• The Journal of the Korean Society for Microbiology
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• v.21 no.1
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• pp.17-31
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• 1986

Forty clinical isolates of Serratia marcescens were tested for their susceptibility to 19 antimicrobial drugs and studied on the molecular characteristics of R plasmids. Cefotaxime (Ct) was the most effective drug and only 2 (5%) strains were resistant to this drug. Thirteen to 18% of strains were resistant to cefoperazone (Cz), amikacin (Ak). and trimethoprim (Tp), and 28 to 40% were resistant to piperacillin (Pi), nalidixic acid (Na), gentamicin (Gm), and cefoxitin (Cx). A majority of strains were resistant to carbenicillin (Cb), tobramycin (Tp), kanamycin (Km), and cefamandole (Cd), and all to cephalothin. One half of the isolates were resistant to 10 or more drugs. $MIC_{90}$ of Pi to Gm-resistant strains (Gm') were 8 times higher than that to Gm-susceptible strains (Gm'), but $MIC_{90}$ of Ak, Cx, Ct, and Cz were almost the same between both Gm' and Gm' strains. Nine (23.7%) strains among 38 of multiply drug-resistant S. marcescens transferred conjugally their partial patterns of resistance to E. coli or Klebsiella strains, and two S. marcescens strains producing bacteriocin transferred their resistance to Klebsiella only, but not to E. coli. The plasmid profiles of S. marcescens were studied by the methods of SDS lysis and agarose gel electrophoresis. Twenty-four (60%) strains carried one to four plasmids of 1.4. to 144 Mdal, and conjugative R plasmids of 49 to 127 Mdal were noted in transconjugants. MIC levels of drugs in transconjugants were variable by the R plasmids and recipient strains.

• Journal of Radiation Industry
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• v.4 no.3
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• pp.209-213
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• 2010

Bacterial blight is one of the most serious diseases of rice (Oryza sativa L.), and it has been known that Xanthomonas oryzae pv. oryzae (Xoo) causes this disease symptom. To develop resistance rice cultivars against Xoo, 3,000 lines of $M_3$ mutants, which were irradiated with gamma ray, were tested by 'scissor-dip method' primarily, and 191 putative resistant lines were selected. In $M_4$ generation, these lines were screened again with various ways such as measuring of symptom of bacterial blight in leaf, number of tiller, fresh weight, and phenotypic segregation ratio in next generation. Finally, six resistance lines were selected. RT-PCR analysis revealed that these lines displayed high level of R-genes such as Xa21, Pi36, and Pi-ta. These results indicate that mutations by gamma ray cause disruptions of regulatory signal transduction systems of these R-genes. Furthermore, these selected mutants could be useful for the development of rice cultivar resistant to Xoo.

• The Transactions of the Korean Institute of Electrical Engineers B
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• v.53 no.9
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• pp.552-561
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• 2004

This paper is concerned with a new speed sensorless induction motor scheme which can be successfully applied to at any speed including even zero speed. The proposed method is robust against rotor resistance variations. In addition, simultaneous on-line estimations of speed and rotor resistance are realized based on a feedforward type torque control approach. The rotor flux with a low frequency sinusoidal waveform has been utilized to help the simultaneous estimation for both speed and rotor resistance. The control scheme has no current minor loop to determine voltage references. Since the proposed estimation does not depend on any derivative terms of currents and stator voltages, it offers a good performance at extremely low speed region for sensorless induction motor. Furthermore, the proposed control is simply using motor parameters and stator currents without determining any PI gains for current feedback and any signal injection for the rotor resistance estimation. Finally, both simulation and experimental results are given to show the effectiveness of this method.

• Biomolecules & Therapeutics
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• v.26 no.3
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• pp.313-321
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• 2018

Anti-cancer drug resistance is a major problem in colorectal cancer (CRC) research. Although several studies have revealed the mechanism of cancer drug resistance, molecular targets for chemotherapeutic combinations remain elusive. To address this issue, we focused on the expression of cellular prion protein ($PrP^C$) in 5-FU-resistant CRC cells. In 5-FU-resistant CRC cells, $PrP^C$ expression is significantly increased, compared with that in normal CRC cells. In the presence of 5-FU, $PrP^C$ increased CRC cell survival and proliferation by maintaining the activation of the PI3K-Akt signaling pathway and the expression of cell cycle-associated proteins, including cyclin E, CDK2, cyclin D1, and CDK4. In addition, $PrP^C$ inhibited the activation of the stress-associated proteins p38, JNK, and p53. Moreover, after treatment of 5-FU-resistant CRC cells with 5-FU, silencing of $PrP^C$ triggered apoptosis via the activation of caspase-3. These results indicate that $PrP^C$ plays a key role in CRC drug resistance. The novel strategy of combining chemotherapy with $PrP^C$ targeting may yield efficacious treatments of colorectal cancer.

• Journal of Yeungnam Medical Science
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• v.30 no.1
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• pp.4-9
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• 2013

Leptin, a 16-kDa cytokine, is secreted by adipose tissue in response to the surplus of fat store. Thereby, the brain is informed about the body's energy status. In the hypothalamus, leptin triggers specific neuronal subpopulations (e.g., POMC and NPY neurons) and activates several intracellular signaling events, including the JAK/STAT, MAPK, PI3K, and mTOR pathway, which eventually translates into decreased food intake and increased energy expenditure. Leptin signal is inhibited by a feedback inhibitory pathway mediated by SOCS3. PTP1B involves another inhibitory pathway of leptin. Leptin potently promotes fat mass loss and body weight reduction in lean subjects. However, it is not widely used in the clinical field because of leptin resistance, which is a common feature of obesity characterized by hyperleptinemia and the failure of exogenous leptin administration to provide therapeutic benefit in rodents and humans. The potential mechanisms of leptin resistance include the following: 1) increases in circulating leptin-binding proteins, 2) reduced transport of leptin across the blood-brain barrier, 3) decreased leptin receptor-B (LRB), and/or 4) the provocation of processes that diminish cellular leptin signaling (inflammation, endoplasmic reticulum stress, feedback inhibition, etc.). Thus, interference of the cellular mechanisms that attenuate leptin signaling improves leptin action in cells and animal models, suggesting the potential utility of these processes as points of therapeutic intervention. Various experimental trials and compounds that improve leptin resistance are introduced in this paper.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.64 no.8
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• pp.1217-1223
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• 2015

