• 제목/요약/키워드: PC 법

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Phylogenetic Characteristics of viable but Nonculturable Bacterial Populations in a Pine Mushroom (Tricholoma matsutake) Forest Soil (송이 자생군락 토양 내 난배양성 세균군집의 계통학적 특성)

  • Kim, Yun-Ji;Whang, Kyung-Sook
    • Korean Journal of Microbiology
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    • 제43권3호
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    • pp.201-209
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    • 2007
  • The CFDA (6-carboxyfluorescein diacetate) direct viable count method and plate count (PC) method using conventional nutrient broth (NB) medium and $10^{-2}$ diluted NB (DNB) medium were applied to samples collected from Mt. Yongdoo In Andong, in an effect to determine the number of living bacteria pine mushroom forest soil. The number of living bacteria determined via plate count in NB medium comprised $5{\sim}8%$ of the CFDA direct viable count, and the bacteria in the DNB medium comprised $40{\sim}47%$. This result indicated that viable but nonculturable (VBNC) bacteria existed in the pine mushroom forest soil at a high percentage. The phylogenetic characteristics of the VBNC bacterial populations in the samples of pine mushroom (Tricholoma matsutake) forest soil were analyzed via the direct extraction of DNA and 16S rDNA-ARDRA. The 115 clones from pine mushroom forest soil were clustered into 31 different RFLP phylotypes by ARDRA. Based on the 16S rDNA sequences, the 31 ARDRA clusters were classified into 6 phylogenetic groups: ${\alpha}-,\;{\beta}-,\;{\gamma}-Proteobacteria$, Acidobacteria, Actinobacteria and Firmicutes. Among these bacterial populations, approximately 85% were classified as members of phylum Acidobacteria. The Acidobacteria phylum was shown to exist abundantly in the pine mushroom forest soil.

A Study on the Molecular Weight Control and Rheological Properties of Branched Polycarbonate (분지형 폴리카보네이트의 분자량 조절 및 유변학적 특성 연구)

  • Lee, Bom Yi;Dahal, Prashanta;Kim, Hee Seung;Yoo, Seung Yoon;Kim, Youn Cheol
    • Applied Chemistry for Engineering
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    • 제23권4호
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    • pp.388-393
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    • 2012
  • Branched polycarbonates (B-PCs) were synthesized using melt polymerization method with four different end capping agents and vaying concentrations. The chemical structure of the synthesized PC was determined by FT-IR and $^{1}H-NMR$ spectroscopy, and the reaction of the end capping agent was confirmed by the existence of hydroxy group in FT-IR spectrum. The average molecular weight and distribution, glass transition and degradation temperatures were determined by GPC, DSC and TGA. The average molecular weight changed with the chemical structure of end capping agent, and 4-tert-butylphenol was estimated as the optimum end capping agent. The average molecular weights of B-PCs decreased with the increase of the concentration of the agent, the number average molecular weight represented 20000 when 0.05 mol% of 4-tert-butylphenol was added to B-PCs. The melt viscosities of the B-PCs decreased with the decrease of the molecular weight of B-PCs, and adding of the agent was not effected to shear thinning tendency.

Teachers' Perceptions about Priority and Hinderance of Science Objectives (교사들이 인식하는 과학과 목표의 영역별 중요도와 장애요인)

  • Bae, Sung-Yuhl;Park, Yune-Bae
    • Journal of The Korean Association For Science Education
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    • 제20권4호
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    • pp.572-581
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    • 2000
  • Teachers' perceptions about curricular objectives are very important to achieve given educational objectives. This study examined teachers' perceptions about priority and hinderance of science objectives. The national science curriculum stated four areas of objectives: inquiry, knowledge, attitude, and STS. A total of 76 elementary and 217 secondary teachers were responded to questionnaire developed for this study. Non-parametric statistics, such as Friedman test, Kruskal-Wallis test, and chi-square test were used by using SPSS/PC program. Teachers in general ranked the inquiry first among the four areas at their perception on the national curriculum, on themselves, and on their instructions. Elementary teachers ranked inquiry higher than secondary science teachers, while secondary science teachers ranked knowledge higher than elementary teachers. As factors hindering teachers from achiving the objectives, elementary teachers pointed out overcrowded classroom and secondary science teachers pointed out college and university entrance examination respectively.

