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Optimization of microwave-assisted extraction process of Hordeum vulgare L. by response surface methodology (반응표면분석법을 이용한 새싹보리 마이크로웨이브 추출공정의 최적화)

  • Lee, Jae-Jun;Park, Dae-Hee;Lee, Won-Young
    • Food Science and Preservation
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    • v.24 no.7
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    • pp.949-956
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    • 2017
  • This study attempted to find optimum extract range of active ingredient for barley sprouts (Hordeum vulgare L.). Extracts from Hordeum vulgare L. were made by microwave extraction method and total polyphenol content (TPC), total flavonoid content (TFC), DPPH radical scavenging activity (DPPH) were measured with extract of Hordeum vulgare L.. Response surface methodology (RSM) was applied to a extraction process, and central composite design (CCD) was also used for this process to examine the optimum condition. Independent variables ($X_n$) are concentration of ethanol ($X_1$: 0, 25, 50, 75, 100%), microwave power ($X_2$: 60, 120, 180, 240, 300 W), extraction time ($X_3$: 4, 8, 12, 16, 20 min). Dependent variables ($Y_n$) are TPC ($Y_1$), TFC ($Y_2$), DPPH radical scavenging ($Y_3$). It is formed by sixteen conditions to extract. The $R^2$ value of dependent variables is ranged from 0.90 to 0.97 (p<0.05). Experiments values within the optimal range (40% of ethanol concentration, 120 W of microwave power, 18 min of extraction time) were 3.74 mg GAE/g (TPC), 3.00 mg RE/g (TFC), 35.43% (DPPH), respectively. Under the optimized conditions, predicted value showed no significant difference comparing with the experimental values.

Synthesis and Cation Binding Properties of Triester Calix[4]arenes and Calix[4]quinones

  • 남계천;강성옥;전종철
    • Bulletin of the Korean Chemical Society
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    • v.18 no.10
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    • pp.1050-1052
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    • 1997
  • The complexes M(CO)4-1,2-(PPh2)2-1,2-C2B10H10 (M=Cr 2a, Mo 2b, W 2c) have been prepared in good yields from readily available bis-diphenylphosphino-o-carboranyl ligand, closo-1,2-(PPh2)2-1,2-C2B10H10 (1), by direct reaction with Group Ⅵ metal carbonyls. The infrared spectra of the complexes indicate that there is an octahedral disposition of chelate bis-diphenylphosphino-o-carboranyl ligand around the metal atom. The crystal structure of 2a was determined by X-ray diffraction. Complex 2a crystallizes in the monoclinic space group P21/n with cell parameters a = 12.2360(7), b = 17.156(1), c = 16.2040(6) Å, V = 3354.1(3) Å3, and Z =4. Of the reflections measured a total of 2514 unique reflections with F2 > 3σ(F2) was used during subsequent structure refinement. Refinement converged to R1 = 0.066 and R2 = 0.071. Structural studies showed that the chromium atom had a slightly distorted pseudo-octahedral configuration about the metal center with two phosphine groups of o-carborane occupying the equatorial plane cis-orientation to each other. These metal carbonyl complexes are rapidly converted to the corresponding metal carbene complexes, [(CO)3M=C(OCH3)(CH3)]-1,2-(PPh2)2-1,2-C2B10H10 (M= Cr 3a, Mo 3b, W 3c), via alkylation with methyllithium followed by O-methylation with CF3SO3CH3.

The Effects of Hospice Volunteer Education Program on Perceptions about Hospice and the Meaning of Life (호스피스 자원봉사자 교육프로그램 참여군과 비 참여군의 호스피스에 대한 인식과 삶의 의미 비교)

  • Kim, Myung-Sook;Lee, Chung-Sook;Kim, Hyung-Chul
    • Asian Oncology Nursing
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    • v.7 no.2
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    • pp.131-139
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    • 2007
  • Purpose: This study was to compare the perceptions about hospice and the meaning of life between the participants and non-participants of the hospice volunteer education program. Method: Descriptive survey research design was used. Participants were 63, and the data collecting period was from October to December, 2006. Instrument developed by Jung-Hee Kim(1990) and Eun-Ja Lee(1998) was modified to measure the perceptions about hospice. To measure the meaning of life, P.I.L (Purpose In Life) instrument by Crumbaugh and Maholick(1969) was utilized. The data were analyzed using $X^2-test$, t-test and Pearson-Correlation Coefficient. Results: 1. The participants in the hospice volunteer education program demonstrated higher perception scores about hospice than the non-participants (t=5.193, p= .001). 2. The program participants also showed higher scores of the meaning of life than non-participants (t=3.084, p=.005). 3. The perception about hospice and the meaning of life had positive correlation (r= .46, p= .01). Conclusion: Therefore, hospice education program must be established in a continual and systematic way in order to standardize the hospice system in Korea.

