• Journal of Periodontal and Implant Science
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• 제32권2호
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• pp.325-338
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• 2002

Bone graft using growth factors and guided tissue regeneration have been used for the regeneration of infrabony defects which caused by periodontal disease. Calcium sulfate which is one of the resorbable barrier materials used for guided tissue regeneration. Platelet rich plasma which is a easy method to obtain the growth factors had many common points but, platelet rich plasma was still studying. This study was the comparative study between bone graft using platelet rich plasma and guided tissue regeneration using calcium sulfate barrier material in clinical view. For the study, 28 sites(2 or 3 wall infrabony defects) were treated. 14 infrabony defects were received surgical implantation of BBP-calcium sulfate composite with a calcium sulfate barrier and the others received BBP mixed with platelet rich plasma. Clinical outcome was accessed 3 and 6 months of postsurgery. 1. There was no statistical difference between CS group and PRP group in pocket depth, gingival recession, clinical attachment level, and probing bone level at baseline. 2. There was statistically significant reduction in probing depth, clinical attachment level, and probing bone level at 3 and 6 months postsurgery(p<0.05). 3. In the probing depth and clincial attachment level PPR group had less improvement than CS group, but there was no statistically difference at 3 and 6 months postsurgery. 4. In the recession PPR group had less recession than CS group, but there was no statistically difference at 3 and 6 months postsurgery. 5. In the probing bone level PPR group had less improvement than CS group, but there was no statistically difference at 6 months postsurgery. In conclusion bone graft using platelet rich plasma and guided tissue regeneration using calcium sulfate barrier showed similar clinical improvement for the treatment of 2 or 3 wall infrabony defects.

• 대한금속재료학회지
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• 제48권7호
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• pp.667-673
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• 2010

Copper filling into TSV (through-silicon-via) and reduction of the filling time for the three dimensional chip stacking were investigated in this study. A Si wafer with straight vias - $30\;{\mu}m$ in diameter and $60\;{\mu}m$ in depth with $200\;{\mu}m$ pitch - where the vias were drilled by DRIE (Deep Reactive Ion Etching) process, was prepared as a substrate. $SiO_2$, Ti and Au layers were coated as functional layers on the via wall. In order to reduce the time required complete the Cu filling into the TSV, the PPR (periodic pulse reverse) wave current was applied to the cathode of a Si chip during electroplating, and the PR (pulse-reverse) wave current was also applied for a comparison. The experimental results showed 100% filling rate into the TSV in one hour was achieved by the PPR electroplating process. At the interface between the Cu filling and Ti/ Au functional layers, no defect, such as a void, was found. Meanwhile, the electroplating by the PR current showed maximum 43% filling ratio into the TSV in an hour. The applied PPR wave form was confirmed to be effective to fill the TSV in a short time.

• 한국전산구조공학회논문집
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• 제32권5호
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• pp.297-304
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• 2019

본 논문에서는 접착영역 모델을 이용하여 2상 리튬이온 충전 시 실리콘 음극 전극의 균열진전 해석을 수행하였다. 리튬화 실리콘은 결정질 실리콘에 비해 부피가 약 3배 이상 크므로 리튬이온 충전 시 외각의 리튬화 실리콘에 매우 큰 압축력이 작용하여 압축항복이 발생한다. 리튬이온 충전 시 외각의 리튬화 실리콘은 압축항복 후에 내부의 결정질 실리콘이 리튬화 실리콘으로 상 변이하면서 발생하는 부피 팽창으로 인해 인장력이 작용한다. 이러한 인장력으로 인해 발생하는 균열진전을 접착영역 모델을 이용하여 모사하였다. 사용한 접착영역 모델은 PPR 포텐셜 기반 접착영역 모델로 하나의 포텐셜을 사용하여 복합모드에 대해서도 에너지 소산에 일관성을 지니고 있다. 유한요소 수치해석 모델로 2상 리튬이온 충전 시 모서리 균열진전을 모사한 결과가 실제 실험결과와 일치함을 확인하였고, 균열 팁에서의 최대 인장응력의 각도를 분석하여 실제 실험처럼 균열진전 방향이 회전할 것을 예측할 수 있었다.

