Korean Journal of Organic Agriculture (한국유기농업학회지)

Korean Association of Organic Agriculture (한국유기농업학회)
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Effects of Additional Levels of Phyllostachys bambusoides on Ruminal Fermentation Characteristics and Methane Emission in in vitro

왕대의 첨가수준이 반추위 in vitro 발효성상과 메탄 발생량에 미치는 영향

  • 조성욱 (경상국립대학교 응용생명과학부(BK21)) ;
  • 이신자 (경상국립대학교 농업생명과학연구원&중점연구소) ;
  • 이예준 (경상국립대학교 응용생명과학부(BK21)) ;
  • 김현상 (경상국립대학교 응용생명과학부(BK21)) ;
  • 엄준식 (경상국립대학교 응용생명과학부(BK21)) ;
  • 최유영 (경상국립대학교 응용생명과학부(BK21)) ;
  • 배은지 (국립산림과학원 산림바이오소재연구소) ;
  • 이성실 (경상국립대학교 응용생명과학부(BK21), 농업생명과학연구소 & 중점연구소)
  • Received : 2021.01.27
  • Accepted : 2021.03.23
  • Published : 2021.05.31
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Abstract

The current study was to evaluate the antioxidant activity of Phyllostachys bambusoides (PHB) as a feed additives and investigate whether its antioxidant activity could be helpful for increasing rumen fermentation characteristics and methane reduction. The antioxidant activity results showed that total polyphenols and flavonoids contents were 43.54 ± 8.68 mg CE/g and 17.13 ± 0.45 mg QE/g, respectively, and the IC50 values for 1,1-diphenyl-2-prcrylhydrazyl (DPPH) and 2,2'-azino-bis (3- ethylbenzthiazoline-6- sulphonic acid) (ABTS) radical scavenging activity were 163.13 ± 19.25 ㎍/mL and 97.07 ± 4.46 ㎍/mL, respectively. Two heads of cannulated Hanwoo (450 ± 30 kg), consuming timothy hay and a commercial concentrate (60:40, w/w) twice daily (at 09:00 and 17:30) at 2% of body weight, with free access to water and a mineral block, were used as rumen fluid donors. An in vitro incubation experiment was performed after 6, 12, 24, 48, and 72 hr with PHB added at concentration of 2, 4, and 6% of timothy hay basis. Total gas emission decreased as the amount of PHB addition increased at 6 and 24 hr of incubation. However, PHB addition did not affect total volatile fatty acid production, and methane and carbon dioxide emission also decreased as the amount of addition increased at 48 hr of incubation. Therefore, PHB was expected to be used as methane reducing additives in the ruminants.

이번 연구는 반추동물의 메탄 감소에 효과가 있는 천연 사료첨가제를 찾고자 실시하였다. 왕대의 총 폴리페놀 및 플라보노이드 함량은 각각 43.54 mg CE/g 및 17.13 mg QE/g이였으며, DPPH 및 ABTS 유리기 소거 활성은 IC50 값이 각각 163.13 및 97.07 ㎍/mL으로 측정되었다. In vitro 시험은 왕대의 첨가수준을 달리하여 발효 시간대별(6, 12, 24 및 48 hr) 실시하였다. pH는 발효시간동안 적정 범위였으며, 건물 소화율 및 미생물 성장량은 모든 발효 시간대별 처리구에서 대조구와 유의적(P>0.05)인 차이를 보이지 않았다. 총 가스 발생량은 발효 6 및 24시간에 첨가량이 증가함에 따라 감소(Linear effects, 6 hr, P=0.0004; 12 hr, P=0.0055)하였다. 메탄 및 이산화탄소 발생량은 발효 48시간대 첨가량이 증가함에 따라 감소하였다(Linear effects, P=0.022; P=0.0188). 또한 왕대의 첨가는 총 휘발성 지방산 생성량에 부정적인 영향을 미치지 않았고, 발효 48시간대 butyrate 함량은 첨가량이 증가함에 따라 증가(Linear effects, P=0.0001)하였다. 따라서 플라보노이드를 함유하고 있는 왕대의 첨가는 in vitro 반추위 메탄을 저감할 수 있고 발효성상에 부정적인 영향을 미치지 않았다. 그러므로 반추동물 메탄저감 첨가제로서의 활용 가능성이 있을 것으로 기대된다.

Keywords

Acknowledgement

본 연구는 농촌진흥청 연구사업(세부과제번호: PJ01477803)에서 지원 받았음.

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