• Korean Journal of Acupuncture
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• 제21권2호
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• pp.47-67
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• 2004

Objectives : In the present study, the effect of Scolopendrid Water-Alcohol Extract (SWAE) applied to acupuncture point BL23 (Shinsu) on the neuropathic pain was examined. A common source of persistent pain in humans is the neuropathic pain. Anti-convulsant drugs are used to treat the neuropathic pain. In the oriental medicine, Scolopendrid was used for long time to treat convulsant syndrome and back pain, etc. Methods : On the bases of the Scolopendrid clinical application, the effect of SWAE applied to the acupuncture point was tested in the rat model of neuropathic pain. Neuropathic pain was induced by tight ligation of L5 spinal nerve. When rats developed pain behaviors, One hundred microliter of SWAE was applied into the ipsilateral BL23 point at a dose of 10 mg/ml under enflurane anesthesia. The foot withdraw latency of the hind limb was measured for an indicator of pain level after each manipulation. Results : SWAE injection increased the mechanical threshold of the foot in the rat model of neuropathic pain significantly for the duration of 4h, suggesting a partial alleviation of pain. SWAE applied to BL23 point produced a significant improvement of mechanical sensitivity of the foot lasting for at least 4h. However, neither contralateral BL23 point, ST25 (Chonchu) point, nor LR3 (Taechung) point produce as much increase of mechanical sensitivity as ipsilateral BL23 point. And, this increase of mechanical sensitivity was dose-dependent. The improvement of mechanical threshold was interpreted as an analgesic effect. In addition, the analgesic effect of Scolopendrid 4 mg/kg injection is equivalent to that of gabapentin 50 mg/kg injection. The relations between SWAE-induced analgesia and endogenous nitric oxide(NO), inducible NO synthase (iNOS)/neuronal NO synthase (nNOS) were also examined. Results were turned out that both NO production and nNOS/iNOS protein expression which are increased by nerve injury were suppressed by SWAE injection applied to BL23 point. Conclusions : The data suggest 1) that SWAE produces a potent analgesic effect on the neuropathic pain model in the rat and 2) that SWAE-induced analgesia modulate endogenous NO through the suppression of nNOS/iNOS protein expression.

• 대한핵의학회지
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• 제29권4호
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• pp.518-525
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• 1995

목적 : 안구제거술 후에 삽입한 hydroxyapatite 안구보충물내로 혈관신생이 완전하게 일어 나는 시기를 알아보기 위함이다. 방법 : 안구제거술을 받은 43예의 환자(남자 33예, 여자 10예, 14-56세)에게 대부분 변경되지 않은 방법으로 hydroxyapatite 안구보충물을 삽입한 후 10-23주 사이에 다양한 간격(10-12 주 2예, 13-16주 18예, 17-20주 20예, 21-23주 3예)을 두고 $^{99m}Tc-MDP$ 골주사를 시행하여 보충물내로의 혈관신생 정도를 전향적으로 측정하였다. 정면 및 측면촬영의 정적영상에서 안구보충물의 방사능섭취가 비교(nasal bridge)의 방사능섭취보다 약할 경우를 grade 1, 같을 경을 grade 2, 강할 경우를 grade 3로 나누었으며 grade 2와 3을 혈관신생이 완전하다고 정의하였다. 또한 43예 모두에서 방사능섭취 정도와 관계없이 안구보충물 삽입후 평균 21주되는 시기에 안구보충물에 구멍을 내어 이 구멍으로부터의 출혈여부를 확인한 후 운동성나사(motility peg)를 끼워 의안(prosthesis)과 연결하였다. 43예중 7예에서는 $^{99m}Tc-MDP$ 골주사와 조영제주입후의 자기공명영상(Gd-DTPA T1-weighted images)을 같은 날짜에 시행하여 각각의 예에서 방사능섭취 정도와 조영제증강 정도를 비교하였다. 결과 : 안구보충물내로 완전하게 혈관신생이 일어났던 grade 2와 3의 비율은 안구보충물삽입후 10-12주에서는 0%, 13-16주 33%, 17-20주 50%, 21-23주 67%로써 시간경과에 따라 점차 증가하였다. 골주사소견에 관계없이 안구보충물 삽입후 평균 21주되는 시기에 보충물 앞쪽에 구멍을 내었을 때 43예 모두에서 완전한 혈관신생을 의미하는 출혈을 볼 수 있었다. $^{99m}Tc-MDP$ 골주사와 조영제주입후의 자기공명영상을 둘 다 시행한 7예 각각에서 방사능섭취의 정도와 조영제증강의 정도가 거의 일치하였다. 결론 : 본 예비연구의 결과로써 hydroryapatite 안구보충물 삽입후 21주째에 $^{99m}Tc-MDP$ 골주사를 시행하는 것이 좋을 것으로 생각되며, hydroxyapatite 안구보충물내로의 완전한 혈관신생 여부를 보는 데에는 $^{99m}Tc-MDP$ 골주사가 조영제주입후의 자기공명영상보다 더 경제적이라고 할 수 있겠다.

