• Title/Summary/Keyword: PLP-1

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Assessment of vitamin $B_6$ status in Korean patients with newly diagnosed type 2 diabetes

  • Ahn, Hee-Jung;Min, Kyung-Wan;Cho, Youn-Ok
    • Nutrition Research and Practice
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    • v.5 no.1
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    • pp.34-39
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    • 2011
  • The purpose of this study was to assess vitamin $B_6$ intake and status in Korean patients with newly diagnosed type 2 diabetes. Sixty-four patients with newly diagnosed type 2 diabetes and 8-11% glycated hemoglobin (A1C), along with 28 age-matched non-diabetic subjects, participated. Dietary vitamin $B_6$ intake was estimated by the 24 hour recall method and plasma pyridoxal 5'-phosphate (PLP) was measured. There was a significant difference in daily total calorie intake between the diabetic and non-diabetic groups ($1,917{\pm}376$ vs $2,093{\pm}311\;kcal$). There were no differences in intake of total vitamin $B_6$ ($2.51{\pm}0.91$ vs $2.53{\pm}0.81\;mg/d$) or vitamin $B_6$/1,000 kcal ($1.31{\pm}0.42$ vs $1.20{\pm}0.32\;mg$) between the diabetic and non-diabetic groups, and I intakes of total vitamin $B_6$ were above the Korean RDA in both groups ($180.0{\pm}57.9$ vs $179.0{\pm}65.4$). There was a higher percentage of diabetic subjects whose plasma PLP concentration was < 30 nmol/L compared to non-diabetic group. Plasma PLP levels tended to be lower in the diabetic subjects than in the non-diabetic subjects, although the difference was not statistically significant due to a large standard deviation ($80.0{\pm}61.2\;nmol/L$ vs $68.2{\pm}38.5\;nmol/L$). Nevertheless, plasma PLP levels should be monitored in pre-diabetic patients with diabetic risk factors as well as in newly diagnosed diabetic patients for long-term management of diabetes, even though this factor is not a major risk factor that contributes to the development of degenerative complications in certain patients.

Effects of protease enzyme supplementation in weanling pigs' diet with different crude protein levels on growth performance and nutrient digestibility

  • Olivier, Munezero;In Ho, Kim
    • Journal of Animal Science and Technology
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    • v.64 no.5
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    • pp.854-862
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    • 2022
  • The purpose of this study is to determine the impact of different levels of crude protein (CP) diets supplemented with dietary protease on the growth performance and nutrient digestibility of the weanling pigs. In a 5-week study, 100 crossbred ([Landrace × Yorkshire] × Duroc) of weaner pigs that have an average initial body weight (BW) of 7.17±1.06 kg were assigned to one of four dietary treatments with 5 replications and 5 pigs (3 gilts and 2 castrated male pigs) per pen in a randomized complete block design. The dietary treatments were as follows: Phase 1: CON: basal diets (20.60% CP); low protein (LP): CON - 0.30% CP; PLP1: (CON - 0.30% CP) + 0.05% protease; PLP2: (CON - 0.50% CP) + 0.05% protease. Phase 2: CON: basal diets (18.88% CP); LP: CON - 0.30% CP; PLP1: (CON - 0.30% CP) + 0.05% protease; PLP2: (CON - 0.50% CP) + 0.05% protease. The addition of protease to low CP diets significantly increased the feed conversion ratio (FCR) (p = 0.039), BW (p = 0.046), average daily gain (ADG) (p = 0.049), and average daily feed intake (ADFI) tended to increase (p = 0.053) in the young pigs during phase 1. However, FCR tended to increase throughout the experiment but did not change during phase 2, whereas BW, ADG, and ADFI stayed unchanged throughout phase 2 and overall. There was no significant difference in dry matter, nitrogen (N), and gross energy of nutrient digestibility in all phases and overall in weaned pigs with low CP when protease was fed. In contrast, adding protease to the low CP diets increased the tendency of N digestibility (p = 0.059) during phase 1. It is concluded that dietary protease supplementation tended to increase N retention during the first phase of the weaning period, hence increasing piglet performance.

Performance Analysis of BICM based DVB-T2 Receiver (BICM기반의 DVB-T2 수신기 성능분석)

  • Seo, Jeong-Wook;Kang, Min-Goo;Woo, Yong-Je
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.16 no.8
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    • pp.1575-1580
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    • 2012
  • In this paper, a DVB-T2(Digital Video Broadcasting-the 2nd Generation Terrestrial) receiver is designed under a USB-type windows environment, and the baseband frames for MPEG2-TS stream(File or Ethernet Modes) are analyzed for verifying the receiver. In addition, the performance of the BICM(Bit Interleaved Coding & Modulation) module in the receiver is analyzed in terms of PLP(Physical Layer Pipe) and L1(Layer 1) signals.

