• Journal of Yeungnam Medical Science
• /
• v.8 no.1
• /
• pp.154-165
• /
• 1991

To establish the hematological reference values in the healthy adults visited our hospitals, following examination were done on 2823 persons by Coulter Counter Model S-plus II ; white blood cell count: (WBC), red blood cell count(RBC), hemoglobin(Hb), hematocrit(Hct), mean corpuscular volume (MCV), mean corpuscular hemoglobin(MCH), mean corpuscular hemoglobin concentration(MCHC), red cell distribution width(RDW), platelet, plateletcrit, mean platelet volume(MPV) and platelet distribution width(PDW). The following results are obtained. 1) Male, mean value of WBC ; $6,800{\pm}2,680(2SD)/{\mu}l$ Female, mean value of WBC ; $5,950{\pm}2,380(2SD)/{\mu}l$ 2) Male, mean value of RBC ; $428{\pm}60(2SD){\times}10^4/{\mu}l$ Female, mean value of WBC ; $415{\pm}56(2SD){\times}10^4/{\mu}l$ 3) Male, mean value of Hb ; $15.4{\pm}1.8(2SD)g/dL$ Female, mean value of Hb ; $13.0{\pm}1.6(2SD)g/dL$ 4) Male, mean value of Hct ; $45.3{\pm}5.0(2SD)%$ Female, mean value of Hct ; $38.2{\pm}4.6(2SD)%$ 5) Male, mean value of MCV ; $93.8{\pm}5.8(2SD)fL$ Female, mean value of MCV ; $92.2{\pm}7.4(2SD)fL$ 6) Male, mean value of MCH ; $31.8{\pm}2.2(250)pg$ Female, mean value of MCH ; $31.4{\pm}2.8(2SD)pg$ 7) Male, mean value of MCHC ; $34.0{\pm}1.2(2SD)%$ Female, mean value of MCHC ; $33.9{\pm}1.2(2SD)%$ 8) Male, mean value of RDW ; $12.7{\pm}1.0(2SD)%$ Female, mean value of RDW ; $12.6{\pm}1.4(2SD)%$ 9) Male, mean value of Platelet ; $242.9{\pm}87.8(2SD){\times}10^3/{\mu}l$ Female, mean value of Platelet ; $242.2{\pm}89.0(2SD){\times}10^3/{\mu}l$ 10) Male, mean value of Plateletcrit ; $0.201{\pm}0.076(2SD)%$ Female, mean value of Plateletcrit ; $0.204{\pm}0.076(2SD)%$ 11) Male, mean value of MPV ; $8.20{\pm}1.70(2SD)fl$ Female, mean value of MPV ; $8.36{\pm}1.82(2SD)fl$ 12) Male, mean value of PDW ; $16.1{\pm}0.8(2SD)%$ Female, mean value of PDW ; $16.0{\pm}0.8(2SD)%$.

• Journal of Embryo Transfer
• /
• v.23 no.2
• /
• pp.87-91
• /
• 2008

This study was carried out to investigate the effects of the supplementation of glutamine, glucosamine and glutathione on the porcine oocytes on IVM rates. Cocs were incubated in NCSU-23 supplemented with at $2.0{\sim}10.0\;mM$ glucosamine, $0.5{\sim}4.0\;mM$ glutamine and $0.1{\sim}1.0\;mM$ glutathione for 48 hrs. Oocytes were transferred to 50 ul drops of maturation medium covered with mineral oil and cultured in a $CO_2$ incubator ($38^{\circ}C$, 5% $CO_2$, 95% air). The IVM rates of oocytes cultured in NCSU-23 supplemented with 0.5, 1.0, 2.0 and 4.0 mM glutamine for 48 hrs were $46.0{\pm}4.5%$, $52.0{\pm}4.8%$, $50.0{\pm}4.2%$ and $44.0{\pm}4.5%$, respectively. The IVM rates of oocytes cultured in NCSU-23 supplement with 2.0, 5.0, 7.0, 10.0 mM glucosamine for 48 hrs were $44.0{\pm}4.5%$, $42.0{\pm}4.5%$, $38.0{\pm}4.6%$ and $24.0{\pm}4.8%$, respectively. The IVM rates of oocytes cultured in NCSU-23 supplemented with glucosamine were no significantly increased compare to the control ($42.5{\pm}4.0%$). The IVM rate of oocytes cultured in NCSU-23 supplemented with 3.0, 5.0, 7.0, 10.0 mM glutathione for 48 hrs were $40.0{\pm}3.2%$, $54.0{\pm}4.2%$, $48.0{\pm}4.5%$, $44.0{\pm}4.8%$, respectively. The IVM rate of oocytes cultured in NCSU-23 supplemented with glutamine and glutathione were significantly increased co~pared to those control ($42.5{\pm}4.0%$). Glucosamine did not affect the IVM rates of oocytes. IVM rates of oocytes cultured in NCSU-23 medium for 48 hrs were significantly increased compared to the cultured for 40 hrs.

