• Korean Journal of Food Science and Technology
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• v.36 no.3
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• pp.440-447
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• 2004

Characteristics of human ${\beta}-carotene$ 15,15'-dioxygenase isolated by recombinant DNA technology was elucidated. Optimal pH and temperature were 9.0 and $40^{\circ}C$, respectively. Enzyme activity was temperature-sensitive. Enzyme was stable at pH 6.0-9.0 for 24 hr and under $5^{\circ}C$. Half-life of enzyme at $35^{\circ}C$ was 40 min. Crude preparations of enzyme were inhibited by ferrous ion-chelating agent and sulfhydryl-binding agent. GSH offsets inhibitory effect of PCMB. With increasing substrate concentrations, enzyme activity gave typical Michaelis-Menten curve, Based on Hanes-Woolf plot of data, $K_{m}\;and\;V_{max$ were $3.39{\times}10^{6}\;M\;and\;1.2\;pmol/mg$ protein/min, respectively.

• Proceedings of the Korea Water Resources Association Conference
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• 2012.05a
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• pp.126-126
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• 2012

기후변화는 전 세계적으로 다양한 영향을 미치고 있으며 특히, 홍수나 대설로 인한 수문변화에 영향을 준다. 본 연구는 준분포형 연속 모형인 SWAT (Soil and Water Assessment Tool)모형을 이용하여 우리나라 3대 대설지역에 속하는 다목적댐인 충주댐유역(6642.0 m)의 기후변화에 따른 융설이 수문과 수질에 미치는 영향을 분석하고자 한다. 먼저, 융설 모형의 매개변수인 적설분포면적감소곡선 (Snow Cover Depletion Curve; SCDC)을 구축하기 위하여 10년(2000-2010)동안의 Terra MODIS (MODerate resolution Imaging Spectroradiometer) 위성영상자료와 6개 기상관측소(충주, 제천, 원주, 영월, 대관령, 태백)의 최심적설자료를 이용하여 연도별 SCDC을 구축하였다. 구축 결과, 눈이 50% 피복 일 때 snow volume은 연 평균 0.47로 분석되었다. 이를 SWAT 모형에 적용하여 수문과 수질에 대한 적용성 평가를 실시한 결과, 유출의 경우 NSE는 융설기간 동안 평균 0.8, 전체기간은 평균 0.6으로 나타났으며 수질(Sediment, T-N, T-P)의 경우 각각 평균 0.72, 0.70, 0.85을 나타내었다. 미래 기후자료는 IPCC(Intergovernmental Panel on Climate Change)에서 제공하는 SRES(Special Report on Emission Scenarios) A1B, B1 기후변화시나리오의 HadCM3 모델의 결과 값을 이용하였으며 기간은 과거 30년 기후자료(1981-2010, baseline)를 바탕으로 2040s(2020-2059), 2080s(2060-2099)의 두 기간으로 나누어 각각 분석하였으며 기후변화 결과 값의 불확실성을 줄이고자 과거 자료와 GCM의 1981년에서 2000년까지의 값을 비교하여 온도와 강수량의 보정을 실시한 후 LARS-WG를 이용하여 온도와 강수량 자료를 구축하였다. SWAT 모형을 적용한 결과, 평균 1.92 증가한 것으로 나타났으며 유출은 융설기간(Nov-Apr)이 비융설기간(May-Oct)보다 10% 더 증가하였다. 본 연구에서는 SWAT 모형을 통한 유출 및 환경부하량 전망을 목표로 하여 미래 기후변화를 고려한 융설이 다목적댐에서의 유출과 수질 (Sediment, Total Nitrogen, Total Phosphorus)에 미치는 영향을 평가해 보고자 한다.