• Restorative Dentistry and Endodontics
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• 제36권5호
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• pp.367-376
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• 2011

치아우식증의 조기 발견은 외과적 삭제를 피하면서, 적절한 치료를 할 수 있는 좋은 기회를 제공한다. 광간섭단층촬영술은 최근 각광받기 시작한 3차원 이미지 기술로서, 안과에서 광학적 생검의 목적으로 빈번히 이용되는 것을 필두로 다양한 의학 분야에 적용되어 왔고, 최근 초기 우식증의 발견에 전도유망하여 다양한 연구가 진행 중이다. 이 기술은 저 상관도 간섭계의 원리에 근거하고 있으며, 장점으로는 비침습적이고, 방사선을 사용하지 않으며, 3차원 이미지 구축이 가능하다는 점이다. 본 연구에서는 광간섭단층촬영술의 원리와 개략적인 개발 과정에 대한 기술과 함께 치아우식증에 관한 연구들에 대하여 고찰해 보았고, 이를 통해 이 기술의 응용 가능성을 확인하였다. 그럼에도 불구하고, 임상적인 유용성을 입증하기 위해서는 몇 가지 기술적 문제를 해결해야 하고, 보다 많은 생체 내 실험이 뒷받침되어야 할 것이다.

• 대한약침학회지
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• 제21권3호
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• pp.151-158
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• 2018

Objectives: We investigated the efficacy and safety of miniscalpel acupuncture (MA) for knee osteoarthritis (KOA) in an assessor-blinded randomized controlled pilot trial; this would provide information for a large-scale randomized controlled trial. Methods: Participants (n = 24) were recruited and randomly allocated to the MA group (experimental) or acupuncture group (control). The MA group received treatment once a week for 3 weeks (total of 3 treatments), while the acupuncture group received treatment two times per week for 3 weeks (total of 6 treatments). The primary outcome was pain as assessed by a visual analogue scale (VAS). The secondary outcomes (intensity of current pain, stiffness, and physical function) were assessed using the short-form McGill Pain Questionnaire (SF-MPQ) and Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC). Assessments were performed at baseline, 1, 2, and 3 during treatment and at week 5 (2 weeks after the end of treatment). Results: Of the 24 participants, 23 completed the study. Both groups showed significant improvements in VAS, SF-MPQ, and WOMAC. However, there were no significant differences between the MA and acupuncture groups. No serious adverse event occurred and blood test results were within normal limits. Conclusion: Our results suggest that although both MA and acupuncture provide similar effects with regard to pain control in patients with KOA, MA may be more effective in providing pain relief because the same relief was obtained with fewer treatments. A large-scale clinical study is warranted to further clarify these findings.

• 한방재활의학과학회지
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• 제26권1호
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• pp.79-86
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• 2016

