• 한국잠사곤충학회지
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• 제41권3호
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• pp.174-179
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• 1999

The genetic relationships among six Cordyceps spp. were investigated based on internal transcribed spacer sequences of ribosomal DNA .A portion of the these genes was amplified by PCR. Approximately 590 base pairs were successfully amplified, cloned, sequenced, compared. The nucleotide sequence of the six amplified fragments were aligned by the clustal W program. As a result, Cordyceps militaris shared 87, 96, 98, 90 and 97% sequences homology with Paecilomyces japonica, Paecilomyces sp. J300, Paeciomyces farinosa. Paecilomyces sp. J500 and Cordyceps sinensis, respectively. Paecilomyces japonica also shared 87, 88, 92 and 87% sequence similarity with Paecilomyces sp. J300, Paecilomyces farinosa, Paecilomyces sp. J500 and Cordyceps sinensis, repectively.

• 한국식품영양학회지
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• 제21권1호
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• pp.22-27
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• 2008

This study investigated the quality characteristics of the Seolgiddeok samples prepared with various amounts(0, 0.5, 1.0 and 2.0%) of Paecilomyces japonica powder by examining proximate compositions, color, texture and sensory quality. The composition of the Paecilomyces japonica powder was measured as follows: moisture(7.40%), crude protein(55.77%), crude lipid(7.1l%), and crude ash(5.39%). With increasing Paecilomyces japonica powder content, the moisture and crude protein content of the Seolgiddeok increased; however crude fat and ash contents decreased. In terms of color, the lightness of the Seolgiddeok decreased and yellowness and redness increased with increasing amounts of Paecilomyces japonica powder. Among textural characteristices hardness, cohesiveness, and gumminess were decreased, but springiness increased according to the addition level of Paecilomyces japonica powder. For the sensory evaluation, the overall quality of the SP-l.0% group was not significantly different than the control group(SP group). In conclusion, the optimal addition amount of Paecilomyces japonica powder for Seolgiddeok is proposed as 1% of total weight.

• Toxicological Research
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• 제19권3호
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• pp.241-245
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• 2003

The acute toxicity of Paecilomyces sinclairii was tested in beagle dogs. We daily examined clinical signs, body weights, and hematological/biochemical examinations for 14 days after administration of Paecilomyces sinclairii with different dose levels (0, 0.4, 2 and 10 g/kg). There were no clinical signs and no significant changes in hematological and biochemical analysis. These results showed that Paecilomyces sinclairii did not induce any remarkable acute toxic response and the $LD_{50}$ was greater than 10 g/kg in beagle dogs.

• 한국식품영양과학회지
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• 제34권6호
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• pp.821-824
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• 2005

누에 동충하초(Paecilomyces japonica) 섭취 후 공복혈당은 $27.5\%$, 식후혈당은 $35.5\%$로 감소하였으며, 복용 중단 2주 후에도 실험전 혈당 수준과 비교할 때 각각 $18.2\%,\;27.2\%$ 저하된 상태를 유지하고 있었다. 이는 복용할 때 뿐 아니라 복용 후 어느 정도 기간까지는 지속적으로 혈당 강하 효과가 있음을 의미한다. 남자 당뇨병 환자의 공복혈당은 $28.5\%$, 식후혈당은 $33.5\%$ 감소되었고, 여자 환자의 공복혈당은 $26.1\%$, 식후혈당은 $37.3\%$ 감소되었다. 이러한 결과로 볼 때 누에 동충하초는 인슬린비의존형 성인 당뇨병 환자의 혈당을 감소시키는데 유용한 기능성 식품으로 활용될 수 있을 것으로 판단된다.

