• Title/Summary/Keyword: P fraction

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Extraction and Component Sugar Analysis of Polysaccharides from Buckwheat (메밀의 다당류 추출과 구성당 분석)

  • Lee, Jung-Sun;Ra, Kyung-Soo;Son, Heung-Soo
    • Korean Journal of Food Science and Technology
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    • v.27 no.6
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    • pp.860-865
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    • 1995
  • This study was conducted to extract and isolate the polysaccharides from buckwheat. Also the sugar composition of the polysaccharides was investigated. The soluble and indigestible polysaccharides were isolated from supernatant and residue after enzyme treatment of raw, roast and steam buckwheat. The yields of low molecular weight soluble polysaccharides(LMS-P: MW<10 Kda) and high molecular weight soluble polysaccharides(HMS-P: MW>10 Kda) were $74.9{\sim}84.2%$ and $5.5{\sim}9.4%$, respectively. The yields of indigestible polysaccharides were low molecular weight insoluble polysaccharides; $0.8{\sim}4.2%$, crude hemicellulose; $3.2{\sim}9.6%$, alcohol insoluble hemicellulose; $0.9{\sim}1.7%$, residue; $2.0{\sim}2.4%$, respectively. The free sugars were detected in the soluble polysaccharides and low molecular weight insoluble polysaccharides but were not detected in the crude hemicellulose, alcohol insoluble hemicellulose and residue. The protein of all fraction were detected and the content was $1.0{\sim}18.9%$. The main sugar of soluble polysaccharides was glucose and the indigestible polysaccharides were composed of glucose, arabinose, rhamnose, xylose, mannose and galactose.

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THE ROLE OF PANAX GINSENG IN DETOXIFICATION OF XENOBIOTICS (독성물질 해독작용에 미치는 인삼의 효능)

  • Lee F.C.;Park J.K.;Kim E.K.;Ko J.K.;Lee J.S.;Kim K.Y.
    • Proceedings of the Ginseng society Conference
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    • 1984.09a
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    • pp.21-26
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    • 1984
  • The balance between metabolic activation of xenobiotics and detoxification of their active metabolites may playa vital role in controlling mutagenic and carcinogenic processes. To assess the possible role of P. ginseng C.A. Meyer in detoxification of xenobiotics, we studied the effects of ginseng on several parameters of the monooxygenasd system, including benzo(a) pyrene monooxygenase(AHH) and benzo(a) pyrene epoxide hydratase(EH) as well as effects of ginseng on the conjugation system. Test animals receiving ginseng saponin-fraction induced epoxide hydratase activity to over $150\%$ (20mg/kg b.w.) of the control and increased glutathione transferase activity (GSH-T) up to $140\%$ (20mg/kg b.w.) of the control, whereas no significant changes were observed in the benzopyrene monooxygenase activity (AHH). Such a selective induction of the inactivation enzyme epoxide hydratase, combined with a marked elevation of the detoxifying enzyme glutathione transferase, without a concurrent induction of benzopyrene monooxygenase which is responsible for the formation of carcinogenic intermediates, demonstrates that ginseng has the potential to alter the metabolic course of carcinogenic polycyclic aromatic hydrocarbons, and thereby enhance detoxification. Thus, ginseng may play an important role in the prevention of tumors caused by carcinogens.

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Separation and Elution Behavior of Some Iron(Ⅲ)porphyrin Complexes by Reversed-Phase Liquid Chromatography (역상 액체 크로마토그래피에 의한 Iron(Ⅲ)porphyrin 착화합물들의 분리 및 용리거동에 관한 연구)

  • Chang Hee Kang;In Whan Kim;Won Lee
    • Journal of the Korean Chemical Society
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    • v.37 no.12
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    • pp.1035-1046
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    • 1993
  • Some iron(III)porphyrin complexes were prepared, and identified by the spectroscopic methods. Elution behavior of iron(III)porphyrin complexes was investigated by reversed-phase HPLC. The optimum conditions for the separation of iron(III)porphyrin complexes were examined with respect to flow rate and mobile phase strength. These complexes were successfully separated on NOVA-PAK $C_{18}$ column using methanol / water(95/5) for $[T_pCF_3PP)Fe(R)]$ and methanol / water (98/2) for $[(P)Fe(C_6F_5)]$ as a mobile phase. It was found that these complexes were largely eluted in an acceptable range of capacity factor value ($0{\leq}logk'{\leq}1$). The dependence of the capacity factor (k') on the volume fraction of water in the binary mobile phase as well as the dependence of k' on the liquid-liquid extraction distribution ratio$(D_c)$ in methanol-water / n-pentadecane extraction system showed a good linearity. It means that the retention of iron(III)porphyrin complexes on NOVA-PAK $C_{18}$ column is largely due to the solvophobic effect. Also, there was a good linear dependence of the capacity factor(k') on the column temperature and enthalpy calculated by van't Hoff plot. From these results, it was confirmed that the retention mechanism of iron(III)porphyrin complexes in reversed-phase liquid chromatography was invariant under the condition of various temperature, and the solvophobic binding process exhibited isoequilibrium behavior.

