• Tuberculosis and Respiratory Diseases
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• v.46 no.3
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• pp.363-371
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• 1999

Background: Because of the widespread use and availability of agricultural insecticides, acute organophosphate poisoning as a suicide or an accident is becoming the most common type of poisoning and serious problem in Korea. The mortality of organophosphate poisoning varied from 10 to 86 percent. The cause of death was thought to be a combination of excessive bronchial secretion, bronchospasm, respiratory muscle paralysis and depression of respiratory center, summarily respiratory failure. We evaluated the respiratory complications in patients with acute organophosphate intoxication to determine the predisposing, factors to respiratory failure and to reduce the incidence of respiratory failure or mortality. Method: We conducted a retrospective study of 111 patients with the discharge diagnosis of organophosphate poisoning who were hospitalized at Yenugnam University Hospital during the 5 years. The diagnosis of organophosphate poisoning has based on the followings (1) a history of exposure to an organophosphate compounds. (2) the characteristic clinical signs and symptoms. (3) decrease in the cholinesterase activity in the serum. Results: Respiratory failure developed in 31(28%) of 111 patients with acute organophosphate poisoning. All cases of respiratory failure developed within 96 hours after poisoning and within 24 hours in 23 patients. The 80 patients who did not develop respiratory failure survived. In 31 patients with respiratory failure, 15(44%) patients were dead. The patients with respiratory failure had more severe poisoning, that is, the lower level of serum cholinesterase activity on arrival, the higher mean dosage of atropine administered within first 24 hours. In 16 patients with pneumonia, 14 patients developed respiratory failure. In 5 patients with cardiovascular collapse, 2 patients developed respiratory failure. There was no correlation to between age, sex, the use of pralidoxime and respiratory failure. The serum cholinesterase level in survivors at time of respiratory failure and weaning was $66.05{\pm}85.48U/L$, $441{\pm}167.49U/L$, respectively. Conclusion: All the respiratory failure complications of acute organophosphate poisoning occurred during the first 96 hours after exposure. The severity of poisoning and pneumonia were the predisposing factors to respiratory failure. Aggressive treatment and prevention of the above factors will reduce the incidence of respiratory failure.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1998.11a
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• pp.108-113
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• 1998

Egasyn is accessory protein of ${\beta}$-glucuronidase(${\beta}$-G) in the liver microsomes. Liver microsomal ${\beta}$-G is stabilized within the luminal site of the microsomal vesicles by complexation with egasyn which is one of carboxylesterase isozymes. We investigated the effects of organophosphorus compounds(OPs) such as insecticides on the dissociation of egasyn-${\beta}$-glucuronidase(EG) complex. The EG complex was easily dissociated by administration of OPs, i.e., Fenitrothion, EPN, Phenthionate, and bis-p-nitrophenyl phosphate(BNPP), and resulting ${\beta}$-G dissociated was released into blood, leading to the rapid and transient increase of plasma ${\beta}$-G level with a concomitant decrease of liver microsomal ${\beta}$-G level. In a case of phenthionate treatment, less increase in plasma ${\beta}$-G level was observed, as compared with those of other OPs. This may be explained by a fact that phenthionate was easily hydrolyzed by carboxylesterase. Similarly, carbamate insecticides such as Carbaryl caused rapid increase of plasma ${\beta}$-G level. In contrast, no significant increase of plasma ${\beta}$-G level was observed when pyrethroid insecticides were administered to rats. This is due to a fact that pyrethroids such as Phenthrin and Allethrin were easily hydrolyzed by A-esterase as well as carboxylesterase. On the other hand, addition of OPs to the incubation mixture containing liver microsomes caused the release of ${\beta}$-G from microsomes to the medium. From these in vivo and in vitro data, it is concluded that increase of the plasma ${\beta}$-G level after OPs administration is much more sensitive biomarker than cholinesterase inhibition to acute intoxication of OPs and carbamates.

• Journal of Life Science
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• v.10 no.2
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• pp.164-168
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• 2000

To understand the biological characteristics of Corythucha ciliata occurring in Korea, developmental periods and its susceptibility to several insecticides were examined under growth chamber condition at $25^{\circ}C$. It took 11.1 day from egg to hatch. And duration of each stage up to the 5th nymph after hatching was 4.0, 2.1, 2.0, 2.9, and 4.0 days, respectively. Total duration from egg to adult was 26.1 days, preoviposition period was 12.2 days, and average number of eggs laid by a female was 83.0. Sex ratio of female was 51.6% and lifespan of female adult was 43.2% days. LC50 value of deltamethrin and esfenvalerate were 0.8 and 0.9 ppm, respectively. Insecticidal effects were better in pyrethroids than organophosphates and carbamates.

