pathogenicity of Endothia canker of Carpinus laxiflora in Korea, and made clear the indistinct discription on its pathogen in the past. 1. The pathogen is identified as Endothia fluens (Schw.) Shear et Stevens. The discription is recorded as follows: Stromata cortical, erumpent, spherical or conical, outer yellowish-brown and inner yellowish, 0.5 to 2.5 mm in diameter; perithecia irregularly embeded in the bottom of stroma, 7 to 23 in a stroma usually spherical to elliptical or irregular, 235 to $370{\mu}$ in diameter, with black slender necks; each neck open the papilliate ostiole to the surface, about 250 to $400{\mu}$ in length; asci clavate or fusoid, colorless, 31.16 to 42.64 by 6.54 to $8.20{\mu}$ in size, average 37.02 by $6.84{\mu}$, with 8 ascospores in double line; ascospores elliptical, ovate or cylindrical, with rounded ends, hyaline, 1-septate, not constrict at the septum, 6.51 to 9.30 by 3.16 to $3.72{\mu}$, average 7.61 by $3.44{\mu}$ in size; pycnidia formed abundantly in stroma. spherical at first but later irregular large cavity by fussing each other; pycnospores oblong or rod-shaped, hyaline, non-septate, 3.8 by $1.9{\mu}$ in size; spore-horn formed abundantly under moist condition. 2. The pathogen is wound parasite invading the hosts through the wound. But after the infection is established, expanding the disease lesion is swiftly vigorus.

• Journal of Life Science
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• v.19 no.3
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• pp.343-348
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• 2009

Magnaporthe oryzae, a major cause of rice blast, is one of the most destructive plant fungal pathogens. Secretion of reactive oxygen species (ROS) during the infection phase of plant pathogenic fungus plays a key role in the defense mechanism of a plant. ROS causes oxidative damage and functional modification to the proteins in a pathogenic fungus. Methionine, especially, is a major target of ROS, which oxidizes it to methionine sulfoxide. To survive from the attack of ROS, plant pathogenic fungus has antioxidative systems - one example would be methionine sulfoxide reductase B (MSRB), which reverses the oxidative alteration of methionine to methionine sulfoxide. In the present study, identification and molecular characterization of the MSRB gene in M. oryzae KJ201 were investigated. The MSRB gene was amplified by PCR from the M. oryzae KJ201 genomic DNA. The copy number of MSRB in the genome of M. oryzae KJ201 was identified by Southern blot analysis, which revealed that the gene exists as a single copy. To study the molecular function of an MSRB gene, the expression level of the MSRB gene was assayed with hydrogen peroxide treatment by Northern blot analysis and RT-PCR. The expression of the MSRB gene was increased by treatment of hydrogen peroxide, without significant correlation to hydrogen peroxide concentrations. These results indicate that the MSRB gene in M. oryzae KJ201 could contribute to protection against plant defense compounds such as ROS and offer a novel strategy for the control of rice blast.

• Mycobiology
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• v.29 no.2
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• pp.110-112
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• 2001

A black scab was observed on strawberry(Fragaria ananassa) in plastic film houses around Jinju area during the winter of 2000. The disease started from leaves then moved to calyx and runner. At the beginning, the infected area started with small dark brown spots then gradually expanded. The pathogenic fungus was purely isolated from the diseased leaves, calyx and runner. The fungus was inoculated to test Koch's postulates and proved to be the causal agent of the disease. The isolated fungus grew readily on potato dextrose agar, forming dark green to dark gray colonies. The optimum temperature for mycelial growth was about $25^{\circ}C$. The diameter of growing hyphae was $3.8{\sim}5.6{\mu}m$. Conidia were ellipsoidal, ovoid or subspherical, mostly one-celled but occasionally septate. The size of conidia were $4.1{\sim}11.7{\times}3.8{\sim}5.3$ 1-cell, $9.3{\sim}18.8{\times}4.0{\sim}7.4{\mu}m$ 2-cell and formed in long branched chains on the erected conidiophores which were dark brown and variable in length between $28.8{\sim}236.2{\times}3.0{\sim}6.2{\mu}m$ in size. The fungus was identified as Cladosporium herbarum on the basis of its morphological characteristics. The black scab disease of strawberry caused by C. herbarum has not been reported in Korea previously.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.69.2-70
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• 2003