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Antioxidative, Antimutagenic and Cytotoxic Effects of Functional Food Manufactured from Fermented Soybean Extract (발효 콩을 주원료로 한 기능성 식품 추출물의 항산화성, 항돌연변이원성 및 세포독성 효과)

  • 최승필;오흥석;박종철;남기본;이득식;함승시
    • Food Science and Preservation
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    • v.11 no.2
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    • pp.214-220
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    • 2004
  • This study was carried out to determine the antioxidative, antimutagenic, and anticancer effects of functional food manufactured from fermented soybean(FFMFS) using DPPH free radical donating method, Ames test and cytotoxicity, respectively. FFMFS extracted with ethanol and then further fractionated to n-hexane, chloroform, ethyl acetate(EtOAc), butanol and water, stepwise. Among five fractions, the EtOAc fractions showed highest electron donating activities (31.6 $\mu\textrm{g}$/mL). The inhibition rate of ethanol extract(200 $\mu\textrm{g}$/plate) of FFMFS in the S. typhimurium TA100 strain showed 84.8% against the mutagenesis induced by MNNG. In addition, the suppression of EtOAc fractions with same concentration of FFMFS the S. typhimurium TA98 and TA100 strains showed 88.7% and 92.8% inhibition against Trp-P-l, respectively. The cytotoxic effects of FFMFS against the cell lines with human lung carcinoma(A549), human gastric carcinoma(AGS) and human breast adenocarcinoma(MCF-7) were inhibited with the increase of the extract concentration. The treatment of 1.0 mg/mL FFMFS of EtOAc fraction showed strong cytotoxicities of 84.5%, 88.7% and 85.6% against A549, AGS and MCF-7, respectively.

Chemical Components and Physiological Activities of Young Mulberry(Morus alba) Stem (뽕나무 어린줄기의 화학성분 및 생리활성)

  • 정창호;주옥수;심기환
    • Food Science and Preservation
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    • v.9 no.2
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    • pp.228-233
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    • 2002
  • In order to exploit as a new functional decocted beverage, chemical components, antioxidative and antimicrobial activities young stems of mulberry(Morus alba) were surveyed. The proximate composition was composed of crude fiber 51.12%, ash 13.46%, total sugar 10.38%, crude fat 9.10% and crude protein 5.01%. The P(295.9 mg%) was the highest mineral found in young stem of mulberry and Ca 289.6 mg%, K 209.6 mg%, Na 58.3 mg%, Mg 45.0 mg% and Fe 4.6 mg% in that order. Free sugars was composed of glucose 1.08%, galactose 022%, sucrose 0.20% and fructose 0.16%. Eight fatty acids in stem of mulberry were identified and the major fatty acids were linoleic acid(46.10%), palmitic acid(27.84%) and linolenic acid(10.85%). Among the 17 amino acids detected, total amino acid was 2,450.5 mg% and proline(313.7 mg%) was the most predominant. Methanol extract and ethyl acetate fraction showed stronger activity of the hydrogen donating activities, each of 77.24% and 80.08%, respectively. The methanol extract from young stem of mulberry showed the strongest antimicrobial activities to Bacillus subtitis and Bacitus cereus. Chloroform and ethyl acetate fractions from methanol extract of young stem showed a inhibition zone of 9.0∼19.0 ㎜ in diameter against pathogen bacteria.

Seasonal and Regional Concentrations of Chemical Composition in Rainwater in Daegu Area (대구지역의 빗물 중 화학적 성분의 계절별 및 지역별 농도분포)

  • Song, Hee-Bong;Lee, Eun-Kyeong;Jung, Dong-Sook;Kim, In-Ok;Lee, Myeong-Sug;Kwon, Byoung-Youne;Lee, Kyoung-Chool
    • Journal of Korean Society of Environmental Engineers
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    • v.28 no.5
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    • pp.522-526
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    • 2006
  • To investigate the characteristics of rainwater in Daegu area, parameters such as pH, electrical conductivity, TOC, TIC and TC were analyzed on 34 samples from January to December 2005. Results revealed that higher values were measured in winter than in summer. Furthermore samples that originated from industrial and commercial areas had higher chemical concentrations and conductivity as compared with those from residential and reference areas. Seldom acid-rain occurrence was recorded(27.2%) as compared with non-acid precipitation(72.8%) incidence. Also higher organic carbon fraction(TOC) was detected(72.5%) than inorganic form(TIC)(27.5%) in terms of component ratio with TC. During frequent rainfalls, the concentration of chemicals was decreased to a degree. Conversely, lower rate of rainfall gave out higher chemical values. Thereby the improvement of air qualify in 2005 compare with 2003 report was influenced by the environment authorities' efforts to reduce air pollutions and various factors by the depression of the region's economy.

