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Phylogenetic Analysis of Phyllospadix iwatensis Based on Nucleotide Sequences Encoding 18S rRNA and ITS-1

  • Kim, Jong-Myoung;Choi, Chang-Geun
    • Fisheries and Aquatic Sciences
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    • v.13 no.4
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    • pp.272-277
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    • 2010
  • Seagrasses are marine angiosperms of ecological importance in providing shelter and food to aquatic species as well as maintaining the carbon cycle on earth. Phyllospadix iwatensis is a seagrass of the family Zosteraceae and is distributed along the eastern coast of Korea. The nucleotide sequences of P. iwatensis nuclear genes encoding 18S ribosomal RNA (rRNA) and internal transcribed spacer-1 (ITS-1) were determined for molecular phylogenetic analysis. Genomic DNA was isolated from P. iwatensis and used for PCR amplification of 18S rRNA and ITS-1. Examination of the 18S rRNA sequence of P. iwatensis showed a close (99% similarity) relationship to Zostera noltii, another genus of Zosteraceae, but a distant (84% similarity) evolutionary relationship to other macroalgal Laminariales species. Further discrepancies found in ITS-1 nucleotide sequences between closely related species indicate that the sequence information could be used for species identification.

Taxonomical Classification and Species-specific Detection of Genus Some Phellinus using Phylotype (Phylotype에 의한 수종의 Phellinus속의 분류체계 확립 및 종간구별을 위한 신속동정법 개발)

  • Kim, Cheng-Yun;Lee, Jae-Yun;Kim, Gi-Young;Lee, Ki-Won;Park, Jae-Min;Kim, Mun-Ok;Lee, Tae-Ho;Lee, Jae-Dong
    • The Korean Journal of Mycology
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    • v.31 no.3
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    • pp.121-128
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    • 2003
  • This study was carried out to identify the phylogenetic relationship of Phellinus species and to know its distribution by comparing the DNA sequences of internal transcribed spacer regions(ITS1 and IST2) and 5.8S ribosomal DNA (rDNA) repeat unit. The Phellinus species had their specific sequences in IST1 and 2 regions depending on suedes. The comparison of the ITS sequences of standard strains indicated that the sequences of ITS1 were more variable than those of ITS2. Nine strains of the commercial products of Phellinus species used in this study were identified as P. lintues, P. baumii, P. igniarius, and P. pini. Most of commercial species were P. pini and P. baumii, and P. gilvus was not found. Also, P. linteus was only found in form of mycelial culture rather than fruiting body. Moreover, the species-specific primers were designed based on ITS sequence data. Each species-specific primers were bound in P. lintues(ITSF-PL2R), P. baumii(PB1F-ITS4R), P. igniarius(IF1-IR3), P. pini(PF1-PR3), and P. gilvus(GF2-GR4), respectively. These primer sets would be useful fer the detection of specific-species among unidentified Phellinus species rapidly.

INDEPENDENTLY GENERATED MODULES

  • Kosan, Muhammet Tamer;Ozdin, Tufan
    • Bulletin of the Korean Mathematical Society
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    • v.46 no.5
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    • pp.867-871
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    • 2009
  • A module M over a ring R is said to satisfy (P) if every generating set of M contains an independent generating set. The following results are proved; (1) Let $\tau$ = ($\mathbb{T}_\tau,\;\mathbb{F}_\tau$) be a hereditary torsion theory such that $\mathbb{T}_\tau$ $\neq$ Mod-R. Then every $\tau$-torsionfree R-module satisfies (P) if and only if S = R/$\tau$(R) is a division ring. (2) Let $\mathcal{K}$ be a hereditary pre-torsion class of modules. Then every module in $\mathcal{K}$ satisfies (P) if and only if either $\mathcal{K}$ = {0} or S = R/$Soc_\mathcal{K}$(R) is a division ring, where $Soc_\mathcal{K}$(R) = $\cap${I 4\leq$ $R_R$ : R/I$\in\mathcal{K}$}.

