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Screening the level of cyanogenic glucosides (dhurrin) in sorghum accessions using HPLC analysis

  • Choi, Sang Chul;Chung, Yong Suk;Lee, Yun Gyeong;Park, Yun Ji;Kim, Changsoo
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.104-104
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    • 2017
  • Sorghum (Sorghum bicolor (L.) Moench.) is one of the most important crops for human and animal nutrition. Nonetheless, sorghum has a cyanogenic glucoside compound which can be degraded into hydrogen cyanide, toxic to humans and animals even with tiny amount. In consequence, breeding materials with a low cyanide level has been a top priority in sorghum breeding programs. To fulfill our long-term goal, we are screening sorghum accessions with low cyanide level, which would be an important breeding material for food safety. We collected seeds of various sorghum accessions and analyzed relevant metabolites to find useful breeding materials of sorghum accessions containing low cyanide. Fourteen wild relatives were obtained from the University of Georgia in US, a reference accession BTx623, and three local varieties from National Agrobiodiversity Center of Rural Development Administration in Korea, and one wild species from the Wild Plant Resources Seed Bank of Korea University in Korea. Sorghum plants were grown in plastic greenhouse under natural conditions. After growing, leaf samples were harvested at different developmental stages: seedling phase, vegetative phase (right before flowering), and reproductive phase (ripening). Using collected samples, quantification analysis were performed by an HPLC system for three metabolites (dhurrin, 4-hydroxybenzaldehyde, and 4-hydroxyphenylacetic acid) in sorghum plants. Prior to metabolome analysis, specific experimental condition for HPLC system was set to be able to separate three metabolites simultaneously. Under this condition, these metabolites were quantified in each accession by HPLC system. We observed that the metabolite contents were changed differently by developmental stages and accessions. We clustered these results into five groups as patterns of their contents by developmental stages. Most of accessions showed that 4-hydroxybenzaldehyde content was very high at seedling stage and decreased rapidly at vegetative phase. Interestingly, the patterns of dhurrin content were very different among clusters. However, 4-hydroxyphenylacetic acid content was maintained at low levels by developmental stages in most accessions. The results would demonstrate how dhurrin and alternative degradation pathways are differentiated in each accession.

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A study on the status of recognition, understanding of the use and practical application of oral hygiene devices in dental clinics patients (치과의원 환자의 구강위생용품에 대한 인지도, 용도이해 및 사용실태에 관한 연구)

  • Park, Chung-Soon;Kim, Young-Im;Jang, Sun-Hee
    • Journal of Korean society of Dental Hygiene
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    • v.9 no.4
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    • pp.685-698
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    • 2009
  • Objectives : This study was to examine the recognition and understanding of the use, application of oral hygiene supplies among dental clinic patients in a bid to stress the necessity of education on the right awareness and use of oral hygiene supplies, to provide information on the development of educational programs and ultimately to help promote the oral health of people in general. Methods : The subjects in this study were 314 patients who visited dental clinics in North Jeolla Province in June 2009. Results : 1. Out of oral hygiene supplies, the largest number of the patients investigated(74.6%) were best cognizant of dental floss, and the greatest group(77.7%) had the right understanding of the use of toothpick. Currently, the oral hygiene supplies that were most widely in use were toothpicks(43.2%). 2. As to the relationship of awareness and understanding of the use, application of oral hygiene supplies to subjective oral health status, 50.0 percent of the patients who understood the use of toothpick found themselves to be in good health, and the gaps between them and the others were statistically significant. Among those who were aware of dental floss, the largest group(51.4%) considered themselves to be in good shape, and in the event of those who understood the use of dental floss, the greatest group(49.2%) deemed themselves to be in good health. Out of those who understood the use of mouse rinse, the largest group(53.7%) thought they were in good health(p<0.05). 3. Recognition of interdental brush, understanding of its use and whether to use it currently or not were identified as parents to use oral hygiene supplies recommended by dental clinics(p<0.05). 4. Recognition of interdental brush, understanding of its use and whether to use it currently or not were identified as patients to use oral hygiene supplies recommended by dental clinics(p<0.05). 5. The largest group of those who didn't put dental floss to use didn't use it for other reasons unspecified in the questionnaire, and the second greatest group of them didn't use it since it was so onerous to do that. There were statistically significant differences in the reason why they didn't use the oral hygiene supplies(p<0.05). 6. Awareness of dental floss and interdental brush, understanding of the use of the two and whether to use the two at present or not made statistically significant differences to whether they were likely to use the oral hygiene supplies in the future. And whether they were likely to use the oral hygiene supplies in the future was statistically significantly different according to awareness of mouse rinse and understanding of the use of it as well(p<0.05). Conclusions : Dental hygienists have to provide patients with various data of oral hygiene devices through oral health education and then only patients caring in dental clinics can choose the appropriate devices to claim for their own disease.