For the selection and breeding of chestnut varieties resistant to the chestnut blight fungus Cryphonectria parasitica, disease resistance and susceptibility of 28 varieties widely planted and growing in Korea were evaluated by artificial inoculation of a pathogenic fungus. For this experiment, a typical virulent strain (KCPC-19) was selected. Artificial inoculation was conducted into all varieties by using two different materials and methods, i.e., bark and wood tissue sections in the laboratory and living trees in the field. In the bark and wood tissue section method, the size of necrotic area and canker development on chestnut varieties were examined and compared 4 days after inoculation. There were wide variations of chestnut varieties in disease resistance and susceptibility against chestnut blight fungus, but 3 varieties, Daebo！, Ishizuchi, and Sandae, were shown to be relatively resistant to the disease with the necrotic area of 0.95-1.03 cm2, while Arima was the most susceptible with the size of 2.0 cm2. In the living tree inoculation examined 5 weeks after inoculation, 3 varieties, Daebo, Ishizuchi, and Riheiguri, showed the higher resistance, but Tono 2 did the highest susceptibility among tested varieties.

• The Plant Pathology Journal
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• v.15 no.6
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• pp.345-347
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• 1999

A scab disease on eggplant (Solanum melongena L. cv. Chukyang) in plastic film houses around Kimhae area in Korea during the winter season of 0998-1999. The disease started on leaves with small dark brown spots which were gradually expanded to 1 to 3 mm diameter lesions. Later, the central parts of the lesions became collapsed and detached to make holes. Dark brown mold was grown out of the lesions on the lower side of leaf. Numerous conidia were produced on the lower side of leaf. Numerous conidia were produced on the diseased leaves and appeared to be readily dispersed in the air. A fungus was isolated from the diseased leaves, and tested for Koch's postulates to prove the causal agent of the desease. The isolated fungus grew on potato dextrose agar, forming greenish black to pale brown colonies. Conidia were ellipsoidal, fusiform or subspherical, mostly one-celled but occasionally septated, and formed in long branched chains on the erected conidiophores which were pale olevaceous brown and variable in length between 12.4 and $393.4\mu\textrm{g}$. The fungus was identified as Cladosporium cucumerinum Ellis Arthur based on the above morphological characteristics examined. The optimum temperature for mycelial growth and conidial formation was about 20 to $25^{\circ}$. In addition to cucumber, the fungus was also pathogenic to watermelon, pumpkin and oriental melon. This is the first report on the scab disease of eggplant in Korea.

• Korean Journal Plant Pathology
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• v.14 no.5
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• pp.458-462
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• 1998

Stem rot symptomof lily caused by Phytophthora was observed in Chilkok, Korea in 1997. Oogonia of Phytophthora were abundantly developed on epidermal tissues of stem and leaves of the diseased plants. The casual fungus was identified as Phytophthora cactorum based on their cultural and morphological characteristics. Sporangia were markedly papillate and the shape was broadly spherical to ovoid both on agar and in water. The size was 28~44$\times$20~28 ${\mu}{\textrm}{m}$ (av. 36~24) and a short pedicel was attached to each sporangium. The fungus was homothallic and oogonia were 28~32 ${\mu}{\textrm}{m}$ (av. 30) in size and slightly yellow. Antheridia were paragynous and spherical or irregularly club shaped. Optimal temperature for mycelial growth was 25~27$^{\circ}C$. The fungus did not grow under 6$^{\circ}C$ and over 34$^{\circ}C$. The fungus was highly pathogenic to three cultivars of lily. Symptom was developed at 4-day after artificial inoculation with zoospores (103 cell/ml) on the stem of lily. The symptom was identical to those produced by natural infection in the field and the plant was blighted within 14 days after inoculation.