• Microbiology and Biotechnology Letters
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• v.30 no.4
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• pp.298-304
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• 2002

A family shuffling associating PCR-based and in vitro recombination and expression in Escherichia coli cdd mutant was carried out. Two cdd genes encoding cytidine deaminases (CDase) from thermophilic Bacillus caldolyticus and B. stearothermophilus were shuffled. Around 150 viable mutant colonies screened on AB minimal medium without uracil by E. coli cdd complementation were selected for cytidine deaminase assay and 4 candidates (SH1067, SH1077, SH1086, and SH1118) were chosen for the detailed study. The nucleotide sequence analyses of 4 selected mutants revealed that they have several point mutations and recombinations. Surprisingly, the SH 1067 showed 770 fold more specific CDase activity at $80^{\circ}C$ than that of T101 from parental B. stearothermophilus.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.sup1
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• pp.43-47
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• 2016

Second-hand tobacco smoke (SHS) exposure at home, especially among children, is a serious issue in Viet Nam. During the past decade, much effort has been taken for tobacco control in the country, including various prgorammes aiming to reduce SHS exposure among adults and children. This article analysed trends and factors associated with SHS exposure at home among school children aged 13-15 in Viet Nam, using the Global Youth Tobacco Surveys conducted in 2007 and 2014. Descriptive and inferential statistical methods with logistic regression were applied. Overall, there was a significant reduction in the level of exposure, from 58.5% (95%CI: 57.6-59.3) in 2007 to 47.1% (95%CI: 45.4-48.8) in 2014. Of the associated factors, having one or both parents smoking was significantly associated with the highest odds of SHS exposure at home (OR=5.0; 95%CI: 4.2-6.1). Conversely, having a mother with a college or higher education level was found to be a protective factor (OR=0.5; 95%CI: 0.3-0.8).

• Journal of dental hygiene science
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• v.20 no.3
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• pp.126-135
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• 2020

Background: The purpose of this study is to investigate the effects of entrance exam stress on oral health behaviors and subjective oral health status in female high school students. Methods: A self-reported questionnaire was administered to 216 female high school students in the Gwangju area. We performed an independent t-test, one-way analysis of variance (ANOVA), Pearson's correlation coefficient, and multiple regression analysis using the SPSS/WIN 21.0 program. Results: The average of the entrance exam stress was 2.73, and among the sub-areas, the tension for exam/poor stress was the highest with 3.21. As a result of analysis of general characteristics and subjective oral health status, oral mucosal disease was lower in family income level (p<0.05), bad breath was significantly higher with lower academic performance (p<0.001) and family income level (p<0.05). As a result of oral health behavior and subjective oral health status analysis, dental caries has more than 4 times of caries snacks (per week) (p<0.05), bad breath was found to be more perceived when the average number of daily brushing was 2 or less (p<0.01). Factors affecting subjective oral health status were that dental caries was a patients pressure stress (β=0.202); temporomandibular disorder (β=0.227), xerostomia (β=0.342), and oral mucosal disease (β=0.190) were insufficient spare time; bad breath was academic performance (lower) (β=0.231) and insufficient spare time (β=0.184). There was a statistically significant positive correlation between the subjective oral health status and the subarea of entrance exam stress, excluding future uncertainty stress. Conclusion: It is considered that oral health education should be conducted to prevent oral diseases for students with high parental pressure and insufficient spare time stress as well as finding practical ways to reduce entrance stress.

• Human Ecology Research
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• v.53 no.2
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• pp.169-177
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• 2015

The PRQ instrument is parental perspectives of the parent-child relationship. It measured parent-child dimensions that include attachment and involvement. Also, it provides additional information on parenting style, parenting stress and satisfaction with the child's school. This study tested reliability and validity of the Korean version of Parenting Relationship Questionnaire-Child (K-PRQ-C). The K-PRQ-C was administered to parents of 713 elementary school children including 362 boys and 351 girls. They were from 1st to 6th grade children who were recruited form 4 elementary schools in Korea. Reliability was tested through internal consistency. We conducted confirmatory factor analysis. Moreover, scale inter-correlation was conducted for validity test. Discriminant validity conducted through administration to the parents of clinical children with emotional and behavior disorders. Construct validity was also examined through Korean version of the Parenting Sense of Competence. The results of confirmatory factor analysis indicated that the similar factor structures exist for PRQ-C and K-PRQ-C. The results showed a high level of internal consistency as well as construct and discriminant validity. These results showed valid psychometric properties for the K-PRQ-C. The K-PRQ-C provides a reliable and valid means of gathering information about the parent-child relationship. We discussed Implications for the use of the K-PRQ-C as a clinical tool and as a research instrument.

