• Asian-Australasian Journal of Animal Sciences
• /
• v.30 no.5
• /
• pp.736-742
• /
• 2017

Objective: Experiments were conducted to clone the sequence of Wild Argali short palate, lung and nasal epithelium clone 1 (SPLUNC1) cDNA, and to lay the foundation for further study the biological function of Wild Argali SPLUNC1. Methods: The complete sequence of Wild Argali SPLUNC1 cDNA was generated by rapid amplification of cDNA ends. The entire coding sequence was inserted into the pPIC9K vector and expressed in Pichia pastoris (P. pastoris) GS115. The recombinant SPLUNC1 protein was detected by Western blot and purified by $Ni^{2+}$ chelate affinity chromatography. The test of effect of the protein on Mycoplasma ovipneumoniae (MO) was performed with real-time polymerase chain reaction. Results: The Wild Argali SPLUNC1 cDNA was 1,076 bp with an open reading frame of 768 bp, which encoded a 26.49 kDa protein composed of 255 amino acids. Its amino acid sequence shared 98.4%, 96.9%, 94.5%, 90.2%, 80.8%, 78.4%, 78.3%, 72.5%, 72.3%, 68.8% identity with those of SPLUNC1 cDNA from Ovis aries (accession no. NP_001288334.1), Capra hircus (accession no. XP_005688516.1), Pantholops hodgsonii (accession no. XP_005979709.1), Bos taurus (accession no. NP_776851.1), Felis catus (accession no. XP_006929910.1), Homo sapiens (accession no. NP_001230122.1), Sus scrofa (accession no. NP_001005727.1), Chinchilla lanigera (accession no. NP_001269294.1), Mus musculus (accession no. NP_035256.2), and Rattus norvegicus (accession no. NP_742028.1), respectively. The recombinant protein corresponded to the expected molecular mass of 25.47 kDa as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and it was detected in the supernatant of P. pastoris, and it could be purified. The results from the test of inhibition effect of argali recombinant SPLUNC1 protein on MO showed that the product could inhibit MO very well (p<0.01). Conclusion: The amino acid sequence of Wild Argali SPLUNC1 was different from other organisms. The recombinant SPLUNC1 protein has good biological activity.

• The Journal of Korean Physical Therapy
• /
• v.32 no.4
• /
• pp.238-244
• /
• 2020

Purpose: This study compared the effects of the fascial distortion model (FDM), foam rolling (FR), and self-stretching (SS) on the ankle dorsiflexion range of motion (ROM). Methods: Thirty subjects who had no more than 30° of ankle dorsiflexion ROM at the weight-bearing lunge test were recruited in this study. They were divided into three groups: (FDM, FR, and SS), and underwent each intervention for five minutes. Before and after the intervention, the ankle dorsiflexion ROM in the supine (the open-) and standing (the closed-kinetic chain) of the subjects were tested. The changes in the ROM between pre- and post-intervention and among the groups were analyzed. Results: All groups showed increased ankle dorsiflexion ROM after the intervention in both positions. In the position of the open kinetic chain, the changes in the ROM between pre- and post-intervention had significant differences among the groups, and the FDM was higher than the FR and SS. In the position of the closed kinetic chain, the ROM after the interventions and the changes in the ROM had significant differences among the groups, and FDM was higher than the FR (ROM after the intervention, the change in ROM) and SS (the change in ROM). Conclusion: These findings showed that FDM had more efficiency than the FR and SS as FDM had a stronger effect on increasing ankle dorsiflexion in a short, limited time. Clinicians who have limited time to treat their patients, particularly trying to increase ankle dorsiflexion ROM, should consider the application of FDM.