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The Effects of Prenatal Education on Primiparas' Perception of Delivery Experience, Self-Confidence and Satisfaction in Maternal Role Performance (초산모의 산전교육이 분만경험 지각, 어머니 역할 수행에 대한 자신감, 만족도에 미치는 효과)

  • Oh, Hyun-Ei;Sim, Gung-Sin;Kim, Jin-Sun
    • Women's Health Nursing
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    • v.8 no.2
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    • pp.268-277
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    • 2002
  • A comparative study was conducted to identify the effects of prenatal education on primiparas' self-confidence, satisfaction in maternal role performance and perception of delivery experience. Data were collected from 91 primiparas from August 1 to 30, 2001 using structured questionnaires. The subjects were selected from 2 OB & GY hospitals which operate the same prenatal education programs in M city. One group of subjects was primiparas who did receive prenatal education (n= 44) and another group of subjects was primiparas who did not receive prenatal education (n= 47). This study hypothesized that primiparas in education group would have higher self- confidence, satisfaction in maternal role performance and perception of delivery experience than those of non-education group. Descriptive statistics, $x^2$-test, t-test, and correlation were used for data analyses. The results of this study were as follows: 1. Primiparas in prenatal education group would not have better perception of delivery experience than those of non-education group (t=1.405, p = 0.163). 2. Primiparas in prenatal education group would have higher self-confidence in maternal role performance than those of non-education group (t=7.669, p=0.000). 3. Primiparas in prenatal education group would have higher satisfaction in maternal role performance than those of non- education group (t=4.115, p = 0.000). 4. There was significant correlations between self-confidence and satisfaction (r=0.489, p=0.000). Moreover, there was significant correlation between self-confidence and perception of delivery experience (r = 0.284, p = 0.006). The results of this study indicated that prenatal education needs to be developed and applied to nursing practice to increase self-confidence and satisfaction in maternal role performance. Experimental studies are needed to identify the effect of prenatal education.

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Performance of Rotating Biological Contactor (RBC) under Different Hydraulic Loading Rates and Rotational Speeds on Ammonia Removal in a Recirculating System

  • Son Maeng Hyun;Jeon Im Gi;Jo Jae-Yoon;Moon HaeYoung
    • Fisheries and Aquatic Sciences
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    • v.2 no.1
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    • pp.52-57
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    • 1999
  • Air-drived rotating biological contactor (RBC) system, which is effective method in filtering performance, was tested for the nitrification capacity in a recirculating system. At ammonia concentrations between 0.029 and 0.528 mg/l, the effect of ammonia loading rate on ammonia removal rate at three different hydraulic loading rates could be defined by the following first­order regression models: Hydraulic loading rate of $14.8 m^3/m^3/day:\;y=39.2\times+3.4 (r^2=0.9137)$, Hydraulic loading rate of $26.5 m^3/m^3/day: y=53.3\times+4.0 (r^2=0.8686)$, Hydraulic loading rate of $37.3 m^3/m^3/day: y=58.4\times+4.2 (r^2=0.7755)$, where, $\times$ is ammonia loading rate (mg/l), y is ammonia removal rate $(g/m^3/day)$, The equations showed the optimal ammonia removal rate at the hydraulic loading rate of $26.5m^3/m^3/day$. Below the ammonia concentration of 2.72 mg/l, first-order regression models between ammonia loading rate and ammonia removal rate at three different rates of speed are defined as follows: Rotational speed of $0.75 rpm: y=28.5\times+4.7 (r^2=0.9143)$, Rotational speed of $1.0 rpm: y=33.6\times+8.4 (r^2=0.9534)$, Rotational speed of $2.0 rpm: y=28.9\times+3.6 (r^2=0.9488)$, where, x is ammonia loading rate (mg/l), y is ammonia removal rate $(g/m^3day)$. The equations show the ammonia removal rate at the rotational speed of 1.0 rpm is significantly higher than that at the rotational speed of either 0.75 rpm or 2.0 rpm (P<0.05).

