• Journal of the Korean Society of Environmental Restoration Technology
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• v.12 no.6
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• pp.112-122
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• 2009

Payment of Forest Landscape Service (PFLS) is based on the value of landscape conservation and is a positive forest policy inducing the owners of mountains to improve environmental service quality with economic incentives. The purpose of this study is to test the feasibility of PFLS and find out the elements related to PFLS such as associated statutes, target applications, eligible owner's requirements, and applicable environmental services. Research sites were selected in designated reserved forests by law and surveys were carried out with 28 professional forestry engineers and 10 owners of reserved forests located in Chungnam Province in November, 2008. As a result, the owners are willing to participate pilot-project of PFLS if they could have tax incentives. Preferred activities in their forestry are eco-tourism and carbon emission trading as PFLS business model. Although they expect low economic benefit from the PFLS, respondents answered introducing PFLS will give good opportunities for owners of a reserved forest to enhance willingness to manage their forestry properly for the landscape conservation. In this study, PFLS evaluation indicators and policy directions are established and recommends the strategies to cope with changing needs of forestry conservation by inducing the owners' active participation in the sustainable forest landscape management.

• Korean Journal of Medicinal Crop Science
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• v.17 no.1
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• pp.33-38
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• 2009

The full-length cDNA encoding Perilla frutescens limonene synthase (PFLS) (603 amino acids, GenBank accession no. D49368) was cloned. To elucidate the role of PFLS in gene regulation, we transiently transformed full-length PFLS into tobacco plants. PFLS mRNA was first detected in the intact leaves of the plants at 6 h, and the LS transcript level increased after 12 h in leaves treated with oxidative stress-related chemicals. The transient overexpression of PFLS resulted in increased transcription of NbPR1 and NbSIP in Nicotiana benthamiana leaves. Thus, our result confirmed that the infiltration of PFLS gene act as a transcriptional regulator of NbPR1 or NbSIP genes in the tobacco.

• Proceedings of the KIPE Conference
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• 2001.10a
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• pp.132-135
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• 2001

The design of square pulse generators using pulse forming lines (PFLs) made up of identical L and C, was reviewed in this study. 14 different types of PFLs were analyzed utilizing PSpice simulation results. These PFLs were characterized with respect to their distinct features: the number of forming lines (single or double), the circuit relationship between PFL and load (parallel or series), the types of energy storage (voltage source, current source or a combination of both). The characteristic impedances, output parameters such as pulse width, voltage and current magnitudes, and powers were derived for each scheme. The merits and demerits of the output parameters were also included.

• Journal of Applied Biological Chemistry
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• v.62 no.1
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• pp.1-6
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• 2019

The flavonoid, isokaempferide, has various biological activities such as hepatoprotective, antimicrobial and antiproliferative effect and is extracted from Amburana cearensis and Cirsium rivulare (Jacq.). Biotransformation is an alternative tool for the synthesis of value-added flavonoids with inexpensive substrates. Here, to synthesize isokaempferide from naringenin, two genes, PFLS and Rice O-mthyltransferae-9 were introduced in Escherichia coli. Although isokaempferide was successfully synthesized, the amount of biosynthesis was no high. In order to increase the yields of isokaempferide, S-adenosylmethionine (SAM) used as a methyl donor was increased by deleting MetJ, which is a transcriptional regulator related to SAM biosynthetic pathway. Next we optimized the cell concentration and substrate feed concentration with the engineered E. coli strain. Through these strategies, the biosynthesis of isokaempferide was increased up to 87 mg/L.

• Korean Journal of Clinical Laboratory Science
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• v.55 no.3
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• pp.143-150
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• 2023

The global outbreak of COVID-19 has underscored the pressing need for robust infection control practices in pulmonary function laboratories (PFL). However, the existing guidelines and regulatory frameworks provided by relevant authorities in the country have revealed certain deficiencies in effectively addressing this significant public health crisis. This study surveyed the infection control regulations, disposable item usage, ventilation facilities, spatial separation, and the configuration of entrance doors in 51 domestic hospital facilities from Oct 1, 2021, to Nov 2, 2021. The survey findings revealed that while there was a relatively satisfactory adherence to airborne, droplet, and contact precautions with adequate awareness and utilization of personal protective equipment, the environmental disinfection practices exhibited a suboptimal performance rate of 39.22% per patient. Depending on the specific survey domains, substantial variations were observed in the utilization of disposable items (81.05%), ventilation systems (45.75%), dedicated testing spaces (80.39%), separation of administrative areas (15.69%), and the installation of automated doors (19.61%). This study not only highlights the paramount importance of infection control in PFLs within domestic medical institutions but also provides foundational data for developing and enhancing standardized guidelines that align with international benchmarks for infection control in these settings.