• 제목/요약/키워드: Paralytic shellfish toxin

검색결과 44건 처리시간 0.03초

Studies for Reestablishment of Approval Toxin Amount in Paralytic Shellfish Poison-Infested Shellfish -4. Detoxification and Toxin Composition in Paralytic Shellfish Poison-Infested Oyster during Processing-

  • Jeong Hyun-Jeong;Shin Il-Shik;Kim Young-Man
    • Fisheries and Aquatic Sciences
    • /
    • 제2권2호
    • /
    • pp.155-160
    • /
    • 1999
  • Studies on detoxification of Paralytic Shellfish Poison (PSP)-infested oyster, Crassostrea gigas were carried out using available processing resources. Changes of paralytic shellfish toxin components and specific toxicity during canning process were also investigated with high performance liquid chromatography (HPLC). Toxic oysters collected at Hachong in Koje Bay were used for experimental samples. The toxicity of oysters with range of 185-778 ug/100g was reduced below the quarantine limit of 80 ug/100g or not detected level by the mouse bioassay after canning process. The mole $\%$ of toxin components in the shucked oyster was in the order of 25.1 mole $\%$ of gonyautoxin 1, 19.2 mole $\%$ of gonyautoxin 3, 17.2 mole $\%$ of gonyautoxin 4 and 14.6 mole $\%$ of gonyautoxin 2. This sample had tracing amounts of Cl, C2, saxitoxin and neosaxitoxin. In the case of specific toxicity, the major toxins were consisted of gonyautoxin 1-4. The sum of gonyautoxin 1, 2, 3 and 4 was 80% of total toxicity of oyster. Saxitoxin and decarbamoylsaxitoxin were the more thermostable than any other toxin components.

  • PDF

Studies for Reestabilishment of Approval Toxin Amount in Paralytic Shellfish Poison-Infested Shellfish 2. Change of Toxin Composition and Specific Toxicity in Paralytic Shellfish Toxins of Blue mussel, Mytilus edulis and, Oyster, Crassostrea gigas from Woepori, $K\v{o}je$, Korea During Canning Process

  • SHIN Il-Shik;CHOI Su-Ho;LEE Tae-Sik;LEE Hi-Jung;KIM Ji-Hoe;LEE Jong-Soo;KIM Young-Man
    • 한국수산과학회지
    • /
    • 제29권6호
    • /
    • pp.900-908
    • /
    • 1996
  • Changes of paralytic shellfish toxin components and specific toxicity in blue mussel, Mytilus edu/is and oyster, Crassostrea gigas during canning process were investigated by high performance liquid chromatography (HPLC). The $mole\%$ of the frozen shucked blue mussel were in order of $27.5\;mole\%$ of gonyautoxin 1, $23.0\;mole\%$ of gonyautoxin 8 (C1) and $23.0\;mole\%$ of epi-gonyautoxin 8 (C2), while those of the frozen shucked oyster were in order of $29\;mole\%$ of C1, $22\;mole\%$ of C2, $16.7\;mole\%$ of gonyautoxin 2. Both samples had minor amounts of saxitoxin and neosaxitoxin. On the other hand, in case of specific toxicity, the major toxins were consisted of gonyautoxin $1\~4$ in both sample. The toxicity of gonyautoxin $1\~4$ were 88 and $84\%$ in blue mussel and oyster, respectively. According to the experimental results, C1, C2 and gonyautoxin 4 were very sensitive to heat treatment, while gonyautoxin 2 and saxitoxin were pretty heat resistant than any other toxin components.

  • PDF

Occurrence of Toxic Alexandrium and Intoxification of Two Mollusk Species by Paralytic Shellfish Poisoning Toxins on the Southeastern Coast of Korea

