Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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Spermiogenesis in the Crocidura dsinezumi

제주땃쥐 (Crocidura dsinezumi)의 정자변태

  • Jeong, Seung-Don (Division of Natural Science, College of Natural Sciences, Kyungnam University) ;
  • Lee, Jung-Hun (Division of Natural Science, College of Natural Sciences, Kyungnam University) ;
  • Oh, Hong-Shik (Department of Science Education, Cheju National University) ;
  • Kim, Sang-Sik (Electron Microscopy Facility, Department of Ophthalmology, Pusan National University Hospital)
  • 정승돈 (경남대학교 자연과학대학 자연과학부) ;
  • 이정훈 (경남대학교 자연과학대학 자연과학부) ;
  • 오홍식 (제주대학교 과학교육과) ;
  • 김상식 (부산대학교 대학병원 전자현미경실)
  • Published : 2007.09.30
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Abstract

Spermiogenesis in Japanese white-toothed shrew. Crocidura dsinezumi was investigated by transmission electron microscope. Spermiogenesis was divided into 12 phases 14 steps, based on the morphological features of the nucleus and change of organelles in cytoplasm. The nucleus of spermatids in Golgi (step $1{\sim}2$) phase were spherical; however, they were changed into oval in the cap (step $3{\sim}6$) phase. Flagellum appeared in the middle of acrosomal phase; on the other hand, slender and long spermatid head was formed in maturation phase. The head of spermatids faced the lumen in step 1 to step 6 (from Golgi to cap phase), but, in step 7 to step 14 (from acrosomal to spermiation phase), it turned its head to the basal lamina of the seminiferous epithelium. The nucleus and acrosome were elongated maximally in step 10. The condensation of chromatin started in late acrosomal (step 10) phase, and it was completely finished and homogenized in the middle of maturation (step 12) phase. Multivesicular body appeared near the acrosomal vacuole during the middle cap (step 5) phase, and a large number of them were observed near the Golgi apparatus in the late cap (step 6) phase. Considering all the results, the spermiogenesis might be useful information to analyse the differentiation of spermatogenic fells.

제주땃쥐 (Crocidura dsinezumi)의 정자변태과정은 전자현미경에 의해 조사되어졌으며, 이들 정자변태 과정은 핵의 형태적 특징과 세포질내 소기관의 변화에 기초하여 12기, 14단으로 구분되었다. 골지기$(1{\sim}2)$단의 정자세포의 핵은 모두 구형인데 반해 두모기$(3{\sim}6)$단의 정자세포의 핵은 타원형으로 변하였다. 정자꼬리는 첨체중기에서 생성되었으며, 성숙기에는 가늘고 긴 정자두부를 형성하였다. 정자세포의 두부는 1단에서 6단(골지기${\sim}$두모기)까지는 내강을 향하고 있었으며, 7단부터 14단(첨체기${\sim}$이탈기) 까지는 세정관 상피의 기저막 쪽으로 향하고 있었다. 핵과 첨체는 10단에서 각각 최대로 신장되었다. 염색질은 첨체후기 (10 단)에 응축되기 시작하여 성숙중기(12단)에 완전히 응축되고 균질화 되어졌다. 다포체의 출현은 두모중기(5단)에 첨체포 가까이에서 다포체가 출현하며 두모 후기(6단)에 이르러 골지체 가까이 다수가 관찰되었다. 이상의 결과를 종합하여 볼 때, 정자변태과정은 정자형성세포의 분화 단계를 분석하는데 유용한 정보를 제공해 주리라 여겨진다.

