• Applied Microscopy
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• v.28 no.2
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• pp.181-191
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• 1998

The spermatogenesis of pale chub (Zacco platypus) was investigated morphologically. The testis of pale chub contained numerous testicular sacs. These testicular sacs were bounded on neighboring sacs by single layer of squamous cells. Also, differentiated sperms were filled in the sacs. In the stage of spermatogonium, the germ cells had a large nucleus and a distinct nucleolus, and mitochondrial development was prominent. In the primary and secondary spermatogonia, these cells had a round electron-dense nucleus, reduced cytoplasm, and mitochondria were congregated in the side of cytoplasm. The highly condensed chromatin of sperms was electron-dense, the acrosome was not found in the head of the sperm and a motile flagellum consisted of an axoneme with a typical 9+2 pattern of microtubules.

• Environmental Analysis Health and Toxicology
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• v.28
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• pp.16.1-16.8
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• 2013

Objectives Fish vitellogenin (VTG) is produced in the female liver during oogenesis through the estradiol cycle and produced in the male liver by endocrine disrupting chemicals (EDCs) such as alkylphenols. In this study, we propose that the VTG concentration in the pale chub could be detected using monoclonal antibodies and polyclonal antibodies against vitellin (Vn) in a VTG enzyme-linked immunosorbent assay (ELISA) system. Methods Monoclonal antibodies and polyclonal antibodies were produced using the Vn extracted from the matured ovum of the ovary. The VTG was extracted from the plasma of the male pale chub. The Vn and VTG were confirmed by measuring the molecular weight of their proteins using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and the specificity of the antibodies was checked through western blotting methods. The assay system was validated with respect to optimal assay concentrations, specificity, recovery, and intra- and inter-assay variations. Results The Vn consisted of two protein bands with apparent molecular weights of 64 and 37 kDa. The SDS-PAGE indicated protein weights of 146 and 77 kDa in the VTG. The assay range was 15.6 ng/mL to 2,000 ng/mL, and the value of the intra- and inter-assay variations were within 10.0% and 14.7%, respectively. The recovery rate was $99.5{\pm}5.5%$. Conclusions A sandwich ELISA was developed that could be used to qualify the VTG of pale chub in screening for EDCs. Pale chub is an ideal species for observing estrogen activity in the environment because of its extensive habitat and extensive food chain. The ELISA developed here would be more favorable than those for other species for determining the effect of long-term food chain accumulation of EDCs in aquatic environments.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.51 no.1
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• pp.47-53
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• 2018

The presence of pathogens in wild fish is a potential threat to the fish being raised nearby fish farm. Surveillance of these pathogens in the wild is, thus, highly important to keep fish in the farm safe from serious communicable diseases. Fish and crustacean were sampled 4 times at Soyang Lake in 2016. Pathogens for five reportable communicable diseases including KHVD (koi herpesvirus disease), SVC (spring viraemia of carp), EUS (epizootic ulcerative syndrome) and WSD (white spot disease), and parasites were investigated. In Soyang Lake, pale chub Zacco platypus was the most abundant. Forty seven pale chub, 8 Korea piscivorous chub Opsarichthys uncirostris, 3 oily shiner Sarcocheilichthys variegatus wakiyae and 2 crucian carp Cyprinus carassius were investigated for detection of target diseases of fish. Seventeen Caridina denticulata denticulata, 12 Oriental river prawn Macrobrachium nipponense and 1 Chinese mitten crab Eriocheir sinensis were used for detection of WSD. As the result, reportable communicable diseases were not detected in all the freshwater animals. Dactylogyrus was detected in Korea piscivorous chub. Copepoda was also detected in pale chub and Korea piscivorous chub. Metacercaria was detected in the pale chub sampled in September and October. Those were not metacercaria of liver fluke Clonorchis sinensis.

• Journal of Aquaculture
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• v.21 no.4
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• pp.213-217
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• 2008

This study investigated the effect of supplement of the pigment source in diets on body color for round tailed paradise fish Macropodus chinensis and the pale chub Zacco platypus. Fish (the round tailed paradise fish: 1.6 g/ fish, pale chub: 2.4 g/fish) were fed diets containing various pigment sources (Porphyra powder, Spirulina powder. yeast astaxanthin and paprika extract) twice a day for 6 weeks. Survival of all treatments was above 80%. Weight gain and total carotenoid contents of round tailed paradise fish fed the diet containing Porphyra powder was higher than that of fish fed the other diets. The apparent redness of skin and fin in round tailed paradise fish fed the diets containing paprika extract and yeast astaxanthin was better than that of fish fed the diets containing Porphyra powder, Spirulina powder and the control diet. Weight gain and total carotenoid contents of pale chub fed the diet containing paprika extract were higher than that of fish fed the containing Porphyra powder, yeast astaxanthin, Spirulina powder and the control diet. The apparent redness of skin and fin in pale chub fed the diets containing parika extract and yeast astaxanthin was better than that of fish fed the diets containing Porphyra powder, Spirulina and the control diet. The results of this study suggest that dietary supplementation with paprika extract and yeast astaxanthin can improve redness of skin and fin of round tailed paradise fish and pale chub.