• Journal of the Korean Wood Science and Technology
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• v.27 no.2
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• pp.15-22
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• 1999

Polymeric meterials that are used for noise and vibration damper in wood/polymer/wood sandwich composites, must have a high loss factor(tan ${\delta}$), and at the same time the storage modulus(E) of $5{\times}10^7$ to $10^9$ dyne/$cm^2$ must withstand over a wide temperature and frequency ranges. In this study, the series of epoxy resinlpolyacrylate interpenetrating polymer networks(IPNs) were synthesized by simultaneous polymerization. Their dynamic tensile properties were measured at 110Hz using Rheovibron instrument. Composite damping factor(tan ${\delta}_c$) and dynamic bending modulus($E_c,\;E_^{\prime\prime}c$) of wood/polymer/wood sandwich composites were measured at 110Hz using a Rheovibron in bending mode of vibration. These dynamic tensile studies indicated that cured epoxy resin/polyacrylates IPNs were semicompatible in the sense that both the shifting of T($E^{\prime\prime}_{max}$) or T(tan ${\delta}_{max}$) and the broadening of glass transition temperature range were observed. Especially, the cured Epikote871/P(n-BMA) IPNs of composition 70/30 to 50/50 showed a relatively high tan a and appropriate E' value over a wide temperature range; consequently the tan a e curves of wood/IPNs/wood sandwich composites was broadened over a wide temperature range.

• Journal of the KSME
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• v.16 no.4
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• pp.41-48
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• 1976

재료의 피로문제에 대해서는 꽤 오래 전부터 많은 연구가 이루어져왔고, 피로의 현상파악에서부 터 피로이론의 구명, 나아가서는 실제문제로서의 피로설계, 피로수명예측 등에 기여한 업적은 아 주 크다 하겠다. 그러나 종래의 피로문제연구의 방향이, S-N 곡선에서 얻어지는 피로한계강도 (더 정확한 표현으론 피로파괴한계강동)에 바탕을 두고, 정력확적인 설계관례인 안전계수의 도입 을 빌려, 피로강도를 실용화할려는 선에서 이루어져 왔다고 보겠다. 재료의 피로한계강도란, 그 정의로 미루어, 다분히 정적으로는 극한강도 또는 피로강도의 개념에 견주어 질 수 있는 공칭응 력으로써 탄성학적으로 해석될 수도 없고, 다만 탄역성이론의 개념을 바탕으로 근사해석례만이 허용되고 있을 뿐이다. 재료에는 소위 평활재이건 절결재이건 간에 또 검출여부에 관계없이, 내외 부에 대소각종의 결함이나 역학적 불연속부가 잠재해있음은 이미 공지의 사실이며, 이들 결합, 불 연속부등이 외하중하에서 응분의 응력집중원이 되어 재료를 전반적인 파괴로 몰고 갈 수 있다 함 도, 또한 이러한 역학적거동이 피로파괴에 까지 확장해석될 수 있을 것이란 것도 이미 잘 알려져 있는 터이라 하겠다. 재료내외부의 제결합을 응력집중이 극대인 crack로 대체해서 외하중하에서 의 응력장거동을 해석한 선형탄성파괴역학(LEFM)은, 바로 이러한 실제재료의 강도설계에 보다 큰 정확성을 부여한 방법론적 학문이라 하겠고, 나아가서는 재료의 파괴기구를 파헤치는데 진일 보적인 역학적인 수법이라 하겠다. 취성파괴, 연성파괴에 바탕을 둔 파괴역학(LEFM)을 피로파괴 에 적용시키는 데는 상당한 문제점들을 수반할 것임은 충분히 인지되나, 제한된 경계조건하에서 의 적용 예는 종래의 어떤 방법에 의한 것 보다도 피로강도설계, 안전사용 피로수명예측 등에 획기적인 진전을 보여주고 있다. 파괴역학은 crack 재의 강도학이고, 더 구체적으로 음력학대계수 (stress intensity factor) K 또는 이와 연연되는 parameter 인 strain energy release rate(G), crack-tip plactic zone size r$_{p}$,.rho., crack-tip opening displacement .phi., strain intensity 등을 쓰는 재료강도학이기 때문에, 이 수법을 피로파괴에 적용시킴은, 종래의 공칭응력으로 피로 문제를 다루던 방법과는 판이하다 하겠다. 본고에선 파괴역학의 관점에서 피로구열의 안정성장을 논하고, 과거 10여년간의 피로 crack문제에 대한 연구방법, 실험방법 등을 소개하는 방향으로 고 를 진행시켜 나가겠다.