• Journal of Veterinary Science
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• 제24권5호
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• pp.55.1-55.12
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• 2023

Background: Peste des petits ruminants (PPR), caused by the PPR virus (PPRV), is an acute and fatal contagious disease that mainly infects goats, sheep, and other artiodactyls. Peripheral blood mononuclear cells (PBMCs) are considered the primary innate immune cells. Objectives: PBMCs derived from goats were infected with PPRV and analyzed to detect the relationship between PPRV replication and apoptosis or the inflammatory response. Methods: Quantitative real-time polymerase chain reaction was used to identify PPRV replication and cytokines expression. Flow cytometry was conducted to detect apoptosis and the differentiation of CD4⁺ and CD8⁺ T cells after PPRV infection. Results: PPRV stimulated the differentiation of CD4⁺ and CD8⁺ T cells. In addition, PPRV induced apoptosis in goat PBMCs. Furthermore, apoptosis and the inflammatory response induced by PPRV could be suppressed by Z-VAD-FMK and Z-YVAD-FMK, respectively. Moreover, the virus titer of PPRV was attenuated by inhibiting caspase-1-dependent apoptosis and inflammation. Conclusions: This study showed that apoptosis and the inflammatory response play an essential role in PPR viral replication in vitro, providing a new mechanism related to the cell host response.

• Journal of Microbiology and Biotechnology
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• 제30권4호
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• pp.571-582
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• 2020

Quorum sensing (QS)-mediated infections cause severe diseases in human beings. The control of infectious diseases by inhibiting QS using antipathogenic drugs is a promising approach as antibiotics are proving inefficient in treating these diseases. Marine fungal (Pestalotiopsis sydowiana PPR) extract was found to possess effective antipathogenic characteristics. The minimum inhibitory concentration (MIC) of the fungal extract against test pathogen Pseudomonas aeruginosa PAO1 was 1,000 ㎍/ml. Sub-MIC concentrations (250 and 500 ㎍/ml) of fungal extract reduced QS-regulated virulence phenotypes such as the production of pyocyanin, chitinase, protease, elastase, and staphylolytic activity in P. aeruginosa PAO1 by 84.15%, 73.15%, 67.37%, 62.37%, and 33.65%, respectively. Moreover, it also reduced the production of exopolysaccharides (74.99%), rhamnolipids (68.01%), and alginate (54.98%), and inhibited the biofilm formation of the bacteria by 90.54%. In silico analysis revealed that the metabolite of P. sydowiana PPR binds to the bacterial QS receptor proteins (LasR and RhlR) similar to their respective natural signaling molecules. Cyclo(-Leu-Pro) (CLP) and 4-Hydroxyphenylacetamide (4-HPA) were identified as potent bioactive compounds among the metabolites of P. sydowiana PPR using in silico approaches. The MIC values of CLP and 4-HPA against P. aeruginosa PAO1 were determined as 250 and 125 ㎍/ml, respectively. All the antivirulence assays were conducted at sub-MIC concentrations of CLP (125 ㎍/ml) and 4-HPA (62.5 ㎍/ml), which resulted in marked reduction in all the investigated virulence factors. This was further supported by gene expression studies. The findings suggest that the metabolites of P. sydowiana PPR can be employed as promising QS inhibitors that target pathogenic bacteria.

• 한국지리정보학회지
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• 제17권4호
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• pp.112-128
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• 2014

본 연구에서는 구미시를 사례로 매개 변수값의 변화에 따른 2SFCA(2 Step Floating Catchment Area) 기반 접근성 변화의 지역 특성을 분석하였다. 2SFCA는 기본인구가 증가하면 접근성이 감소하고, PPR(Provider to Population Ratio)이 증가하면 접근성이 증가하는 일반적인 경향을 보였다. 구미시 내부의 지역 특성을 분석하기 위해서 민감도 분석 과정에서 군집분석을 수행하였다. 지역 특성은 다음과 같다. 첫째, 구미시 읍면 외곽지역처럼 노인복지시설과 멀리 떨어져 있는 지역은 매개 변수값의 변화에 따른 접근성 변화가 미미하였다. 둘째, 기존 노인복지시설 주변 지역은 매개 변수값의 변화에 따라 접근성이 개선되거나 약화되는 다양한 양상을 보였다. 노인인구가 많고 노인복지시설이 밀집해 있는 동 지역에서는 PPR의 변화에 따른 접근성 변화가 크고, 나머지 지역은 기본인구 변화에 따른 접근성 변화가 크게 나타났다. 셋째, 매개 변수값이 커지면 여러 곳의 노인복지시설을 이용할 수 있는 구미시 중앙구역에서 전반적으로 접근성이 양호해지는 양상을 보였다. 향후, 2SFCA 기반의 접근성 분석 과정에서 매개 변수값 선택을 위한 신중한 시나리오 적용이 필요할 것이다.

