Korean Journal of Organic Agriculture (한국유기농업학회지)

Korean Association of Organic Agriculture (한국유기농업학회)
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Biological Characterization of Paenibacillus polymyxa JE201 with Antifungal Activity Against Fungal Leaf Spot Disease of Aster scaber

취나물 점무늬병을 억제하는 Paenibacillus polymyxa JE201의 생물학적 특성

  • 안성호 (국립농업과학원 농업미생물과) ;
  • 김다연 (농촌진흥청 국립농업과학원 환경개선미생물연구단) ;
  • 박병용 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 한지희 (농촌진흥청 국립농업과학원 기획조정과) ;
  • 이상엽 ((사)한국농자재시험연구기관협회)
  • Received : 2021.01.31
  • Accepted : 2021.05.14
  • Published : 2021.05.31
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Abstract

Koreans consume cham-chwi (Aster scaber thunb.) as a common vegetable in a meal because of its bitter taste and rich flavor. In addition, it is the crop with the most residual pesticides detected in the last five years. Among the detected pesticides, the most common was azoxystrobin, which is a drug used primarily to prevent the leaf spot disease of A. scaber caused by Septoria sp.. We isolated the microorganisms that antifungal activity against Septoria sp.. The optimum incubation conditions (temperature, pH and growth medium) were examined for the growth of the isolates. Additionally, cellulase and protease activity and siderophore production ability were also examined. According to 16S rRNA sequencing of the isolate was affiliated to Paenibacillus polymyxa JE201. Largest inhibition zone measuring up to 9.2 mm was observed for P. polymyxa JE201 after 7 days of inoculation. P. polymyxa JE201 strain showed antifungal activity against various fungal phytopathogens Altanaria sp., Botrytis cinerea, Colletotrichum acutatum, Fusarium oxysporum, Phytophthora capsici, Ph. drechesleria, Rhizoctonia solani and Stemphylium sp.. Based on these observations, P. polymyxa. JE201 can be used as a promising biocontrol agent for preventing the leaf spot disease and other phytopathogens.

취나물 재배 시 문제가 되는 점무늬병(Septoria sp.)에 대해 항균활성을 나타내는 세균 P. polymyxa JE201를 분리하였다. JE201 균주를 이용하여 점무늬병 방제효과 포장검정을 시행한 결과, 대조구로 사용한 세레나데 맥스와 비슷한 정도의 방제효과를 나타낼 정도로 효과가 있었으며, 다른 식물 병원성 진균에 대한 항균활성 스펙트럼을 조사한 결과, 10종 중 9종에 대해서도 균사생장억제력이 크게 나타나는 것으로 보아, 앞으로 추가적인 연구를 통해 다른 병에도 적용할 수 있을 것으로 기대된다.

Keywords

Acknowledgement

본 연구는 국립농업과학원 연구과제(과제번호 PJ012595)의 연구에 의해 이루어졌습니다.

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