• 한국식품조리과학회지
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• 제18권3호
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• pp.280-289
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• 2002

The quality characteristics of breads with different concentrations(2%, 4%) of Paecilomyces japonica (J2, J4) and Cordyceps militaris (M2, M4) powder were compared in terms if sensory and mechanical properties. In the sensory test, J2 and M2 groups gained good scores in taste, texture and odor acceptance as control grourp. Paecilomyces japonica-added group showed higher moisture and bigger volume than that of control group, and Cordyceps militaris group showed lower moisture content and decreasing volume. 'L' values of J and M groups was lower than control, and the longer the storage period, the lower the lightness. The more Paecilomyces japonica was added, the higher the 'a' value was, but Cordyceps militaris showed an opposite trend. The hardness of the bread was the lowest in J2 group and the highest in M4 group, and the longer tile storage period. the higher the hardness was (p＜.001). Overall, the addition of 2% Paecilomyces japonica showed a similar tendency with control group, and showed good scores especially in acceptance, moisture and volume. Paecilomyces japonica appeared to be more desirable than Cordyceps militaris.

• 한국식품영양과학회지
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• 제37권5호
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• pp.548-554
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• 2008

본 연구는 $CCI_4$를 투여한 흰쥐에서 인진쑥과 동충하초를 서로 다른 혼합비율로 투여했을 때 간 손상에 대한 생리활성 기능을 조사하기 위해 혈청 및 지질대사에 미치는 영향을 측정하였다. 사염화탄소의 투여는 간독성을 나타내 간의 무게 및 ALT, AST 수준을 증가시켰으며 이는 인진쑥과 동충하초의 투여에 의해 개선되며 인진쑥과 동충하초를 병행 투여 시 더욱 효과적인 것으로 나타났다. 사염화탄소 투여로 인해 증가된 혈청 콜레스테롤과 중성지방은 인진쑥, 동충하초의 투여로 저하되었으며 혈청 중성지방은 인진쑥과 동충하초를 동량으로 투여한 군에서 유의적으로 낮게 나타났고, 혈청 콜레스테롤은 전체 인진쑥, 동충하초 투여군에서 모두 유의적으로 감소되었으며 인진쑥과 동충하초를 병행 투여하는 경우 더 효과적인 것으로 나타났다. 또한 동맥경화지수는 사염화탄소의 투여 시 정상식이군보다 유의적으로 증가되었으나 인진쑥, 동충하초의 투여로 감소되었으며 4% 인진쑥과 2% 동충하초 병행 투여가 동맥경화지수를 낮추는데 가장 효율적인 것으로 사료된다. 사염화탄소를 투여한 흰쥐의 간장 내 총 지질과 총 콜레스테롤 및 중성지방의 함량은 정상군보다 증가하였다. 인진쑥, 동충하초의 투여는 사염화 탄소 투여로 높아진 중성지방과 콜레스테롤을 감소하는 효과를 보였으며 4% 인진쑥과 2% 동충하초를 병행 투여 시가장 효과적인 것으로 나타났다. 본 연구 결과에 의하면 인진쑥과 동충하초의 투여는 사염화탄소로 유발된 간 독성을 저하시키며 혈청과 간지질 대사를 개선하는 효과가 있는 것으로 사료되며 간 기능 개선과 지질대사 개선효과를 가지고있는 인진쑥과 동충하초를 단독으로 투여하는 것보다 병행 투여하는 것이 더 효과적이며 그 혼합비율이 4% 인진쑥과 2% 동충하초에서 가장 효율적으로 나타났으나 혼합비율에 대해서는 앞으로 지속적인 연구가 필요하다고 사료된다.

• International Journal of Industrial Entomology and Biomaterials
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• 제10권1호
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• pp.35-43
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• 2005