  • Kim Young-Soo;Lee Jong-Soo;Jang Joon-Ho;Kim Keun-Yong;Kim Chang-Hoon
    • Fisheries and Aquatic Sciences
    • /
    • 제8권2호
    • /
    • pp.76-82
    • /
    • 2005
  • We analyzed the paralytic shellfish poisoning (PSP) toxins of the toxic marine dinoflagellate Alexandrium tamarense collected from Dadaepo and Gaduck-do in Busan and from Sujeong-ri in Jinhae Bay, Korea, in April 2003. We also analyzed the PSP toxin of mussels (Mytilus galloprovincialis) and oysters (Crassostrea gigas) collected around Busan and Jinhae Bay. PSP toxin analyses were conducted by high performance liquid chromatography (HPLC). Fifteen cultured A. tamarense isolates contained 2.78 to 57.47 fmol/cell, with nearly identical toxin profiles: major components C2, GTX4; minor components C1, GTX1, NEO; and trace components GTX2, GTX3, STX. PSP toxin contents were 0 to $492\;\mu{g}$ STXeq/100 g in mussels and 0 to $48\;\mu{g}$ STXeq/100 g in oysters. Mussels at Gijang and Sujeong-ri contained the most PSP toxin contents ($492\;\mu{g}\;STXeq/100\;g\;and\;252\;\mu{g}\;STXeq/100\;g,\;respectively$), exceeding the quarantine level ($80\;\mu{g}$ STXeq/100 g). Their dominant toxin components were C2, C1, GTX2, and GTX3; the minor components GTX1, GTX4, GTX5, and NEO were sporadically detected. Phytoplankton contained 0.774 fmol/L seawater and 1.228 fmol/L seawater at Gijang and Sujeong-ri in April. At that time, Alexandrium cells were present in the water column at Gijang at 2,577 cells/mL and at Sujeong-ri at 6,750 cells/mL. Overall, we found the high and similar PSP toxin contents in AZexandrium isolates and mussels, and a correlation between occurrence of toxic Alexandrium cells in the water column and mussel intoxification. High densities of toxic Alexandrium cells in the water column immediately preceded shellfish intoxification at Gijang and Sujeong-ri in April.

진해만산 와편모조류 Alexandrium속 휴면포자 발아체의 마비성패독 조성 (Paralytic Shellfish Toxin Profiles of the Dinoflagellate Alexandrium Species Isolated from Benthic Cysts in Jinhae Bay, Korea)

  • KIM Chang-Hoon
    • 한국수산과학회지
    • /
    • 제28권3호
    • /
    • pp.364-372
    • /
    • 1995
  • 1993년 진해만 일원의 마비성패독 발생의 원인규명을 위한 모니터링의 일환으로 원인생물의 독생산과 성분조성을 조사하기 위하여 양식장 인근해역의 저서 휴면포자를 발아시켜 분리한 무균주의 독성분을 분석하였다. 분리된 전체주중에서 수정리산 (St. 1) 5주, 욱곡리산 (St. 2) 3주 및 대곡리산 (St. 4) 11주의 독조성 및 독함량을 비교하였을 때, 각 지점별 평균 독량은 약 54-70 fmol/cell 높은 량을 나타내었고, 동일 휴면포자의 clone 분리주 뿐만 아니라 전체 분리주에서 개체별 독함량의 차이가 크게 나타났다. 독조성은 C1/C2 (epiGTX8/GTX8), GTX1/GTX4 및 neoSTX가 주요 구성성분을 이루었고, GTX2/GTX3, GTX5, C4, dcSTX 및 STX성분은 미량 또는 산발적으로 출현하였다. 주요 성분중에서 neoSTX는 $5-54mol\%$로 변동이 컸으나, 전체 분리주의 절반은 출현을 보이지 않아 이 지역에서 조성이 다른 개체군의 출현이 시사되었다 한편, 상대적으로 독성이 강한 GTX1-4 및 neoSTX와 같은 Carbamate군의 성분이 세 정점에서 각각 $57\%,\;54\%$$67\%$의 높은 평균치를 나타내어 이 지역에서의 높은 독화율과 독화 가능성의 잠재력이 큰 것으로 시사되었다.

  • PDF

해양 유독생물의 독성 검사와 보건환경 모니터링을 위한 조건센서의 활용 (Practical Use of Tissue Biosensor for Safety Test of Marine Organism and Monitoring of Public Health and Environment)

  • 천병수;유종수;유진형;도변탈생
    • KSBB Journal
    • /
    • 제14권1호
    • /
    • pp.1-8
    • /
    • 1999
  • It confirmed the facilitated diffusion of $Na^+$ of frog bladder membrane which is a tissue membrane. The mechanism was explained in $Na^+$ channel model and its referred to the $Na^+$ channel obstruction ingredient which was contained in the reference to the $Na^+$ channel obstruction ingredient and son on, e.g., seaweed, shellfish, pufferfish, phytoplankton and chinese drug. Also, it introduces the result which studied from the barrier point of the application of the tissue biosensor to the trade friction on Korea or Japan pufferfish and the marine environment in the one with high dependance. It was possible for the poison quantity of small amount pufferfish toxin (TTX), paralytic shellfish poisoning (PSP) to be measured and also to measure poison quantity in the cultivation poisonous toxin phytoplankton individual. In future, as for this tissue biosensor, it expects that it is possible to contribute widely until environment watch and also monitoring to the scene.

