• Title/Summary/Keyword: Out-of Sequence

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Complete Genome Sequence Analysis of Two Divergent Groups of Sweet potato chlorotic fleck virus Isolates Collected from Korea

  • Kwak, Hae-Ryun;Kim, Jaedeok;Kim, Mikyeong;Seo, Jang-Kyun;Kim, Jeong-Soo;Choi, Hong-Soo
    • The Plant Pathology Journal
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    • 제34권5호
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    • pp.451-457
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    • 2018
  • The Sweet potato chlorotic fleck virus (SPCFV), of the genus Carlavirus (family Betaflexiviridae), was first detected as one of several viruses infecting sweet potatoes (Ipomea batatas L.) in Korea. Out of 154 sweet potato samples collected in 2012 that were showing virus-like symptoms, 47 (31%) were infected with SPCFV, along with other viruses. The complete genome sequences of four SPCFV isolates were determined and analyzed using previously reported genome sequences. The complete genomes were found to contain 9,104-9,108 nucleotides, excluding the poly-A tail, containing six putative open reading frames (ORFs). Further, the SPCFV Korean isolates were divided into two groups (Group I and Group II) by phylogenetic analysis based on the complete nucleotide sequences; Group I and Group II had low nucleotide sequence identities of about 73%. For the first time, we determined the complete genome sequence for the Group II SPCFV isolates. The amino acid sequence identity in coat proteins (CP) between the two groups was over 90%, whereas the amino acid sequence identity in other proteins was less than 80%. In addition, SPCFV Korean isolates had a low amino acid sequence identity (61% CPs and 47% in the nucleotide-binding protein [NaBp] region) to that of Melon yellowing-associated virus (MYaV), a typical Carlavirus.

시계열 데이터베이스에서 DFT-기반 다차원 인덱스를 위한 물리적 데이터베이스 설계 (Physical Database Design for DFT-Based Multidimensional Indexes in Time-Series Databases)

  • 김상욱;김진호;한병일
    • 한국멀티미디어학회논문지
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    • 제7권11호
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    • pp.1505-1514
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    • 2004
  • 시퀀스 매칭은 시계열 데이터베이스로부터 질의 시퀀스와 변화의 추세가 유사한 데이터 시퀀스들을 검색하는 연산이다. 기존의 대부분의 연구에서는 효과적인 시퀀스 매칭을 위하여 다차원 인덱스를 사용하며, 데이터 시퀀스를 이산 푸리에 변환(Discrete Fourier Transform: DFT)한 후, 단순히 앞의 두 개 내지 세 개의 DFT 계수만을 구성 속성 (organizing attributes)으로 사용함으로써 고차원의 경우 발생하는 차원 저주(dimensionality curse) 문제를 해결한다. 본 논문에서는 기존의 단순한 기법이 가지는 성능 상의 문제점들을 지적하고, 이러한 문제점들을 해결하는 최적의 다차원 인덱스 구성 기법을 제안한다. 제안된 기법은 대상이 되는 시계열 데이터베이스의 특성을 사전에 분석함으로써 변별력이 뛰어난 요소들을 다차원 인덱스의 구성 속성으로 선정하며, 비용 모델(cost model)을 기반으로 한 시퀀스 매칭 비용의 추정을 통하여 다차원 인덱스에 참여하는 최적의 구성 속성의 수를 결정한다. 제안된 기법의 우수성을 규명하기 위하여 실험을 통한기존 기법과의 성능 비교를 수행하였다 실험 결과에 의하면, 제안된 기법은 기존의 기법에 비교하여 매우 큰 성능 개선 효과를 가지는 것으로 나타났다.

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Conformational Role of Proline in $\alpha$-Helices

  • Kang, Young-Kee;Kim, Mee-Kyoung
    • 한국생물물리학회:학술대회논문집
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    • 한국생물물리학회 1998년도 학술발표회
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    • pp.22-22
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    • 1998
  • Conformational energy calculations have been carried out for proline-containing alanine-based pentadecapeptides with the sequence Ac-(Ala)/sun n/-Pro-(Ala)$_{m}$-NHMe (n + m = 14), in order to figure out the positional preference of proline in $\alpha$-helices. The conformational energy was computed a sum of the potential energy (ECEPP/3) and the hydration free energy computed by the hydration shell model.(omitted)d)

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바닥하중 및 면내압축력을 받는 플렛 플에이트 슬래브 (Reinforced Concrete Flat plates Under Combined In-plane and Out-of-plane Loads)

  • 박홍근;김의회;홍성걸
    • 한국콘크리트학회:학술대회논문집
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    • 한국콘크리트학회 1998년도 가을 학술발표논문집(II)
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    • pp.424-429
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    • 1998
  • Numerical studies are performed to investigate the behavior of flat plates under combined in-plane and out-of-plane loads. The numerical model is verified by comparison with experiments for plates simply supported on four edges. Through study on different load combination and loading sequence, the critical load condition that governs the strength of the flat plate is determined. Parametric studies are performed to investigate the buckling coefficient and the effective flexural rigidity so that the moment magnification method is applicable to the flat plates.

