Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
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Effects of High Intracellular Calcium Concentration by Ouabain on VTG Production in the Primary Hepatocyte Cultures of Rainbow Trout, Oncorhynchus mykiss.

무지개송어(Oncorhynchus mykiss)간세포배양에 있어서 Ouabain에 의한 세포내 고Calcium 농도가 Vitellogenin 합성에 미치는 효과

  • Published : 1998.05.01
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Abstract

Effects of high concentration of intracellular calcium on estradiol-induced vitellogenin(VTG) induction were examined using ouabain in Primary hepatocyte culture in the rainbow trout Oncorhynchus mykiss. Ouabain increases cytosolic free calcium as a result of inhibition of $Na^+ - Ca^{2+}$ exchanger. Ouabain markedly reduced VTG production to the control level, despite of calcium concentrations in the incubatin medium. Therefore, ouabain would reduce VTG production not by increasing intracellular calcium bt directly by inhibiting $Na^+ - K^+$ ATPase.

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References

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