RHEOLOGIC STUDY ON THE VISCOELASTIC PROPERTIES OF FLOWABLE AND CONDENSABLE RESIN COMPOSITES (유동성 및 응축성 복합레진의 점탄성에 관한 유변학적 연구)

  • Lee, In-Bog;Cho, Byeong-Hoon;Son, Ho-Hyun;Kwon, Hyuk-Choon;Um, Chung-Moon
    • Restorative Dentistry and Endodontics
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    • v.25 no.3
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    • pp.359-370
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    • 2000
  • The purpose of this investigation was to observe the viscoelastic properties of five commercial flowable(Aeliteflo, Flow it, Revolution, Tetric flow, Compoglass flow), three conventional hybrid(Z-100, Z-250, P-60) and two condensable(Synergy compact, SureFil) resin composites. A dynamic oscillatory shear test was done to evaluate the storage shear modulus (G'), loss shear modulus(G"), loss tangent(tan ${\delta}$) and complex viscosity(${\eta}^*$) of the resin composites as a function of frequency - dynamic frequency sweep test from 0.01 to 100 rad/s at $25^{\circ}C$ - by using Advanced Rheometric Expansion System(ARES). To investigate the effect on the viscosity of resin composites of filler volume fraction, the filler weight % and volume % were measured by means of Archimedes' principle using a pyknometer. The results were as follows 1. The complex viscosity ${\eta}^*$ of flowable resins was lower than that of hybrid resins and significant differences were observed between brands. The complex viscosity ${\eta}^*$ of condensable resins was higher than that of hybrid resins. The order of complex viscosity ${\eta}^*$ at ${\omega}$=10 rad/s was as follows, Surefil, Synergy compact, P-60, Z-250, Z-100, Aeliteflo, Tetric flow, Compoglass flow, Flow it, Revolution. The relative complex viscosity of flowable resins compared to Z-100 was 0.04~0.56 but Surefil was 30.4 times higher than that of Z-100. 2. The storage shear modulus G' and the loss shear modulus G" of flowable resins were lower than those of hybrid resins but those of condensable resins were higher. The patterns of the change of loss tangent, tan ${\delta}$, of resin composites with increasing frequency were significantly different between brands. The phase angles, ${\delta}$, ranged from $30.2{\sim}78.1^{\circ}$ at ${\omega}$=10 rad/s. 3. All composite resins represent pseudoplastic nature with increasing shear rate. 4. The complex shear modulus $G^*$ and the phase angle ${\delta}$ was represented by the frequency domain phasor form, $G^*({\omega})=G^*e^{i{\delta}}=G^*{\angle}{\delta}$. The locus of frequency domain phasor plots in a complex plane was a valuable method that represent the viscoelastic properties of composite resins. 5. There was no direct linear correlationship but a weak positive relation was observed between filler volume % or weight % and the viscosity of the resin composites.

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Properties of Hot-water Extracts and Extract-gels of Starches for Mook (묵제조용 전분에서 분리한 열수추출물의 이화학적성질과 추출물겔의 특성)

  • Song, Young-Me;Chung, Koo-Min;Lee, Won-Jong
    • Korean Journal of Food Science and Technology
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    • v.27 no.4
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    • pp.625-630
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    • 1995
  • In order to know the effects of hot-water extracts of starches on gel formation and gel properties, some physicochemical properties and molecular distribution of the extracts were measured. Also, after acid-hydrolysis of starch gels and extract gels, extent of hydrolysis and $\overline{D.P}._n$, and molecular distribution of hydrolysis-residue were measured. Extraction ratio of grain starches (buckwheat, corn, rice, and wheat) were $4.0{\sim}6.6%$. The ratio of acorn and mungbean starches were 11.7 and 13.5%, respectively. Iodine affinity of the extracts was $17.2{\sim}17.7%$ in starches using for Mook, SM(acorn, buckwheat, and mungbean), $13.7{\sim}14.9%$ in starches not using for Mook, SNM(corn, rice, and wheat). Blue value was also higher in SM than in SNM. $\overline{D.P}._n$. of extracts of SM were bigger than those of SNM. In molecular distribution experiment, amylopectin fraction was not found from the extracts except rice starch extract. The extent of acid hydrolysis of the starch gels were $70{\sim}84%$ after 60 days, and the extent of the extract gels were $22{\sim}35%$. The extent of hydrolysis of starch gels and extract gels made with SM were lower than those of SNM.

