• Biomolecules & Therapeutics
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• 제23권2호
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• pp.141-148
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• 2015

Epithelial mesenchymal transition (EMT) is the first step in metastasis and implicated in the phenotype of cancer stem cells. Therefore, understanding and controlling EMT, are essential to the prevention and cure of metastasis. In the present study, we examined, by Western blot, reverse transcription polymerase chain reaction (RT-PCR), and confocal microscopy, the effects of cardamonin (CDN) on transforming growth factor-${\beta}1$ (TGF-${\beta}1$)-induced EMT of A549 lung adenocarcinoma cell lines. TGF-${\beta}1$ induced expression of N-cadherin and decreased expression of E-cadherin. CDN suppressed N-cadherin expression and restored E-cadherin expression. Further, TGF-${\beta}1$ induced migration and invasion of A549 cancer cells, which was suppressed by CDN. TGF-${\beta}1$ induced c-Jun N-terminal kinase (JNK) activation during EMT, but CDN blocked it. Protein serine/threonine phosphatase 2A (PP2A) expression in A549 cancer cells was reduced by TGF-${\beta}1$ but CDN restored it. The overall data suggested that CDN suppresses TGF-${\beta}1$-induced EMT via PP2A restoration, making it a potential new drug candidate that controls metastasis.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1994년도 춘계학술대회 and 제3회 신약개발 연구발표회
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• pp.260-260
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• 1994

Mammalian pyruvate dehydrogenase complex(PDC) enzyme consists of multiple oopies of three major oligomeric enzymes-El, E2 E3. And protein X is one of the enzymatic constituents which is tightly bound to E2 subunit This complex enzyme is responsible for the oxidative decarboxylation of pyruvate producing of acetyl CoA which is a key intermediate for the entry of carbohydrates into the TCA cycle for its complete metabolic conversion to CO$_2$. And the overall activity of the complex enzyme is regulated via covalent nodification of El subunit by a El specific phosphatase ad kinase. Protein X has lipoyl moiety that undergoes reduction and acetylation during ezymatic reaction and has been known h be involved in the binding of E3 subunit to E2 core and in the regulatory activity of kinase. The purification of protein X has not been achieved majorly because of its tight binding to E2 subunit The E2-protein X subcomplex was obtained by the established methods and the detachment of protein X from E2 was accomplished in the 0.1M borate buffer containing 150mM NaCl. During the storage of the subcomplex in frozen state at -70$^{\circ}C$, the E2 subunit was precipitated and the dissociated protein X was obtained by cntrifegation into the supernatant The verification of protein X was accomplished by (1)the migration on SDS-PAGE, (2)acetylation by 〔2$\^$-l4/C〕 pyruvate, and (3)internal amino acid sequence analysis of tryptic digested enzyme.

• 한국포장학회지
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• 제15권3호
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• pp.93-98
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• 2009

Migration levels of plasticizers, di-n-butyl phthalate (DBP), benzyl-butyl phthalate (BBP), di-n-octyl phthalate(DNOP), di-iso-decyl phthalate (DIDP) and di-iso-nonyl phthalate (DINP), di-(2-ethylhexyl) adipate (DEHA), from 46 poly(vinyl chloride) (PVC) wrap films and 54 PVC gaskets into food simulants were determined using gas chromatography/mass spectrometry (GC/MS). The method was validated with limit of detection (LOD) of $0.01{\sim}0.02\;{\mu}g/mL$ for DBP, BBP, DNOP and DEHA, and $2\;{\mu}g/mL$ for DIDP and DINP. The linearity were found to be > 0.99 for all the compounds in concentration range of $0.1{\sim}81.4\;{\mu}g/mL$, and overall recoveries were ranged from 90.4 ~ 99.6%. DBP, BBP, DNOP, DEHA, DIDP and DINP were not detected in food simulants, except 1 wrap sample from which 0.28 and $0.99\;{\mu}g/mL$ of DEHA were detected respectively when tested with 20% ethanol and n-heptane as food simulants. These values were below the regulatory limitation in European Union (EU).