• Journal of Nutrition and Health
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• v.50 no.5
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• pp.437-446
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• 2017

Purpose: This meta-analysis aimed to evaluate the effect of cassia cinnamon (Cinnamomum. cassia and C. aromaticum) on the glycemic response with a focus on the preparation of dehydrated powder and water extract. Methods: We searched Pubmed, Cochrane, EMBASE, Science Direct, and the Korean Studies Information Service System (KISS) through May 2017. In the meta-analysis for the preparation of powder, eight trials reporting fasting blood glucose (FBG), four trials reporting HbA1c, and three trials reporting the postprandial glycemic response were included. For the water extract, six trials reporting FBG and four trials reporting HbA1c were eligible for this study. A random-effects model was used to calculate the pooled effect size. Results: Cassia cinnamon powder intake significantly lowered FBG by -1.55 mmol/L (95% CI, -2.45, -0.64; p = 0.001) and the AUC of postprandial blood glucose level by $-51.8mmol/L{\cdot}min$ (95% CI, -85.5, -18.1; p = 0.003). There was a significant difference in FBG between water extract of cinnamon and placebo of -0.76 mmol/L (95% CI, -1.09, -0.43; p = 0.000). However, blood HbA1c level was not significantly altered by any preparation of cinnamon. No statistical heterogeneity was observed for any analysis except in the case of FBG for cinnamon powder. Results of funnel plots and Egger's regression suggest a low likelihood of publication bias in all biomarkers (p > 0.05). Conclusion: According to this meta-analysis, there was possible evidence to support a relationship between cassia cinnamon intake and fasting glucose in both preparation of powder and water extract. Furthermore, new evidence of the health benefits on postprandial glucose regulation of cinnamon powder was obtained.

• Radiation Oncology Journal
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• v.16 no.2
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• pp.99-106
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• 1998

Purpose : We conducted clonogenic assay using human cancer cell lines (MKN-45, PC-14, Y-79, HeLa) to investigate a correlation between the parameters of radiosensitivity. Materials and Methods : Human cancer cell lines were irradiated with single doses of 1, 2, 3, 5, 7 and 10Gy for the study of radiosensitivity and subrethal damage repair capacity was assessed with two fractions of 5Gy separated with a time interval of 0, 1, 2, 3, 4, 6 and 24 hours. Surviving fraction was assessed with clonogenic assay using $Sperman-H\"{a}rbor$ method and mathematical analysis of survival curves was done with linear-quadratic (LQ) , multitarget-single hit(MS) model and mean inactivation dose$(\v{D})$. Results : Surviving fractions at 2Gy(SF2) were variable among the cell lines, ranged from 0.174 to 0.85 The SF2 of Y-79 was lowest and that of PC-14 was highest(p<0.05, t-test). LQ model analysis showed that the values of $\alpha$ for Y-79, MKN-45, HeLa and PC-14 were 0.603, 0.356, 0.275 and 0.102 respectively, and those of $\beta$ were 0.005, 0.016, 0.025 and 0.027 respectively. Fitting to MS model showed that the values of Do for Y-79. MKN-45, HeLa and PC-14 were 1.59. 1.84. 1.88 and 2.52 respectively, and those of n were 0.97, 1.46, 1.52 and 1 69 respectively. The $\v{D}s$ calculated by Gauss-Laguerre method were 1.62, 2.37, 2,01 and 3.95 respectively So the SF2 was significantly correlated with $\alpha$, Do and $\v{D}$. Their Pearson correlation coefficiencics were -0.953 and 0,993. 0.999 respectively(p<0.05). Sublethal damage repair was saturated around 4 hours and recovery ratios (RR) at plateau phase ranged from 2 to 3.79. But RR was not correlated with SF2, ${\alpha}$, ${\beta}$, Do, $\v{D}$. Conclusion : The intrinsic radiosensitivity was very different among the tested human cell lines. Y-79 was the most sensitive and PC-l4 was the least sensitive. SF2 was well correlated with ${\alpha}$, Do, and $\v{D}$. RR was high for MKN-45 and HeLa but had nothing to do with radiosensitivity parameters. These basic parameters can be used as baseline data for various in vitro radiobiological experiments.