Post Infection Physiobiochemical Alteration at Various Intensities of Leaf spot (Myrothecium roridum) in Mulberry

  • Kumar, P.M.Pratheesh;Qadri, S.M.H.;Pal, S.C.;Mishra, A.K.;Urs, S.Raje
    • International Journal of Industrial Entomology and Biomaterials
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    • v.7 no.2
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    • pp.175-180
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    • 2003
  • Changes in biochemical constituents and physiological alteration were studied in various intensities (1-5%, 6-15%, 16-30%, 31-50% and > 50%) of leaf spot (Myrothecium roridum) on mulberry leaves and compared with healthy leaves. Chlorophyll, total soluble sugar and total protein were decreased (P < 0.01), but total phenol increased due to pathogen infection. Changes in biochemical constituents showed significant correlation with intensity of disease. Chlorophyll ($r^2$= 0.92), and protein (($r^2$= 0.83) possessed negative while phenol (($r^2$= 0.61) possessed positive correlation. Photosynthetic rate, transpiration rate, stomatal conductance, moisture content (%) and physiological water use efficiency (pWUE) were decreased, but stomatal resistance increased in the infected leaves. Physiological parameters also possessed significant (P < 0.01) correlation with disease intensity. Photosynthetic rate (($r^2$= 0.96), transpiration rate ($r^2$=0.88), stomatal conductance (($r^2$= = 0.65), physiological water use efficiency (($r^2$= 0.88) and moisture content (r = 0.85) were negatively but stomatal resistance (($r^2$= 0.75) was positively correlated to disease intensities.

ON 𝜙-SEMIPRIME SUBMODULES

  • Ebrahimpour, Mahdieh;Mirzaee, Fatemeh
    • Journal of the Korean Mathematical Society
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    • v.54 no.4
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    • pp.1099-1108
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    • 2017
  • Let R be a commutative ring with non-zero identity and M be a unitary R-module. Let S(M) be the set of all submodules of M and ${\phi}:S(M){\rightarrow}S(M){\cup}\{{\emptyset}\}$ be a function. We say that a proper submodule P of M is a ${\phi}$-semiprime submodule if $r{\in}R$ and $x{\in}M$ with $r^2x{\in}P{\setminus}{\phi}(P)$ implies that $rx{\in}P$. In this paper, we investigate some properties of this class of sub-modules. Also, some characterizations of ${\phi}$-semiprime submodules are given.

Normal Pairs of Going-down Rings

  • Dobbs, David Earl;Shapiro, Jay Allen
    • Kyungpook Mathematical Journal
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    • v.51 no.1
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    • pp.1-10
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    • 2011
  • Let (R, T) be a normal pair of commutative rings (i.e., R ${\subseteq}$ T is a unita extension of commutative rings, not necessarily integral domains, such that S is integrally closed in T for each ring S such that R ${\subseteq}$ S ${\subseteq}$ T) such that the total quotient ring of R is a von Neumann regular ring. Let P be one of the following ring-theoretic properties: going-down ring, extensionally going-down (EGD) ring, locally divided ring. Then R has P if and only if T has P. An example shows that the "if" part of the assertion fails if P is taken to be the "divided domain" property.

Identification of Bacteria Causing Fermentation of Oriental Melon in Korea (참외 발효과를 유발하는 세균의 동정)

  • Choi, Jae-Eul;Cha, Sun-Kyung;Kim, Jin-Hee;Yuk, Jin-Ah;Hwang, Yong-Soo;Kwon, Soon-Wo
    • Research in Plant Disease
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    • v.9 no.4
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    • pp.189-195
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    • 2003
  • Bacteria causing fermentation in Oriental melon were identified as three independent groups on the basis of 16S rDNA sequence analysis. The 16S rDNA sequence of the strain CM2105 showed the highest identity (99.6%) with that of Microbacterium phyllosphaerae, and also indicated high sequence identity to that of M. holiorum (99.5%). The 16S rDNA sequences of the strain CM2101 and CM2121 matched at the high sequence similarity (98.9%, 98.8, respectively), to that of Pseudomonas pavonacea, and the DNA sequence of CM2126 showed high sequence identity to that of P. costantinii (99.5%), and P. grimontii (99.0%). The 16S rDNA sequence of the strain CM2113 showed the highest identity (99.7%) with that of Enterobacter cloacae. The 16S rDNA sequences, the physiological and biochemical analysis suggested that the strain CM2105 belonged to Microbacterium phyllosphaerae, CM2101, CM2121 and CM2126 to Pseudomonas spp., CM2113 to Enterobacter cloacae.

