• Title, Summary, Keyword: Oncorhynchus mykiss

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Effects of High Intracellular Calcium Concentration by Ouabain on VTG Production in the Primary Hepatocyte Cultures of Rainbow Trout, Oncorhynchus mykiss. (무지개송어(Oncorhynchus mykiss)간세포배양에 있어서 Ouabain에 의한 세포내 고Calcium 농도가 Vitellogenin 합성에 미치는 효과)

  • Yeo, In-Kyu
    • Journal of Aquaculture
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    • v.11 no.2
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    • pp.279-282
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    • 1998
  • Effects of high concentration of intracellular calcium on estradiol-induced vitellogenin(VTG) induction were examined using ouabain in Primary hepatocyte culture in the rainbow trout Oncorhynchus mykiss. Ouabain increases cytosolic free calcium as a result of inhibition of $Na^+ - Ca^{2+}$ exchanger. Ouabain markedly reduced VTG production to the control level, despite of calcium concentrations in the incubatin medium. Therefore, ouabain would reduce VTG production not by increasing intracellular calcium bt directly by inhibiting $Na^+ - K^+$ ATPase.

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Antioxidant and Cholinesterase Inhibitory Activities of Aqueous Extract from Rainbow Trout Oncorhynchus mykiss

  • Baek, Jae-Min;Yoon, Na-Young;Kim, Yeon-Kye;Lee, Doo-Seog;Yoon, Ho-Dong;Park, Jeung-Sook
    • Fisheries and aquatic sciences
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    • v.14 no.2
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    • pp.89-92
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    • 2011
  • We investigated the antioxidant and cholinesterase inhibitory activities of the aqueous extract of rainbow trout Oncorhynchus mykiss. The antioxidant activity of O. mykiss aqueous extract was determined by in vitro peroxynitrite scavenging activity and reducing power assays. The aqueous extract of O. mykiss showed potent peroxynitrite radical scavenging activity ($IC_{50}=0.12{\pm}0.001\;mg/mL$) and reducing power (absorbance=$0.47{\pm}0.001$) at the concentration of 1 mg/mL. The in vitro cholinesterase inhibitory activity of O. mykiss aqueous extract was examined using spectrophotometric analyses of acetyl- and butyrylcholinesterase. The aqueous extract of O. mykiss showed acetylcholinesterase inhibitory activity ($IC_{50}=1.61{\pm}0.13\;mg/mL$), but did not exhibit inhibitory activity against butyrylcholinesterase. These results suggest that O. mykiss possesses antioxidant and acetylcholinesterase inhibitory activities and provide scientific evidence for the health benefits of O. mykiss aqueous extract.

Morphometric Comparison in Rainbow Trout, Oncorhynchus mykiss, Coho Salmon, Oncorhynchus kisutch, and their Allotriploid (무지개송어, 은연어 및 그 잡종3배체의 체형 비교)

  • HONG Kyung-pyo;KIM Pyong-Kih;MYOUNG Jung-Goo;KIM Jong-Man;HUH Hyung-Tack
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.30 no.4
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    • pp.687-689
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    • 1997
  • For identification of morphometric characteristics in rainbow trout, Oncorhynchus mykiss, coho salmon, O. kisutch, and their allotriploid, twenty one body traits were measured, and the ratios of each trait to fork length, length from postorbital to the origin of caudal fin and head length were calculated. The ratios of fin length to fin height in anal, dorsal and adipose fins showed significant differences among these two species and their allotriploid $(p\leq0.01)$. Allotriploid could be distinguished easily from two parents species in the ratios of length of origin of ventral fin to fork length and anal fin length to anal fin height.

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Comparison of wound healing effects between Oncorhynchus keta-derived polydeoxyribonucleotide (PDRN) and Oncorhynchus mykiss-derived PDRN

  • Lee, Jong Hun;Han, Jin Woo;Byun, Jin Hwan;Lee, Won Mi;Kim, Min Ho;Wu, Wen Hao
    • Archives of Craniofacial Surgery
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    • v.19 no.1
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    • pp.20-34
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    • 2018
  • Background: Polydeoxyribonucleotide (PDRN) influencing cellular growth and differentiation is recognized to promote wound healing by stimulating tissue repair. Although PDRN can be extracted from human placentas, PDRN medications have recently been extracted from the semen of trout (Oncorhynchus mykiss) and salmon (Oncorhynchus keta). The present study was designed to evaluate the wound healing effects of O. keta-derived PDRN for injection (Rejuvenex) and PDRN cream (Rejuvenex Cream) in comparison with those of O. mykiss-derived PDRN injection (Placentex). Methods: Full-thickness skin defects were made on the back of mice (n=60). The mice were divided into the following four groups according to the dressing used for the wounds: O. mykiss-derived PDRN injection group, O. keta-derived PDRN injection group, O. ketaderived PDRN cream group, and normal saline soaked dressing group (control group). We analyzed the gross findings, wound sizes, histological findings, immunohistochemistry and enzyme-linked immunosorbent assays for the groups immediately after the treatment, and again after 4, 7, and 10 days of treatment. Results: The wound healing effects were the greatest in the O. keta-derived PDRN injection and O. mykiss-derived PDRN injection groups, which showed similar scores, followed by the O. keta-derived cream and normal saline soaked dressing groups. Conclusion: The injection of PDRN extracted from O. keta was found to be as effective at healing full-thickness skin defects as the O. mykiss-derived PDRN injection, which is currently used in the clinic. Moreover, the O. keta-derived PDRN injection was also found to reduce the time required for wound healing.