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Seismic performance assessments of precast energy dissipation shear wall structures under earthquake sequence excitations

  • Zhang, Hao;Li, Chao;Wang, Zhi-Fang;Zhang, Cai-Yan
    • Earthquakes and Structures
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    • 제18권2호
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    • pp.147-162
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    • 2020
  • This paper presents a novel precast energy dissipation shear wall (PEDSW) structure system that using mild steel dampers as dry connectors at the vertical joints to connect adjacent wall panels. Analytical studies are systematically conducted to investigate the seismic performance of the proposed PEDSW under sequence-type ground motions. During earthquake events, earthquake sequences have the potential to cause severe damage to structures and threaten life safety. To date, the damage probability of engineering structures under earthquake sequence has not been included in structural design codes. In this study, numerical simulations on single-story PEDSW are carried out to validate the feasibility and reliability of using mild steel dampers to connect the precast shear walls. The seismic responses of the PEDSW and cast-in-place shear wall (CIPSW) are comparatively studied based on nonlinear time-history analyses, and the effectiveness of the proposed high-rise PEDSW is demonstrated. Next, the foreshock-mainshock-aftershock type earthquake sequences are constructed, and the seismic response and fragility curves of the PEDSW under single mainshock and earthquake sequences are analyzed and compared. Finally, the fragility analysis of PEDSW structure under earthquake sequences is performed. The influences of scaling factor of the aftershocks (foreshocks) to the mainshocks on the fragility of the PEDSW structure under different damage states are investigated. The numerical results reveal that neglecting the effect of earthquake sequence can lead to underestimated seismic responses and fragilities, which may result in unsafe design schemes of PEDSW structures.

고려인삼(Panax ginseng C.A. Meyer) ATPase $\alpha$-subunit 유전자의 Cloning (Molecular Cloning of ATPase $\alpha$-Subunit Gene from Mitochondria of Korean Ginseng (Panu ginseng C.A. Meyer))

  • Park, Ui-Sun;Choi, Kwan-Sam;Kim, Kab-Sig;Kim, Nam-Won;Choi, Kwang-Tae
    • Journal of Ginseng Research
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    • 제19권1호
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    • pp.56-61
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    • 1995
  • Molecular cloning and restriction mapping on ATPase $\alpha$-subunit gene (atpA) were carried out to obtain genomic information concerned with the gene structure and organization in Korean ginseng mitochondria. Two different clones containing the homologous sequence of atpA gene were selected from SalI and PstI libraries of mitochondrial DNA (mtDNA) of Korean ginseng. The sizes of mtDNA fragments inserted in SalI and PstI clones were 3.4 kb and 13 kb, respectively. Southern blot analysis with [$^{32}P$] labelled Oenothera atPA gene probe showed that atpA gene sequence was located in 2.0 kb XkaI fragment in PstI clone and in 1.7 kb XbaI fragment in SalI clone. A partial sequening ascertained that the SalI clone included about 1.2 kb fragment from SalI restriction site to C-terminal sequence of this gene but about 0.3 kb N-terminal sequence of open reading frame was abscent. The PstI fragment was enough large to cover the full sequence of atpA gene. The same restriction pattern of the overlapped region suggests that both clones include the same fragment of atiA locus. Data of Southern blot analysis and partial nucleotide sequencing suggested that mtDNA of Korean ginseng has a single copy of atpA gene. Key words ATPase a-subunit, mitochondrial DNA, Panax ginseng.

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OFDM 수신기의 CORDIC 기반 주파수 동기를 위한 선형적인 위상 표현 방법 (Phase Representation with Linearity for CORDIC based Frequency Synchronization in OFDM Receivers)

  • 김시현
    • 대한전자공학회논문지SP
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    • 제47권3호
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    • pp.81-86
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    • 2010
  • CORDIC (COordinate Rotation DIgital Computer) 은 간단한 하드웨어로 벡터의 위상으로의 변환이나 회전 등의 위상 연산을 할 수 있으므로 OFDM 수신 시스템에서의 주파수 동기부를 설계할 때 효과적으로 사용될 수 있다. 그러나 CORDIC 알고리듬에서 위상을 표현하는 방향 시퀀스 (direction sequence, DS) 가 선형적이지 않기 때문에 사용상의 많은 제약이 존재한다. 본 논문에서는 근사적 선형성을 지닌 LBDS (linearized binary direction sequence) 표현 방법을 제안하고, LBDS의 최대 위상오차에 대해 분석한다. 또한 DS로부터 LBDS로 변환하는 하드웨어와 그 역변환 하드웨어의 구조를 제안한다. LBDS를 채택하면 위상 추정, 주파수 오차 루프 필터링, 위상 보정 역회전 등 주파수 동기의 전 과정에 CORDIC과 일반적인 산술 연산기를 사용할 수 있다. T-DMB 복조기에 사용될 수 있는 22비트 LBDS에 대한 예도 기술된다.

