• Journal of Microbiology and Biotechnology
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• v.34 no.4
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• pp.804-811
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• 2024

Foamy viruses (FVs) are generally recognized as non-pathogenic, often causing asymptomatic or mild symptoms in infections. Leveraging these unique characteristics, FV vectors hold significant promise for applications in gene therapy. This study introduces a novel platform technology using a pseudo-virus with single-round infectivity. In contrast to previous vector approaches, we developed a technique employing only two vectors, pcHFV lacking Env and pCMV-Env, to introduce the desired genes into target cells. Our investigation demonstrated the efficacy of the prototype foamy virus (PFV) dual-vector system in producing viruses and delivering transgenes into host cells. To optimize viral production, we incorporated the codon-optimized Env (optEnv) gene in pCMV-Env and the Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element (WPRE) at the 3' end of the transgene in the transfer vector. Consequently, the use of optEnv led to a significant enhancement in transgene expression in host cells. Additionally, the WPRE exhibited an enhancing effect. Furthermore, the introduced EGFP transgene was present in host cells for a month. In an effort to expand transgene capacity, we further streamlined the viral vector, anticipating the delivery of approximately 4.3 kbp of genes through our PFV dual-vector system. This study underscores the potential of PFVs as an alternative to lentiviruses or other retroviruses in the realm of gene therapy.

• Journal of Trauma and Injury
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• v.28 no.1
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• pp.51-54
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• 2015

The incidence of foreign body insertion in the anorectum is rare, however, it is increasing, especially in urban populations. Foreign objects in the anorectum can be of different sizes, shapes and materials. Frangible objects like glass or beakers, and sharp foreign bodies that may easily injure the bowel mucosa are particularly dangerous. Physicians have to consider more innovative options on how to extract these foreign bodies without inciting injury; and, if injury would occur, consider different techniques to repair it, whether transanally or transabdominally, and opt for primary repair, or resection with either anastomosis or stoma creation. Here, I introduce our cases with colorectal foreign bodies and present several literatures to help physicians decide when presented with cases like these.

• The Korean Journal of Pesticide Science
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• v.3 no.3
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• pp.11-19
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• 1999

A some of synthesized 2-(4-(6-chloro-2-benzoxazolyloxy)phenoxy)-N-phenyl propionamide derivativesa substrates were found to selectivity significantly with both rice plant (Oryza sativa L.) and barnyard grass (Echinochloa crus-galli) for those her- bicidal activities with post emergence in up land. The selectivity of substrates against rice plant better than that of Fenoxaprop-ethyl. The structure activity relationship (SAR) n the selectivity of N-phenyl substituents were analyzed by the Free-Wilson and Hansch method. The SAR approach against barnyard grass is shown that the optimal ($({\pi})_{opt.}=1.60$) hydrophobicity and electron donating effects ($0<{\sigma}$ & 0$(ES)_{opt.}=0.87$) so that the herbicidal activity against rice plant can be decreased. The significance of these results on the selectivity between barnyard grass and rice plant is discussed. And it is assumed that the 2-ethoxy-3-methoxy-4-dimethylamino group substituent ($pI_{50}$=6.60, 1g/ha) is selected as the most highest herbicidal activity against barngard grass in green house.

• International Commerce and Information Review
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• v.18 no.4
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• pp.195-217
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• 2016

The Convention on Contracts for the International Sale of Goods(CISG) endeavors to increase international trade through the creation of a uniform law of international sales. The CISG applies to contracts of sale of goods between parties whose places of business are in different States. If a party has more than one place of business, the place of business if that which has the closest relationship to the contract and its performance. Despite the importance of a definition for 'place of business,' the CISG does not provide one. Lack of a definition of 'place of business' may cause problems for parties trying to determine whether the CISG applies to their contract. Also Contracting parties can opt out of the CISG. But the CISG does not state whether the parties must expressly exclude the CISG's application to a transaction or whether they might do so by implication. we need to consider how effectively opt out of the CISG. Under U.S. law, the CISG is considered to be a self-executing treaty. So the CISG's provisions apply directly as substantive sales law to contracts for the international sale of goods. Despite the CISG's political and economic significance to U.S., U.S. Courts have overlooked the terms of the CISG. This article considered how to the CISG was recognized, interpreted and applied by the U.S. Courts in related cases.

• Journal of the Korean Wood Science and Technology
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• v.48 no.2
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• pp.196-205
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• 2020

The purpose of this study was to investigate a method for improving the physical and mechanical properties of laminated board made from oil palm trunk (OPT). The effects of pretreating the lamina with heat-pressure and altering the lamina composition of the laminated board were investigated. The outer third of OPT in cross-section had high-density wood, while the underlying third had low to medium density. The hot press was applied to pretreat the lamina that had low to medium density. The lamina were 1.5 cm in thickness, 5 cm in width, and 65 cm in length. The hot press was applied at 2.94 MPa or 4.41 MPa at 150 ℃ for 60 minutes, and the target thickness of the lamina was 1 cm. The three layers of the laminated board samples were bonded with isocyanate adhesive at a glue spread of 300 g/㎡ and cold pressed at 0.98 MPa for 3 h. The laminated board samples were tested according to Japanese Agricultural Standard (JAS) 234-2003. The results showed that the densification of the inner lamina did not significantly affect the physical-mechanical properties of the laminated board produced. However, the laminated board made with high-density laminas for the outer layers fulfilled the JAS 234-2003 standard for the modulus of elasticity and the modulus of rupture.