• Korean journal of applied entomology
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• v.26 no.2 s.71
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• pp.83-88
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• 1987

Differences in susceptibility of several organophosphate, carbamate and pyrethroid insecticides to three species of aphids were determined by leaf-dipping and spray methods. The insects tested were the apterous adults of the green peach aphid (Myzus persicae), cotton aphid (Aphis gossypii) and soybean aphid (Aphis glycines). The comparison of $LC_{50}$ levels was indicated as follows; (1) The susceptibility to insecticides tested were greatly varied with the test methods. (2) The leaf dipping method is more recommendable than the spray method for insecticide screening with aphids. It was easier, more accurate and less variable than the latter. (3) The susceptibility to insecticides was greatly varied between the aphid species. Soybean aphid was more susceptible to the insecticides than green peach aphid and cotton aphid. Exceptionally, pirimicarb was not effective on the cotton aphid.

• The Korean Journal of Pesticide Science
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• v.11 no.3
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• pp.171-178
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• 2007

Esterase isozymes were extracted from final instar larvae of M. saltuarius treated with selective diets and inhibitors. Twenty esterase isozymes were separated on 12% native-PAGE gel and stained with three different substrates(${\alpha}$-naphthyl acetate, ${\beta}$-naphthyl acetate, or ${\alpha}$-naphthyl butyrate). Interestingly, the isozymes of Est7(${\alpha}$-naphthyl acetate and ${\alpha}$-naphthyl butyrate) and Est6(${\beta}$-naphthyl acetate) were specifically activated in final instar larvae fed with the bark of Pinus koraiensis. However, we could not find any band from substrate ${\beta}$-naphthyl stearate. The esterase activities of Est3, Est6, and Est7 were inhibited by organophosphate and carbamate insecticides. In addition, The esterase activities of Est4, Est6, and Est7 were also inhibited by eserine. However, inhibition of esterase activities in methoprene, bornyl acetate, linal, cineol, and citral was not observed. However, It is necessary to reconfirm these results in vivo.

• Journal of The Korean Society of Clinical Toxicology
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• v.12 no.2
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• pp.77-84
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• 2014

Purpose: Carbamate insecticides are potent cholinesterase inhibitors capable of causing severe cholinergic toxicity. Use of carbamate rather than organophosphate insecticides has been increasing. Compared with organophosphate poisoning, relatively few studies have investigated carbamate-associated acute pancreatitis. We investigated general characteristics and pancreatitis of carbamate poisoning and the predictors, among those readily assessed in the emergency department. Methods: We performed a retrospective review of consecutive patients, aged over 18 years, who were admitted between January 2008 and April 2012 to an emergency department (ED) of an academic tertiary care center for treatment of carbamate poisoning. Patients who exhibited poisoning by any other material, except alcohol, were excluded. After application of exclusion criteria, patients were divided according to carbamate-induced pancreatitis and non-pancreatitis groups. Results: A total of 41 patients were included in this study. Among these 41 patients, the prevalence of acute pancreatitis was 36.6% (15 patients). Initial blood chemistry tests showed a statistically higher glucose level in the pancreatitis group, compared with the non-pancreatitis group (222, IQR 189-284 vs. 137, IQR 122-175 mg/dL, P<0.05). Regarding clinical courses and outcomes, a significantly higher proportion of patients developed pneumonia [10 (66.7%) vs. 6 (23.1%), P<0.05] and had a longer hospital stay (7 days, IQR 6-12 vs. 5 days, IQR 2-11, P<0.05), but no difference in mortality, in the pancreatitis group vs. the non-pancreatitis group. In multivariate analysis, the initial glucose was showing significant association with the presentation of carbamate-induced acute pancreatitis (odds ratio 1.018, 95% confidence interval 1.001-1.035, P<0.05). Conclusion: Carbamate-induced acute pancreatitis is common, but not fatal. Initial serum glucose level is associated with acute pancreatitis.

• Korean journal of applied entomology
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• v.14 no.2 s.23
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• pp.65-70
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• 1975

