• Clinical and Experimental Reproductive Medicine
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• v.35 no.1
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• pp.39-48
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• 2008

Objective: The aim of this study was to investigate whether multipotent germline stem cells (MGSCs) can be established from neonatal mouse testis. Methods: Various cells containing MGSCs were collected from neonatal testis of ICR mice and allocated to plates for in vitro culture. After 7 days in culture, the cells were passed to a fresh culture plate and continuously cultured. From the third or fourth passage, the presumed MGSCs were cultured and maintained on mitomycin C-inactivated STO feeder cells. The MGSCs were cultured in a condition where mouse embryonic stem cells (ESCs) are cultured. Characteristics of the MGSCs were evaluated by RT-PCR, immunocytochemistry, alkaline phosphatase activity, karyotyping, and transmission electron microscopy. Results: Two MGSCs lines were established from 9 pooled sets of neonatal testicular cells. MGSCs colonies were morphologically undistinguishable from ESCs colonies and both MGSC lines as well as ESCs expressed undifferentiated stem cell markers, such as Thy-1, Oct-4, Nanog, Sox2 and alkaline phosphatase. Fine structure of undifferentiated MGSCs were similar to those of ESCs and 60% of MGSCs (12/20) had normal karyotype at passage 10. They were able to form embryoid bodies (EBs) and MGSC-derived EBs expressed marker genes of three germ layers. Conclusion: We could establish the MGSCs from neonatal mouse testis and they were differentiated to multipotent lineages of three germ layers. Molecular characteristics of MGSCs were similar to those of ESCs. Our results suggest a possibility that multipotent stem cells derived from testis, the MGSCs, could replace the ESCs in biotechnology and regenerative medicine.

• Proceedings of KOSOMES biannual meeting
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• 2007.11a
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• pp.169-174
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• 2007

A huge marine accident causes a loss of valuable lives, property and the serious environmental contamination. In the IMO, active discussions about a potential occurrence possibility of marine accidents and appropriate place(place of refuge) to minimize the damage are being developed as appropriate transferring vessels which has a worry of a secondary marine environmental pollution from a previous marine accident Our country has a great potential et marine accidents due to the foreign vessel in distress because there is a huge vessel traffic amounts of the passage since which locate with in the important navigation connects the Asian region. and North America centering around north Pacific Ocean Also, we need the minimize the secondary damage from accident which occurs in domestic ports as transferring safe쇼 place when it is needed In this paper, analyzed and investigated a designated the place of refuge that analyze foreign cases, and will make a efficient and reasonable investigating plan of interior place of refuge.

• Journal of Gastric Cancer
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• v.17 no.1
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• pp.11-20
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• 2017

Purpose: Acupuncture has recently been accepted as a treatment option for managing postoperative ileus (POI) and various functional gastrointestinal disorders. Therefore, we conducted a prospective randomized study to evaluate the effect of acupuncture on POI and other surgical outcomes in patients who underwent gastric surgery. Materials and Methods: Thirty-six patients who underwent distal gastrectomy for gastric cancer from March to December 2015 were randomly assigned to acupuncture or non-acupuncture (NA) groups at 1:1 ratio. The acupuncture treatment was administered treatment once daily for 5 consecutive days starting at postoperative day 1. The primary outcome measure was the number of remnant sitz markers in the small intestine on abdominal radiograph. The secondary outcome measure was the surgical outcome, including the times to first flatus, first defecation, start of water intake, and start of soft diet, as well as length of hospital stay and laboratory findings. Results: The acupuncture group had significantly fewer remnant sitz markers in the small intestine on postoperative days 3 and 5 compared to those in the NA group. A significant difference was observed in the numbers of remnant sitz markers in the small intestine with respect to group differences by time (P<0.0001). The acupuncture group showed relatively better surgical outcomes than those in the NA group, but the differences were not statistically significant. Conclusions: In this clinical trial, acupuncture promoted the passage of sitz markers, which may reflect the possibility of reducing POI after distal gastrectomy.