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Relationship of health-promotion life style to the factors of oral-health practice among workers (근로자들의 건강증진 생활양식에 따른 구강건강 실천요인)

  • Song, Ji-Yeon;Park, Ji-Young;Kim, Nam-Song
    • Journal of Korean society of Dental Hygiene
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    • v.12 no.3
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    • pp.621-629
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    • 2012
  • Objectives : The purpose of this study was to examine the influential factors for the oral-health practice of adult workers and the relationship of their health-promotion life style to the factors of oral-health practice in an attempt to determine the impact of health-promotion life style. Methods : The subjects in this study were 160 workers who worked in Seoul, North-Jeolla and South-Jeolla Province, and were at the age of 20 and up. The survey was conducted from February 1 to July 20, 2011. Results : As for the relationship of the general characteristics of the workers to the factors of their health-promotion practice, gender made statistically significant differences to toothbrushing female (3.24), and there were statistically significant differences in education/concern according to more than college(2.72) scored higher academic credential(p<0.05). The group of the workers whose health-promotion life style was better in terms of all the toothbrushing(3.35), use of oral hygiene supplies(2.19), regular dental-clinic visit(2.70), dietary control(2.84) and education/concern(3.20) scored higher, and there were statistically significant differences in the factors of education/concern(p<0.05). Their health-promotion life style had an impact on regular dental-clinic visit among the factors of oral-health practice(p<0.05), and there was a positive correlation between health-promotion life style and tooth brushing, dietary control(p<0.05), regular dental-clinic visit, education and concern(p<0.01) the factors of oral-health practice. Conclusions : Workers should be stimulated to get into sound life habits to change their behavior, and they should be urged to improve their preventive health care, to get a dental checkup and ultimately to promote their health as well. Companies should take measures to accelerate the oral health promotion of workers and provide more dental checkup programs for them to be more concerned about their oral health, to promote their oral health and to maintain the best oral health.

Estimation for CDM of Power Generation by using Bio-diesel (바이오 디젤의 발전용 연료화 CDM 평가)

  • Hur, Kwang-Beom;Park, Jung-Keuk;Rhim, Sang-Kyu;Lee, Jung-Bin
    • Journal of Energy Engineering
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    • v.18 no.2
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    • pp.132-135
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    • 2009
  • Development of biofuels like ethanol and biodiesel for commercial uses is a recent phenomenon. However, the growth of ethanol and biodiesel has been impressive during the period 2000-2007yr. Globally, production of biodiesel stands around 8.3 billion liters. Europe leads the world in biodiesel production with 80% share of the global biodiesel production total. Today biodiesel fuels have been in commercial use in many countries and recently the world-wide biodiesel market has experienced considerable growth, which is partly due to various tax concession programs and other financial incentives. In Korea, biodiesel has already been used for transportation fuel, but not used for power generation fuel yet. Korean government has a strategy for renewable energy propagation, especially the goal of power generation amount by renewable energy is 3% of total power production by 2012. This paper focuses on the estimation study for effect of using biodiesel as power generation fuel. The study also has the plan to replace the fuel of thermal power plant, gas turbine and distributed power generation system. As the increase of biodiesel fuel, I look forward to environment-friendly power generation and the strategy of Renewable Portfolio Standards(RPS).

Research of Change of Heart Rate Variability by Psychological Types before and after Meditation Program (α Version) (명상프로그램(α version) 시행 전 후의 심리유형별 HRV 변화 연구)

  • Kim, Geun-Woo;Bae, Hyo-Sang;Kim, Ji-Hwan;Kim, Byoung-Soo;Lee, Pil-Won;Park, Seong-Sik
    • Journal of Oriental Neuropsychiatry
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    • v.26 no.2
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    • pp.89-102
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    • 2015
  • Objectives: To examine the medical effectiveness of meditation programs ($\alpha$ version) by psychological types. Methods: MBTI, which was created by Katharine Cook Briggs and Isabel Briggs Myers, was used as the Psychological Type test and to investigate psychological temperament and functions and psychological preferences. Heart rate variability was used to test the effectiveness of meditation by investigating time domains (mean HR, SDNN, PSI) and frequence domain parameters (TP, LF, HF, LF or HF norm, Ln (TP or VLF or LF or HF). Results: 1. The autonomic nervous system became active, and both time domains and frequency domains showed positive responses to meditation in heart rate variability tests, without distinction of Psychological Types. 2. In Psychological Types using sensing over intuition for perception, there were positive responses as well as an increase of the parasympathetic nervous system's activeness to meditation for heart rate variability tests, depending on psychological temperaments and psychological functions. 3. In heart rate variability tests by preferences, there was no difference. Extroversion and Introversion types, Sensing over Intuition Types, Thinking over Feeling Types, Judging over Perception Types had an increase of activeness of the parasympathetic nervous system. Therefore, meditation has a positive physical and psychological relaxing effect. Conclusions: A complex meditation program has a positive effect on overall meditation. Especially in the MBTI test, sensing was superior to intuition when people recognized objects. The Sensing, Thinking and Judging type was more advantageous than Intuition, Feeling and Perception, respectively. In the future, a well-designed control study is needed, to develop a suitable meditation for each personality type.

