• Korean Journal of Soil Science and Fertilizer
• /
• v.43 no.1
• /
• pp.44-50
• /
• 2010

This study was carried out to analyze physico-chemical properties and to determine stability of the 19 commercially available horticultural substrates in Korea by European standard methods (EN methods). The average pH, EC, T-N, $P_2O_5$ were 5.44, 0.62$dS{\cdot}m^{-1}$, 0.98% and 10.54$mg{\cdot}L^{-1}$, respectively. Dry bulk density and particle density were in the range of 124.47~243.16$kg/m^3$ and 2073.94~2249.99$kg/m^3$, respectively. Seventeen out of 19 horticultural substrates used in this study were 'very stable'. The other2 substrates were classified as 'stable'. Korea's own criteria of stability for the organic materials such as growing substrates and compost need to be developed so that farmers can use the optimum organic materials without anxiety.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2021.06a
• /
• pp.403-403
• /
• 2021

비점오염물질은 강우에 의해 유출되며 계절적, 자연적 편차가 크게 나타나고, 오염원이 광범위하게 산재하고 있어 오염물질의 발생량과 부하량의 관리가 쉽지 않다. 따라서 비점오염원 관리지역은 오염물질이 하천에 미치는 수질 특성을 파악하는 것이 중요하다. 그러나 수질항목만으로 하천의 수질 특성을 파악하기에는 어려움이 있으며, 수질 특성을 파악하기 위해 다양한 분석방법이 필요하다. 본 연구에서는 비점오염원 관리지역 중 골지천 유역의 수질 특성을 파악하기 위해 통계분석 기법을 활용하여 수질항목간의 상관관계를 분석하고자 하였다. 2017년부터 2019년까지 골지천 유역 내 5개 지점에 대하여 비강우시 23회, 강우시 지점별 173회~196회의 모니터링 결과를 이용하였으며, EC, 탁도, BOD₅, SS, T-N, T-P, TOC의 수질항목을 이용하여 Pearson 상관관계 분석을 실시하였다. 상관관계 분석 결과 비강우시 분석 결과 EC는 -0.06~-0.32, T-N은 0.19~0.37로 낮은 상관성을 갖는 것으로 나타났으며, EC와 T-N을 제외한 탁도와 BOD₅, SS, T-P, TOC의 경우 서로 0.6 이상의 상관성을 갖는 것으로 나타났다. 특히, 탁도와 SS, T-P, T-P와 TOC는 0.9 이상의 높은 상관성을 갖는 것으로 나타났다. 강우시 분석 결과 EC는 비강우시와 유사하게 -0.07~-0.24로 낮은 상관성을 갖는 것으로 나타났으며, TOC를 제외한 탁도, BOD₅, SS, T-N, T-P의 경우 서로 0.6 이상의 상관성을 갖는 것으로 나타났다. TOC의 경우 BOD₅, SS와 0.6 이상의 상관성을 갖는 반면 탁도, T-N, T-P와는 0.45, 0.44, 0.57로 다른 항목에 비해 다소 낮은 상관성을 갖는 것으로 나타났다. 비강우시 T-N은 기저 유출에 의한 영향으로 높은 농도를 나타내 다른 항목에 비해 상관성이 낮은 것으로 나타났으며, 강우시에는 비점오염물질의 유입으로 인하여 모든 항목에서 비슷한 상관성을 보이는 것으로 나타났다. 본 연구 결과 탁도와 SS, T-P의 상관성이 비강우시 0.93, 강우시 0.78로 상당히 높은 것으로 나타나, 탁도를 측정하여 SS와 T-P로 환산하여 측정하는 방법에 대한 기초자료로 활용될 수 있을 것으로 판단된다. 그러나 연구 유역의 지형적 특성이 배제되었기 때문에 추후에 이를 반영한 연구가 필요할 것으로 판단된다.