Keywords

References

  1. Adachi Y, Kurohmaru M, Hattori S, Hayashi Y: Spermatogenesis in the watase's shrew, Crocidura watasei a light electron microscopic study. Jikken Dobutsu 41 : 295-303, 1992
  2. Amann RP, Almquist JO: Reproductive capacity of dairy bulls. VII. Morphology of epididymal sperm. J Dairy Sci 45 : 1516-1526, 1962 https://doi.org/10.3168/jds.S0022-0302(62)89664-7
  3. Burgos MH, Fawcett DW: Studies on the fine structure of the mammalian testis. I. Differentiation of the spermatids in the cat (Felis domestica). J Biophys Biochem Cytol 1 : 287-315, 1955 https://doi.org/10.1083/jcb.1.4.287
  4. Clermont Y, Rambourg A, Hermo L: Connections between the various elements of the cis-and mid-compartments of the Golgi apparatus of early rat spermatids. Anat Rec 240 : 469-480, 1994 https://doi.org/10.1002/ar.1092400405
  5. Clermont Y, Tang XM: Glycoprotein synthesis in the Golgi apparatus of spermatids during spermiogenesis of the rat. Anat Rec 213 : 33-43, 1985 https://doi.org/10.1002/ar.1092130106
  6. Ekstedt E, Soderquist L, Ploen L: Fine structure of spermatogenesis and Sertoli cells (Epitheliocytus sustentans) in the bull. Anat Histol Embryol 15 : 23-48, 1986 https://doi.org/10.1111/j.1439-0264.1986.tb00529.x
  7. Fawcett DW, Ito S: The fine structure of bat spermatozoa. Am J Anat 116 : 567-610, 1965 https://doi.org/10.1002/aja.1001160306
  8. Garcia-Gil N, Pinart E, Sancho S, Badia E, Bassols J, Kádár E, Briz M, Bonet S: The cycle of the seminiferous epithelium in landrace boars. Animal Reprod Sci 73 : 211-225, 2002 https://doi.org/10.1016/S0378-4320(02)00144-6
  9. Goes RM, Dolder H: Cytological steps during spermiogenesis in the house sparrow (Passer domesticus, Linnaeus). Tissue Cell 34: 273-282, 2002 https://doi.org/10.1016/S0040-8166(02)00017-4
  10. Hattori S, Noboru Y, Yamanouchi K: Domestication of the watase's shrew, Crocidura horsfieldi watasei, for a laboratory animal. Japan J Exp Med 56 : 75-79, 1986
  11. Heo JC, Lee JH: Spermiogenesis in the Saghalien pygmy shrew, Sorex minutus gracillimus. Korean J Electron Microscopy 31 : 129-141, 2001.(Korean)
  12. Jung TD, Lee JH, Kim SS: Spermiogenesis in the Korean squirrel, Tamias sibiricus. Korean J Electron Microscopy 34 : 159-170, 2004.(Korean)
  13. Kim JW, Harding HR, Shorey CD: Electron-microscopic studies on the spermiogenesis and spermatozoa of the allied rock wallaby (Petrogale assimilus). Korean J Electron Microscopy 17 : 1-15, 1987.(Korean)
  14. Kojima Y, Ishikawa T: Etude au microscope électronique de la gouttelette protoplsmique observee dans le spermatozoide chez les les taureaux. Jpn J Vet Res 11: 152-158, 1963
  15. Krsti'c RV: Ultrastructure of the mammalian cell. Springer-Verlag Berlin Heidelberg, pp 1-125, 1979
  16. Kurohmaru M, Kobayashi H, Hattori S, Nishida T, Hayashi Y: Spermatogenesis and ultrastructure of a peculiar acrosomal formation in the musk shrew, Suncus murinus. J Anat 185 : 503-509, 1994
  17. Kurohmaru M, Tiba T, Nishida N, Hayashi Y: Spermatogenesis and ultrastructural changes of spermatids during spermiogenesis in the cotton rat, Sigmodon hispidus. Okajimas Folia Anat Jpn 65 : 203-220, 1988 https://doi.org/10.2535/ofaj1936.65.4_203
  18. Kwon AS, Lee YH: Comparative spermatology of anurans with special reference to phylogeny. In: Jamieson BGM, Ausio J, justine JL ed. Advances in spermatozoal phylogeny and taxanomy, pp. 321-332. Mem Mus Nat Hist Nat 1995
  19. Leblond CP, Clermont Y: Spermiogenesis of rat, mouse, hamster and guinea pig as revealed by the periodic acidfuchsin sulfurous acid technique. Am J Anat 90 : 167-215, 1952 https://doi.org/10.1002/aja.1000900202
  20. Lee JH: Spermiogenesis in the Korean manchurian field mouse, Apodemus spesiosus peninsulae. Korean J Electron Microscopy 26 : 221-233, 1996. (Korean)
  21. Lee JH, Choi BJ, Son SW: Spermiogenesis in the Korean greater horseshoe bat, Rhinolophus ferrumequinum korai. Korean J Electron Microscopy 22 : 97-117, 1992. (Korean)