• KIISE Transactions on Computing Practices
• /
• v.21 no.3
• /
• pp.173-182
• /
• 2015

Motion control systems are widely deployed in various industrial automation processes. The motion controller, which is a key element of motion control systems, has stringent real-time constraints. The controller must provide a short and deterministic control message transmission cycle, and minimize the actuation deviation among motor drives. To meet these requirements, hardware-based proprietary controllers have been prevalent. However, since it is becoming difficult for such an approach to meet increasing needs of system interoperability and scalability, nowadays, software-based universal motion controllers are regarded as their substitutes. Recently, embedded motion controller solutions are gaining attention due to low cost and relatively high performance. In this paper, we designed and implemented an embedded motion controller on an ARM-based evaluation board by using Xenomai real-time kernel and other open source software components. We also measured and analyzed the performance of our embedded controller under a realistic test-bed environment. The experimental results show that our embedded motion controller can provide relatively deterministic performance with synchronized control of three motor axis at 2 ms control cycle.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
• /
• v.24 no.5
• /
• pp.94-105
• /
• 2010

To detect faults in an inverter-fed permanent magnet synchronous motor (PMSM) drive under the circumstance having faults in a stator winding and inverter switch, an on-line basis fault detecting scheme during operation is presented. The proposed scheme is achieved by monitoring the second-order harmonic component in q-axis current and the fault is detected by comparing these components with those in normal conditions. The linear interpolation method is employed to determine the harmonic data in normal operating conditions. As soon as the fault is detected, the operating mode is changed to identify a fault type using the phase current waveform. To verify the effectiveness of the proposed fault detecting scheme, a test motor to allow inter-turn short in the stator winding has been built. The entire control algorithm is implemented using DSP TMS320F28335. Without requiring an additional hardware, the fault can be effectively detected by the proposed scheme during operation so long as the steady-state condition is satisfied.

• Biotechnology and Bioprocess Engineering:BBE
• /
• v.11 no.6
• /
• pp.489-495
• /
• 2006

In recent years, there has been an interest to use Moringa oleifera as the natural coagulant due to cost, associated health and environmental concerns of synthetic organic polymers and inorganic chemicals. However, it is known that M. oleifera as the natural coagulant is highly biodegradable and has a very short shelf life. This research was carried out to investigate the effects of storage temperature, packaging methods, and freeze-drying on the preservation of M. oleifera seeds powders. Non freeze-dried M. oleifera was prepared into different packaging namely open container, closed container and vacuum packing, whilst, freeze-dried M. oleifera was stored in closed container and vacuum packing. Each of the packaging was stored at room temperature ($30\;to\;32^{\circ}C$) and refrigerator ($4^{\circ}C$). The turbidity removal efficiencies of stored M. oleifera were examined using jar test at monthly interval for 12 months. The results indicated that non freeze-dried M. oleifera kept in the refrigerator ($4^{\circ}C$) would preserve its coagulation efficiency. In addition, closed container and vacuum packing were found to be more appropriate for the preservation of non freeze-dried M. oleifera, compared to open container. Freeze-dried M. oleifera retained its high coagulation efficiency regardless the storage temperature and packaging method for up to 11 months. Besides, higher increment in zeta potential values for water coagulated with freeze-dried M. oleifera indicated the higher frequency of charge neutralization and better coagulation efficiency of freeze-dried M. oleifera, compared to non freeze-dried seeds. As a coagulant, M. oleifera did not affect the pH of the water after treatment.

• Transactions of the Korean hydrogen and new energy society
• /
• v.32 no.5
• /
• pp.315-323
• /
• 2021

The air-cooled proton exchange membrane fuel cells (PEMFC) stacks, which is widely used in small-sized PEMFC, have a problem in that durability is weaker than that of the water-cooled type. Because the cathode is open to the atmosphere and the structural problem of the air-cooled stack, which is difficult to maintain airtightness, is highly likely to form a hydrogen/air boundary during start-up/shut-down (SU/SD). Through the accelerated durability evaluation of the 20 W air-cooled PEMFC stack, the purpose of this study was to find out the cause of the degradation of the stack and to contribute to the improvement of the durability of the air-cooled PEMFC stack. In this study, it was possible to evaluate durability in a relatively short time by reducing 20-30% of initial performance by repeating SU/SD 1,000 to 1,200 times on an air-cooled PEMFC stack. After disassembling the stack, each cell was divided into two and the performance analysis showed that the electrode degradation was more severe in the anode outlet membrane electrode assembly (MEA), which facilitates air inflow as a whole, than in the inlet MEA. It was shown that the cathode Pt was dissolved/precipitated to deteriorate the polymer ionomer inside the membrane.