• Korean Journal of Environmental Agriculture
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• v.40 no.4
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• pp.313-321
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• 2021

BACKGROUND: Ammonia is a causative substance for the fine particulate matters (PM_2.5) and generates dust through atmospheric reactions. Agricultural sector accounts for 79.3% of ammonia emissions in Korea. Urea and composted organic fertilizer (COF) are used in the soil for the purpose of supplying nutrients in grapevine orchards. This study was conducted to investigate estimates of ammonia emission and examine fruit quality from the rain proof cultivation of the 'Beni Balad', applied by urea and COF to the soil. METHODS AND RESULTS: Urea, COF1, and COF2 were applied at the rates of 119, 135, and 271 kg ha^-1 respectively. Ammonia emissionwas measured using a dynamic flow-through method. CONCLUSION(S): Ammonia emissions by urea and COF treatments to 'Beni Balad' soils under rain proof cultivation were calculated to be 2.63, 12.95, 2.05, and 3.97 kg NH₃-N ha^-1 day^-1, respectively for the control, urea, COF1, and COF2. Urea soil application increased soluble solids, firmness, and anthocyanin content in fruits at harvest, and COF1 application increased the soluble solids and anthocyanin content. For all the treatments, acidity increased in the harvested fruits.

• Solar Energy
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• v.11 no.2
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• pp.51-62
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• 1991

Heat transfer characteristics for heat retrieving processes in a paraffin-filled horizontal circular cylinder was studied. Theoretical and experimental analyses were carried out. In the theoretical analysis, solid and liquid phases were treated separately. Namely, convection for liquid and conduction for solid phase were investigated respectively. The retrieved heat was calculated from the experimentally determined solidified mass. Furthermore, the effects of initial temperature of the liquid and cooling temperature on the heat discharge rate were also studied. In the heat retrieving process, the governing factor for the solidifying rate is the cooling temperature, because most of the liquid sensible heat is rapidly discharged in the initial stage of solidification. Hence heat transfer mechanism during heat retrieving process can be safely considered as conduction. In the cut of frozen paraffin, there showed an empty space in the upper region. It is caused by the temperature drop in the liquid paraffin. While volume shrinkage caused by phase transition was indiscernible. Irrespective of cooling temperature and initial liquid temperature, solidified mass was well-correlated with the product of Fourier number and Stefan number in the solid phase.

• Applied Biological Chemistry
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• v.10
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• pp.15-21
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• 1968

