• 제목/요약/키워드: Park je sang

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취나물 점무늬병을 억제하는 Paenibacillus polymyxa JE201의 생물학적 특성 (Biological Characterization of Paenibacillus polymyxa JE201 with Antifungal Activity Against Fungal Leaf Spot Disease of Aster scaber)

  • 안성호;김다연;박병용;한지희;이상엽
    • 한국유기농업학회지
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    • 제29권2호
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    • pp.257-273
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    • 2021
  • 취나물 재배 시 문제가 되는 점무늬병(Septoria sp.)에 대해 항균활성을 나타내는 세균 P. polymyxa JE201를 분리하였다. JE201 균주를 이용하여 점무늬병 방제효과 포장검정을 시행한 결과, 대조구로 사용한 세레나데 맥스와 비슷한 정도의 방제효과를 나타낼 정도로 효과가 있었으며, 다른 식물 병원성 진균에 대한 항균활성 스펙트럼을 조사한 결과, 10종 중 9종에 대해서도 균사생장억제력이 크게 나타나는 것으로 보아, 앞으로 추가적인 연구를 통해 다른 병에도 적용할 수 있을 것으로 기대된다.

Study on Persistent Infection of Japanese Encephalitis Virus Beijing-l Strain in Serum-free Sf9 Cell Cultures

  • Kim, Hun;Lee, Su-Jeen;Park, Jin-Yong;Park, Yong-Wook;Kim, Hyun-Sung;Kang, Heui-Yun;Hur, Byung-Ki;Ryu, Yeon-Woo;Han, Sang-In
    • Journal of Microbiology
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    • 제42권1호
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    • pp.25-31
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    • 2004
  • Sf9 cells have obvious advantages for the conventional production technology of vaccine. They are useful tools for high concentration and large-scale cultures. Sf9 cells were grown to maximal concentration, 8${\times}$l0$\^$6/ cells/$m\ell$ in a 500$m\ell$ spinner flask, with a doubling time at the exponentially growing phase of 24.5 hours, using serum-free media. To explore the ability of Sf9 cells to be infected by the Japanese encephalitis (JE) virus Beijing-l strain, Sf9 cells were infected with the virus. By 4-5 days post-infection, 10-15 % of the Sf9 cells showed cytopathic effect (CPE), from granularity to the formation of syncytia and multinucleated giant cells continuously observed over a period of 35 days. Positive fluorescent reactions were detected in 30-40% of cells infected with the JE virus Beijing-l strain, and the uninfected Sf9 cells were completely negative. Virus particles, propagated in Sf9 and Vero cells, were concentrated by sedimentation on 40% trehalose cushions by ultracentrifugation, and showed identical patterns of viral morphogenesis. Complete virus particles, 40 to 50 nm in diameter, were observed, and JE virus envelope (E) proteins, at 53 kDa, were found in the western blot analysis to the anti-JE virus E protein monoclonal antibody and reacted as a magenta band in the same position to the glycoprotein staining. To evaluate whether the infectious virus was produced in Sf9 cells inoculated with the JE virus Beijing-l stain, Sf9 cells were inoculated with the virus, and sample harvested every 5 days. The titers of the JE virus Beijing-l strain rose from 1.0${\times}$l0$\^$5/ to 1.5${\times}$l0$\^$6/ pfu/$m\ell$. The infected Sf9 cells could be subcultured in serum-free medium, with no change in the plaque sizes formed by the JE virus Beijing-l strain in the plaque assay. It is suggested that the ability of the JE virus Beijing-l strain to infect Sf9 cells in serum-free media will provide a useful insect cell system, where the JE virus replication, cytopathogenicity and vaccine immunogen can be studied.

