Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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Effects of Fenvalerate on Apoptosis Level and GFAP Expression in the Brain Tissue of the Pale Chub (Pisces: Zacco platypus)

Fenvalerate가 피라미(Zacco platypus) 뇌 조직에서 apoptosis 정도와 GFAP 발현에 미치는 영향

  • Kim, Sung-Woo (Department of Life Science, Cheongju University) ;
  • Park, No-Kwan (Department of Life Science, Cheongju University) ;
  • Lee, Sang-Rae (The National Primate Research Center (NPRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Reu, Dong-Suck (Department of Life Science, Cheongju University)
  • 김성우 (청주대학교 이공대학 생명과학과) ;
  • 박노관 (청주대학교 이공대학 생명과학과) ;
  • 이상래 (한국생명공학연구원) ;
  • 류동석 (청주대학교 이공대학 생명과학과)
  • Received : 2012.03.26
  • Accepted : 2012.06.27
  • Published : 2012.06.30
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Abstract

We evaluated the level of toxicity by LC50 and investigated the mechanism of brain impairment and GFAP expression by light and fluorescence microscopes in the pale chub, Zacco platypus, treated with fenvalerate. Survival rate was decreased according to the rise of fenvalerate concentration, and LC50 concentration was $27.79{\mu}g/L$. Apoptosis was increased according to the rise of fenvalerate concentration by TUNEL assay which determine apoptotic cell death population. Also, GFAP expression was increased in the periventricular zone. These results suggest that apoptosis might be a major mechanism to brain impairment of the pale chub by fenvalrerate. Increased GFAP expression in the periventricular zone would be an index of brain impairment. Taken together, this study might contribute to reveal the pathological mechanism of fish brain impairment by insecticide of pyrethroid, and to be an useful basic data for preservation of aquatic ecosystem.

Fenvalerate에 대한 피라미(Zacco platypus)의 독성 수준을 반수 치사량 (LC50)을 이용하여 평가하고, 뇌 손상의 기작과 GFAP의 발현 정도를 광학현미경과 형광현미경을 이용하여 조사하였다. 피라미의 생존율은 fenvalerate의 농도가 높아질수록 낮아졌고 LC50은 $27.79{\mu}g/L$이었다. TUNEL assay결과 fenvalerate의 농도가 높아질수록 뇌 조직에서 세포고사가 증가하였으며, periventricular zone에서 GFAP 발현이 증가하였다. 따라서 세포고사는 fenvalerate에 의한 피라미 뇌 손상의 주요 기작 중 하나로 생각되며, periventricular zone에서 GFAP의 발현증가는 뇌 손상의 지표로 이용될 수 있을 것이다. 이는 pyrethroid계 살충제에 의한 어류의 뇌 손상기작 연구와 수생 생태계 보존을 위한 기초자료로 활용될 수 있을 것으로 사료된다.

Keywords

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