• Journal of the Korea Convergence Society
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• v.10 no.1
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• pp.265-275
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• 2019

The purpose of this study was to explore factors that physical education graduate students in graduate school of education drop out of teacher appointment examination of secondary school by using Ecological Model. The in-depth interview was conducted for 10 physical education graduate students studying in school of education. As an aspect of the intrapersonal component, the effort to apply how participants'current job positively affects their preparation for teacher appointment examination is required and they need to clearly decide academic priorities to prepare for teacher appointment examination. As an aspect of interpersonal component, participants are negatively affected by subjective norm, thereby providing personalized mentoring program is required. As an aspect of organizational component, limited tuition support and inappropriate place to study are emphasized, so practical helps like sports practice instructor or teacher appointment examination preparation class is required. Finally, this study suggests the introduction of integrated system for teacher appointment examination.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.44 no.4
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• pp.32-42
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• 2021

The printing process can have to print various colors with a limited capacity of printing facility such as ink containers that are needed cleaning to change color. In each container, cleaning time exists to assign corresponding inks, and it is considered as the setup cost required to reduce the increasing productivity. The existing manual method, which is based on the worker's experience or intuition, is difficult to respond to the diversification of color requirements, mathematical modeling and algorithms are suggested for efficient scheduling. In this study, we propose a new type of scheduling problem for the printing process. First, we suggest a mathematical model that optimizes the color assignment and scheduling. Although the suggested model guarantees global optimality, it needs a lot of computational time to solve. Thus, we decompose the original problem into sequencing orders and allocating ink problems. An approximate function is used to compute the job scheduling, and local search heuristic based on 2-opt algorithm is suggested for reducing computational time. In order to verify the effectiveness of our method, we compared the algorithms' performance. The results show that the suggested decomposition structure can find acceptable solutions within a reasonable time. Also, we present schematized results for field application.

• The Journal of Korean Academy of Orthopedic Manual Physical Therapy
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• v.29 no.1
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• pp.53-67
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• 2023

Background: This study comparatively evaluated the effects of equipment-based pilates exercises (EPE) and lumbar stability exercises (LSE) in patients with chronic low back pain in terms of their Visual Analogue Scale(VAS), Oswestry Disability Index(ODI), and abdominal muscle thickness. Methods: A total of 30 participants were recruited and randomly assigned to either the EPE or the LSE. The VAS, ODI, and abdominal muscle thicknesses of the participants were measured before and after the intervention. Results: The EPE were more effective in terms of the duration of a sustained reduction in VAS scores. post hoc test revealed that EPE were more efficacious in terms of a sustained improvement in ODI scores. With respect to changes in abdominal muscle thickness, there was a significant difference in the thickness of internal oblique muscles(IO) and the external oblique muscles(EO) between the two groups. Conclusion: In this study, both types of exercise interventions resulted in improvements in the VAS, ODI scores, and abdominal muscle thickness in patients with chronic low back pain. However, EPE were found to be more effective than LPE in terms of longer sustained improvements in VAS and ODI scores. Also, with respect to abdominal muscle thickness, the thickness of EO and IO improved only in the EPE group.

• The Journal of Korean Academy of Orthopedic Manual Physical Therapy
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• v.30 no.1
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• pp.75-83
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• 2024

Background: This study conducted a comparative evaluation of the effects of Pilates stabilization exercise combined with pain neuroscience education (PNE) in patients with chronic low back pain. The evaluation was based on their visual analogue scale (VAS) scores, Korean Oswestry disability index (KODI) scores, and fear avoidance belief questionnaire (FABQ) scores. Methods: A total of 36 participants were recruited and randomly assigned to either a Pilates stabilization exercise group (PSE, n=18) or Pilates stabilization exercise combined with pain neuroscience education group (PPNE, n=18). Both the PSE and PPNE groups participated in 50-minute sessions of Pilates stabilization exercise, three times per week for six weeks. The VAS, KODI, and FABQ scores of the participants were measured before and after the intervention. Results: There were significant improvements in the VAS of the PSE and PPNE group, with significant difference found between them. Both groups showed a significant decline in KODI scores following the exercise interventions, with significant difference observed between the two groups. FABQ scores were significantly decline in both groups, with significant difference found between them. Conclusion: In this study, Pilates stabilization exercise combined with pain neuroscience education was found to be more effective than Pilates stabilization exercise alone in reducing the VAS, KODI, and FABQ scores of patients with chronic low back pain. Thus, Pilates stabilization exercise combined with pain neuroscience education can be used in clinical practice to treat and prevent chronic lower back pain.

• The Korean Journal of Pesticide Science
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• v.4 no.3
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• pp.34-39
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• 2000

A new fifteen 2,3-dihydro-2-ethyl-2,4,6,7-tetramethylbenzofuran-5-yl substituents in 5-benzofuryl-2-[1-(alkoxyimino)alkyl]-3-hydroxycyclohex-2-en-1-one derivatives as substrate were synthesized and their herbicidal activities against rice plant (Oryza sativa L.) and barnyard grass (Echinochloa crus-galli) with post emergence were measured under submerged conditions. The $R_{1}$=methyl substituents, $1{\sim}8$ showed the higher herbicidal activity to the rice plant and barnyard grass. The structure-activity relationships (SARs) on tile herbicidal activity of $R_{1}$ and $OR_{2}$ groups on the azomethine bond in substrates were analysized. From tile results of dicussed SAR, the herbicide activities against rice plant would depend largely on the steric factor, Whereas, in case of barnyard grass, the activities were governed by the hydrophobicity factor. The conditions of selective herbicide activity between the two plant species are assumed that the substrates should nave optimal hydrophobicity ( $(logP)_{opt.}=6.0$), a $R_{1}$ groups of small and a long $OR_{2}$ groups.