Effect of Allergy Related Disease on Suicide Ideation among Adolescents in Korea (청소년 알레르기성 질환의 복합성과 중증도가 자살 생각에 미치는 영향)

  • Wang, Jin Woo;Kim, Eun Young;Park, Su Jin;Lee, Jun Hyup;Rhim, Kook Hwan
    • The Journal of Korean Society for School & Community Health Education
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    • v.17 no.3
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    • pp.11-25
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    • 2016
  • Background & Objectives: There were increasing evidence about the relationship between allergy related disease such as asthma, atopic dermatitis and allergic rhinitis and suicide ideation. However little was known about the concrete relatedness between severity and comorbidity of allergy related disease with suicide ideation. The objective of this study was to investigate the cases of the prevalence of suicide ideation among adolescents with allergy related disease such as asthma, atopic dermatitis and allergic rhinitis, and examine the association between allergy related disease and suicidal ideation among adolescents in South Korea. Methods: Data was based on Korean Youth Risk Behavior Web-based Survey(2014) which was a cross-sectional study containing 34,874 Korean middle and high school students who diagnosed with allergy related disease. We used the weights, strata and primary sampling unit information provided by the public use dataset to compute descriptive statistics and logistic regressions. Computations were done with SPSS version 20.0. Results: 19.9%, 15.6%, 13.8% of adolescents who suffered from one, two and three of allergy related diseases respectively reported having been thought of suicide ideation. Socio-demographic factors were adjusted as control variables. Students with greater severity of disease were more likely to have suicide ideation. Odds ratio for students who were absent one to three days from school because of allergies was 1.96(95% CI 1.51-2.46), and odds ratio for those who were absent more than four days from school was 3.60(95% CI 2.46-5.28). Conclusions: Given that adolescents' severity and comorbidity of allergy related disease were clearly associated with suicide ideation, suicide prevention programs for adolescents with allergy related disease should be improved by strategic approaches towards the severity and comorbidity of disease.

A Study of Disabled Women's Job Needs (여성장애인의 취업 욕구 수준에 영향을 미치는 요인에 대한 분석)

  • Kim, Ki-Tae;Park, Byung-Hyun;Kang, Chul-Hee;Lee, Sung-Kyu;Lee, Kyung-Hee
    • Korean Journal of Social Welfare
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    • v.37
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    • pp.33-66
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    • 1999
  • The primary objectives of this research is to identify (1) demographic characteristics and job needs of women with disabilities in Korea and (2) factors that predicts the degrees of their job needs. This study uses the data based on interviews with 805 disabled women selected randomly from the registered disabled women in Seoul, Kuyngki-Do, Pusan, and Kuyngsangnam-Do. In the aspect of disabilities, this study showed that more than half of disabled women in our sample have very severe disabilities (1st degree and 2nd degree of disabilities) and needs others' help in their daily lives. In the demographic aspect, this study found that disabled women's education level is very low and their monthly income is also very low. This study also found that in spite of their disabilities and functional limitations, more than 3/4 of disabled women have needs about jobs. Finally, using logistic regression analysis, this study found that disabled women's type of disabilities, dependencies in daily life, age, job experiences, job training experiences, willingness for independence, and parental attitude about disabled women's future are statistically significant in predicting the degree of their job needs. This study will contribute to developing knowledge about disabled women's characteristics and provide practical implications for intervention strategies and assistance programs related to promoting their welfare.

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A Study on the Periodic Medical Examination, and Health Care Management Programs in Industries -Kwang-Ju city and Chonnam Province- (산업장의 특성별 건강검진과 보건관리 -광주.전남지역-)