• Proceedings of the KIEE Conference
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• 2009.07a
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• pp.1070_1071
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• 2009

Recently, small hydropower attracts attention because of its clean, renewable and abundant energy resources to develop. Therefore, a cross-flow hydraulic turbine is proposed for small hydropower development in this study. The turbine‘s simple structure and high possibility of applying to the sites of relatively low effective head and large flow rate can be advantages for the introduction of the small hydropower development. The purpose of this study is not only to investigate the effects of air layer in the turbine chamber on the performance and internal flow of the cross-flow turbine, but also to suggest a newly developed air supply method. CFD analysis for the performance and internal flow of the turbine is conducted by an unsteady state calculation using a two-phase flow model in order to embody the air layer effect on the turbine performance effectively. The result shows that air layer effect on the performance of the turbine is considerable. The air layer located in the turbine runner passage plays the role of preventing a shock loss in the runner axis and suppressing a recirculation flow in the runner. The location of air suction hole on the chamber wall is very important factor for the performance improvement. Moreover, the ratio between air from suction pipe and water from turbine inlet is also significant factor of the turbine performance.

• International Journal of Advanced Culture Technology
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• v.4 no.4
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• pp.45-50
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• 2016

This is a humanistic study to trace phenomena logically the comprehensive therapeutic mechanism of the human body which is coded by the smart emotion of the rated signal conveyed by the Rated Sijo. The Gestalt, which is structured in the form of therapeutic metastasis conveyed by sentences, is intended to embody the principle of human response. So, this researcher explored the metastatic structure toward Gestalt of original human being through the passage of foreground and background by ergonomic and chemical structure. In the meantime, this researcher focused on revealing the structure of the field of existence by the symbol system in which the therapeutic mechanism of the human body is embodied. As a result, the basic framework of Gestalt literary therapy, which contributes to the improvement of the Quality of Life metaphorized as a mechanism of the symbol system by the metastasis of literary therapy or the electrical operation of the human body. As a result, the human body as a conductor through literature has turned out to be an original Gestalt structure pursued by literature. In addition, it was analyzed that the human body would accept signals such as emotions and Rated Emotions planted in the sentence, and synapse them into the human physiological psycho analytical symbol system. Therefore, it has been confirmed possibility that human existent environment and trauma are separated from the whole universe can push fully implement therapeutic techniques toward totalization by a combination of literary devices, especially appropriate electric signal combination of the Rated Sijo.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.26 no.11
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• pp.1559-1569
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• 2002

In order to investigate the characteristics of electromagnetic flowmeter in two -phase flow, an AC electromagnetic flowmeter was designed and manufactured. In various flow conditions, the signals and noises from the flowmeter were obtained and analyzed by comparison with the observed flow patterns with a high speed CCD camera. The experiment with the void simulators in which rod shaped non-conducting material was used was carried out to investigate the effect of the bubble position and the void fraction on the flowmeter. Based on the results from the void simulator, two -phase flow experiments encompassed from bubbly to slug flow regime were conducted. The simple relation $\Delta$ $U_{TP}$ = $\Delta$ $U_{SP}$ (l-$\alpha$) was verified with measurements of the potential difference and the void fraction. Due to the lack of homogeneity in a rent two -phase flow, the discrepancy between the relation and the present measurement was slightly increased with void fraction and also liquid volumetric flux jf. Whereas there is no difference in the shape of the raw signal between single-phase flow and bubbly flow, the signal amplitude for bubbly flow is higher than that for single -phase flow at the same water flow rate, since the passage area of the water flow is reduced. In the case of slug flow, the phase and the amplitude of the flowmeter output show dramatically the flow characteristics around each slug bubble and the position of a slug bubble itself. Therefore, the electromagnetic flowmeter shows a good possibility of being useful for identifying the flow regimes.ul for identifying the flow regimes.

• The Plant Pathology Journal
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• v.24 no.3
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• pp.289-295
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• 2008

RNA-RNA interactions and the dynamic RNA conformations are important regulators in virus replication in several RNA virus systems and may also involved in the regulation of many important virus life cycle phases, including translation, replication, assembly, and switches in these important stages. The 5' non-translated region of Potato virus X(PVX) contains multiple cis-acting elements that facilitate various viral processes. It has previously been proposed that RNA-RNA interactions between various RNA elements present in PVX RNA genome are required for PVX RNA accumulation(Hu et al., 2007; Kim and Hemenway, 1999). This model was based on the potential base-pairing between conserved sequence elements at the upstream of subgenomic RNAs(sgRNAs) and at the 5' and 3' end of RNA genome. We now provide more evidence that RNA-RNA base-pairing between elements present at the 5' end and upstream of each sgRNA is required for efficient replication of genomic and subgenomic plus-strand RNA accumulation. Site-directed mutations introduced at the 5' end of plus-strand RNA replication defective mutant(${\Delta}12$) increasing base-pairing possibility with conserved sequence elements located upstream of each sgRNAs restored genomic and subgenomic plus-strand RNA accumulation and caused symptom development in inoculated Nicotiana benthamiana plants. Serial passage of a deletion mutant(${\Delta}8$) caused more severe symptoms and restored wild type sequences and thus retained possible RNA-RNA base-pairing. Altogether, these results indicate that the RNA element located at the 5' end of PVX genome involved in RNA-RNA interactions and play a key role in high-level accumulation of plus-strand RNA in vivo.

