• Journal of Life Science
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• v.14 no.3
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• pp.429-434
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• 2004

Levansucrase gene (levU) from Zymomonas mobilis was subcloned downstream of GALl promoter in pYES 2.0 and pYInu-AT [GALl0 promoter＋exoinulinase signal sequence of Kluyveromyces marxianus], resulting pYES-levU and pYInu-levU, respectively. The two expression plasmids were introduced into an invertase-deficient strain, Saccharomyces cerevisiae SEY2102, and then transformants showing high activity of levansucrase were selected. When each yeast transformants was cultivated in medium containing galactose, the extracellular and intracellular activities of levansucrase reached about 7.17 U/㎖ with the strain harboring pYES-levU and 6.61 U/㎖ with the strain harboring pYInu-levU. It was found that about 50% of levansucrase were detected in the medium and periplasmic space, and exoinulinase signal sequence didn't enhance the secretion efficiency. Furthermore, the recombinant levansucrase expressed in yeast seems to be produced as a hyper-glycosylated form.

• Journal of Korean Foot and Ankle Society
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• v.27 no.2
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• pp.58-66
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• 2023

Purpose: This study aimed to evaluate the effectiveness of deltoid ligament repair on syndesmotic stabilization in patients with acute ankle fractures with ruptured deltoid and syndesmotic ligaments. Materials and Methods: The medical records of 41 patients (41 ankles) who underwent surgery for Weber type B ankle fracture with ruptured deltoid and syndesmotic ligaments were retrospectively analyzed. The mean follow-up duration was 36 months (range 18~65 months). Patients were divided into two groups: those that underwent deltoid ligament repair (the deltoid group) and those who did not (the non-deltoid group). Both groups were also divided into two subgroups, namely, the D1/S1 group, which underwent syndesmotic screw fixation, or the D2/S2 group, which did not. Medial clear space (MCS), tibiofibular clear space (TFCS), anterior fibular line (AFL) ratio, and posterior fibular line (PFL) distance were measured, and visual analog scale (VAS), American Orthopaedic Foot and Ankle Society (AOFAS), and Foot Function Index (FFI) scores were evaluated. Results: TFCS changed significantly after surgery in the D2 and S1 groups (p=0.01, p=0.03, respectively). Subgroup MCSs, TFCSs, and AFL ratios were not significantly altered by surgery in the four subgroups (p=0.82, p=0.45, p=0.25, respectively). However, postoperative PFL distances were significantly different in the D2 and S1 groups and the S1 and S2 groups (p=0.02, p=0.02, respectively). Mean TFCS decreased significantly after surgery in the D2 and S1 groups. The postoperative VAS, AOFAS scores, and FFI were not significantly different between the subgroups (p=0.44, p=0.40, and p=0.46, respectively). Conclusion: Deltoid ligament repair seemed to restore ankle stability without addressing syndesmosis in Weber type B ankle fractures with rupture of deltoid and syndesmotic ligaments.

• Journal of Astronomy and Space Sciences
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• v.30 no.4
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• pp.335-344
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• 2013

TRiplet Ionospheric Observatory-CubeSat for Ion, Neutron, Electron & MAgnetic fields (TRIO-CINEMA) is a CubeSat with 3.14 kg in weight and 3-U ($10{\times}10{\times}30$ cm) in size, jointly developed by Kyung Hee University and UC Berkeley to measure magnetic fields of near Earth space and detect plasma particles. When a satellite is launched into orbit, it encounters ultra-high vacuum and extreme temperature. To verify the operation and survivability of the satellite in such an extreme space environment, experimental tests are conducted on the ground using thermal vacuum chamber. This paper describes the temperature control device and monitoring system suitable for CubeSat test environment using the thermal vacuum chamber of the School of Space Research, Kyung Hee University. To build the chamber, we use a general purpose thermal analysis program and NX 6.0 TMG program. We carry out thermal vacuum tests on the two flight models developed by Kyung Hee University based on the thermal model of the TRIO-CINEMA satellite. It is expected from this experiment that proper operation of the satellite in the space environment will be achieved.

• Journal of Plant Biology
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• v.34 no.4
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• pp.297-302
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• 1991

The effects of polyamines on auxin polar transport were studied in corn coleoptile segments. Among putresine, spermidine and spermine tested in labelled auxin transport, spermidine inhibited auxin polar transport most strongly. Its inhibitory effect appeared after 1 h of transport period. Spermidine inhibited labelled auxin and 14C-benzoic acid accumulation into the tissue in the various pH range tested (pH 4.0-8.0). These results suggest that the inhibition of auxin transport may not be due to decrease in pH by spermine the effect of decreased pH in the extracellular space.

• Communications of the Korean Mathematical Society
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• v.22 no.2
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• pp.209-218
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• 2007

In this paper we investigate the ${\ell}^p$ space structure of the Banach envelope of $WeakL_1$. In particular, the Banach envelope of $WeakL_1$ contains a complemented Banach sublattice that is isometrically isomorphic to the nonseparable Banach lattice ${\ell}^p$, ($1{\leq}p<\infty$) as well as the separable case.

• Bulletin of the Korean Mathematical Society
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• v.43 no.4
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• pp.747-753
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• 2006

We say operators A, B on Hilbert space satisfy Fuglede-Putnam theorem if AX = X B for some X implies $A^*X=XB^*$. We show that if either (1) A is p-hyponormal and $B^*$ is a class y operator or (2) A is a class y operator and $B^*$ is p-hyponormal, then A, B satisfy Fuglede-Putnam theorem.

• The Pure and Applied Mathematics
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• v.23 no.2
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• pp.131-143
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• 2016

In this paper, we present some common fixed point theorems for two pairs of weakly compatible self-mappings on multiplicative metric spaces satisfying a generalized Meir-Keeler type contractive condition. The results obtained in this paper extend, improve and generalize some well known comparable results in literature.

• Communications of the Korean Mathematical Society
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• v.11 no.1
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• pp.153-167
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• 1996

In this paper we investigate the existence of solutions of a nonlinear wave equation $u_{tt} - u_{xx} = p(x, t, u)$$ in $H_0$, where $H_0$ is the Hilbert space spanned by eigenfunctions. If p satisfy condition $(p_1) - (p_3)$, this nonlinear gave equation has at least one solution.

• Kyungpook Mathematical Journal
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• v.59 no.2
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• pp.341-352
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• 2019

Let M be an n-dimensional closed Riemannian manifold with metric g, $d{\mu}=e^{-{\phi}(x)}d{\nu}$ be the weighted measure and ${\Delta}_{p,{\phi}}$ be the weighted p-Laplacian. In this article we will study the evolution and monotonicity for the first nonzero eigenvalue problem of the weighted p-Laplace operator acting on the space of functions along the Yamabe flow on closed Riemannian manifolds. We find the first variation formula of it along the Yamabe flow. We obtain various monotonic quantities and give an example.

• Journal of the Chungcheong Mathematical Society
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• v.34 no.1
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• pp.85-92
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• 2021

In present paper, inspired by the recently paper [1], we give the mixed pressure-velocity regular criteria in view of Lorentz spaces for weak solutions to 3D micropolar equations in a half space. Precisely, if (0.1) ${\frac{P}{(e^{-{\mid}x{\mid}^2}+{\mid}u{\mid})^{\theta}}{\in}L^p(0,T;L^{q,{\infty}}({\mathbb{R}}^3_+))$, p, q < ∞, and (0.2) ${\frac{2}{p}}+{\frac{3}{q}}=2-{\theta}$, 0 ≤ θ ≤ 1, then (u, w) is regular on (0, T].