Objectives The purpose of this study was to compare the effects of MSAT (Motion style acupuncture treatment) using Sandbag regarding low back pain with HIVD of L-SPINE. Methods Forty patients received inpatient treatment from March 16 to May 16, 2015 in the Daejon-Jaseng oriental medicine hospital were divided into 2 groups by blocked randomization: Group A (n=20) is routine treatment on HIVD of L-SPINE with MSAT using Sandbag and Group B (n=20) is routine treatment on HIVD of L-SPINE (acupuncture, Shinbaro pharmacopuncture, herb medication, chuna treatment and physiotherapy). The MSAT (Motion style acupuncture treatment) using Sandbag was administered each day and both groups were received routine treatment each day. Evaluations were made before treatment, after 3 days and 7 days, and before discharge using the NRS (numeric rating scale) and ODI (oswestry disability index). The statistically significance was evaluated by SPSS 22.0 for windows. Results The NRS (numeric rating scale) and ODI (oswestry disability index) of both A and B groups after 3, 7 days and before discharge of treatment significantly decreased (p<0.05). Regarding group comparison, the NRS (numeric rating scale) and ODI (oswestry disability index) of A group was significantly lower than the B group at 3, 7 days, before discharge (p<0.05). Regarding improvement of the NRS (numeric rating scale) and ODI (oswestry disability index), A group was significantly lower than the B group at 3 day (NRS), 7 day (ODI) (p<0.05). There was significant difference between two groups at 7 days (NRS) and before discharge (p<0.05). Conclusions Compared to routine treatment, the MSAT (Mostion style acuputure treatment) using Sandbag significantly improved the NRS (numeric rating scale) and ODI (oswestry disability index) after 3, 7 days, and before discharge. Because early intervention determines the grade of Low back pain with HIVD of L-SPINE, the MSAT (Motion style acupuncture treatment) using Sandbag will be clinically helpful to patients at the early stage.

• Applied Microscopy
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• 제37권3호
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• pp.185-198
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• 2007

제주땃쥐 (Crocidura dsinezumi)의 정자변태과정은 전자현미경에 의해 조사되어졌으며, 이들 정자변태 과정은 핵의 형태적 특징과 세포질내 소기관의 변화에 기초하여 12기, 14단으로 구분되었다. 골지기$(1{\sim}2)$단의 정자세포의 핵은 모두 구형인데 반해 두모기$(3{\sim}6)$단의 정자세포의 핵은 타원형으로 변하였다. 정자꼬리는 첨체중기에서 생성되었으며, 성숙기에는 가늘고 긴 정자두부를 형성하였다. 정자세포의 두부는 1단에서 6단(골지기${\sim}$두모기)까지는 내강을 향하고 있었으며, 7단부터 14단(첨체기${\sim}$이탈기) 까지는 세정관 상피의 기저막 쪽으로 향하고 있었다. 핵과 첨체는 10단에서 각각 최대로 신장되었다. 염색질은 첨체후기 (10 단)에 응축되기 시작하여 성숙중기(12단)에 완전히 응축되고 균질화 되어졌다. 다포체의 출현은 두모중기(5단)에 첨체포 가까이에서 다포체가 출현하며 두모 후기(6단)에 이르러 골지체 가까이 다수가 관찰되었다. 이상의 결과를 종합하여 볼 때, 정자변태과정은 정자형성세포의 분화 단계를 분석하는데 유용한 정보를 제공해 주리라 여겨진다.

• 생명과학회지
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• 제18권1호
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• pp.23-29
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• 2008

인삼은 고전적으로 항당뇨 효과가 있는 것으로 보고되고 있다. Insulin-like growth factor (IGF) 역시 당뇨병성 신증의 발병 초기에 중요한 역할을 하는 것으로 알려져 있다. 이에 본 연구에서는 mesangial 세포에서 고포도당에 의한 IGF 분비에 대한 ginsenoside의 차단 효과 및 이와 관련된 신호전달계를 알아보았다. 결과는 다음과 같다. 고포도당에 의해 증가 되었던 IGF-I 및 IGF-II 분비 촉진 작용은 GTS, PD 및 PT 처리 시 차단되었으며, 세포 성장 촉진작용에서도 같은 효과를 볼 수 있었다. 아울러 고포도당에 의한 산화성 스트레스 종가, GSH 감소, AA 방출 증가 작용 및 $PGE_2$ 합성 증가 작용은 GTS 처리시 현저하게 차단되었으며 PD 및 PT 처리 시 역시 억제 되는 것으로 나타났다. 이상의 결과를 볼 때 mesangial 세포에서 ginsenoside는 산화성 스트레스 및 arachidonic acid 활성 경로를 억제하여 고포도당에 의한 IGFs 분비 작용을 차단하는 것으로 나타났다.