  • PDF

1996년과 1997년의 거제연안 자연산 홍합의 독화 양상과 마비성패독 (PSP)의 모니터링 (Monitoring of Intoxication and Toxin Composition on Wild Mussels (Mytilus corsucus) from Coastal Waters near Koje Island, Korea in 1996 and 1997)

  • 전중균;한명수
    • 한국수산과학회지
    • /
    • 제31권6호
    • /
    • pp.817-822
    • /
    • 1998
  • 1996년 2월부터 1997년 2월까지 월 2회씩 경남 거제군 장목만에서 자연산 홍합 (Mytilus corsucus)을 채집하석 PSP 독화상황을 조사하였다. 홍합의 독성은 마우스를 이용한 생물검정법으로 측정하였고, 독조성은 HPLC를 사용하여 분석하였다. 그 결과, 독성은 3월 27일부터 5월 27까지의 시료에서 검출되었으며, 또한 11월 28일의 시료에서도 검출되었다. 본 연구는 국내에서 가을철에도 PSP에 의해 패류가 독화한다는 것을 처음으로 확인하고 보고한 것이다. 한편 독화한 홍합의 독조성은 봄철 시료의 경우에 GTX군이 주성분이고 STX군과 PX군이 부성분이었으나, 가을철 시료는 봄철의 것에 비해 고독성 성분인 GTX군과 STX군이 적고 저독성 성분인 PX군의 조성이 큰 특징을 보였다.

  • PDF

LC-MS/MS를 이용한 패류 및 피낭류 중 마비성 패류독소 분석법의 유효성 검증 (Validation of LC-MS/MS Method for Analysis of Paralytic Shellfish Toxins in Shellfish and Tunicates)

  • 조성래;김동욱;유헌재;조성해;류아라;이가정;목종수
    • 한국수산과학회지
    • /
    • 제53권2호
    • /
    • pp.174-180
    • /
    • 2020
  • The mouse bioassay has been used widely for the monitoring of paralytic shellfish toxins (PSTs) in many countries. However, this method shows low sensitivity and high limit of detection (LOD), as well as it cannot confirm toxic profiles. Recently, LC-MS/MS method was studied for the quantitative of PSTs, however, the method has any problems with unstable retention times by ionization suppression caused by high salt concentration in shellfish extracts. To establish an alternative method for PSTs analysis, we tried to original LC-MS/MS methods adding desalting operation using amorphous graphitized polymer carbon solid-phase extraction cartridges. The method validation was conducted to determine linearity, limit of detection, limit of quantification (LOQ), accuracy, and precision in quantifying PSTs. The correlation coefficients for all tested PSTs maintained over 0.999. The LODs and LOQs for all PSTs were about 0.19-1.05 ㎍/kg and 0.58-3.18 ㎍/kg, respectively. The accuracies for PSTs were 95.4-107.7% for saxitoxin group, 97.1-100.9% for gonyautoxin group, 99.0-100.8% for N-sulfocarbamoyl toxin group, and 96.8-104.6% for decarbamoyl toxin group. These results indicate that the modified LC-MS/MS method was appropriate for analyzing the PSTs in shellfish and tunicates.

Variation and Profile of Paralytic Shellfish Poisoning Toxins in Jinhae Bay, Korea

  • Mok, Jong-Soo;Song, Ki-Cheol;Lee, Ka-Jeong;Kim, Ji-Hoe
    • Fisheries and Aquatic Sciences
    • /
    • 제16권3호
    • /
    • pp.137-142
    • /
    • 2013
  • To understand critical aspects of paralytic shellfish poisoning (PSP) in a chief area of bivalve production in Korea, seasonal variation in PSP toxins in bivalves collected from Jinhae Bay, Korea in 2009 was surveyed by the pre-column high-performance liquid chromatography oxidation method. We also confirmed the profiles of major bivalves such as oysters Crassostrea gigas and mussels Mytilus galloprovincialis in Jinhae Bay. PSP toxins in the bivalves showed remarkable seasonal variation. PSP toxin levels were detected from April to May in 2009, and the highest total toxin levels at all stations were recorded in May. The major toxins in bivalves were gonyautoxin [GTX] 1&4 and C 1&2; in oysters GTX 2&3 were also detected as major components. GTX 1&4, which showed the highest PSP toxin levels at each station, accounted for the highest proportions of toxin components in mussels and oysters (64.5-71.3% and 41.4-42.4%, respectively). It was also confirmed that the highest toxicity (in ${\mu}g$ saxitoxin [STX] eq/g) was derived from GTX 1&4. The highest total toxicity (in ${\mu}g$ STX eq/g) was approximately 2-8-fold higher in mussels than in oysters collected from the same station. PSP toxin levels in bivalves differed significantly according to the sample collection station. However, the profiles of toxins in the bivalves did not show significant differences during the survey period according to sample collection station. This study shows that PSP toxin levels in some samples from Jinhae Bay were above the regulatory limit in Korea during a specific period in spring.