• Korean Journal of Environmental Agriculture
• /
• v.19 no.4
• /
• pp.294-299
• /
• 2000

In order to find out the effects of shading and nitrogen fertilization on the accumulation of $NO_3\;^-$ in leaves of lettuce, lettuce plants were cultivated in the pots under glasshouse condition with different rates of shading(0, 50%) and nitrogen fertilization(100, 180, 200, 300, $400\;kg{\cdot}ha^{-1}$). The pH value was lower in soil after experiment than before experiment, whereas, contents of EC and $NO_3-N$ were higher. As the amounts of nitrogen fertilization were increased, pHs were decreased, but EC and the contents of $NO_3-N$ were increased. At the nitrogen fertilizations of 100, 180, 200, 300 and $400\;kg{\cdot}ha^{-1}$, the germination rates of lettuce were decreased to 84, 78, 76, 72 and 74%, and survival rates were also decreased to 94, 94, 90, 60 and 46%, respectively. However, the fresh weight of lettuce was highest at $45\;g{\cdot}plant^{-1}$ in the recommended fertilizer $plot(180\;kg{\cdot}ha^{-1})$ with non-shading condition. The contents of $NO_3\;^-$ in the leaves of lettuce were increased 2.8-4.1 times under 50% shading conditions than that under non-shading condition. It kept increasing up to seven order of growth phase; however, it started to decrease after eight order phase. Nitrate reductase activity of lettuce in non-shading condition was higher than that in 50% shading condition.

• Journal of Crop Science and Biotechnology
• /
• v.10 no.1
• /
• pp.19-26
• /
• 2007

Glutathione S-transferases(GSTs, EC 2.5.1.18), glyoxalase-I(EC 4.4.1.5) and alliin lyase(alliinase, EC 4.4.1.4) are important enzyme systems in plant bodies. The first two are mainly detoxifying enzymes that utilize glutathione(GSH) in the defense mechanism, and the last one is mainly involved in secondary metabolism and relevant to sulfur compounds derived from GSH. The activities of the three enzymes have been investigated in soluble extracts of vegetable crops, including pumpkin, cabbage, broccoli, radish, carrot, potato, sweet potato, mungbean, and onion. GST activities were detected in all of the vegetables, and the extract of onion bulb exhibited the highest specific activity(648 nmol/min/mgP). The putative GSTs of most of the vegetables were found to be induced by ethanol. The activities of GSTs in onion bulb were found to be markedly inhibited by S-hexyl glutathione and were also inhibited by S-butyl glutathione and S-propyl glutathione. The anti-CmGSTF1 antiserum recognized a thick band for putative onion GST. The estimated glyoxalase-I activity level was also high in onion bulb(4540 nmol/min/mgP), indicating that the thick band detected by Western blot analysis might result from partial recognition of glyoxalase-I by the antiserum. The specific activities for glyoxalase-I were moderate in radish and carrot, and the extracts of other vegetables had rather low levels of activities. The extract of onion also showed the highest specific activity level for alliinase(2069nmol pyruvate/mgP). The extracts of other vegetables also had alliinase activities, although the estimated values were much lower than that of onion.

• Horticultural Science & Technology
• /
• v.19 no.2
• /
• pp.179-185
• /
• 2001

Throughout the world, physical and chemical analyses of horticultural substrates are carried out in many different ways at the different laboratories. In Europe, standardization in properties and analytical methods of horticultural substrates has been a topic over the last decades. As a result, the CEN methods as European standard methods for the physical and chemical analyses were introduced and the final draft was reported in 1999 by CEN(Committee for European Standardization). Dry matter and moisture content are analyzed after drying samples at $103^{\circ}C$. Laboratory compacted bulk density is analyzed by determining the weight of sample compacted in the test cylinder with constant volume. Dry bulk density, particle density, total pore space, water volume, air volume and volume shrinkage are determined by saturating, draining and drying the sample using double rings and a sand suction table. pH and EC are analyzed by 1:5(sample:distilled water) extraction method on the basis of volume. Organic matter and ash content are determined after drying and combusting the samples. Now, CEN methods are being regarded almost as European standard methods. Further study needs to be carried out for universal applicability of the CEN methods to all the substrates.