Clinical Analysis of Recurrent Intussusception and the Pathologic Lead Point in a Single Center (단일 병원에서의 재발성 장중첩증과 병적 선두에 대한 임상적 고찰)

  • Lee, Kun-Song;Park, Yun-Joon
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • v.12 no.2
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    • pp.163-170
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    • 2009
  • Purpose: Intussusception is the most common cause of intestinal obstruction in young children. Although intussusceptions are easily treated, some intussusceptions with or without a pathologic lead point (PLP) often recur. In this study, we analyzed the clinical characteristics and prognosis of recurrent intussusceptions (RI), the frequency of the PLP, and correlation between RI with PLP. Methods: The medical records of 144 patients, among 590 patients with intussusceptions who had been admitted to the Department of Pediatrics and Pediatric Surgery of Dankook University Hospital between May 1994 and June 2009 were reviewed retrospectively. Results: The overall recurrence rate of intussusceptions in this study was 9.2%. The mean interval between the initial occurrence and the first recurrent attack was 130${\pm}$175 days (range, 12 hours to 3 years). There was no statistically significant difference in the recurrence rate among patients who underwent air, barium, and manual reduction (p=0.131). Eighty-seven cases (92.6%) of RI had a successful reduction by the use of non-operative techniques. A PLP was present in 18 patients (3.0%). The most common PLP was intestinal lymphoid hyperplasia, followed by Meckel's diverticulum, duplication cyst, intestinal polyp, and adenomyoma. The mean number of intussusceptions was 4.7${\pm}$1.9 in 7 patients with PLP, which was significantly higher than (2.4${\pm}$0.9) patients without a PLP (p=0.023). The mean duration of recurrences was 17.4${\pm}$19.8 months (range, 2 days to 72 months). Conclusion: A careful search for a PLP should be performed to prevent recurrence of intussusception, especially when intussusception has recurred more than three times.

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Isolation and Characterization of Lactobacillus brevis AML15 Producing γ-Aminobutyric acid ((γ-Aminobutyric acid를 생산하는 Lactobacillus brevis AML15의 분리 및 특성)

  • Shin, Ji-Won;Kim, Dong-Geol;Lee, Yong-Woo;Lee, Hyoung-Seok;Shin, Kee-Sun;Choi, Chung-Sig;Kwon, Gi-Seok
    • Journal of Life Science
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    • v.17 no.7 s.87
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    • pp.970-975
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    • 2007
  • For the screening of ${\gamma}-aminobutyric$ acid (CABA)-producing bacteria, 86 bacterial strains which produce GABA were isolated from Kimchi and Salted fisk .Among these, three strains designated AML15, AML45-1, AML72 with relatively high GABA productivity were selecled by thin layer chromatography (TLC). To elucidate the relationship between isolated strains and the genus Lactobacillus, their 16S rDNA sequence were examined. The result of their DNA sequences showed 99% similarity with Lactobacillus brevis ATCC 367. On the basis of the these results, isolated strains were identified as Lactobacillus brevis and designated L. brevis AML15. In order to determine the optimum conditions for GABA production, the isolated strains were cultivated in pyridoxal phosphate (PLP) and monosodium glutami. acid (MSG). Results showed that L. brevis AML15 had the highest CABA productivity with 10,424 $nM/{\mu}l$ concentration in MRS broth containing 5% (w/v) MSG and 10 ${\mu}M$ PLP at pH 5.0. The results imply that L. brevis AML15 has the potential to be developed as a strain for GABA hyper-production.

Inactivation of Brain myo-Inositol Monophosphate Phosphatase by Pyridoxal-5'-Phosphate