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Comparison of Biological Activities of Opuntia humifusa and Opuntia ficus-indica (손바닥선인장의 생리활성 비교 연구)

  • Park, Chul Min;Kwak, Byoung Hee;Park, Si Hyung;Kim, Hui;Rhyu, Dong Young
    • Korean Journal of Plant Resources
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    • 제26권5호
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    • pp.519-525
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    • 2013
  • The Opuntia extract has been traditionally used to treat diabetes, inflammation, and rheumatoid arthritis in oriental medicine. The purpose of this study is to investigate characteristics of biological activity between Opuntia humifusa and Opuntia ficus-indica which is cultivating in korea using cell-free system or cell-based assay. O. humifusa extract effectively inhibited ${\alpha}$-glucosidase activity or improved the immune function, and its biological activity was more effective than O. ficus-indica extract. The scavenging activity of DPPH radical and the inhibitory effect of tyrosinase similarly showed by O. humifusa and O. ficus-indica extract, however neuroprotective effect only showed a tendency to increase compared with control in PC12 cells. Therefore the results suggest that O. humifusa can be a useful agent for treatment of diabetes and immunodeficiency.

The Effect of Daucus carota L. Extracts on the Fluidity of Phospholid Liposomes (당근추출물이 인지질막 Liposome의 유동성에 미치는 영향)

  • 신미옥;배송자
    • Journal of the Korean Society of Food Science and Nutrition
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    • 제30권4호
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    • pp.646-650
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    • 2001
  • In this study, we investigated the thermotropic behavior of Daucus carota L. (DCS) extracts in phosphatidylcholine(PC) liposomes using high-sensitivity differential scanning calorimetry (nano-DSC). We used dipalmitoylphosphatidylcholine (DPPC) bilayers which made most stable liposomes among the other phosphatidylcholine. The sample DCS was extracted and fractionated to four different types, hexane(DCSMH), ethylacetate (DCSMEA), butanol (DCSMB) and aqueous(DCSMA) fractions. Compared to the other fractions of Daucus carota L., the DCSMH and DCSMEA fractions markedly affected the thermotropic properties of DPPC liposomes, broadened and shifted the thermograms of transition to lower temperatures. The incorporation of DCSMH and DCSMEA in DPPC liposomes were preferentially located in the hydrophobic core of DPPC bilayers, where it reduced the lipid packing orderness (cooperative unit) in the gel state compared to it in the liquid-crystalline state. These results suggest that the activities of the Daucus carota L. extracts to enhance the fluidity of the liposomal membrane have implication in their biological activities.

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Comparative Study of Postoperative Analgesic Effect of IV-PCA According to Timing of Infusion in Patients with Total Abdominal Hysterectomy (자궁적출술 환자의 진통제 투여시작 시기에 따른 수술 후 통증호소 비교(IV-Patient Controlled Analgesia를 이용하여))

  • Park Jeong-Ok;Lee Pyung-Ae;Cho You-Sook;Park Mi-Mi;Kim Hye-Sook;Park Jee-Won;Min Sang-Kee
    • Journal of Korean Academy of Fundamentals of Nursing
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    • 제9권2호
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    • pp.323-334
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    • 2002
  • Purpose: This study was designed to verify preemptive effects of intravenous patient-controlled analgesia (IV-PCA) infusion on postoperative pain in women having a total abdominal hysterectomy. Method: The research design was a nonequivalent control group post test only design. The participants in this study were 50 women who were scheduled for a total abdominal hysterectomy at a University affiliated Hospital in Suwon, Korea. The subjects were divided into two groups. For the experimental group, IV-PCA infusion was started before the skin incision and for the control group. IV-PCA infusion was started after the skin was closed. Each group was evaluated in terms of pain score by the visual analogue scale (VAS) and the number of times they pushed the button for IV-PCA at postoperative hours 1, 2, 3, 6, 12, and 24. The data were collected from July 1 to December 10, 2001. Collected data was analyzed by SPSS/PC + program. Result: 1. There was no difference between the two groups, over six points for the number of times the control button for IV-PCA was used. Group differences and interaction effect were not significant. 2. There was no significant difference in pain scores between the two groups, over seven time points. A significant interaction effect was observed between groups and measurement Points in time. 3. There was a significant difference in the requirements for additional analgesia between the two groups, 32% of the control group received additional analgesia. Conclusion: Preemptive analgesics administration may have a better effect in relieving postoperative pain than the usual analgesic treatment which is started after surgery.

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Methods for Stabilizing QoS in Mobile Cloud Computing (모바일 클라우드 컴퓨팅을 위한 QoS 안정화 기법)

  • La, Hyun Jung;Kim, Soo Dong
    • KIPS Transactions on Software and Data Engineering
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    • 제2권8호
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    • pp.507-516
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    • 2013
  • Mobile devices have limited computing power and resources. Since mobile devices are equipped with rich network connectivity, an approach to subscribe cloud services can effectively remedy the problem, which is called Mobile Cloud Computing (MCC). Most works on MCC depend on a method to offload functional components at runtime. However, these works only consider the limited verion of offloading to a pre-defined, designated node. Moveover, there is the limitation of managing services subscribed by applications. To provide a comprehensive and practical solution for MCC, in this paper, we propose a self-stabilizing process and its management-related methods. The proposed process is based on an autonomic computing paradigm and works with diverse quality remedy actions such as migration or replicating services. And, we devise a pratical offloading mechanism which is still in an initial stage of the study. The proposed offloading mechanism is based on our proposed MCC meta-model. By adopting the self-stabilization process for MCC, many of the technical issues are effectively resolved, and mobile cloud environments can maintain consistent levels of quality in autonomous manner.