In order to fractionate the rice protein employing paper electrophoresis, 9 subjects of Korean rice and one Indica type, Pin Galw 50 were examined, the results were as follows. 1. Polished rice protein was separated into albumin, globulin, prolamine, and oryzenin. The amount of these fractions was determined by Kjeldahl method showing respectively 0.26%, 0.65%, 0.41%, and 5.01% in average. Albumin was extracted with deionized water, globulin with 10% NaCl, prolamine with 70% ethanol, and oryzenin with 0.05N-NaOH. 2. Albumin was extracted with deionized water and dialyzed by a cellophan tube. The supernatant was submitted to paper electrophoresis using phosphate buffer (pH 7.6, ${\mu}$ 0.18). Albumin was identified as monocomponent in all of 10 varieties under study. Globulin was extracted and dialyzed to remove the albumin. The precipitates were resolved in 10% saline solution and examined by paper electrophoresis. The globulin consists of two components in phosphate buffer(pH 7.6, ${\mu}$ 0.18) Any subject, regardless the origin, appears to contain globulin I and globulin II. Prolamine was extracted with 70% ethanol, dialayzed against deionized water, resolved with ethanol, and analyzed by Paper electrophoresis. It was proved as one component in the 70% alcoholic buffer(pH 9.0, ${\mu}$=0.0095). On the contrary, paper electrophoresis with oryzenin demonstrated two or three components in Sorensen's buffer(pH 13.0, ${\mu}$ 0.11). Yookoo 132, Dungpan 5, Kwansan, and Jaekun contain oryzenin I, oryzenin II, and oryzenin III. On the other hand, Paldal, Jinheung, Sukwang, Eunbangzu, Damakum, and Pin Galw 56 contain only oryzenin II, and oryzenin III. On the basis of these analyses a discussion of the differences between the protein fractions of 10 varieties was presented. 3. Globulin I varied from 0.22% to 0.46% (aver. 0.35%) in the amount, globulin II from 0.21 to 0.44%(aver. 0.32%), oryzenin I from 0.17% to 0.44%(aver. 0.3%), oryzenin II from 1.59% to 2.88%(aver. 2.23%), and oryzenin III from 2.02% to 3.57%(aver. 2.66%).

• Applied Biological Chemistry
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• v.8
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• pp.29-37
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• 1967

Citrus fruits of ten varieties grown in Chaeju island and a few other fruits for the comparison were analyzed to determined the contents of crude fat, crude fiber, total carbohydrates, macroelements and ash. Acids and sugars in fruit juices were also determined and the characteristic of neutralization of fruit juices were investigated. 1. The per cent of edible part of citrus fruits having the range of 48.5(Daingwoochi) to 72(Onju) were lower than that of other fruits. It was lower by about 8 per cent than that of the same variety produced in U.S.A. It was shown that the amount of rind per fruit might be increased in the citrus fruit grown in the low annual temperature. 2. The content of crude protein were around 1% and higher than other's. The contents of crude fat were below 0.1% in three varieties and over 0.1 in others. The contents of crude fiber were between 0.3 to 0.8% and the fruits with the high content of crude protein were inclined to have the high content of crude fiber and it was also shown that the low annual temperature was inclined to increase the amount of crude fiber per fruit. 3. The amount of total acid were from 19.5 m.e per 100 g of fresh fruit in Byongkyul to 44.2 m.e in Daingwoochi. The high percentage of titrable acid was over 90 in two sour varieties, Daingwoochi and Hakyul having pH below 3 and the high content of total acid. These two varieties were above 10 in the ratio of total acid in the edible part to the total acid in the rind(total acid in edible part/total acid in the rind). The content of combined acid was lower than that of titrable acid in the edible part and vice versa in the rind. 4. Navel was highest as 12.82% in the total sugar content and the lowest content was 4.9% of Hakyul. The contents of reducing sugar in the citrus fruits were about half of that in other fruits. The ratio of total sugar to titrable acid (sugar/acid: the grade of sweet taste) were lower than foreign products . 5. From the titration curves of fruit juices the characteristic of neutralization of juices could be grouped in three types, and other values, that is, pH, the content of total acid, the percentage of combined acid, the ratio of total acids in edible part and rind, the content of sugar, and the grade of sweet taste were also devided into the same three categories. 6. The contents of macroelements were different along to the each part of fruit. The content, in the seed were high and the ones in the rind were low. The contents of each element were in the order of $K_2O>N>P_2O_5{\gtrless}CaO{\gtrless}MgO$ in the edible part, $K_2O>N>CaO>P_2O_5{\gtrless}MgO$ in the rind, $N>K_2O>P_2O_5>CaO>MgO$ in the seed. The content of potassium was especially high in Marumeru and Hakyul and the content of calcium in citrus fruit was higher than others.