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Comparison of Pipeline and Clamshell Capping Technologies for the Remediation of Contaminated Marine Sediments (해양 오염퇴적물 정화를 위한 원통관과 클램쉘을 이용한 피복 기술의 비교)

  • Kang, Ku;Hong, Seong-Gu;Kim, Young-Kee;Park, Seong-Jik
    • Journal of Navigation and Port Research
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    • v.41 no.4
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    • pp.195-206
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    • 2017
  • In situ capping technology for marine sediment pollution control has never been applied in South Korea. In this study a pilot project for the capping was carried out in Busan N Harbor. Pipeline and clamshell capping technologies were implemented for the pollution control. Changes of capping shapes, sediment contamination, and the time and costs required for the two constructions were compared. Both the pipeline and clamshell technologies were found to satisfy the target thickness of 50 cm on average. However, the pipeline method did not operate sensitively in terms of change of the sea floor topography, resulting in an uneven shape and a thickness. Organic carbon and ignition loss quite decreased after the pipeline or the clamshell capping while pH showed no significant change. Organic and residual fraction of Cd, Ni, and Zn in the sediments appeared to decrease after all cappings. The pipeline method took a construction time four times as much as the clamshell method. The clamshell method was demonstrated to reduce the construction cost by about 40% compared with the pipeline method. However, a monitoring for all the parameters needs to be conducted at least two years in order to better evaluate an efficiency of the pollution control by these capping constructions.

FORMATION AND EVOLUTION OF SELF-INTERACTING DARK MATTER HALOS

  • AHN KYUNGJIN;SHAPIRO PAUL R.
    • Journal of The Korean Astronomical Society
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    • v.36 no.3
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    • pp.89-95
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    • 2003
  • Observations of dark matter dominated dwarf and low surface brightness disk galaxies favor density profiles with a flat-density core, while cold dark matter (CDM) N-body simulations form halos with central cusps, instead. This apparent discrepancy has motivated a re-examination of the microscopic nature of the dark matter in order to explain the observed halo profiles, including the suggestion that CDM has a non-gravitational self-interaction. We study the formation and evolution of self-interacting dark matter (SIDM) halos. We find analytical, fully cosmological similarity solutions for their dynamics, which take proper account of the collisional interaction of SIDM particles, based on a fluid approximation derived from the Boltzmann equation. The SIDM particles scatter each other elastically, which results in an effective thermal conductivity that heats the halo core and flattens its density profile. These similarity solutions are relevant to galactic and cluster halo formation in the CDM model. We assume that the local density maximum which serves as the progenitor of the halo has an initial mass profile ${\delta}M / M {\propto} M^{-{\epsilon}$, as in the familiar secondary infall model. If $\epsilon$ = 1/6, SIDM halos will evolve self-similarly, with a cold, supersonic infall which is terminated by a strong accretion shock. Different solutions arise for different values of the dimensionless collisionality parameter, $Q {\equiv}{\sigma}p_br_s$, where $\sigma$ is the SIDM particle scattering cross section per unit mass, $p_b$ is the cosmic mean density, and $r_s$ is the shock radius. For all these solutions, a flat-density, isothermal core is present which grows in size as a fixed fraction of $r_s$. We find two different regimes for these solutions: 1) for $Q < Q_{th}({\simeq} 7.35{\times} 10^{-4}$), the core density decreases and core size increases as Q increases; 2) for $Q > Q_{th}$, the core density increases and core size decreases as Q increases. Our similarity solutions are in good agreement with previous results of N-body simulation of SIDM halos, which correspond to the low-Q regime, for which SIDM halo profiles match the observed galactic rotation curves if $Q {\~} [8.4 {\times}10^{-4} - 4.9 {\times} 10^{-2}]Q_{th}$, or ${\sigma}{\~} [0.56 - 5.6] cm^2g{-1}$. These similarity solutions also show that, as $Q {\to}{\infty}$, the central density acquires a singular profile, in agreement with some earlier simulation results which approximated the effects of SIDM collisionality by considering an ordinary fluid without conductivity, i.e. the limit of mean free path ${\lambda}_{mfp}{\to} 0$. The intermediate regime where $Q {\~} [18.6 - 231]Q_{th}$ or ${\sigma}{\~} [1.2{\times}10^4 - 2.7{\times}10^4] cm^2g{-1}$, for which we find flat-density cores comparable to those of the low-Q solutions preferred to make SIDM halos match halo observations, has not previously been identified. Further study of this regime is warranted.