• Korean Institute of Interior Design Journal
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• v.13 no.5
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• pp.106-113
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• 2004

The purpose of this study is to present the dwelling space by attaching or inserting the mobile space unit to the fixed building by providing the temporary dwelling space for those suffering from flood disaster, natural disaster or solitary old people or the space and program that the completed building in the city can not provide such as temporary events in the bridge, passage space, walking space for the handicapped, stores located between street blocks, and plaza. Therefore, this study was intended to investigate temporary dwelling space through the composition of space and the experiment of the applicability of the program through the methods of movement, folding, module, expansion and evolution of mobile space. An attempt was made to apply the concept of ‘mobile space’ to the dwelling space not only with a focus on the freedom of variable and changeable physical movement but also with a focus on the existence of the nomad and the way of thinking that bleaks away from a given value system and code, creates a new value and continues to move to a new domain. This study attempted to analyze how the concept of ‘mobile space’ has been developed in what form and method they have been applied and interpreted in the historical background. It attempted to resolve the concepts of movement and mobility through the experiment so that they might apply to the space of contemporary city. This study attempted to experiment this possibility using the mobile space unit and apply it.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.27 no.4
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• pp.333-351
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• 2017

Objectives: This study examines the relationships between indoor radon concentrations and distribution from soil geological mapping in the Hwacheon and Jangsu areas. Methods: GIS and a pivot table were used for inquiries about indoor radon contents, soil characteristics, and geological differences. Results: The Hwacheon area was characterized by the presence of normal and reverse faults as a passage of runoff for radon, sufficient occurrences of minerals containing uranium within granite as a radon source, a high concentration of radon within the granite area and clear differences of radon concentrations between granitic and metamorphic areas. The Jangsu area was characterized by the presence of normal faults, wide distributions of alluvium, and ambiguities on radon concentrations indoors among areas of geological differences. Considering the granite area and alluvium surrounded with granite areas, the characteristics of radon concentrations within soils and indoors in the Jangsu area are similar to those of the Hwacheon area. High concentrations are found with entisol and inceptisol in the Hawcheon area, but with entisol, inceptisol, and ultisol in the Jangsu area. High radon concentrations are found in sandy loam and/or loam. High concentrations are found in recently constructed or brick buildings, but low concentrations in traditional or prefabricated houses showing a high possibility of outward flow. Conclusions: The overall results suggest that radon concentrations in the Hwacheon and Jangsu area are dominantly influenced by geological characteristics with additional artificial influences.

• Journal of The Korean Society of Grassland and Forage Science
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• v.25 no.1
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• pp.65-70
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• 2005

This study was conducted to investigate the effect of combine crawler attaching slurry spreader on soil and growth of rye. The performance test at actual field was conducted to test for possibility of practical use. Field test of the slurry spreader was operated in upland and paddy field. Experiments was conducted to compare the effects of the soil damage and crop productivity incorporating Pig slurry by two different types of slurry spreader. In this experiment a slurry spreader using crawler of combine have been designed and developed to enhance the operation in small field and reduce e damage of soil structure. but it was not suitable for transference in long distance. Conventional tractor mounted vacuum is not suitable for $71.6\%$ water condition in the small areas of paddy land. But the slurry spreader using crawler could be controlled the working point and moving by crawler in paddy land of small area. The wheels depths of soil in the supplementary application in the rye field was 4.9 m in tractor mounted spreader. but the wheels depth of crawler attaching spreader was 1.6cm. The dry matter yield of rye was reduced by $12\%$ in supplementary application of slurry due to mechanical damage from passage of the tires. This is particularly noticeable at high soil moisture condition in paddy land. It was concluded that the slurry spreader developed in this study could be successfully used for basal and supplementary application of slurry in rye paddy field.