  • Kang, Heoi-Young;Park, In-Hee;Choi, Young-Ae;Oh, Mi-Sung;Choi, Hee-Jung;You, Su-Ok
    • Research in Community and Public Health Nursing
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    • v.4 no.1
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    • pp.58-66
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    • 1993
  • The purpose of this study was to explore the condition of periodic medical examination, and health care services in industries. This will offer some basic data in developing industrial nursing care requirements. To achieve this goal a self-administered questionnaire (developed by the academic affairs of community health nursing) was provided to the nurses working in 40 industries from Dec 20, 1992 to Jan 20, 1993. The statistical computer package SAS, along with t-test, and ANOVA was used to manipulate the date. The results were as follows : 1. General Characteristics: The greater part of the industries studied were manufacturing company, with over 500 employees. The shift system was used with most companies using one or three shifts, and 75.0% of them were organized with Industrial Safety and Health Committees. 2. Periodic Medical Examination : Most of the workers were receiving periodic medical examination from a designated hospital (96.7%). Of those 15.8% had a close medical examination. In the medical examination 9.4% were evaluated at 'C' and 3.8 were evaluated at 'D'. About 55.0% of those workers received the result of the periodic medical examination individually(95.0%). 3. Special Medical Examination : The rate of those who were receiving special medical examination was 98.4% and about 11.7% were evaluated at 'C' and 3.9% were evaluated at 'D'. Those who had any health problems (54.2%) were receiving follow-up care, 52.4% of them had medical treatment while working. The health care managers in the company consulted 97.1% of them who had any suspicious signs and symptoms of occupational diseases. 4. Health Care Services : The average score of health care services was 13.8 out of a possible 28 point. The score of health education and health assessment, was lower than medical diagnosis and environmental hygiene. There were no significant differences in health care services according to the size of the industries(F=.95, P=.429). The score of health care services was higher in the worker who had better health and showed significant differences (F=4.50, p=.025).

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The Effectiveness of School Based Short-Term Social Skills Training in Children with Attention-Deficit/Hyperactivity Disorder(ADHD) (ADHD 초등학생을 위한 학교 중심 사회성기술 훈련 프로그램의 효과에 대한 연구)

  • Paek, Myung-Jae;Ahn, Jung-Kwang;Lim, So-Yun;Kim, Yang-Ryul;Park, Min-Hyeon;Kim, Boong-Nyun;Cho, Soo-Churl;Shin, Min-Sup;Kim, Jae-Won;Kim, Hyo-Won
    • Journal of the Korean Academy of Child and Adolescent Psychiatry
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    • v.20 no.2
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    • pp.82-89
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    • 2009
  • Objectives: Children with attention-deficit hyperactivity disorder(ADHD) often have difficulties in social behavior. The aim of this study was to evaluate the effectiveness of a short-term training program for improving social skills, self-perception and attention deficits. Methods: The subjects were nine children diagnosed with ADHD with(or without) other mental disorders using the Diagnostic Interview Schedule for Children(DISC-ADHD) module. Children were given eight sessions of a social skills training program. Parents of children simultaneously participated in their own training which was designed to support their children's generalization of skills. Assessments included child, parent and teacher ratings of social skills, self-perception and attention deficit at baseline and post-treatment. Results: Social skills training led to significant improvements in child-reported measures of self-esteem, in teacher reported measures of social skills, and in parent-reported measures of attention deficit. Conclusion: This study suggests that short-term social skills training programs for children with ADHD may improve their social skills, self-perception and attention deficits.

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The Influence of Mother's Perfectionism and Parenting Beliefs on Preschooler's Socal Competence (어머니의 완벽주의와 양육신념이 유아의 사회적 능력에 미치는 영향)

  • Park, Young Yae;Kim, Lee Jin
    • Korean Journal of Childcare and Education
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    • v.10 no.1
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    • pp.183-199
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    • 2014
  • The objective of this study was to examine the effects of mother's perfectionism and parenting beliefs on her preschooler's social competence. The participants were 277 mothers residing in Daejeon, Korea. Basic descriptive statistics, Cronbach's ${\alpha}$, t-test, ANOVA, and multiple regression were used for statistical treatment. The results were as follows: First, a mother's perfectionism and parenting beliefs did not differ according to her educational level. One factor of perfectionism, 'holding high standards', alone showed significant difference between highschool graduates and graduate school graduates, the latter's scores being higher than the former's. There were no significant differences in preschooler's social competence by sex or age. However, a few sex and age differences were found in sub-factors of social competence. Girls scored higher than boys on 'showing affection', and 5 year olds scored lower on 'showing affection' and higher on 'leadership' than 3 years olds. Second, a mother's perfectionism and parenting beliefs were able to explain 22.8% of variance in preschooler's social competence, the former showing more predictive power than the latter. Each of the two factors of maternal perfectionism affected five factors of preschooler's social competence in a different manner. 'Holding high standards' of perfectionism positively influenced preschooler's social competence factors such as 'social capability', 'leadership', and 'showing affection', whereas maternal 'fear of failure' had a negative impact on 'showing affection', 'disturbing'(reversed), and 'instability'(reversed). These results were discussed in relation with changes in social atmosphere and value systems, changes in child-rearing behaviors, or the construct and concept of perfectionism itself, It was suggested that these results be utilized in developing parent education programs for preschoolers lacking social competence.