• Journal of Preventive Medicine and Public Health
• /
• v.56 no.5
• /
• pp.449-457
• /
• 2023

Objectives: This study investigated the association between baseline exposures to particulate matter with a diameter <2.5 microns (PM_2.5) and subsequent temporal changes in PM_2.5 exposure with the incidence of type 2 diabetes among Royal Thai Army personnel. Methods: A retrospective cohort study was conducted using nationwide health check-up data from 21 325 Thai Army personnel between 2018 and 2021. Multilevel mixed-effects parametric survival statistics were utilized to analyze the relationship between baseline (i.e., PM_2.5-baseline) and subsequent changes (i.e., PM_2.5-change) in PM_2.5 exposure and the occurrence of type 2 diabetes. Hazard ratios (HRs) and 95% confidence intervals (CIs) were employed to assess this association while considering covariates. Results: There was a significant association between both PM_2.5 baseline and PM_2.5-change and the incidence of type 2 diabetes in a dose-response manner. Compared to quartile 1, the HRs for quartiles 2 to 4 of PM_2.5-baseline were 1.11 (95% CI, 0.74 to 1.65), 1.51 (95% CI, 1.00 to 2.28), and 1.77 (95% CI, 1.07 to 2.93), respectively. Similarly, the HRs for quartiles 2 to 4 of PM_2.5-change were 1.41 (95% CI, 1.14 to 1.75), 1.43 (95% CI, 1.13 to 1.81) and 2.40 (95% CI, 1.84 to 3.14), respectively. Conclusions: Our findings contribute to existing evidence regarding the association between short-term and long-term exposure to PM_2.5 and the incidence of diabetes among personnel in the Royal Thai Army.