• Composites Research
• /
• v.13 no.6
• /
• pp.47-54
• /
• 2000

One promising method for impact detection of composite structures is based on the use of piezopolymer thin fim (PVDf) sensor. In this paper, the relationship between the contact force and the signals of the attached strain gage and PVDF sensor to the composite plate subjected to low-velocity impact were derived. The relation for the open circuit and short circuit voltage of PVDF sensor was derived based on the equivalent circuit model of the piezoelectric sensor. The work was then extended to include experimental investigation into the use of short circuit voltage of PVDF sensor without using charge amplifier to detect low-velocity impact. The natural frequencies and damping ratio of the composite plate obtained from the vibration test were used to modify the analytical model and therefore the differences between measured and simulated signal of the modified analytical model in both forward and backward problem were considerably reduced. The reconstructed contact force and simulated sensor signals agreed well with the measured contact force, strain gage signal, and PVDF sensor singanl.

• Journal of the Korean Vacuum Society
• /
• v.10 no.1
• /
• pp.27-30
• /
• 2001

Electromigration Phenomena in Al-1%Si thin film interconnections under DC and PDC conditions were investigated. Thin film interconnections with $SiO_2$ and PSG/$SiO_2$ dielectric passivation layer were formed by a standard photolithography process method and test line lengths were 100, 400, 800, 1200, and 1600 $\mu\textrm{m}$. The current density of $1.19\times10^7\textrm{A/cm}^2$ was stressed in Al-1%Si thin film interconnections under DC condition. The current density of $1.19\times10^7\textrm{A/cm}^2$ was also applied under PDC condition at the frequency of 1 Hz with the duty factor of 0.5. The electromigration resistance of PSG/SiO2 dielectric passivation test line was stronger than $SiO_2$ dielectric passivation test line. The lifetime under PDC was 2-4 times longer than DC condition. As the thin film interconnection line increased, the lifetime decreased and saturated over the critical length. Failure patterns by an electromigration were dominated by void-induced electrical open and hillock-induced electrical short.

• The Journal of the Institute of Internet, Broadcasting and Communication
• /
• v.14 no.5
• /
• pp.215-221
• /
• 2014

In this paper, the improved power durability test system and method for an reliability analysis of SAW device is proposed and the failure mechanism through failure analysis is analyzed. As a result of the failure analysis using microscope, SEM and EDX, the failure mechanism of the SAW device is electromigration due to joule heating under high current density and high temperature condition. The electromigration makes voids and hillocks in the IDT electrode and the voids and hillocks can lead to short circuit and open circuit faults, respectively, increasing the insertion loss of an SAW filter. The accelerated life testing of the SAW filter for 450MHz CDMA application using the proposed power durability test system and method is carried out. $B_{10}$ lifetime of the SAW filter using Eyring model and Weibull distribution is estimated as about 98,500 hours.

• Biomolecules & Therapeutics
• /
• v.27 no.2
• /
• pp.160-167
• /
• 2019

Depression is a major mood disorder. Abnormal expression of glial glutamate transporter-1 (GLT-1) is associated with depression. Schisantherin B (STB) is one bioactive of lignans isolated from Schisandra chinensis (Turcz.) Baill which has been commonly used as a traditional herbal medicine for thousands of years. This paper was designed to investigate the effects of STB on depressive mice induced by forced swimming test (FST). Additionally, we also assessed the impairment of FST on cognitive function in mice with different ages. FST and open field test (OFT) were used for assessing depressive symptoms, and Y-maze was used for evaluating cognition processes. Our study showed that STB acting as an antidepressant, which increased GLT-1 levels by promoting PI3K/AKT/mTOR pathway. Although the damage is reversible, short-term learning and memory impairment caused by FST test is more serious in the aged mice, and STB also exerts cognition improvement ability in the meanwhile. Our findings suggested that STB might be a promising therapeutic agent of depression by regulating the GLT-1 restoration as well as activating PI3K/AKT/mTOR pathway.