Initial Transcriptome Profile of Rainbow Trout (Oncorhynchus mykiss) Liver

  • Kim Soonhag
    • Fisheries and aquatic sciences
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    • v.6 no.1
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    • pp.41-44
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    • 2003
  • Ninety nine random complementary DNA clones from rainbow trout (Oncorhynchus mykiss) liver cDNA library were partially sequenced as one approach to analyze the transcribed sequences of its genome. Of the sequence generated, $64.0\%$ of the ESTs were represented by 29 known genes. Thirty six clones of the unknown gene products potentially represent 31 unique genes. Serum albumin $(16.1\%)$ was the most abundant in the liver. The structural genes in the liver $(19\%)$ were the highly expressed functional category. This research is helpful to understand tissue specific gene expression profile and basic relationship between tissue and functional categories of the genes.

Immune gene expression following LPS exposure in the gill of rainbow trout, Oncorhynchus mykiss

  • Hong, Su-Hee
    • Journal of fish pathology
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    • v.18 no.3
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    • pp.287-292
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    • 2005
  • In the present study, immune gene expression of rainbow trout, Oncorhynchus mykiss, against bacterial endotoxin (LPS) was studied in vivo. The expression of prointflammatory cytokine genes (IL-1$\beta$ and TNF-$\alpha$) and IFN-related genes (IRF-I, Mx-3) at gill was assessed by RT-PCR at different time point of day1 and day 3 post-injection. It was shown that the proinflammatory cytokine gene expression at gill was induced 1 day after LPS injection but the expression was not sustained until day 3. Meanwhile upregulated expression of IFN-related genes was found to be only at day 3 post injection, indicating indirect effect of LPS on these genes.

Effects of ultrasonic and microbubble treatment on serum components and innate immunity in rainbow trout (Oncorhynchus mykiss) (초음파와 microbubble 처리가 무지개송어(Oncorhynchus mykiss)의 혈청성분 및 선천성 면역에 미치는 영향)

  • Kim, Jin-Young;Park, Jong-Bin;Park, Jeong Su;Kim, Woo-Seoung;Kim, Hak Soo;Kwon, Se Ryun
    • Journal of fish pathology
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    • v.32 no.2
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    • pp.91-96
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    • 2019
  • Rainbow trout (Oncorhynchus mykiss) were treated with ultrasonic (40kHz) and microbubble. The organic serum components, glucose were decreased compared to control at microbubble single treatment (M) and ultrasonic treatment (UL), but glucose concentrations in fish serum of all experimental groups analyzed in this study were also within the normal range. The organic serum components, cholesterol and total protein were no significant change by ultrasonic and microbubble treatment. There were no significant changes in the lysozyme activity on ultrasonic and microbubble treatment. Serum bactericidal activity decreased on ultrasonic single treatment (UL) compared to the control (P<0.05), but the numbers of bacterial colonies were significantly lower than those in the negative (PBS). The results of this study indicate that ultrasonic and microbubble treatment did not have a critical effect on the serum composition and innate immunity of O. mykiss, and that it is a way to do not significant stress on the fish body as a countermeasure against sea lice.

Comparison of the Food Quality of Freshwater Rainbow Trout Oncorhynchus mykiss Cultured in Different Regions (생산 지역을 달리한 담수산 무지개송어(Oncorhynchus mykiss)의 식품학적 품질 특성 비교)

  • Kang, Sang In;Kim, Ki Hyun;Lee, Jun Kyu;Kim, Yong Jung;Park, Su-Jin;Kim, Min Woo;Choi, Byeong Dae;Kim, Dongsoo;Kim, Jin-Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.47 no.2
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    • pp.103-113
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    • 2014
  • This study compared the food quality of freshwater rainbow trout Oncorhynchus mykiss cultured (in Pyeongchang, Pyeongtaek, Jecheon and Geochang) with that of imported salmon Oncorhynchus keta. The proximate composition of the four types of rainbow trout was 74.4-75.7% moisture, 18.3-19.4% crude protein, 3.1-4.3% crude lipid, and 1.3-1.7% ash. There were slight differences in the proximate composition of the four types of rainbow trout. No significant (P>0.05) differences were found in the red color and odor and taste intensities of the four types of rainbow trout based on the Hunter a values, volatile basic nitrogen content, odor intensity using an electronic nose and taste intensity using an electronic tongue. The crude protein, total amino acid, and mineral contents of rainbow trout cultured in Pyeongtaek were superior or similar to those of the trout cultured in the other three places. The rainbow trout cultured in Geochang had the highest hardness, at 858.5 g.

Monitoring of Viral Hemorrhagic Septicemia Virus in Seawater-Reared Rainbow Trout Oncorhynchus mykiss (해수 사육 무지개송어(Oncorhynchus mykiss)의 Viral Hemorrhagic Septicemia Virus (VHSV) 모니터링)

  • Kim, Wi-Sik;Kong, Kyoung-hui;Jeon, Young-Ho;Oh, Myung-Joo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.50 no.5
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    • pp.621-623
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    • 2017
  • Mariculture of rainbow trout Onchorhynchus mykiss has been initiated in or around olive flounder Paralichthys olivaceus farms, where viral hemorrhagic septicemia virus (VHSV) is often detected in some fish. In the present study, we investigated VHSV infection in seawater-reared rainbow trout because VHSV has never been detected in salmonids in Korea. A total of 104 adult fish were tested for the presence of VHSV by reverse transcription-polymerase chain reaction, followed by virus isolation with the fathead minnow caudal trunk cell line. Cytopathic effects were observed in two samples but the virus was identified as infectious hematopoietic necrosis virus. Thus, VHSV was not isolated from seawater-reared rainbow trout.