• Journal of Chest Surgery
• /
• v.33 no.9
• /
• pp.697-706
• /
• 2000

Background: Isolated lung perfusion(ILP) was developed as a new treatment approach to non-resectable primary or metastatic lung cancer, because of its ability to reduce systemic toxicity while delivering high-dose chemotherapeutic agents to the target organs. This research was planned to evaluate the direct toxic effect of high-dose cisplatin to the lung tissue during isolated lung perfusion. Material and Method: Fifteen mongrel dogs were divided in the perfusate for 40 minutes. The second group was composed of 5 mongrel dogs which underwent ILP with cisplatin 2.5 mg/Kg added to the perfusate for 30 minutes and 10 minutes with washing solution without cisplatin. The third group underwent the same procedure as the second group except cisplatin 5.0 mg/Kg in the perfusate. Activities of serum angiotensin converting enzyme(ACE), tumor necrosis factor-$\alpha$(TNF-$\alpha$), and concentration of serum lactate dehydrogenase(LDH) and blood urea nitrogen/creatinine (BUN/Cr) were analyzed in each groups at the time of pre-perfusion, 1 hour, 1 day, 1 week, and 2 weeks after ILP. Result: Serum ACE activities before and 1 hour, 1 day, 1 week, and 2 weeks after ILP in control group were 45.1$\pm$6.3, 44.6$\pm$9.3, 46.7$\pm$9.5, 50.8$\pm$9.1, 46.1$\pm$4.3 U/L. Those in cisplatin 2.5 and 5.0 mg/Kg groups were 49.4$\pm$12.6, 39.0$\pm$8.6, 42.3$\pm$15.9, 50.0$\pm$2.6, 53.8$\pm$8.3 and 55.5$\pm$12.3, 47.0$\pm$6.3, 45.1$\pm$6.9, 74.8$\pm$19.5, 60.2$\pm$12.0 U/L, respectively. Serum TNF-$\alpha$ activities in each group before and after ILP were 5.0$\pm$1.5 / 7.7$\pm$2.2 / 6.6$\pm$2.5 / 4.3$\pm$1.3 / 5.2$\pm$1.1(control), 8.7$\pm$1.6 / 9.9$\pm$2.2 / 7.9$\pm$1.5 / 6.3$\pm$2.2 / 7.4$\pm$2.4 (cisplatin 2.5 mg/Kg), and 6.9$\pm$0.7 / 8.9$\pm$3.4 / 7.9$\pm$4.0 / 3.3$\pm$0.9 / 5.8$\pm$1.3 pg/ml(cisplatin 5.0 mg/Kg). Mean LDH levels of each group were 225.7 / 271.3 / 328.9 / 350.8 / 255.7(control), 235.7 / 265.7 / 336.0 / 379.5 / 299.2 (cisplatin 2.5 mg/Kg), and 259.6 / 285.2 / 340.6 / 433.4 / 292.4 IU/L(cisplatin 5.0 mg/Kg). So there was no significant difference in serum ACE, TNF-$\alpha$, and LDH activity changes after ILP between the 3 groups. And, there was no significant changes in BUN/Cr in each groups, which was independent of ILP and perfused concentration of cisplatin. In addition, all dogs survived the ILP and there was no significant evidence of pulmonary vascular injury after 2 weeks of ILP with cisplatin. Conclusion: There was no harmful effect of cisplatin to the lund tissue of the mongrel dog up to 5.0 mg/Kg in perfusate. Therefore, it is perceived to be safe and effective to deliver high-dose cisplatin to the lung without pulmonary toxicity and renal damage with ILP.

• Asian-Australasian Journal of Animal Sciences
• /
• v.7 no.3
• /
• pp.383-387
• /
• 1994

Six heifers each of $F_1$, $F_2$, $F_3$, $F_4$ H. Friesian $\times$ Sahjwal halfbreds and pure Sahiwal with overall average initial age and live weight of $315.6{\pm}134.8 days$ and $143.8{\pm}48.5kg$, respectively were used for this study. The under trial animals were fed according to N.R.C. (1978). The overall average age at maturity, first conception and first calving was $563.8{\pm}116.8$, $675.8{\pm}135.6$ and $956.8{\pm}149.8days$ with live weights as $302.2{\pm}58.6$, $342.6{\pm}41.5$ and $433.9{\pm}38.1kg$, respectively. The overall 305 days and total milk yield for 1st lactation was $2,729.0{\pm}669.3$ and $2,992.7{\pm}377.5litre$ while the FCM (at 4%) was $2,934.2{\pm}410.8litre$. The lactation length was $336.6{\pm}69.6days$. The fat and solids not fat contents were $4.5{\pm}0.2$ and $8.2{\pm}0.3percent$, respectively. The milk production in Sahiwal cows was significantly lower than crossbred cows. The services per conception were $2.5{\pm}1.3$. The overall per head per day consumption of DM, TCP and ME was $10.9{\pm}1.2kg$, $1,399{\pm}199gm$ and $22.6{\pm}2.4 M.Cal.$, respectively. The overall milk producing efficiency for the production of one litre of FCM was $1.12{\pm}0.15kg$ of DM, $142.2{\pm}17.76gm$ of TCP and $2.31{\pm}0.27M.Cal.$ of energy while the feeding cost was Rs. $1.46{\pm}0.22$ per litre of FCM produced. The feeding cost per litre FCM was significantly higher in Sahiwal and $F_2$ than in other genetic groups.