대순사상에서의 무극(無極)과 태극(太極) 그리고 대순(大巡)의 의미 고찰 (A Study on the Meaning of Mugeuk(無極) and Taegeuk(太極) in Daesoon Thoughts)

  • 박재현
    • 대순사상논총
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    • 제22권
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    • pp.433-469
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    • 2014
  • Identifying the ontological characteristics of "ultimate reality" is highly important in a specific religion or thought because it is believed to contain the logic that explain the wholistic phenomenon in the entire universe. The "ultimate reality" is called "Lee(理)", "Dao(道)", "Taegeuk; the Great Ultimate(太極)", "Mugeuk; NonUltimate(無極)", "Gong(空)", "Haven", "SangJe(上帝)", "God" in many different religions or thoughts. Taegeuk(太極), Mugeuk(無極) and Daesoon(大巡) are believed to contain the logic that explain the wholistic phenomenon in the entire universe in Daesoon Thoughts which we can see in the GooCheon SangJe(九天上帝)'s saying that "Even if the logic is so high, it comes from the surface of Taegeuk and Mugeuk and cannot be separated from every phenomenon in our life(理雖高出於太極无極之表 不離乎日用事物之間)" and Doju(道主) Cho, Jeong San's saying that "The reasion that Dao is called Dao is that it is settled to be Mugeuk and moved to be Taegeuk(道之謂道也者定而无極 動而太極)" and Dojeon(都典) Park Wu-dang's saying that "Daesoon(大巡) is circle(圓), circle is Mugeuk(無極), Mugeuk is Taegeuk(太極)". Mugeuk contains the logic of transcendence and Taegeuk contains the logic of immanance. And Nae-confucianism admit the Ultimate Reality or logic have both characteries of Mugeuk and Taegeuk and don't admit that Taegeuk comes from Mugeuk. Therefore, Mugeuk and Taegeuk are in complementary relations. These Nae-confucianism's idea about Mugeuk and Taegeuk corresponds with Deasoon Thought. Daesoon Thoughts also admit that Ultimate Reality, GooCheon SangJe can be understood with both Mugeuk and Taegeuk. That is, GooCheon SangJe, the Suprime God is transcendent from this world(Mugeuk) and also manage the whole universe using the logic of Taegeuk(太極). Daesoon(大巡) unite Mugeuk and Taegeuk using the concept of circulation. The logic of Mugeuk and Taegeuk is both two as well as one, and one as well as two. It is not dualism nor monism. It is non-dualism and super-dualism. These logic of coexistence is the core of Mugeuk and Taegeuk. And this logic is reflexed in Daesoon Thoughts' core proposition that "Daesoon(大巡) is circle (圓), circle is Mugeuk(無極), Mugeuk is Taegeuk(太極)".

유사측도에 기반한 퍼지 엔트로피구성 (Fuzzy Entropy Construction based on Similarity Measure)

  • Park, Wook-Je;Park, Hyun-Jeong;Lee, Sang-H
    • 한국지능시스템학회:학술대회논문집
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    • 한국지능시스템학회 2007년도 추계학술대회 학술발표 논문집
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    • pp.366-369
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    • 2007
  • In this paper we derived fuzzy entropy that is based on similarity measure. Similarity measure represents the degree of similarity between two informations, those informations characteristics are not important. First we construct similarity measure between two informations, and derived entropy functions with obtained similarity measure. Obtained entropy is verified with proof. With the help of one-to-one similarity is also obtained through distance measure, this similarity measure is also proved in our paper.

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RFID 태그 인프라에서의 RFID 리더의 송출전력 제어를 통한 위치 추정 성능 향상 (Improvement of RFID localization performance using transmission power control in RFID tag infrastructure)

  • 박영수;박지훈;이제원;김상우
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 2008년도 제39회 하계학술대회
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    • pp.1815-1816
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    • 2008
  • 본 논문에서는 RFID 태그 인프라 혹은 RFID 태그 플로어 상에서 RFID 리더를 이용한 위치추정 시에 RFID리더의 송출전력을 제어하여 위치추정 성능을 향상시키는 방법을 제시한다. 또한 RFID 위치 추정 문제에 대하여 정의하고 이들의 성능을 비교하는 성능 기준 (Performance measure)을 이용하여 송출전력을 제어할 경우와 그렇지 않은 경우의 위치추정 성능을 시뮬레이션과 실험을 통해서 비교한다.

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