Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of MicroRNA-106 on Proliferation of Gastric Cancer Cell through Regulating p21 and E2F5

  • Yao, Yong-Liang (Kunshan First People's Hospital, Affiliated to Jiangsu University) ;
  • Wu, Xiao-Yang (Kunshan First People's Hospital, Affiliated to Jiangsu University) ;
  • Wu, Jian-Hong (Kunshan First People's Hospital, Affiliated to Jiangsu University) ;
  • Gu, Tao (Kunshan First People's Hospital, Affiliated to Jiangsu University) ;
  • Chen, Ling (Kunshan First People's Hospital, Affiliated to Jiangsu University) ;
  • Gu, Jin-Hua (Kunshan First People's Hospital, Affiliated to Jiangsu University) ;
  • Liu, Yun (Kunshan First People's Hospital, Affiliated to Jiangsu University) ;
  • Zhang, Qing-Hui (Kunshan First People's Hospital, Affiliated to Jiangsu University)
  • Published : 2013.05.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objective: To investigate the effects of miR-106b on malignant characteristics of gastric cancer cells, and explore possible mechanisms. Methods: Expression of miR-106b, p21 and E2F was determined by real-time PCR. Transfection with miR-106b mimics was conducted, and gastric cancer cells with miR-106b overexpression were obtained. Cells transfected with mimic mutants and those without transfection served as negative and blank controls, respectively. Flow cytometry and transwell assays were adopted to detect the effects of miR-106b overexpression on cell cycle, migration and invasion of gastric cancer cells. Results:. The expression of miR- 106b in gastric cancer cells was significantly higher than that in normal gastric mucosa cells. Furthermore, the expression level of miR-106b rose according to the degree of malignacy among the three GC cell strains (MKN- 45 > SGC-7901 > MKN-28). Overexpression of miR-106b shortened the G0/G1 phase and accelerated cell cycle progression, while reducing p21 and E2F5, without any significant effects on the capacity for migration and invasion of gastric cancer cells. Conclusions: miR-106b may promote cell cycling of gastric cancer cells through regulation of p21 and E2F5 target gene expression.