rDNA-ITS 및 CAPS 분석에 의한 꽃송이버섯 (Sparassis crispa) 수집균주의 계통분류학적 특성구분 (Phylogenetic relationships of medicinal mushroom Sparassis crispa strains using the rDNA-ITS and CAPS analysis)

  • 정종천;이명철;전창성;이찬중;신평균
    • 한국버섯학회지
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    • 제8권1호
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    • pp.27-32
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    • 2010
  • 본 시험은 국내외에서 수집한 꽃송이버섯균 22균주에 대하여 분자생물학적 유연관계를 분석하고자 하였다. 수집균주의 ribosomal DNA의 ITS 영역에 대한 cleaved amplified polymorphic sequence (CAPS) 분석 결과, KACC50866은 다른 균주들과 20%이하의 유연관계를 나타내었으며 나머지 균주들은 90% 이상의 유연관계를 보이면서 4그룹으로 구분되었다. 따라서 이들의 세분화된 분자생물학적 구분을 위하여 rDNA ITS 영역의 염기서열분석을 하여 구분하여 본 결과 KACC50866 균주는 다른 꽃송이버섯균과 유연관계가 매우 낮은 것으로 나타났다. 그리고 나머지 21개 균주는 같은 그룹으로 구분되어 있어 같은 종으로 생각할 수 있으나, 이들을 좀더 세분하기 위해서는 미토콘드리아의 유전자 서열 분석 등이 병행되어야 할 것으로 판단된다.

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Partial Sequence of the Bovine (Bos taurus coreanae) Myogenic Factor Encoding Gene MyoD

  • Kim, H.S.;Park, E.W.;Yoon, D.H.;Kim, H.B.;Cheong, I.C.;Cho, B.W.;Im, K.S.
    • Asian-Australasian Journal of Animal Sciences
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    • 제12권5호
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    • pp.689-694
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    • 1999
  • This experiment was carried out to isolate the partial bovine (Bos Taurus coreanae) myogenic factor encoding gene, MyoD, using the rat myogenic factor (MyoD) gene sequence and to compare the gene sequence between another myogenic factor (Myf 5) and MyoD gene of the bovine. To make the probe and isolate the MyoD gene, PCR was performed to amplify rat and bovine MyoD gene including exon I, II and intron I. The homology between mouse and bovine MyoD is high; bovine MyoD gene shows 17 different gene sequence region compared to rat MyoD. Among those, two regions have significant differences; one is the exon I part between 2834 and 2850 bp, the other is intron part between 3274 and 3303 bp of the mouse. At this region homology was 40% in the former and 50% in the latter. Homology between bovine MyoD and Myf5 was 83% in the exon 1. Especially exon I in the Myf5 602-617 bp and 651-683 bp have significant differences. These results suggest that MyoD gene have a similar gene structure in mouse and bovine and MyoD and Myf5 of the bovine, at least in part, have a similar expression and activity.

Reliability of Stereotactic Coordinates of 1.5-Tesla and 3-Tesla MRI in Radiosurgery and Functional Neurosurgery

  • Kim, Hae Yu;Lee, Sun-Il;Jin, Seong Jin;Jin, Sung-Chul;Kim, Jung Soo;Jeon, Kyoung Dong
    • Journal of Korean Neurosurgical Society
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    • 제55권3호
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    • pp.136-141
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    • 2014
  • Objective : The aims of this study are to identify interpersonal differences in defining coordinates and to figure out the degree of distortion of the MRI and compare the accuracy between CT, 1.5-tesla (T) and 3.0T MRI. Methods : We compared coordinates in the CT images defined by 2 neurosurgeons. We also calculated the errors of 1.5T MRI and those of 3.0T. We compared the errors of the 1.5T with those of the 3.0T. In addition, we compared the errors in each sequence and in each axis. Results : The mean difference in the CT images between the two neurosurgeons was $0.48{\pm}0.22mm$. The mean errors of the 1.5T were $1.55{\pm}0.48mm$ (T1), $0.75{\pm}0.38$ (T2), and $1.07{\pm}0.57$ (FLAIR) and those of the 3.0T were $2.35{\pm}0.53$ (T1), $2.18{\pm}0.76$ (T2), and $2.16{\pm}0.77$ (FLAIR). The smallest mean errors out of all the axes were in the x axis : 0.28-0.34 (1.5T) and 0.31-0.52 (3.0T). The smallest errors out of all the MRI sequences were in the T2 : 0.29-0.58 (1.5T) and 0.31-1.85 (3.0T). Conclusion : There was no interpersonal difference in running the Gamma $Plan^{(R)}$ to define coordinates. The errors of the 3.0T were greater than those of the 1.5T, and these errors were not of an acceptable level. The x coordinate error was the smallest and the z coordinate error was the greatest regardless of the MRI sequence. The T2 sequence was the most accurate sequence.