  22. Lee JH, Son SW, Mori, Shiraishi S: Studies on the reproductive pattern in the male of Korean greater horseshoe bat, Rhinolophus ferrumequinum korai. II. Histological changes of cauda epididymis by sperm entrance, storage and disappearance. Korean J Zool 36 : 51-66, 1993. (Korean)
  23. Lee JH, Park KR: Sperm ultrastructure of Rana nigromaculata and Rana catesbeiana. Korean J Electron Microscopy 35 : 219-228, 2005. (Korean)
  24. Lin M, Harman A, Rodger JC: Spermiogenesis and spermiation in a marsupial, the tammar wallaby (Macropus eugenii). J Anat 190 : 377-395, 1997 https://doi.org/10.1046/j.1469-7580.1997.19030377.x
  25. Lin M, Jones RC: Spermiogenesis and spermiation in a monotreme mammal, the platypus, Ornithorhynchus anatinus. J Anat 196 : 217-232, 2000 https://doi.org/10.1046/j.1469-7580.2000.19620217.x
  26. Moreno RD, Ramalho-Santos J, Chan EKL, Wessel GM, Schatten G: The Golgi apparatus segregates from the lysosomal/ acrosomal vesicle during Rhesus spermiogenesis: Structural alterations. Dev Biol 219 : 334-349, 2000 https://doi.org/10.1006/dbio.2000.9606
  27. Morigaki T, Kurohmaru M, Kanai Y, Mukohyama M, Hondo E, Yamada J, Agungpriyono S, Hayashi Y: Cycle of the seminiferous epithelium in the Java fruit bat (Pteropus vampyrus) and the Japanese lesser horseshoe bat (Rhinolophus cornutus). J Vet Med Sci 63 : 773-779, 2001 https://doi.org/10.1292/jvms.63.773
  28. Olson GE, Winfrey VP: Mitochondrial-cytoskeleton interactions in the sperm midpiece. J Ultrastruct Biol 103 : 13-22, 1990
  29. Patil SB, Saidapur SK: Kinetics of spermatogenesis in the wild squirrel Funambulus palmarum (Linnaeus). Acta Anat 141 : 352-363, 1991 https://doi.org/10.1159/000147147
  30. Phillips DM: Spermiogenesis. Academic Press., New York, pp 16-17, 1974
  31. Ploen L, Ekwall H, Afzelius BA: Spermiogenesis and the spermatozoa of the European common shrew (Sorex araneus L.). J Ultrastr Res 68 : 149-159, 1979 https://doi.org/10.1016/S0022-5320(79)90150-3
  32. Son SW, Lee JH: Spermiogenesis in the Korean striped field mouse (Apodemus agrarius coreae). Korean J Zool 38 : 395-404, 1995.(Korean)
  33. Son SW, Lee JH: Spermiogenesis in the Korean red-backed vole, Clethrionomys rufocanus regulus. Korean J Biomed Lab Sci 2 : 57-69, 1996.(Korean)
  34. Son SW, Lee JH, Cheon HM: Spermiogenesis in the Korean daubenton's bat (Myotis daubentonii ussuriensis). Dev Reprod 1 : 9-24, 1997.(Korean)
  35. Son SW, Lee JH, Choi BJ, Choi YM: Spermiogenesis in the Korean cat (Felis domestica). Korean J Zool 37 : 416-427, 1994.(Korean)
  36. Susi FR, Leblond CP, Clermont Y: Changes in the Golgi apparatus during spermiogenesis in the rat. Am J Anat 130 : 251-268, 1971 https://doi.org/10.1002/aja.1001300302
  37. Tait AJ, Johnson E: Spermatogenesis in the grey squirrel (Sciurus carolinensis) and changes during sexual regression. J Repod Fert 65 : 53-58, 1982 https://doi.org/10.1530/jrf.0.0650053
  38. Tang XM, Lalli MF, Clermont Y: A cytochemical study of the Golgi apparatus of the spermatid during spermiogenesis in the rat. Am J Anat 163 : 283-294, 1982 https://doi.org/10.1002/aja.1001630402
  39. Toshimori K, Tanii I, Araki S, Oura C: Characterization of the antigen recognized by a monoclonal antibody MN9: Unique transport pathway to the equatorial segment of sperm head during spermiogenesis. Cell Tissue Res 270 : 459-468, 1992 https://doi.org/10.1007/BF00645047
  40. Vogl AW, Lin YC, Dym M, Fawcett DW: Sertoli cells of the golden-mantled ground squirrel (Spermophilus lateralis) : A model system for the study of shape change. Am J Anat 168 : 83-98, 1983 https://doi.org/10.1002/aja.1001680109
  41. Yasumoto A, Masmichi K, Shosaku H, Yoshihiro H: Spermatogenesis in the watase's shrew, Crocidura watasei.-A light and electron microscopic study-. Exp Anim 41 : 295-303, 1992 https://doi.org/10.1538/expanim1978.41.3_295