• Journal of Embryo Transfer
• /
• v.24 no.2
• /
• pp.115-119
• /
• 2009

In the present study, effects of concentration of cryoprotectant solutions on the nuclear maturation of vitrifiedthawed porcine oocytes were examined. Oocytes were cultured in TCM-199 medium supplemented with 5% FBS at $38^{\circ}$C in 5% $C0_2$ and air. The percentage of monospermy in the toxicity group and vitrification group (22.0 ${\pm}$ 3.0% and 31.5 ${\pm}$ 3.5%) was decreased compared with that of the control group (44.0 ${\pm}$ 4.0%). The percentage of in vitro development to blastocyst in the toxicity group and vitrification group (12.0 ${\pm}$ 2.5% and 14.8 ${\pm}$ 2.8%) was decreased compared with that of the control group (28.0 ${\pm}$ 3.0%, p<0.05). The survival and in vitro developmental rate of oocytes vitrification-thawed with EDS and EDT + TCM-199 medium supplemented with 0.1% PVA were 46.3 ${\pm}$ 3.0%, 54.5 ${\pm}$ 3.8% and 14.8 ${\pm}$ 2.5%, 16.4 ${\pm}$ 2.7%, respectively. This results were lower than the control group (28.0 ${\pm}$ 3.5%). The in vitro developmental rate of embryos vitrified with EDS and EDT supplemented PVA did not have a significant difference. The survival and in vitro developmental rate of vitrified-thawed morula and blastocyst embryos were 44.2 ${\pm}$ 3.5%, 17.3 ${\pm}$ 3.0% and 48.1 ${\pm}$ 4.2%, 18.5 ${\pm}$ 3.5%, respectively. Vitrified morulae and blastcyst embryos had a lower survival and developmental rates than their control counterparts.

• Journal of Environmental Health Sciences
• /
• v.34 no.2
• /
• pp.137-147
• /
• 2008

This study evaluated the effects of the human activity and outdoor air on concentrations of size-selective particulate matters (PM) by conducting a realtime measurement in classrooms and on roofs at 4 elementary schools, 3 middle schools and 3 high schools in Incheon City. PM concentrations featured repetitive pattern of increasing during break time (including lunch hours) and cleaning time while decreasing during class hours. This trend was more prominent with inhalable PM and PM10 than fine PMs (PM2.5, PM1.0). The indoor/outdoor (I/O) ratio of inhalable PM and PM10 exceeded 1 while that of fine PMs was close to or below 1. The PM2.5 (out)/PM10 (out) ratio stood at 0.59 (${\pm}0.16$) and the PM2.5 (in)/PM10 (in) ratio was 0.29 (${\pm}0.09$), suggesting that occupant activity had a greater effect upon coarse particles (PM10-PM2.5) than upon fine particles (PM2.5, PM1.0). The correlations between the indoor and the outdoor PM concentrations showed a stronger positive correlation for fine particles than that of coarse particles. The linear regression analysis of PM concentrations indoor and outdoor indicated a higher determinant coefficient ($r^2>0.9$), and consistency for fine particles than in case of coarse particles. In conclusion, the results of this study suggest that the indoor coarse particle concentration is more attributed to occupant activity and the indoor fine particle concentration is more influenced by outdoor air pollution.

• Journal of rehabilitation welfare engineering & assistive technology
• /
• v.10 no.3
• /
• pp.207-214
• /
• 2016