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Testing the Validity and Reliability of FIPS(Face Interval Pain Scale) with Children림s Pain from Intramuscular Injection (환아에게 적용한 얼굴그림 동통척도의 타당도 및 신뢰도 연구)

  • 김주희;김분한
    • Journal of Korean Academy of Nursing
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    • v.24 no.3
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    • pp.461-472
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    • 1994
  • The main purpose of this study was to test the validity and reliability of FIPS as an assessment tool for pain in children. The subject were 81 children whose ages ranged from 3 to 14 years old who were experiencing pain from an intramuscular injection. 40 were being seen in a local primary hospital and 41 in a university hospital. The data were collected in two settings at a 6 month interval, the first was on Nov. 5th 1991 in a local clinic by one doctor, the second was on May 1st. 1992 in a university hospital by two nurse. McGrath's(1985) face interval cards and weight box scale which is a numeral scale that contains from one to five boxes of cards were used as measures. To analyze the subject's ability to use the face scale and weight box scale, statistical frequency was employed. To determine the difference in the rated pain in-tensity on the face interval scale and the weight box, Pearson correlation coefficient and t-test were employed. To compare the difference in the rated pain intensity of the face interval scale and the weight box scale according to subject's general characteristics, X²-test was employed. The findings were as follows : 1. The subject's ages were from 3 to 14 with a mean age of 8.3 years old. There were 54(66.7%) male children and 27(33.3%) female children. 2. The number of subjects who correctly displayed cards ranging from none to severe pain was 66(81.5%) and the number who correctly compared two cards 3 times was 73(90.l%). 3. Correlation coefficients between each level card of the FIPS and WBS (Weight Box Scale) were r=.52∼.80 P<0.01. 4. There was no statistical difference in rating of the intensity on the FIPS and WBS.(t=1.12∼l.02, P<0.22∼0.45). 5. The differences in rating pain intensity according to the children's general characteristics were re-lated to age(X²=8.94, P<0.05), but not to sex (X²=0.23, P=0.80).

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Cloning and Sequencing of a Gene Involved in the Biosynthesis of Exopolysaccharide in Zoogloea Ramigera 115SLR (Zoogloea Ramigera 115SLR로부터 다당류 생합성에 관여하는 유전자의 분리 및 염기서열 결정)

  • Sam-Pin Lee;Min Yoo
    • Biomedical Science Letters
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    • v.6 no.1
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    • pp.1-9
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    • 2000
  • To identity the genes responsible for the biosynthesis of exopolysaccharide, recombinant plasmids pUEX10 and pLEX10 were constructed from plasmid pLEX3 which was isolated from the recombinant cosmid library of Zoogloea ramigera 115. The complete nucleotide sequence of the 1.7 kb genomic DNA insert in plasmid pUEX10 was determined. Its analysis identified two open reading frames (ORF3 & ORF4) which could encode two proteins. The amino acid sequence derived from ORF3 showed the homology with gumC protein in Xanthomonas campestris as well as exoP protein in Rhizobium melizoti. The partial amino acid sequence of ORF4 showed the homology with polysaccharide export protein in Thermotoga maritima. Z. ramigera 115SLR and Z. ramigera 115SLR/pLEX10 showed the similar pattern for EPS production. Yield of exopolysaccharides produced by Z. ramigera 115SLR and Z. ramigera 115SLR/pLEX10 was 0.26% (w/v) and 0.16% (w/v), respectively.

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Adsorption of methylene blue from an aqueous dyeing solution by use of santa barbara amorphous-15 nanostructure: Kinetic and isotherm studies