Keywords

References

  1. Anglicheau D, Muthukumar T, Suthanthiran M (2010). MicroRNA: small RNAs with big effects. Transplatation, 90, 105-12.
  2. Carlsbecker A, Lee JY, Roberts CJ, et al (2010). Cell signalling by microRNA165/6 directs gene dose-dependent root cell fate. Nature, 465, 316-21. https://doi.org/10.1038/nature08977
  3. Calin GA, Sevignani C, Dumitru CD, et al (2001). Human microRNA genes are frequently located at fragile sites and genomic regions involved in cancers. Pro Natl Acad Sci USA, 101, 2999-3001.
  4. Chen CF, Ridzon DA, Broomer AJ (2005). Real- time quantification of microRNAs by stem-loop RT- PCR. Nucleic Acids Res, 33, e179. https://doi.org/10.1093/nar/gni178
  5. Chu AS, Friedman JR (2008). A role for microRNA in cystic liver and kidney diseases. J Clin Invest, 118, 3585-7. https://doi.org/10.1172/JCI36870
  6. Ferdin J, Kunej T, Calin GA (2010). Non-coding RNAs: Identification of Cancer-Associated microRNAs by Gene Profiling. Technol Cancer Res Treat, 9, 123-38. https://doi.org/10.1177/153303461000900202
  7. Gong H, Liu CM, Liu DP, et al (2005). The role of small RNAs in human diseases: potential troublemaker and therapeutic tools. Med Res Rev, 25, 361-81. https://doi.org/10.1002/med.20023
  8. Herranz H, Cohen SM (2010). MicroRNAs and gene regulatory networks: managing the impact of noise in biological systems. Genes Dev, 24, 1339-44. https://doi.org/10.1101/gad.1937010
  9. Kahlert C, Kalluri R (2013). Exosomes in tumor microenvironment influence cancer progression and metastasis. J Mol Med, 91, 431-7. https://doi.org/10.1007/s00109-013-1020-6
  10. Konishi H, Ichikawa D, Komatsu S, et al (2012). Detection of gastric cancer-associated microRNAs on microRNA microarray comparing pre- and post-operative plasma. Br J Cancer, 106, 740-7. https://doi.org/10.1038/bjc.2011.588
  11. Li B, Shi XB, Nori D, et al (2011). Down-regulation of microRNA 106b is involved in p21-mediated cell cycle arrest in response to radiation in prostate cancer cells. Prostate, 71, 567-74. https://doi.org/10.1002/pros.21272
  12. Li SC, Liao YL, Ho MR, et al (2012). miRNA arm selection and isomiR distribution in gastric cancer. BMC Genomics, 13, S13.
  13. Livak KJ, Schmittgen TD (2001). Analysis of relative gene expression data using Real-Time quantitative PCR and the 2-rrCt method. Methods, 25, 402-8. https://doi.org/10.1006/meth.2001.1262
  14. Manikandan J, Aarthi JJ, Kumar SD, et al (2008). Oncomirs: The potential role of non-coding microRNAs in understanding cancer. Bioinformation, 2, 330-4. https://doi.org/10.6026/97320630002330
  15. Martin L, Chang HY (2012). Uncovering the role of genomic “dark matter” in human disease. J Clin Invest, 122, 1589-95. https://doi.org/10.1172/JCI60020
  16. Moss EG (2002). MicroRNAs: hidden in the genome. Curr Biol, 12, 138-40. https://doi.org/10.1016/S0960-9822(02)00708-X
  17. Trompeter HI, Abbad H, Iwaniuk KM, et al (2011). MicroRNAs MiR-17, MiR-20a, and MiR-106b act in concert to modulate E2F activity on cell cycle arrest during neuronal lineage differentiation of USSC. PLoS One, 6, e16138. https://doi.org/10.1371/journal.pone.0016138
  18. Xu L, Dai WQ, Xu XF, et al (2012). Effects of multiple-target anti-microRNA antisense oligodeoxyribonucleotides on proliferation and migration of gastric cancer cells. Asian Pac J Cancer Prev, 13, 3203-7. https://doi.org/10.7314/APJCP.2012.13.7.3203
  19. Zeng Y, Wagner EJ, Cullen BR (2002). Both natural and designed microRNAs can inhibit the expression of cognate mRNAs when expressed in human cells. Mol Cell, 9, 1327-33. https://doi.org/10.1016/S1097-2765(02)00541-5
  20. Zhang Y, Liu D, Chen X, et al (2010). Secreted monocytic miR-150 enhances targeted endothelial cell migration. Mol Cell, 39, 133-44. https://doi.org/10.1016/j.molcel.2010.06.010

Cited by

  1. MiR-421 Regulates Apoptosis of BGC-823 Gastric Cancer Cells by Targeting Caspase-3 vol.15, pp.13, 2014, https://doi.org/10.7314/APJCP.2014.15.13.5463
  2. Overexpression of E2F mRNAs Associated with Gastric Cancer Progression Identified by the Transcription Factor and miRNA Co-Regulatory Network Analysis vol.10, pp.2, 2015, https://doi.org/10.1371/journal.pone.0116979
  3. Inhibitory Effects of Phenolic Alkaloids of Menispermum Dauricum on Gastric Cancer in Vivo vol.15, pp.24, 2015, https://doi.org/10.7314/APJCP.2014.15.24.10825
  4. Plasma microRNA might as a potential biomarker for hepatocellular carcinoma and chronic liver disease screening vol.36, pp.9, 2015, https://doi.org/10.1007/s13277-015-3446-7
  5. Circulating MicroRNAs as Biomarkers in Hepatocellular Carcinoma Screening vol.94, pp.10, 2015, https://doi.org/10.1097/MD.0000000000000603
  6. Role of miRNAs and their potential to be useful as diagnostic and prognostic biomarkers in gastric cancer vol.22, pp.35, 2016, https://doi.org/10.3748/wjg.v22.i35.7951
  7. -MicroRNA Pathway Modulates Cell Proliferation in Myoblasts and Skeletal-Muscle Satellite Cells and Promotes Their Commitment to a Myogenic Cell Fate vol.35, pp.17, 2015, https://doi.org/10.1128/MCB.00536-15
  8. Droplet digital PCR-based circulating microRNA detection serve as a promising diagnostic method for gastric cancer vol.18, pp.1, 2018, https://doi.org/10.1186/s12885-018-4601-5