• Journal of the Mineralogical Society of Korea
• /
• v.17 no.2
• /
• pp.169-176
• /
• 2004

The Osaeg hotspring (long. $128^{\circ}$ 56'E, tat. $37^{\circ}$ 06'N) located in the northester part of Kangwon-do, Korea. The pH value of the hot spring water is 8.31, and the EC, $SO_4$, F and Na are 196 ${\mu}\textrm{s}$/cm, 10.88 mg/L, 8.19 mg/L, and 37.07 mg/L, respectively. The ocherous precipitates are precipitated on the stream bottom of Osaeg hot spring drainage. X-ray diffraction analysis (XRD), scanning electron microscopy (SEM) and stereo-microscope were used to analyze the precipitates. X-ray diffraction analysis shows that the precipitates consist mainly of poorly crystalline materials and messelite. Many spherical and rod-shaped bacteria-like forms were observed in the precipitates.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.30 no.3
• /
• pp.334-339
• /
• 1985

A sweet corn hybrid, Honey Bantam, was planted on 24 May, 1984 in a silty clay loam soil to investigate the effects of polyethylene(P.E.) mulch and different levels and placements of Nitrogen(N) on soil properties and sweet corn growth. A split-split plot design with three replications was employed; P.E. mulch and bare soil were main plot, N levele of 8, 12, 16, and 20 kg/10a were subplot, and band and broadcast of fertilizers were sub-subplots. At early growth stage soil temperature under P.E. mulch was higher than that in bare soil by 5-10$^{\circ}C$, but the differences decreased as plant growth advanced. Soil hardness increased with soil depth while P.E. mulch reduced soil hardness probably by holding high soil moisture. Soil pH decreased up to the 6th week after planting and then increased in bare soil, but it contineously decreased up to the 8th weeks under P.E. mulch regardless N levels and placements. Electrical conductivity(EC) of soil increased up to the 6th weeks after planting and then decreased in all treatments except broadcast of fertilizers under P.E. mulch where EC increased contineously. Generally, soil EC under P.E. mulch was higher than that in broadcast. Broadcast of fertilizers did not affect emergence of seedlings in all N levels under P.E. mulch and bare soil, but band of fertilizers at all N levels under P.E. mulch and higher levels of N in bare soil reduced emergence rate significantly. Percent stand was possitively correlated with soil EC and it strongly influenced the number of marketable ears. Plant growth was enhanced and silking date was earlier by 14-19 days under P.E. mulch compared to bare soil probably due to increased soil moisture, reduced soil hardness and higher soil temperature.

• Microbiology and Biotechnology Letters
• /
• v.36 no.4
• /
• pp.314-319
• /
• 2008

Penicillin G amidase (PGA, benzylpenicillinaminohydrolase, EC 3.5.1.11) is industrially important enzyme which converts penicillin G to 6-aminopenicillanic acid (6-APA) and phenylacetic acid (PAA). The PGA in E. coli ATCC 11105 is secreted into the periplasm after removing signal sequences and becomes heterodimer which composed of two subunits, small subunit (24 kDa) and large subunit (65 kDa). In this study, the PGA gene was obtained from E. coli ATCC 11105 using PCR (polymerase chain reaction) technique. The active PGA was successfully secreated into periplasm in E. coli BL2 1(DE3) harboring pET-pga plasmid. The optimized fed-batch fermentation, consisting of a three-step shift of culture temperature from $37^{\circ}C$ to $22^{\circ}C$, gave a productivity of 19.6 U/mL with a cell growth of 62 O.D. at 600 nm.