We describe here the complete nucleotide sequence and the exon-intron structure of the Cu,Zn superoxide dismutase (SOD1) gene of Paecilomyces tenuipes and Paecilomyces sp. The SOD1 gene of P. tenuipes spans 966 bp, and consisted of three introns and four exons coding for 154 amino acid residues. Three unambiguous introns in P. tenuipes separate exons of 13, 332, 97, and 20 bp, all exhibiting exon sizes identical to Cordyceps militaris SOD1 gene. The SOD1 gene of Paecilomyces sp. contains 946 bp and consisted of four introns and five exons coding for 154 amino acid residues. Five exons of Paecilomyces sp. SOD1 are composed of 13, 180, 152, 97, and 20 bp. Interestingly, this result showed that the total length of exons 2 (180 bp) and 3 (152 bp) of Paecilomyces sp. SOD1 is same to exon 2 length (332 bp) of C. militaris SOD1 and P. tenuipes SOD1. The deduced amino acid sequence of the P. tenuipes SOD1 showed $95\%$ identity to C. militaris SOD1 and $78\%$ to Paecilomyces sp. SOD1. Phylogenetic analysis confirmed that the C. militaris SOD1, P. tenuipes SOD1 and Paecilomyces sp. SOD1 are placed together within the ascomycetes group of fungal clade.

• 한국버섯학회지
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• 제2권1호
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• pp.21-27
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• 2004

본시험은 한국에서 채집되어 강원대학교 동충하초은행(EFCC)에서 보관 중인 눈꽃동충하초의 분포와 배양적 특성을 조사하였다. 눈꽃동충하초는 한국의 산이나 계곡에서 어렵지 않게 채집할 수 있으며 골고루 분포한다. 채집시기는 7~9월로 나타났으며 9월말에 가장 많이 채집할 수 있으며 채집된 눈꽃동충하초의 종은 눈꽃동충하초(P. tenuipes)가 가장 높게 채집되었다. 눈꽃동충하초의 균사배양 최적배지는 PDA배지이며 SDAY와 HMA배지에서도 생장은 가능하며 균사생장에 가장 적합한 온도는 $25^{\circ}C$이며 pH는 6.0~7.0으로 나타났다. 적합한 영양원으로는 탄소원은 dextrose이었으며, 질소원은 tryptone가 가장 우수하였고, 무기염으로는 $KH_2PO_4$에서 가장 고른 성장을 보였다. C/N에서는 20 : 1의 비율이 가장 적합한 것으로 조사되었다.

• 동아시아식생활학회지
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• 제11권6호
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• pp.485-497
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• 2001

For a period of 5 days, the quality of breads with Paecilomyces japonica powder were investigated using chemical, sensory, and mechanical evaluations at $25^{\circ}C$. The result of analysis of chemical properties revealed that the pH value of dough was increased as the amount of Paecilomyces japonica powder increased, but volume of dough and baking loss rate were decreased. Breads with 1% and 2% Paecilomyces japonica powder showed a good overall preference in sensory evaluation. As storing time passed. moisture content, lightness, and redness were decreased in all breads, but yellowness increased. Hardness and gumminess of texture analysis were increased as storing time passed, but springiness decreased. Springiness of the mechanical properties was negatively correlated with pH value of dough and volume of bread, while was positively correlated with volume of dough and baking loss rate. In the analysis of correlation between sensory and mechanical properties showed that hardness of mechanical properties had negative correlation with softness, moistness, and springiness. Cohesiveness of mechanical properties had positive correlation with acceptability.

• 한국응용과학기술학회지
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• 제27권4호
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• pp.494-500
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• 2010

This study was carried to investigate the antidiabetic and lipid metabolism of water extract paecilomyces japonica(PJ) in Streptozotocin (STZ) induced diabetic rats. Diabetes were induced by intravenous injection of STZ at a dose of 42mg/kg dissolved in citrate buffer. The water extract of paecilomyces japonica were orally administrated once a day for 7 days at a dose of 500mg/kg or 1,000mg/kg. The contents of serum glucose, triglyceride(TG), total cholesterol were significantly decreased in PJ treated group compared to the those of STZ-control group. The content of hepatic glycogen and activities of glucose-6-phosphate dehydrogenase(G-6-PDH), glucokinase(GK) were significantly increased, but activity of glucose-6-phoshatase(G-6-Pase) was significantly decreased in PJ treated group compared to the those of STZ-control group. These results indicated that water extract of paecilomyces japonica would have antidiabetic and lipid metabolism effect in STZ-induced diabetic rats.