The study was planned to detect resistance levels of striped rice borers (Chilo suppressalis Walker) and green rice leafhoppers(Nephotettix cincticeps Uhler) from different localities to organophosphate and carbamate insecticides using topical application. Strains of overwintering striped rice borers were collected from 7 different areas in Gyeonggi Province and they were kept under conditions of about $25^{\circ}C$ for 10 days. Overwintered and reared larvae of striped rice borers were compared for response to insecticides. The strains of green rice leafhopper were collected from Iri, Milyang, Pyongtaek, Echeon and Suweon. Insects were multiplied in the lab. All insecticides tested were organphosphates(MPP, MEP and Diazinon) and carbamate (NAC), which have been used in control of rice insect pests for over past 10 years. The results obtained were as follows; a. With MPP compound, resistant levels of the Joam, Suweon, and Echeon strains of C. suppressalis were from 2 to 4 times as compared with the Yangpyong strain. b. With MEP insecticide, resistant levels of the Kimpo, Pyongtaek, Yongin, Echeon, Suweon, and Joam strains of C. suppressalis were from 2 to 6 times as compared with the Yangpyong strain. c. With Diazinon, resistant levels of the Suweon strain of C. suppressalis were 3 times greater than the Yangpyong strain; but the resistant levels of Kimpo, Pyongtaek, Yongin, and Echeon strains approximated the later. 4. The overwintered larvae of C. suppressalis were more tolerant to the insecticides than the larvae reared in the lab. e. With MEP, resistant levels of the Iri, Milyang, and Pyongtaek strains of N. cincticeps to the Suweon strain showed 13, 8, 7 and4 times, respectively. f. With MPP, resistant levels of the Iri and Pyongtaek strains of N. cincticeps were 7 and 4 times as compared with the Suweon strain, respectively. g. With NAC, resistant levels of the several strains of if. cincticeps showed no difference, and seemed not to have developed resistance yet.

• Journal of The Korean Society of Clinical Toxicology
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• v.5 no.1
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• pp.27-35
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• 2007

Purpose: Organophosphate (OP) compounds insecticides are the most commonly associated with serious human toxicity all over the world. The aim of this study was to identify sociocultural factors that contribute to high incidence of pure OP poisoning and prevent OP poisoning in order to reduce the factors responsible for deaths in South Korea. Methods: This is the 38 multi-centers survey and prospective study of pure OP poisoning by structural reporting system and hospital records from August 2005 to July 2006. 238 patients with acute pure OP poisoning were enrolled. We collected patient information regarding poisoning, clinical, and demographic features. Results: The mean age was $55.32{\pm}17.3$ years old. The most frequent site of exposure was their own residence(85.7%). Frequent compounds involving pure OP poisoning were dichlorvos(22.7%), methidathion(8.4%), and phosphamidon(6.7%). Intentional poisoning was 77.9%. The most frequently route of exposure was ingestion(94.5%). The mean arrival time to hospital after poisoning was 12.7 hours and mean hospitalization duration was 12.9 days. 2-PAM was administered to 101 patients in mean doses of 6.3 g/day intravenously. Atropine was administered to 81 patients in mean doses of 74.6 mg/day (maximal 910 mg/day). The presence of lower level of GCS score, respiratory complications, hypotension, acute renal failure, and serious dysrhythmia was associated with serious and fatal poisoning. Overall final mortality in pure OP poisoning was 9.7%(23/238). Conclusion: This study highlights the problem of pure OP poisoning in South Korea as a basic national survey. Futhermore this might help the establishment of strict policies availability of OP and the statistics of OP poison exposure in South Korea.

• Korean Journal of Veterinary Research
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• v.27 no.1
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• pp.153-155
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• 1987

Determination of cholinesterase activity is a routine practice in many laboratories to detect the influence of cholinesterase inhibiting drugs such as organophosphate and carbamate insecticides. Among many different methods to determine the cholinesterase activity, the present method was the most recent, simple and accurate one for routine test in clinics. The results obtained in sera of human and the different species of animals by means of the present method were as follows: $5.76{\pm}1.12U/ml$ in human, $3.37{\pm}0.83U/ml$ in german shepherd, $0.61{\pm}0.18U/ml$ in rat, $14.91{\pm}3.10U/ml$ in mouse, $1.55{\pm}0.51U/ml$ in chicken, $0.28{\pm}0.11U/ml$ in slaughtered cattle and $0.50{\pm}0.10U/ml$ in slaughtered pig.

• Microbiology and Biotechnology Letters
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• v.51 no.4
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• pp.374-389
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• 2023

Organophosphorus (OP) pesticides, particularly malathion, parathion, diazinon, and chlorpyrifos, are widely used in both agricultural and residential contexts. This refractory quality is shared by certain organ phosphorus insecticides, and it may have unintended consequences for certain non-target soil species. Bioremediation cleans organic and inorganic contaminants using microbes and plants. Organophosphate-hydrolyzing enzymes can transform pesticide residues into non-hazardous byproducts and are increasingly being considered viable solutions to the problem of decontamination. When coupled with system analysis, the multi-omics technique produces important data for functional validation and genetic manipulation, both of which may be used to boost the efficiency of bioremediation systems. RNA-guided nucleases and RNA-guided base editors include zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeats (CRISPR), which are used to alter genes and edit genomes. The review sheds light on key knowledge gaps and suggests approaches to pesticide cleanup using a variety of microbe-assisted methods. Researches, ecologists, and decision-makers can all benefit from having a better understanding of the usefulness and application of systems biology and gene editing in bioremediation evaluations.