• Horticultural Science & Technology
• /
• v.34 no.6
• /
• pp.886-897
• /
• 2016

The physical properties of rooting media for the establishment of plugs in a greenhouse are modified according to variations in the greenhouse environment throughout the season. In this study, we established a standard for rooting media for the production of plug seedlings for each growing season (summer, winter and spring fall). Eight types of peatmoss (PM) and 4 types of perlite (PL) commonly used in Korea were collected and blended with the ratio of 7 parts PM to 3 parts PL (v/v) to make 32 different rooting media blends. We determined the total porosity (TP), container capacity (CC), air-filled porosity (AFP), pH, and electrical conductivity (EC) of the 32 media blends, and 6 media blends were selected for seasonal use. We also conducted additional analyses for plant easily available water (EAW), buffering water (BW), cation exchange capacity (CEC), and nutrient contents in the 6 media blends. The TP, CC, and AFP of the 32 media blends ranged from 64.7 to 96.0%, 42.9 to 90.1%, and 1.3 to 27.8%, respectively, indicating that the physical properties were strongly influenced by the type of PM and PL. The pH and EC of the PMs ranged from 2.96 to 3.81 and 0.08 to $0.47dS{\cdot}m^{-1}$, respectively. However, after blending the PM with the PL the pH was raised and the EC was lowered The media blends selected for the summer growing season were Blonde Golden peatmoss (BG) + No. 1 perlite size < 1 mm (PE1) and Latagro 0-10 mm (L1) + No. 2 perlite size 1-2 mm (PE2). These two media blends had 89.8-90.9% of TP, 80.8-81.3% of CC, and 9.0-9.7% of AFP. The media blends selected for the winter growing season were Sfagnumi Turvas (ST) + PE2 and Latagro 20-40 mm (L3) + PE2. These media blends had 79.9-86.7% of TP, 60.4-74.9% of CC, and 11.8-19.6% of AFP. The TP, CC, and AFP of two media blends, BG + No.3 perlite 2-5 mm (PE3) and Orange peatmoss (O) + PE3, selected for the spring and fall growing seasons, respectively, were 85.2-87.3%, 77.9%, and 7.4-9.4%, respectively. The percentage of EAW of the media blends selected for the spring, summer, and winter growing seasons ranged from 24.2-24.9%, 22.0-28.6%, and 18.0-21.8%, respectively, but the percentages of BW were not significantly different among the selected root media blends. The pH, EC, and CEC of the 6 selected media blends ranged from 3.11-3.97, $0.06-0.26dS{\cdot}m^{-1}$, and $97-119meq{\cdot}100g^{-1}$, respectively.

• Asian Journal of Atmospheric Environment
• /
• v.9 no.1
• /
• pp.66-77
• /
• 2015

In order to survey the seasonal variation of the chemical composition of particulate matter of $2.5{\mu}m$ or less ($PM_{2.5}$), $PM_{2.5}$ was sampled from 8 February 2013 to 31 March 2014 in an industrial area of Chiba Prefecture, Japan. Chemical measurements of the sample included: ionic components ($Na^+$, $NH_4{^+}$, $Ca^{2+}$, $Mg^{2+}$, $K^+$, $Cl^-$, $NO_3{^-}$ and $SO_4{^{2-}}$), carbonaceous components - organic carbon (OC) and elemental carbon (EC), and water-soluble organic carbon (WSOC). Also, secondary organic carbon (SOC) was measured based using the EC tracer method, and char-EC and soot-EC were calculated from the analytical results. The data obtained were interpreted in terms of temporal variation. Of the overall mean value of $PM_{2.5}$ mass concentration obtained during the study period, ionic components, OC and EC accounted for 45.3%, 19.7%, and 8.0%, respectively. $NO_3{^-}$ showed a unique seasonal distribution pattern due to a dependence on temperature and absolute humidity. It was estimated that an approximate temperature of $14^{\circ}C$, and absolute humidity of $7g/m^3$ were critical for the reversible reaction of $NH_4NO_3(p){\leftrightharpoons}NH_3(g)+HNO_3(g)$. The amount of OC and EC contributing to the monthly $PM_{2.5}$ mass concentration was higher in autumn and winter compared to spring and summer. This result could be attributed to the impact of burning biomass, since WSOC and the ratio of char-EC/soot-EC showed a similar pattern during the corresponding period. From the comparison of monthly WSOC/OC values, a maximum ratio of 83% was obtained in August (summer). The WSOC and estimated SOC levels derived from the EC tracer method correlated (R=0.77) in summer. The high occurrence of WSOC during summer was mainly due to the formation of SOC by photochemical reactions. Through long-term observation of $PM_{2.5}$ chemical components, we established that the degree to which the above-mentioned factors influence $PM_{2.5}$ composition, fluctuates with seasonal changes.