Journal of Radiation Industry (방사선산업학회지)

Korean Society of Radiation Industry (한국방사선산업학회)
권호 표지

Screening of Gamma Radiation-Induced Pathogen Resistance Rice Lines against Xanthomonas oryzae pv. oryzae

방사선을 이용한 벼 흰잎마름병 저항성 돌연변이 벼 계통의 선발

  • Lim, Chan Ju (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Lee, Ha Yeon (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kim, Woong Bom (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Ahmad, Raza (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Moon, Jae Sun (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kim, Dong Sub (Department of Food and Plant Breeding Research, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kwon, Suk-Yoon (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
  • 임찬주 (한국생명공학연구원 식물시스템공학연구센터) ;
  • 이하연 (한국생명공학연구원 식물시스템공학연구센터) ;
  • 김웅범 (한국생명공학연구원 식물시스템공학연구센터) ;
  • 아마드 라자 (한국생명공학연구원 식물시스템공학연구센터) ;
  • 문제선 (한국생명공학연구원 식물시스템공학연구센터) ;
  • 김동섭 (한국원자력연구원 정읍방사선과학연구소 방사선식품육종연구부) ;
  • 권석윤 (한국생명공학연구원 식물시스템공학연구센터)
  • Received : 2010.07.30
  • Accepted : 2010.08.17
  • Published : 2010.09.30

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Abstract

Bacterial blight is one of the most serious diseases of rice (Oryza sativa L.), and it has been known that Xanthomonas oryzae pv. oryzae (Xoo) causes this disease symptom. To develop resistance rice cultivars against Xoo, 3,000 lines of $M_3$ mutants, which were irradiated with gamma ray, were tested by 'scissor-dip method' primarily, and 191 putative resistant lines were selected. In $M_4$ generation, these lines were screened again with various ways such as measuring of symptom of bacterial blight in leaf, number of tiller, fresh weight, and phenotypic segregation ratio in next generation. Finally, six resistance lines were selected. RT-PCR analysis revealed that these lines displayed high level of R-genes such as Xa21, Pi36, and Pi-ta. These results indicate that mutations by gamma ray cause disruptions of regulatory signal transduction systems of these R-genes. Furthermore, these selected mutants could be useful for the development of rice cultivar resistant to Xoo.

Keywords

Acknowledgement

Supported by : 농림수산식품부