In this paper, we study the problem of torque ripple minimization in Brushless DC Motors (BLDC) and proposes a disturbance observer (DOB) based controller in order to efficiently reduce the torque ripple. In the DOB based control system, an equivalent disturbance (plant disturbance and effect of modelling error) is cancelled by its estimate. When the DOB controller is applied to BLDC motors, the effect of inverter switching is considered as an equivalent disturbance and to be cancelled by the DOB controller. Through computer simulations, it is shown that the performance of the proposed DOB controller is superior to that of the conventional PI controller. In the case where the numerical values of resistance and inductance are not known exactly, it is shown that the proposed DOB controller achieves better performance than the PI controller.

• Journal of Electrochemical Science and Technology
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• v.10 no.2
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• pp.177-184
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• 2019

An electrochemical detection method for capsaicin has been developed using ionic liquid (IL) doped graphene-titania-Nafion composite-modified electrode. The combination of IL (1-hexyl-3-methylimidazolium with hexafluorophosphate counter ion) in the composite-modified electrode resulted in a significantly increased electrochemical response for capsaicin compared to that obtained at the corresponding electrode without IL. The increased electrochemical signal could be ascribed to the decreased electron transfer resistance through the composite film and also to the effective accumulation of capsaicin on the electrode surface due to ${\pi}-{\pi}$ interaction of the imidazole groups of IL with the aromatic rings of capsaicin. The present IL composite-modified electrode can detect capsaicin with a concentration range from $3.0{\times}10^{-8}M$ to $1.0{\times}10^{-5}M$ with a detection limit of $3.17{\times}10^{-9}M$ (S/N = 3). The present sensor showed good reproducibility (RSD = 3.2%).

• Journal of Electrical Engineering and Technology
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• v.8 no.3
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• pp.530-543
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• 2013

This paper describes a control scheme of speed sensorless fuzzy direct torque control (FDTC) of permanent magnet synchronous motor for electric vehicle (EV). Electric vehicle requires fast torque response and high efficiency of the drive. Speed sensorless FDTC In-wheel PMSM drives without mechanical speed sensors at the motor shaft have the attractions of low cost, quick response and high reliability in electric vehicle application. This paper presents a new approach to estimate the speed of in-wheel electrical vehicles based on Model Reference Adaptive System (MRAS). The direct torque control suffers in low speeds due to the effect of changes in stator resistance on the flux measurements. To improve the system performance at low speeds, a PI-fuzzy resistance estimator is proposed to eliminate the error due to changes in stator resistance. High performance sensorless drive of the in-wheel motor based on MRAS with on line stator resistance tuning is established for four motorized wheels electric vehicle and the whole system is simulated by matalb/simulink. The simulation results show the effectiveness of the new control strategy. This proposed control strategy is extensively used in electric vehicle application.

• Korean Journal of Breeding Science
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• v.50 no.4
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• pp.424-433
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• 2018

Watermelon (Citrullus lanatus) is an economically important vegetable crop all over the world, which has functional compounds such as lycopene and citrulline. Gummy stem blight caused by Didymella bryoniae is one of the most devastative diseases in watermelon. Single nucleotide polymorphisms (SNPs), which are genetic variations occurring between individuals with respect to a single base, were often used to construct genetic linkage maps and develop molecular markers linked to a variety of horticultural traits and resistance to several diseases. In this study, we developed high-resolution melting (HRM) markers based on SNPs generated from NGS resequencing of two parents in watermelon. Plant materials were C. lanatus '920533' (female and susceptible parent), C. amarus 'PI 189225' (male and resistant parent), and their $F_1$ and $F_2$ progenies. A total of 13.6 Gbp ('920533') and 13.1 Gbp ('PI 189225') of genomic sequences were obtained using NGS analysis. A total of 6.09 million SNPs between '920533' and 'PI 189225' were detected, and 354,860 SNPs were identified as potential HRM primer sets. From these, a total of 330 primer sets for HRM analysis were designed. As a result, a total of 61 HRM markers that have polymorphic melting curves were developed. These HRM markers can be used for the construction of SNP-based linkage maps and for the analysis of quantitative trait loci (QTLs) related to gummy stem blight resistance.

• Journal of Korean Society of Forest Science
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• v.50 no.1
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• pp.25-28
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• 1980

The purpose of the this study was to compare the torlent tree to drought among the Pinus koraiensis, Abies holophylla, and Ginkgo biloba. The water relations parameters of leafy shoots were measured by the Pressure chamber technique from August 10 to September 4 in 1980. On the water relations parameters such as original osmotic pressure(${\pi}_0$), osmotic pressure(${\pi}_p$) and relative water content (RWC) at incipient plasmolysis, and Vp/Vo ratio of the volume of osmotic water (Vp) at incipient plasmolysis to total symplasmic water (Vo) basis, the Abies holophylla shoots showed ontogenetically a superior osmoregulation which are closely associated with drought resistance compared with Pinus koraiensis, and the Ginkgo biloba shoots showed the heighest among these three species.