• Journal of Acupuncture Research
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• 제30권4호
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• pp.15-24
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• 2013

Objectives : This study was accomplished to investigate the effects of venesection with cupping therapy at Jeonjung($CV_{17}$) on the heart rate variability(HRV) in healthy adults. And based on that, we tried to find out how venesection with cupping therapy at Jeonjung($CV_{17}$) affects the activity and the balance of the autonomic nervous system. Methods : We investigated on 60 healthy volunteers consisted of 30 subjects in venesection with cupping therapy group and 30 subjects in control group. The study was established by randomized trial. venesection with cupping therapy was applied at Jeonjung($CV_{17}$) for 10 minutes in venesection with cupping therapy group. No treatment was executed in control group. We measured HRV 3 times : First, baseline measurement and two more times after treatment in venesection with cupping therapy group(after rest in control group). The SPSS 12.0 for Windows was used to analyze the data by paired t-test(in both group), independent-test(between the groups) and Chi-square test(between the sexs). Results : 1. In venesection with cupping therapy group, SDNN, Ln(TP), Ln(LF), Normalized LF, and LF/HF ratio increased significantly. Normalized HF decreased significantly. Mean HRT, RMSSD, PSI, Ln(HF), and Ln(VLF) were not affected. 2. In control group, Ln(HF) decreased significantly. Mean HRT, SDNN, RMSSD, PSI, Ln(TP), Ln(LF), Ln(VLF), Normalized LF, Normalized HF, and LF/HF ratio were not affected. Conclusions : This study suggests that venesection with cupping therapy at Jeonjung($CV_{17}$) increases the activity of the autonomic nervous system and has effects on the balance of the autonomic nervous system.

• 폴리머
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• 제38권3호
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• pp.364-370
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• 2014

망막색소상피(RPE)는 건강한 망막을 유지하는데 중요한 역할을 하고 RPE의 퇴화는 많은 망막질병을 유발한다. RPE 이식은 최근 망막 퇴화에 대한 가능성 있는 치료법으로 제시되고 있다. RPE 세포를 안전하게 이식하기 위해서는 지지체가 필요하므로 독특한 기계적 성질과 생체적합성을 갖는 실크를 사용하여 필름을 제조하였다. 실크필름의 FTIR, 접촉각 및 생분해성을 측정한 후, RPE 세포를 실크필름에 파종하여 그 영향을 확인하였다. MTT 분석, SEM, 면역형광염색, RT-PCR을 통해 세포의 부착, 생존도, 형태유지, 특이적 mRNA의 발현을 분석하였다. 본 연구에서는, 실크필름에 배양한 RPE 세포의 부착, 증식 및 표현형 유지가 뛰어남을 확인함으로써 실크필름의 망막 재생을 위한 조직 공학적 지지체로의 응용 가능성을 제시했다.

• 척추신경추나의학회지
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• 제5권2호
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• pp.169-180
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• 2010

Objectives: This study was carried out to investigate the relationship of leg length analysis and X-ray finding according to positions and valuation bases on diagnosis of pelvic malpositions. Methods: Twenty-two people who get $33.09{\pm}10.73$ as average were evaluated by leg length analysis and X-ray findings. After measuring innominate measurement(IM), femur head line. distance between S2 and posterior superior iliac spine(PSIS). ilium shadow measurement(ISM), major axis of obturator foramen(MaF), minor axis of obturator foramen(MiF) and distance between off centering measurement and symphysis pubis, those were analyzed. Results: 1. In assessment of posterior rotation malposition ilium(PI), it was showed the best coincidence between leg length analysis and X-ray analyzed by 1M in supine position(11 cases, 50.5%). 2. In assessment of inflare, coincidence index between leg length analysis and X-ray were not good($4.00{\pm}3.03$ cases, $18.15{\pm}13.82%$). And leg length analysis were not sensitive. 3. On the whole, coincidence index between leg length analysis and X-ray were not good(best: 1 case, 45.5%; worst: 11 cases, 50.0%). Conclusions: Results form this investigation showed the relationship of leg length analysis and X-ray according 10 positions and valuation bases on diagnosis of pelvic malpositions. This results are expected to contribute to establish method of assessment in diagnosis of pelvic malpositions.