Impacts of Cropping Systems on the Distribution of Soil Microorganisms in Mid-mountainous Paddy

  • Kang, Ui-Gum;Shin, Woon-Chul;Choi, Jong-Seo;Lee, Yong-Bok;Lee, Young-Han
    • Korean Journal of Soil Science and Fertilizer
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    • v.49 no.5
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    • pp.480-488
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    • 2016
  • Soil microbes are widely well known to play an important role for sustainable agriculture in terms of crop healthy cultivation and environmental conservation. In this context, the distributional characteristics of soil microbes according to cropping systems were investigated under rice (R)-rice (R), rice (R)-barley (B)-rice (R), and soybean (S)-barley (B)-soybean (S) cropping condition to get basic informations for sustainable agriculture, where barley was grown for winter, in mid-mountainous loam paddy located at the altitude of 285 m above sea level in Sangju area from 2014 to 2015. Estimating from microbial communities by fatty acid methyl ester (FAME) method, a total biomass of bacteria, actinomycetes, and fungi in R-B-R plot was 37% and 40% higher than that in S-B-S and R-R plots, respectively (p < 0.05). In especial, bacteria and fungi were more in R-B-R plot than those in any other ones. B. japonicum, AMF, and mesophilic Bacillus sp. were also greater in S-B-S plot than those. In the community distribution, however, bacteria and actinomycetes showed comparatively high values in S-B-S plot relative to either R-R or R-B-R plot including rice, in which fungi outstanding. In the correlation between microbial biomass and soil properties changed by the cropping, bacteria was positively correlated with C:N ratio; actinomycetes with exchangeable Ca; fungi with available $P_2O_5$ (p < 0.05). While these microbes showed negative response to water stable aggregates of soil.

Relationship Between Nursing Organizational Structure and Nursing Outcome (간호 조직구조와 간호결과의 관계)

  • Lee, Eun-Ju
    • Journal of Korean Academy of Nursing Administration
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    • v.10 no.1
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    • pp.37-48
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    • 2004
  • Purpose: This study was investigated to identify the relationship among hospital nursing organizational structure(decentralization, communication, bed/nurse ratio), process(nurses' job satisfaction, nurses' clinical experiences, nurses' unit experiences), and nursing outcome(patient satisfaction, physiologic adaptation, length of stay, number of complication). Method: The subjects consisted of 86 hysterectomy patients and 23 nurses in gynecology unit. Data were collected from May 16, 2002 to August 15. 2003 by the structured questionnaires and chart review. Data analysis was done with ANOVA, Pearson's correlation coefficient, path analysis. Result: Relationship between organizational structure and process ; Bed/nurse ratio was negatively related to nurses' job satisfaction(r=- .37, p<.05), and nurses' clinical experience(r=- .69, p<.00). Decentralization(r=.42, p<.05) and comunication(r=.61, p<.00) were positively related to nurses' clinical experiences. Relationship between process and nursing outcome ; There was a significant relationship between nurses' unit experiences and patient satisfaction(r=.63, p<.00), nurses' job satisfaction and physiologic adaptation(r= .44, p<.05), nurses' unit experiences and physiologic adaptation(r=.64, p<.00), Relationship between organizational structure and nursing outcome ; Decentralization and communication were positively related to patient satisfaction(r=.86, p<.00 ; r=.88, p<.00) and physiologic adaptation(r=,51, p<.01, r=.64, p<.00). Conclusion: Nurses' unit experience, communication, decentralization were significant variables for patient satisfaction. Nurses' unit experience, nurses' job satisfaction, communication were significant variables for patient physiologic adaptation.

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Effects of MicroRNA-106 on Proliferation of Gastric Cancer Cell through Regulating p21 and E2F5

  • Yao, Yong-Liang;Wu, Xiao-Yang;Wu, Jian-Hong;Gu, Tao;Chen, Ling;Gu, Jin-Hua;Liu, Yun;Zhang, Qing-Hui
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.5
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    • pp.2839-2843
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    • 2013
  • Objective: To investigate the effects of miR-106b on malignant characteristics of gastric cancer cells, and explore possible mechanisms. Methods: Expression of miR-106b, p21 and E2F was determined by real-time PCR. Transfection with miR-106b mimics was conducted, and gastric cancer cells with miR-106b overexpression were obtained. Cells transfected with mimic mutants and those without transfection served as negative and blank controls, respectively. Flow cytometry and transwell assays were adopted to detect the effects of miR-106b overexpression on cell cycle, migration and invasion of gastric cancer cells. Results:. The expression of miR- 106b in gastric cancer cells was significantly higher than that in normal gastric mucosa cells. Furthermore, the expression level of miR-106b rose according to the degree of malignacy among the three GC cell strains (MKN- 45 > SGC-7901 > MKN-28). Overexpression of miR-106b shortened the G0/G1 phase and accelerated cell cycle progression, while reducing p21 and E2F5, without any significant effects on the capacity for migration and invasion of gastric cancer cells. Conclusions: miR-106b may promote cell cycling of gastric cancer cells through regulation of p21 and E2F5 target gene expression.