The aim of this study was to evaluate Influence on intra-limb coordination in individuals wearing knee brace during walking. Seven healthy male adults ($32.3{\pm}2.7$ years old, $175.2{\pm}3.8cm$, $76.2{\pm}8.7kg$) participated. They wore knee brace or didn't wear any knee brace and were asked to walk along a 10 m long walkway. Spatiotemporal parameters, angles of the lower limbs, and intra-limb continuous relative phase (CRP) were measured and calculated. No differences of spatiotemporal parameters were shown (all p > 0.05). There were no changes in the angle and its range of motion (ROM) in the hip for the subjects as wearing knee brace, while ROM ($65.5{\pm}3.7^{\circ}$ vs. $60.5{\pm}3.5^{\circ}$, p < 0.05) of the angle and maximum flexion angles (stance: $31.9{\pm}4.6$ vs. $25.6{\pm}5.5$, swing: $76.7{\pm}3.1$ vs. $68.9{\pm}3.4$, all p < 0.05) in the knee significantly decreased. No changes in ROM of angle in the ankle were shown, whereas maximum dorsiflexion decreased ($22.4{\pm}2.6$ vs. $19.2{\pm}2.1$, p < 0.05) and maximum plantarflexion increased ($9.5{\pm}3.0$ vs. $15.7{\pm}2.2$, p<0.05). There were no changes in most of CRP between joints. CRP between the hip and knee joints decreased ($93.0{\pm}7.8$ vs, $84.7{\pm}4.9$, p < 0.05). Most of CRP standard deviation increased (between the hip and ankle joint during swing: $25.1{\pm}6.7$ vs. $32.4{\pm}1.9$, between the knee and ankle joint during stance: $46.0{\pm}12.9$ vs. $80.1{\pm}31.1$, between the knee and ankle joint during swing: $34.5{\pm}4.1$ vs. $37.6{\pm}3.1$, all p < 0.05). These results indicated that wearing knee brace affected joint angle and intra-limb coordination, but less affected gait features.

• Journal of Environmental Science International
• /
• v.23 no.2
• /
• pp.249-259
• /
• 2014

This paper investigates the characteristics of high $PM_{2.5}$ episodes occurred at Anmyeondo area in spring time, 2009. The monthly mean $PM_{2.5}$ concentration during April was the highest in the year and especially, high levels of $PM_{2.5}$ exceeding standard regulation level were sustained consecutively during 5 to 13 April. To analyze more detailed $PM_{2.5}$ characteristics, numerical simulations were carried out using CMAQ(Community Multi-scale Air Quality) with IPR(Integrated Process Rate) and DDM-3D(Decoupled Direct Method). $PM_{2.5}$ level was lower in daytime than that in nighttime due to vigorous vertical mixing during daytime. The chemical composition was showed that ratio of primary ion components such as sulfate($SO_4{^{2-}}$), nitrate($NO_3{^-}$) and ammonium($NH_4{^+}$) were nearly half of total amount of $PM_{2.5}$. Aerosol and transport process dominantly contributed to $PM_{2.5}$ concentration in Anmyeondo area and contribution rate of local emissions was nearly zero since Anmyeondo area has rare anthropogenic PM emission sources. DDM-3D analysis result showed that $PM_{2.5}$ in Anmyeondo area was influenced by emissions from Shanghai and Shandong region of China.

• Journal of Environmental Science International
• /
• v.30 no.10
• /
• pp.845-861
• /
• 2021

This study investigated the variations in monthly PM_2.5 concentrations and their characteristics at the sampling site (35.075°N, 129.080°E) around the Busan seaport area for six months (from August 2020 to January 2021). Monthly PM_2.5 concentrations in the filtered samples ranged from 8.4 to 42.3 ㎍/m³ (average=19.6±8.2 ㎍/m³, n=50) and were generally high in August, December, and January, and low in September, October, and November. The variations of monthly PM_2.5 concentrations showed similar patterns to those of the neighboring national air quality monitoring sites. The contents of Total Carbon (TC), Organic Carbon (OC), Elemental Carbon (EC), and OC/EC ratios in PM_2.5 showed large variability during the study period. The OC/EC ratios ranged from 4.2 to 34.4, suggesting that the relative contributions of OC and EC to the PM_2.5 concentrations changed temporally and might be related to their formation sources. Variations in the chemical components of and particle size distributions in PM_2.5 showed that high PM_2.5 concentrations were affected by various sources, such as sea salt and ship emission. The precursor gas concentrations were discussed in terms of monthly variations and their contributions to PM_2.5 concentrations. However, further research is needed to understand the characteristics and behaviors of PM_2.5 concentrations around the Busan seaport area.