• International Journal of Industrial Entomology and Biomaterials
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• 제27권1호
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• pp.142-158
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• 2013

The bumblebee, Bombus ignitus (Hymenoptera: Apidae), is a valuable natural resource that is widely utilized for greenhouse pollination in South Korea. Understanding the magnitude of genetic diversity and geographic relationships is of fundamental importance for long term preservation and utilization. As a first step, we sequenced a partial COI gene of mitochondrial DNA (mtDNA) corresponding to the "DNA barcode" region and the complete internal transcribed spacer 2 (ITS2) of nuclear ribosomal DNA from 88 individuals collected in nine South Korean localities. The complete ITS2 sequences were longest among known insects, ranging in size from 2,034 bp ~ 2,052 bp, harboring two duplicated 112-bp long repeats. The 658-bp long mtDNA sequences provided only six haplotypes with a maximum sequence divergence of 0.61% (4 bp), whereas the ITS sequences provided 84 sequence types with a maximum sequence divergence of 1.02% (21 sites). The combination of the current COI data with those of published data suggest that the B. ignitus in South Korea and China are genetically a large group, but those in Japan can be roughly separated into another group. Overall, a very high per generation migration ratio, a very low level of genetic fixation, and no discernable hierarchical population were found to exist among the South Korean populations of B. ignitus, which suggests panmixia. This finding is consistent with our understanding of the dispersal capability of the species.

• 방사성폐기물학회지
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• 제18권spc호
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• pp.75-87
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• 2020

The Korea Atomic Energy Research Institute (KAERI) has developed geological repository systems for the disposal of high-level wastes and spent nuclear fuels (SNFs) in South Korea. The purpose of the most recently developed system, the improved KAERI Reference Disposal System Plus (KRS⁺), is to dispose of all SNFs in Korea with improved disposal area efficiency. In this paper, a system-level safety assessment model for the KRS⁺ is presented with long-term assessment results. A system-level model is used to evaluate the overall performance of the disposal system rather than simulating a single component. Because a repository site in Korea has yet to be selected, a conceptual model is used to describe the proposed disposal system. Some uncertain parameters are incorporated into the model for the future site selection process. These parameters include options for a fractured pathway in a geosphere, parameters for radionuclide migration, and repository design dimensions. Two types of SNF, PULS7 from a pressurized water reactor and Canada Deuterium Uranium from a heavy water reactor, were selected as a reference inventory considering the future cumulative stock of SNFs in Korea. The highest peak radiological dose to a representative public was estimated to be 8.19×10^-4 mSv·yr^-1, primarily from ¹²⁹I. The proposed KRS⁺ design is expected to have a high safety margin that is on the order of two times lower than the dose limit criterion of 0.1 mSv·yr^-1.

• 한국정보과학회논문지:컴퓨팅의 실제 및 레터
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• 제8권2호
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• pp.183-194
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• 2002

이기종 워크스테이션 클러스터링은 응용 프로그램의 병렬 처리에 유용하며 비용 측면에서 효과적이다. 이기종 워크스테이션 클러스터링 환경에서 총작업반환시간을 최소화하기 위해서는 부하 균형 시스템이 필요하다. 기존의 부하 균형 방식은 각 워크스테이션의 처리능력에 가중치를 미리 부여하여 작업을 분배하는 정적방식이거나, 각 워크스테이션의 상대적 처리능력을 얻기 위해서 성능 테스트 프로그램을 수행하는 동적 방식이 있다. 수행되는 응용 프로그램과는 관계없는 성능 테스트 프로그램은 계산시간을 소비하고 총작업반환시간을 지연시킨다. 이 논문은 상대적 부하 색인에 기초한 효과적 작업 분배 방식과 작업 이주 방식을 제안하였으며 이기종 워크스테이션 클러스터 환경에서 부하 균형 시스스템을 설계 구현하였다. 이 논문에서 제안한 방식의 총작업반환시간을 실험을 통하여 부하 균형을 하지 않은 라운드 로빈 방식의 총작업반환시간과 성능 테스트 프로그램에 의한 부하 균형 방식의 총작업반환시간과 비교하였다. 실험 결과는 비교한 방식보다 제안 방식의 결과가 우수함을 보였다.

• 한국지구과학회지
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• 제32권6호
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• pp.654-664
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• 2011

본 연구에서는 석모도 지역의 지열에너지 개발에 중요한 심부 파쇄대 시스템에 대한 보다 광범위하고 정밀한 조사를 위하여 2005-2006년에 수행되었던 44 측점의 탐사 영역을 확대하여 추가로 36 측점에서 자기지전류 및 가청주파수 자기지전류 심부물리탐사 현장 탐사 자료를 획득하였다. 이전의 자료와 함께 모든 자료는 격자상으로 분포하도록 설계하여 관심 영역 내에서 2차원 및 3차원 역산을 통하여 지질 구조 해석을 실시하였다. 역산 해석 결과 지열수가 발견된 시추공 주변으로 파쇄대로 추정되는 최소 2 조의 큰 전기비저항 불연속면을 영상화하였는데, NNE-SWW(북북동-남서) 방향으로 발달한 불연속대는 지열수가 발견된 시추공 방향으로 발달되어있고, 이와 함께 인접한 바다로부터 EW(동-서) 방향으로 불연속면이 발달되어 있음을 볼 수 있었다.