• Applied Microscopy
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• v.25 no.3
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• pp.63-74
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• 1995

The development of pale chub oocyte from the immature oogonium to mature oocyte was investigated by light and electron microscope. The cytoplasm of pale chub oogonia was acidic and many vesicles were located at inner side of nuclear membrane. In primary oocytes, yolk vesicles were distributed in cytoplasm. Also, fibrous materials and protuberances were distributed on the surface of zona radiata. The nucleus of secondary oocyte was enlarged and yolk vesicles in cytoplasm migrated to zona radiata. In early egg, yolk mass are formed and yolk vesicles were located at inner side of zona radiata. Three-layered zona radiata was about $3{\mu}m$ in thickness. The three layers were an outer fibrous material layer, a middle nurse cell layer in which microvilli of early egg cytoplasm contact with processes of nurse cells, and an inner layer with high electron density. In mature egg, euchromatin and a germinal vesicle were developed, mitochondria, free ribosomes, and yolk mass were distributed in cytoplasm. But, yolk vesicles were disappeared. Specially, zona radiata of matured eggs were better thin than the one of immature eggs In conclusion, it is summerized that the oogenesis of pale chub were the increase of cell size, the formation and accumulation of yolk, the decrease in nucleat electron density, changes of zona radiata, and the development of microvilli.

• Journal of Korea Water Resources Association
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• v.41 no.4
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• pp.423-432
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• 2008

The local migration or movement behavior of fishes in streams are related to feeding, spawning, growing, dispersing, and refuging. The pale chub (Zacco platypus) is a dominant species that migrates locally and inhabits in river and stream in Korea. However, dams, weirs, culverts and other regulatory structures are physical barriers that limit fish movement and fragment habits and populations. If main stream and off-channel habitats are connected with culverts, they would restrict the small fish as pale chub movement due to the high flow velocities and low depths. But in Korea, there is no experimental study to evaluate the swimming performance of species in Korea. Therefore, it is difficult to proposed that design guidelines for pass fishes through culverts. The purpose of this experimental study is to evaluate the swimming performance of pale chubs. A series of swimming performance test has been used in both of the fixed velocity and the incremental velocity methods in an experimental flume. As a result, the critical swimming speed for pale chub(body length 8.9 cm) was found to be about 0.7 m/s. Therefore, the flow velocity for culvert design in the low flow condition should not be exceed the its swimming ability, especially 0.7 m/s for pale chubs(body length 8.9 cm). And the minimum depth for culvert design in the low flow condition should not be lower than the fish body height add a dorsal fin height.

• Applied Microscopy
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• v.30 no.4
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• pp.321-326
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• 2000

The ultrastructure of the fertilized egg envelope from pale chub , Zacco platypus belong to Cyprinidae was studied using scanning and transmission electron microscopes. The fertilized egg was an adhesive type, have a single micropyle resembling the pathway of sperm in the area of the animal pole. The micropyle was surrounded by 5 peaks of hill. An outer surface of the fertilized egg envelope was arranged by pore canals irregularly. In section of fertilized egg, the egg envelope consistes of three layers, an outer adhesive layer, a middle electron dense layer and an inner lamellae layer consisting of 9 layers. These ultrastructural characters of fertilized egg envelope from pale chub can be utilized in taxonomy of teleost.

• Environmental Analysis Health and Toxicology
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• v.21 no.1 s.52
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• pp.35-43
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• 2006

Reproduction and organ structure of the pale chub, Zacco platypus in Isa stream were investigated by means of histological methods. The results of the study confirmed reproductive abnormality and histopathological features in the pale chub. The gonadosomatic index (GSI) of the fish showed two peak in April and August. In summer season, GSI of the male was about two times of the female's GSI. Monthly variation of the gonadal development was very irregular. From the histological analysis of the organ structure, epidermal atrophy, necrosis and hyperplasia of pigment cell were observed in the skin. Epithelial layer lifting and clubbing of the lamella and bifurcation of the filament were observed in the gill. Also histological changes as congestion, cytoplasmic degeneration of hepatic cell, degeneration of bile duct, glomerular dilatation, degeneration of renal tubule and pycnosis of interstitial cell were identified in the liver and kidney, respectively.

• Korean Journal of Ichthyology
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• v.29 no.3
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• pp.190-196
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• 2017

The egg and larval development of pale chub, Zacco platypus were determined by observation of laboratory reared larvae from hatching. The experimental adult fish were collected in Wicheon of Nakdong river in June 2016, The eggs and larvae were reared in the experimental tanks, and observed their morphological change. The egg diameter ranged from 1.68 to 1.78 mm ($mean{\pm}SD$, $1.73{\pm}0.07mm$, n =30). The eggs were hatch 70 hours after fertilization. The total length (TL) of the newly hatched larvae ranged from 5.39 to 5.42 mm ($5.40{\pm}0.02mm$, n=10). On the 8 days after hatching, 10 fin rays were formed in caudal fin at 7.89~7.93 mm TL ($7.91{\pm}0.02mm$, n=10). On the 44 days after hatching, the juveniles ranged from 16.1~16.5 mm TL ($16.3{\pm}0.28mm$, n=10).

• Applied Microscopy
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• v.42 no.2
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• pp.53-59
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• 2012

We evaluated the level of toxicity by LC50 and investigated the mechanism of brain impairment and GFAP expression by light and fluorescence microscopes in the pale chub, Zacco platypus, treated with fenvalerate. Survival rate was decreased according to the rise of fenvalerate concentration, and LC50 concentration was $27.79{\mu}g/L$. Apoptosis was increased according to the rise of fenvalerate concentration by TUNEL assay which determine apoptotic cell death population. Also, GFAP expression was increased in the periventricular zone. These results suggest that apoptosis might be a major mechanism to brain impairment of the pale chub by fenvalrerate. Increased GFAP expression in the periventricular zone would be an index of brain impairment. Taken together, this study might contribute to reveal the pathological mechanism of fish brain impairment by insecticide of pyrethroid, and to be an useful basic data for preservation of aquatic ecosystem.