• 한국산학기술학회논문지
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• 제18권12호
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• pp.50-56
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• 2017

고층건물에서 횡력 저항의 주된 역할을 하는 외주골조는 기둥과 보를 직교시켜 구성하는 것이 일반적이었으나 최근 다양한 그리드형태의 부재배치가 이루어지고 있다. 선행연구에서는 아이소트러스 그리드를 고층건물의 외주골조로 적용한 구조적 적합성이 검토되었다. 본 연구에서는 $IsoTruss^{(R)}$ 그리드(ITG) 구조의 예비단계 설계시 외주골조 부재의 소요단면적 산정방법을 제안하였다. 모듈의 변형과 하중과의 평형조건, 허용 횡변위, 그리고 건물의 휨변형 대 전단변형의 비를 변수로 하여 소요단면적을 유도하였다. ITG 구조에서 외주골조의 부재는 횡하중에 직각인 평면(PPR), 평행한 평면(PPL), 경사진 평면(POQ)에 배치되는데 POQ에 배치된 부재는 PPR 또는 PPL에 투영시켜 부재의 강성을 반영하였다. 소요단면적은 모듈에 작용하는 전단력과 모멘트에 의해 영향을 받는데 부재사이즈 조닝을 달리하여 3가지 모델을 비교하였다. 본 연구의 효용성을 검증하기 위해 64층 건물을 설계하여 해석하였다. 최대 횡변위, 철골량, 기둥의 축력강도비를 비교분석하여 부재사이즈 조닝의 효과를 알아보았다. 제안식을 이용한 횡변위가 제한치의 약 97.3%로 나와 제안식을 설계 초기단계에서 부재사이즈의 선정에 유용하게 적용할 수 있다.

• 마이크로전자및패키징학회지
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• 제22권4호
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• pp.7-14
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• 2015

Research and application of three dimensional packaging technology in electronics have been increasing according to the trend of high density, high capacity and light weight in electronics. In this paper, TSV fabrication and research trend in three dimensional packaging are reported. Low alpha solder bumping which can solve the soft error problem in electronics is also introduced. In detail, this paper includes fabrication of TSV, functional layers deposition, Cu filling in TSV by electroplating using PPR (periodic pulse reverse) and 3 step PPR processes, and low alpha solder bumping on TSV by solder ball. TSV and low alpha solder bumping technologies need more studies and improvements, and the drawbacks of three dimensional packaging can be solved gradually through continuous attentions and researches.

• Journal of Microbiology and Biotechnology
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• 제22권9호
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• pp.1296-1300
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• 2012

The feasibility of using Deinococcus showing strong resistance to both desiccation and ionizing radiation as a dose indicator of gamma radiation exposure was evaluated. Three Deinococcus strains having different levels of radiation resistance, Deinococcus radiodurans (DRD), Deinococcus radiopugnans (DRP), and the DRD pprI mutant (DRM), were selected to develop an appropriate dose indicator for a broad range of exposures. DRD, DRP, and DRM cultures with different numbers of cells [${\sim}10^7$ to $10^3$ colony forming units (CFU)/$100{\mu}l$] were lyophilized and subjected to various doses of gamma radiation to determine a critical dose that inhibited bacterial growth completely. Finally, a combination of DRD at ${\sim}10^7$ and ${\sim}10^6$ CFU, DRP at ${\sim}10^5$ CFU, and DRM at ${\sim}10^4$ CFU successfully indicated exposure to 5, 10, 20, and 30 kGy of gamma radiation, respectively. This study shows the possibility of developing a qualitative indicator of radiation exposure using Deinococcus.

• Steel and Composite Structures
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• 제51권2호
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• pp.173-183
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• 2024

This study investigates a new seismic retrofit system that utilizes rotational friction dampers and axial springs. The retrofit system involves a steel frame with rotational friction dampers (RFD) at beam-column joints and linear springs at the corners, providing energy dissipation and self-centering capabilities to existing structures. The axial spring acts as a self-centering mechanism that eliminates residual deformations, while the friction damper mitigates seismic damage. To evaluate the seismic performance of the proposed retrofit system, a series of cyclic loading tests were carried out on a steel beam-column subassembly equipped with the proposed devices. An analytical model was then developed to validate the experimental results. A performance point ratio (PPR) was presented to optimize the design parameters of the retrofit system, and a performance-based seismic design strategy was developed based on the PPR. The retrofit system's effectiveness and the presented performance-based design approach were evaluated through case study models, and the analysis results demonstrated that the developed retrofit system and the performance-based design procedure were effective in retrofitting structures for multi-level design objectives.