독성 Alexandrium tamarense를 섭취한 담치류 4종의 마비성패독 축적 (Paralytic Shellfish Poisoning Toxin Accumulation in Four Mussel Species Fed on Toxic Alexandrium tamarense)

  • 김영수;손명백;김창훈
    • 한국수산과학회지
    • /
    • 제39권1호
    • /
    • pp.49-54
    • /
    • 2006
  • Cultured cells of the toxic Alexandrium tamarense were fed to four mussel species, Mytilus coruscus, M. edulis, M. galloprovincialis and Septifer vulgatus, to examine the interspecies and interlocality differences in the ability to accumulate paralytic shellfish poisoning (PSP) toxins. Toxin content of A. tamarense cells varied during culture period. In contrast, toxin composition in the cell (C1,2, GTX1-4 and neoSTX) was constantly stable. In feeding experiment, the four mussel species collected from Geoje intoxicated after uptake of A. tamarense. Toxin content ($average{\pm}SD\;{\mu}g$ STXeq/100 g) of M. coruscus, M. edulis, M. galloprovincialis and Septifer vulgatus were $1,660{\pm}79,\;3,914{\pm}2,242,\;5,626{\pm}1,620\;and\;958{\pm}163$, respectively. Toxin profiles included C1,2, GTX1,4 and neoSTX as the major components, and dcGTX2,3, GTX2,3, neoSTX and STX as the minor ones. Toxin accumulation of three mussel species collected from Pohang, Geoje and Anmyon-do showed interspecies and interlocality differences. Toxin content ($average{\pm}SD\;{\mu}g$ STXeq/100 g) were $91{\pm}4,\;151{\pm}14,\;39{\pm}3$ in M coruscus, $189{\pm}1,\;231{\pm}11,\;206{\pm}15$ in M edu/is and $214{\pm}28,\;326{\pm}30,\;291{\pm}26$ in M. galloprovincialis in order of Anmyon-do, Geoje and Pohang.

진해만 해역에서 지중해담치 (Mytilus galloprovincialis)의 마비성패독 독화 양상 (Paralytic Shellfish Poisoning of Mediterranean mussels from Jinhae Bay in Korea)

  • 손명백;김영수;김창훈
    • 한국수산과학회지
    • /
    • 제42권4호
    • /
    • pp.366-372
    • /
    • 2009
  • This study looked at toxicity of Mediterranean mussels, Mytilus galloprovincialis, which had accumulated paralytic shellfish toxins (PST) from early March to late May 2005 at Jinhae Bay, Korea. Alexandrium sp. was observed in low densities (< 1,000 cells/L) at the beginning of the study in March, increased rapidly in April, declined rapidly and disappeared in May. Although low densities of Alexandrium sp. were observed in March, mussel toxicity exceeded regulation level ($80{\mu}g$ STXeq. /100 g). Peak PSP (Paralytic Shellfish Poisoning) toxicity in the mussels occurred during high Alexandrium sp. cell densities in April. Mussels toxicity decreased with decline of Alexandrium sp. cell density. Major toxin components identified were $GTX_1$, $GTX_4$, followed by $C_1$, $C_2$, $GTX_2$, $GTX_3$ and neoSTX. Trace or sporadic toxin components were STX, $GTX_5$, $dcGTX_2$, $dcGTX_3$ and dcSTX. Toxin component analysis from the middle to end of the study showed that $11{\beta}$-epimers ($GTX_{3,4}$, $C_2$) were converted into $11{\alpha}$-epimers ($GTX_{1,2}$, $C_1$) and started to determine STX.