• The Korean Journal of Physiology and Pharmacology
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• 제1권3호
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• pp.285-296
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• 1997

Injury to brain transforms resting astrocytes to their reactive form, the hallmark of which is an increase in glial fibrillary acidic protein (GFAP), the major intermediate filament protein of their cell type. The overall glial response after brain injury is referred to as reactive gliosis. Glial-neuronal interaction is important for neuronal migration, neurite outgrowth and axonal guidance during ontogenic development. Although much attention has been given to glial regulation of neuronal development and regeneration, evidences also suggest a neuronal influence on glial cell differentiation, maturation and function. The aim of the present study was to analyze the effects of glial-hippocampal neuronal co-culture on GFAP expression in the co-cultured astrocytes. The following antibodies were used for double immunostaining chemistry; mouse monoclonal antibodies for confirm neuronal cells, rabbit anti GFAP antibodies for confirm astrocytes. Primary cultured astrocytes showed the typical flat polygonal morphology in culture and expressed strong GFAP and vimentin. Co-cultured hippocampal neurons on astrocytes had phase bright cell body and well branched neurites. About half of co-cultured astrocytes expressed negative or weak GFAP and vimentin. After 2 hour glutamate (0.5 mM) exposure of glial-neuronal co-culture, neuronal cells lost their neurites and most of astrocytes expressed strong CFAE and vimentin. In Western blot analysis, total GFAP and vimentin contents in co-cultured astrocytes were lower than those of primary cultured astrocytes. After glutamate exposure of glial-neuronal co-culture, GFAP and vimentin contents in astrocytes were increased to the level of primary cultured astrocytes. These results suggest that neuronal cell decrease GFAP expression in co-cultured astrocytes and hippocampal neuronal-glial co-culture can be used as a reactive gliosis model in vitro for studying GFAP expression of astrocytes.

• Biomolecules & Therapeutics
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• 제22권3호
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• pp.207-212
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• 2014

Skin hyperpigmentation is one of the most common skin disorders caused by abnormal melanogenesis. The mechanism and key factors at play are not fully understood. Previous reports have indicated that cystamine (CTM) inhibits melanin synthesis, though its molecular mechanism in melanogenesis remains unclear. In the present study, we investigated the effect of CTM on melanin production using ELISA reader and the expression of proteins involved in melanogenesis by Western blotting, and examined the involvement of transglutaminase-2 (Tgase-2) in SK-MEL-2 human melanoma cells by gene silencing. In the results, CTM dose-dependently suppressed melanin production and dendrite extension in a-MSH-induced melanogenesis of SK-MEL-2 human melanoma cells. CTM also suppressed a-MSH-induced chemotactic migration as well as the expressions of melanogenesis factors TRP-1, TRP-2 and MITF in a-MSH-treated SK-MEL-2 cells. Meanwhile, gene silencing of Tgase-2 suppressed dendrite extension and the expressions of TRP-1 and TRP-2 in a-MSH-treated SK-MEL-2 cells. Overall, these findings suggested that CTM suppresses a-MSH-induced melanogenesis via Tgase-2 inhibition and that therefore, Tgase-2 might be a new target in hyperpigmentation disorder therapy.

• 대한지역사회영양학회지
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• 제17권4호
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• pp.407-418
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• 2012

Many displaced North Koreans (NK) are living in South Korea (SK); however nutrition research with the displaced NK is limited. This study examined food and nutrient consumption status of displaced NK children (6-18 year-old) currently living in SK. A total of 154 children were recruited, and a pre-tested dietary behavior questionnaire, food frequency questionnaire, 24-hr recall method were used. Sex- and age-matched SK children (n = 462) randomly selected from 2009-2010 Korea National Health and Nutrition Examination Survey were used as the comparison group. This study found that more NK children skipped breakfast (37%) and dinner (11%), and ate breakfast (38.8%) and dinner (18.2%) without family members than SK children. Many NK children reported that they rarely ate bread, rice cake, hamburger, pizza, fried food, candy. NK children consumed significantly less energy and nutrients (except calcium) and obtained more energy from fat and protein than SK children. Overall index of nutrient quality in NK children, however, was generally good. Length of stay in SK and breakfast skipping rates were significantly associated with lower diet quality. Therefore, nutrition education with displaced NK children should target those who recently came to SK. How to incorporate "new" foods, generally high in energy, sugar, or fat, in healthy ways and importance of breakfast should be emphasized. The growth patterns of the displaced NK children who were born and raised in food-deprived environments and will grow in food-affluent environments of SK should be monitored for health promotion of the NK children and for nutrition policy of the future united Korea.