• Child Health Nursing Research
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• v.21 no.2
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• pp.123-130
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• 2015

Purpose: The purpose of this study was to understand the intellectual and psychological features of children with Moyamoya disease who were patients in the Department of Pediatric Neurosurgery of Severance Hospital. Methods: From December 2010 through December 2012, 63 patients with diagnoses of Moyamoya disease and 59 children in a normal group were enrolled. This study was conducted using the Korean-Wechsler Intelligence Scale for Children, Rey-Kim Memory Test for children, Korean Child Behavior Checklist and Pediatric Quality of Life Inventory$^{TM}$4.0. Results: The results showed that the intellectual and psychological profiles of children with Moyamoya disease were lower than the average of the normal control group. The tested patients showed significantly lower scores for Performance Intelligence Quotient cognition level. Also, in terms of quality of life, children with Moyamoya disease had lower levels of physical and school functionality. The results were in line with those of previous studies involving psychological tests of children with chronic diseases. Conclusion: Considering the intellectual and psychological characteristics of children with Moyamoya disease, integrated psychological intervention plans including elements such as supportive therapy for patients and programs for parental education are required.

• Journal of Gifted/Talented Education
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• v.17 no.2
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• pp.251-279
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• 2007

The purpose of this study was to develop a comprehensive model to identify underachieved gifted children in elementary schools by utilizing multiple methods. From March 1st, 2006 through September 30th, 2006, we developed and applied a 6-step comprehensive identification model for underachieved gifted children by examining 1980 children attending S elementary school located in Daegu Metropolitan City, starting from the first step of teacher nomination to the last step of committee's final decision. As a result, 4 students were identified as underachieved gifted children. For comprehensive and appropriate identification, we adopted, adjusted, or developed several standardized tests, teacher nomination form, parental questionnaire, self-report checklist, and school achievement tests, which were fine-tuned in the application process of this identification model. Based on this study, we suggest that a comprehensive identification model for underachieved gifted children in public elementary schools consist of 6 steps: a) teacher nomination, b) simplified standardized testing, c) student's self-report, d) multiple standardized tests, e) qualitative analysis, and f) a committee decision for the final identification.

• Korean Journal of Childcare and Education
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• v.6 no.1
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• pp.1-21
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• 2010

This study examined Korean immigrant elderly's generativity and care-giving experiences of their grandchildren according to their level of generativity. In-depth interview and participant observation method were used. First, this study used Loyola Generativity Scale in order to find Korean immigrant elderly's generativity level. 4 grandparents were selected as a group of low generativity level and 4 grandparents as high level. Grandparents with low generativity level showed non-voluntary care-giving, severe care-giving stress, guilty feeling for their adult children and shame for their ancestors, anxiety for the unstable future, lack of self-care, and lack of social assistance. Grandparents with high generativity level showed voluntary care-giving, positive reaction for the reversed parental care, feeling of usefulness for care-giving, hope for the future, existence of self-care, and existence of social assistance. Further discussion and suggestions were made from the study results.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• v.20 no.2
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• pp.76-81
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• 2009

Ovjectives: As the attention devoted to children's mental health increases, medical costs and burdens mount as well. In the present study, we evaluated the association between socioeconomic status(SES) and major child psychiatric symptoms. Methods: The subjects of this study were children of recruited from a mental health screening program in the Seoul Sungbuk mental health center over the course of 3 months. To establish the SES of each child, we collected data about each child's medical insurance, years of parental education, household income, family structure and housing. 149 children & parents completed questionnaires including the Childhood Depression Inventory(CDI) and the Korean Attention Deficit Hyperactivity Disorder Rating Scale(K-ARS). Results: The mean K-ARS-P score was $12.1{\pm}11.1$ and the suspected prevalence of ADHD was 20.8%(n=31). The mean cm score was $12.9{\pm}7.9$, and the prevalence of suspected depression was 16.8%(25). Depressive symptoms and ADHD symptoms were both more severe than those observed in a previous epidemiologic study in Korea. Depressive symptoms were more closely associated with family SES status. Conclusion: SES status is one of the most important risk factors in the development of major child psychiatric symptoms. In our study we found that depressive symptoms in particular were most tightly associated with psychosocial factors. Evaluation of the risk factors, early screening and intervention for low SES children would be valuable mental health management tactics to implement in a community mental health system.

• Korean Journal of Childcare and Education
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• v.7 no.3
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• pp.101-124
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• 2011

The purpose of this study was to examine the differences of children's basic habits formation by their mother's and father's parenting style and their recognition about the importance of basic habits. The participants of this study were 160 three and four-year-old children's parents. The results of this study are as follows. The parental features on children's basic habits formation were significant different by children's sex. Girls' basic habits were significant different by their mother's and father's recognition about the importance of basic habits. On the other hand, boy's basic habits were significant different by mother's and father's affectionate parenting and their recognition about the importance of basic habits. Especially, boys whose parents did not consider important their children's basic habits formed higher level of basic habits in case that their mothers showed affectionate parenting. Also, there are no significant differences in children's basic habits by autonomous parenting.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.4
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• pp.1841-1848
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• 2013

This study is designed to figure out the smoking status of college students. The main object of the study is to analyze the details of smoking status, the relationship between their allowance and smoking habit and the effect of their parent's smoking. This research was conducted toward 854 of college student(460 males/394 females) from 22 to 29 October 2012. According to the research, 49.3% of the males and 8.93% of females were smokers and the smoking group was affected by their allowance and home environment(parental smoking habits). Compared with existing related research results, this study shows the continuous increase rate of female smoking. Therefore the publicity and education to college students for smoking prevention and cessation measures are required.