  • Alizadeh, Reza;Zeidi, Amir
    • Advances in environmental research
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    • v.6 no.2
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    • pp.113-125
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    • 2017
  • Santa Barbara Amorphous-15(SBA-15) nanoparticles were utilized as the inexpensive and effective adsorbents to remove methylene blue dye from the aqueous solution.SBA-15 was created by Zhao et al method. Infrared spectroscopy, X-ray diffraction and scanning electron microscopy (SEM) were used for the evaluated physical properties of SBA-15. The results of diffraction X-ray indicated that was the crystalline structure for it. Also IR spectroscopy indicated was a silica the whole structure of the groups and SEM image verify the structure of relatively identical particles size of SBA-15. Factors affecting adsorption including the amounts of adsorbent, pH and contact time were investigated by a SBA-15 nanomaterial design. The extent of dye removal enhanced with increasing initial dye concentration and pH from 4 to 10. The higher percentage adsorption were obtained under optimum conditions of variables (sorbent dose of 200 mg/liter, initial MB concentration 10 mg/liter, initial pH of 10 and temperature of $25^{\circ}C$). Maximum adsorption happened after the 2 hour and the kinetic processes of the dyes adsorption were described by applying the pseudo-first-order and the pseudo-second-order and the relatively High correlation with the kinetic Ellovich models. It was found that the pseudo-second-order models kinetic equation described the data of dye adsorption with a good correlation (R2>0.999) which indicated chemisorption mechanism. Freundlich and Langmuir adsorption models were investigated in conditions of variables (adsorbent dose 0.01 gr/liter, MB concentration 10, 20, 30 mg/liter, pH of 4, 7, 10, contact time 90 min and temperature of $27^{\circ}C$). The adsorption data were represented by Langmuir isotherm model. These values are higher than the adsorption capacities of some other adsorbents that have recently been published in the literature.

Characterization and Selection of Lactic Acid Bacteria Producing ${\beta}-Galactosidase$ (${\beta}-Galactosidase$ 생산 유산균 선별 및 특성 조사)

  • Lee, Young-Ki;Choi, Susanna;Park, Young-Il;Park, Chan-Sun;Yoon, Byung-Dae;Hwang, Yun-Sik;Kim, Hee-Sik
    • Korean Journal of Microbiology
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    • v.42 no.3
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    • pp.216-222
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    • 2006
  • This study was carried out to select the lactic acid bacteria producing ${\beta}-galactosidase$ (lactase) and investigate the properties of the ${\beta}-galactosidase$. About 100 strains of lactic acid bacteria showing blue colony on the MRS agar medium containing X-gal were isolated from several kinds of Kimchi. Among them, 2 strains were selected as potential ${\beta}-galactosidase$ producers. The selected strains, ET-1 and LA-12, were identified as Lactobacillus fermentum and L. acidophilus, respectively by the analysis of 16S rDNA sequences. They showed relatively high ${\beta}-galactosidase$ activity and cellular viability. Their ${\beta}-galactosidase$ showed the highest activity at $55^{\circ}C$. And the optimum pHs of the enzymes produced by ET-1 and LA-12 were pH 5.5 and pH 7.0, respectively. They were also highly resistant to artificial gastric juice and bile. Two selected strains showed little change of viable cell number for 3 hr incubation in artificial gastric juice, and maintained the viable cell number at $10^8CFU/ml$ for 24 hr in 0.3% oxgall after incubation for 2 hours in artificial gastric juice. Based on these results, ET-1 and LA-12 are expected to be applied in dairy industry.

Changes of Dissolved Oxygen Concentrations During the Transportation of the Olive Flounder (Paralichthys olivaceus) Seedlings (넙치, Paralichthys olivaceus의 종묘수송중 수중 용존산소의 변화)

  • Ko Young-Sik;Chang Young Jin;Kwon Joon-Yeong
    • Journal of Aquaculture
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    • v.8 no.4
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    • pp.251-260
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    • 1995
  • Experimental transportations of the olive flounder (Paralichthys olivaceus) seedlings (body weight $2.1\pm0.39g$) with polyethylene vinyl bag were Performed to study the change of dissolved oxygen (DO) during transportation, survival rate and feeding activity of seedlings after transportation. The distance and time required for the transportations were 272 km and 11 hours, respectively. DO was rapidly decreased within 3 hours (P<0.05) during the transportation of seedlings of 40 fish in 10l of sea water at $15.6\~17.8^{\circ}C$ of initial water temperature with 5 ml/l of initial DO. The decreasing tendency of DO (Y) according to elapsed time (X) was expressed an equation of $Y\;=\;2.7444X^{-0.4780}$ (r=0.8071). Feeding activity of the seedlings after transportation supplemented with oxygen and icepack besides above transport conditions was significantly higher than that of fish supplemented with icepack only (P<0.05). It was desirable to starve the seedlings for 36 hours before transportation. Survival rate of seedlings was greatly reduced, when the dissolved oxygen concentration was lowered below the range of $0.7\~0.94ml/l$ just after transportation.

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