Fast Planar Shape Deformation using a Layered Mesh (계층 메쉬를 이용한 빠른 평면 형상 변형)

  • Yoo, Kwang-Seok;Choi, Jung-Ju
    • Journal of the Korea Computer Graphics Society
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    • 제17권3호
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    • pp.43-50
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    • 2011
  • We present a trade-off technique for fast but qualitative planar shape deformation using a layered mesh. We construct a layered mesh that is embedding a planar input shape; the upper-layer is denoted as a control mesh and the other lower-layer as a shape mesh that is defined by mean value coordinates relative to the control mesh. First, we try to preserve some shape properties including user constraints for the control mesh by means of a known existing nonlinear least square optimization technique, which produces deformed positions of the control mesh vertices. Then, we compute the deformed positions of the shape mesh vertices indirectly from the deformed control mesh by means of simple coordinates computation. The control mesh consists of a small number of vertices while the shape layer contains relatively a large number of vertices in order to embed the input shape as tightly as possible. Since the time-consuming optimization technique is applied only to the control mesh, the overall execution is extremely fast; however, the quality of deformation is sacrificed due to the sacrificed quality of the control mesh and its relativity to the shape mesh. In order to change the deformation behavior and consequently to compensate the quality sacrifice, we present a method to control the deformation stiffness by incorporating the orientation into the user constraints. According to our experiments, the proposed technique produces a planar shape deformation fast enough for real-time applications on limited embedded systems such as cell phones and tablet PCs.

Impact of Participation in Sport for All on Leisure Satisfaction, Psychological Wellbeing and Subjective Wellbeing (생활체육 참여활동이 여가만족과 심리적, 주관적 웰빙에 미치는 영향)

  • Moon, Tae-Young;Heo, Chung-Uk
    • Journal of the Korea Academia-Industrial cooperation Society
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    • 제12권9호
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    • pp.3984-3993
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    • 2011
  • The purpose of this study was to examine the impact of participation in sport for all on leisure satisfaction, psychological wellbeing and subjective wellbeing in an attempt to make a contribution to the leisure satisfaction, psychological wellbeing and subjective wellbeing of people enjoying sport for all. The subjects in this study were 200 people who were selected by convenience sampling from a population of male and female adults who participated in sport for all in Gangwon Province. The statistical package SPSS 11.0 was employed to make a frequency analysis, reliability analysis, factor analysis and multiple regression analysis. The findings of the study were as follows: First, participation in sport for all had a statistically significant impact on leisure satisfaction. Those who more participated in sport for all expressed more satisfaction with their leisure life. Second, leisure satisfactionexerted a statistically significant influence on psychological wellbeing. Third, leisure satisfaction affected subjective wellbeing in a statistically significant way. The findings of the study indicated that the leisure satisfaction of those who participated in sport for all exercised a great physical and mental influence on themselves. Those who don't participate in sport for all should steadily be advised to do that, and the national and local governments should develop a wide variety of sport-for-all programs to let everybody lead a better life by enjoying sport for all.

Analysis on the Rigid Connections of the Drilled Shaft with the Cap for Multiple Pile Foundations (현장타설말뚝을 적용한 다주식 기초에서 말뚝과 캡의 강결합에 대한 분석)

  • Cho, Sung-Min
    • Journal of the Korean Geotechnical Society
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    • 제24권7호
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    • pp.61-73
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    • 2008
  • Piles of a bridge pier are connected with the column through the pile cap (footing). Behavior of the pile foundation can be different according to the connection method between piles and the pile cap. Connection methods between pile heads and the pile cap are divided into two groups : rigid connections and hinge connections. Domestic design code has been specified to use rigid connection method for the highway bridge. In the rigid connection method, maximum bending moment of a pile occurs at the pile head and this helps the pile to prevent the excessive displacement. Rigid methods are also good to improve the seismic performance. However, some specifications prescribe that conservative results through investigations of both the fixed-head condition and the free-head condition should be reflected in the design. This statement may induce an over-estimated design for the bridge which has high-quality structures with casing covered drilled shafts and the PC-house contained pile cap. Because the assumption of free-head conditions (hinge connections) is unreal for the elevated pile cap system with multiple piles of the long span sea-crossing bridges. On the other hand, elastic displacement method to evaluate the pile reactions under the pile cap is not suitable for this type of bridges due to impractical assumptions. So, full modeling techniques which analyze the superstructure and the substructure simultaneously should be performed. Loads and stress state of the large diameter drilled shaft and the pile cap for Incheon Bridge which will be the longest bridge of Korea were investigated through the full modeling for rigid connection conditions.