Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Ginsenosides Protect the High Glucose-induced Stimulation of IGFs in Mesangial Cells

Mesangial 세포에서 고포도당에 의해 유도되는 insulin-like growth factor 분비 촉진작용에 대한 ginsenosides의 차단 효과

  • Bae, Chun-Sik (College of Veterinary Medicine, Biotechnology Research Institute,Chonnam National University) ;
  • Lim, Do-Seon (Department of Dental Hygiene, Eulji University) ;
  • Yoon, Byeong-Cheol (College of Veterinary Medicine, Biotechnology Research Institute,Chonnam National University ) ;
  • Jeong, Moon-Jin (Department of Oral Histology, College of Dentistry, Chosun University) ;
  • Yoon, Kyung-Chul (Department of Ophthalmology, Chonnam National University, Medical School and Hospital) ;
  • Park, Soo-Hyun (Functional Food Center, Chonnam National University)
  • 배춘식 (전남대학교 수의과대학 및 생물공학연구소) ;
  • 임도선 (을지대학교 보건과학대학 치위생학과) ;
  • 윤병철 (전남대학교 수의과대학 및 생물공학연구소) ;
  • 정문진 (조선대학교 치과대학 구강조직학교실) ;
  • 윤경철 (전남대학교 의과대학 안과학교실) ;
  • 박수현 (전남대학교 기능성 식품센터)
  • Published : 2008.01.31
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Abstract

Panax ginseng C. A. MEYER is one of the most widely used herbal medicines in the Asian countries and has diverse functions including anti-diabetic action. The dysfunctions of mesangial cells in hyperglycemic conditions are implicated in the development of diabetic nephropathy. Insulin-like growth factors (IGFs) are also associated with the onset of diabetic nephropathy. Thus, we examined the effect of ginsenosides against high glucose-induced dysfunction of primary cultured rat mesangial cells. In the present study, high glucose increased IGF-I and IGF-II secretion in mesangial cells. Ginsenoside total saponin (GTS) prevented high glucose-induced increase of IGF-I and IGF-II secretion in mesangial cells. In addition, GTS prevented high glucose-induced increase of lipid peroxide formation and decrease of GSH contents. GTS also ameliorates high glucose-induced increase of arachidonic acid release and decrease of prostaglandin $E_2$. In conclusion, GTS prevented high glucose-induced dysfunction of mesangial cells via inhibition of oxidative stress and arachidonic acid pathways.

인삼은 고전적으로 항당뇨 효과가 있는 것으로 보고되고 있다. Insulin-like growth factor (IGF) 역시 당뇨병성 신증의 발병 초기에 중요한 역할을 하는 것으로 알려져 있다. 이에 본 연구에서는 mesangial 세포에서 고포도당에 의한 IGF 분비에 대한 ginsenoside의 차단 효과 및 이와 관련된 신호전달계를 알아보았다. 결과는 다음과 같다. 고포도당에 의해 증가 되었던 IGF-I 및 IGF-II 분비 촉진 작용은 GTS, PD 및 PT 처리 시 차단되었으며, 세포 성장 촉진작용에서도 같은 효과를 볼 수 있었다. 아울러 고포도당에 의한 산화성 스트레스 종가, GSH 감소, AA 방출 증가 작용 및 $PGE_2$ 합성 증가 작용은 GTS 처리시 현저하게 차단되었으며 PD 및 PT 처리 시 역시 억제 되는 것으로 나타났다. 이상의 결과를 볼 때 mesangial 세포에서 ginsenoside는 산화성 스트레스 및 arachidonic acid 활성 경로를 억제하여 고포도당에 의한 IGFs 분비 작용을 차단하는 것으로 나타났다.

Keywords

References

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