  • Kim, Dae-Won;Hong, Joung-Woo;Eum, Won-Sik;Choi, Hee-Soon;Choi, Soo-Hyun;Kim, So-Young;Lee, Byung-Ryong;An, Jae-Jin;Lee, Sun-Hwa;Lee, Seung-Ree;Kwon, Oh-Shin;Kwon, Hyeok-Yil;Cho, Sung-Woo;Lee, Kil-Soo;Park, Jin-Seu;Choi, Soo-Young
    • BMB Reports
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    • v.38 no.1
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    • pp.58-64
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    • 2005
  • Myo-inositol monophosphate phosphatase (IMPP) is a key enzyme in the phosphoinositide cell-signaling system. This study found that incubating the IMPP from a porcine brain with pyridoxal-5'-phosphate (PLP) resulted in a time-dependent enzymatic inactivation. Spectral evidence showed that the inactivation proceeds via the formation of a Schiff's base with the amino groups of the enzyme. After the sodium borohydride reduction of the inactivated enzyme, it was observed that 1.8 mol phosphopyridoxyl residues per mole of the enzyme dimer were incorporated. The substrate, myo-inositol-1-phosphate, protected the enzyme against inactivation by PLP. After tryptic digestion of the enzyme modified with PLP, a radioactive peptide absorbing at 210 nm was isolated by reverse-phase HPLC. Amino acid sequencing of the peptide identified a portion of the PLP-binding site as being the region containing the sequence L-Q-V-S-Q-Q-E-D-I-T-X, where X indicates that phenylthiohydantoin amino acid could not be assigned. However, the result of amino acid composition of the peptide indicated that the missing residue could be designated as a phosphopyridoxyl lysine. This suggests that the catalytic function of IMPP is modulated by the binding of PLP to a specific lysyl residue at or near its substrate-binding site of the protein.

Development of Robot Simulator for Palletizing Operation Management S/W and Fast Algorithm for 'PLP' (PLP 를 위한 Fast Algorithm 과 팔레타이징 작업 제어 S/W 를 위한 로봇 시뮬레이터 개발)

  • Lim, Sung-Jin;Kang, Maing-Kyu;Han, Chang-Soo;Song, Young-Hoon;Kim, Sung-Rak;Han, Jeong-Su;Yu, Seung-Nam
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.31 no.5
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    • pp.609-616
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    • 2007
  • Palletizing is necessary to promote the efficiency of storage and shipping tasks. These are, however some of the most monotonous, heavy and laborious tasks in the factory. Therefore many types of robot palletizing systems have been developed, but many robot motion commands still depend on the teaching pendent. That is, an operator inputs the motion command lines one by one. It is very troublesome, and most of all, the user must know how to type the code. That is why we propose a new GUI (Graphic User Interface) Palletizing System. To cope with this issue, we proposed a 'PLP' (Pallet Loading Problem) algorithm, Fast Algorithm and realize 3D auto-patterning visualization interface. Finally, we propose the robot palletizing simulator. Internally, the schematic of this simulator is as follows. First, an user inputs the physical information of object. Second, simulator calculates the optimal pattern for the object and visualizes the result. Finally, the calculated position data of object is passed to the robot simulator. To develop the robot simulator, we use an articulated robot, and analyze the kinematics and dynamics. Especially, All problem including thousands of boxes were completely calculated in less than 1 second and resulted in optimal solutions by the Fast Algorithm.

Pea starch increases the dry matter flow at the distal ileum and reduces the amino acids digestibility in ileal digesta collected after 4 hours postprandial of pigs fed low-protein diets

  • Zhou, Junyan;Wang, Lu;Yang, Guangxin;Yang, Lijie;Zeng, Xiangfang;Qiao, Shiyan
    • Animal Bioscience
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    • v.35 no.7
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    • pp.1021-1029
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    • 2022
  • Objective: The study was aimed to investigate the rules of postprandial changes in intestine digesta dry matter (DM) flow and amino acid digestibility of growing pigs fed low-protein (LP) diets made of different starch. Methods: Eight barrows (28.8±2.1 kg) with a T-cannula at the distal ileum were randomly allotted to an 8×3 Youden square design. Treatments included: waxy corn starch LP (WLP); corn starch LP (CLP) and pea starch LP (PLP). Diets were given at 08:00 and 20:00. Digesta samples were collected in six 2-h stages from 08:00 to 20:00. Results: The Cr concentrations of ileal digesta increased and then decreased in WLP and CLP, while increased continuously in PLP as time passed after postprandial (p<0.05). Higher average Cr concentrations (0.78% and 0.84% vs 0.70%; p<0.05) and lower average DM flow (181.1 g/kg and 166.3 g/kg vs 240.3 g/kg; p<0.001) were observed in WLP and CLP, compared with PLP. The apparent ileal digestibility coefficient of most amino acids in WLP and CLP increased compared with that in PLP. No difference in lysine or methionine digestibility was observed. When digesta were collected in 2-h periods, the apparent ileal digestibility coefficient of amino acids did not change over time. When digesta was collected in 4-h periods from 16:00 to 20:00 and 6-h periods from 14:00 to 20:00 (p<0.05), WLP and CLP showed markedly higher amino acid digestibility than PLP Conclusion: High-amylose slowly digested starch can increase the DM flow at the distal ileum and reduce the apparent ileal digestibility coefficient of amino acids of pigs fed LP diets. Compared with waxy corn starch and corn starch, pea starch reduced the digestibility of amino acids in digesta collected after 4 h postprandial.