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Effect of Embryo Number and Incubation Volume on the Development of Pre- and Post-implantation Mouse Embryos In Vitro (배아밀도와 배양액 용량이 착상전후의 생쥐배아의 체외 성장에 미치는 영향)

  • Kang, Byung-Moon;Cheon, Yong-Pil;Kim, Ji-Young;Kim, Jeong-Hee;Lee, Ji-Yun;Chae, Hee-Dong;Kim, Chung-Hoon;Chang, Yoon-Seok;Mok, Jung-Eun
    • Clinical and Experimental Reproductive Medicine
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    • v.24 no.3
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    • pp.377-383
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    • 1997
  • The effects of embryo number and incubation volume on the development of mouse embryos were evaluated. The growth rate of two-cell mouse embryos to attached blastocyst stage and the growth rate of blastocysts to early somite stage were assessed after culture in different incubation volumes and embryo densities. Embryos were collected from ICR female mice superovulated with pregnant mare serum gonadotropin and human chorionic gonadotropin and mated by ICR males. In experiment 1, groups of one, five, ten, twenty 2-cell embryos were cultured in 10-, 50-, 500-, 1000-${\mu}l$ drops of BWW media under mineral oil at $37^{\circ}C$ in a humidified atmosphere of 5% $CO_{2}$ and 95% air. As the incubation volume decreased, significantly (p<0.05) higher rates of embryos reached morular and blastocyst stage on day 3 and 4 culture, respectively. In experiment 2, groups of one, five, ten, twenty blastocysts were cultured in 1- and 2-ml volumes of CMRL 1066 media under same condition as in experiment 1. However the reverse was the result. Decreasing the number of embryos incubated per volume from 1 to 20 significantly (p<0.05) increased the number of blastocysts reaching the late egg cylinder (LEC) and early somite (ES) stage on day 6 and 8 culture, respectively, regardless of incubation volume. Blastocysts cultured in 2ml had higher (p<0.05) development rates to LEC and ES stage on day 6 and 8 culture, respectively, than embryos cultured in 1ml. Our results suggest that the effects of embryo number and incubation volume on the development of mouse embryos are stage specific and the shifting point was between hatching and EEC stage.

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The Impact of Population Aging on Energy Use and Carbon Emissions in Korea (인구 고령화가 에너지 사용과 탄소 배출에 미치는 영향)

  • Kim, Dong Koo;Park, Sunyoung
    • Journal of Environmental Policy
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    • v.13 no.2
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    • pp.99-129
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    • 2014
  • This research estimates the impact of population aging on energy use and carbon emissions by energy sources and by industrial sectors in Korea until 2035. For the estimation, the structural change in household consumption expenditure identified by the age-specific consumption pattern was analyzed in conjunction with energy and environment input-output tables. The estimation result presents that, despite the population aging, energy use and carbon emissions induced by household consumption continue to increase until 2026, and then that elevated levels of energy use and carbon emissions will be maintained for a considerable period of time. According to the estimation by energy sources, the use of natural gas will show substantial increase while the use of crude oil will switch to a downturn at a relatively early period. According to the estimation by industrial sectors, carbon emissions in the sectors with relatively high consumption share of old households such as medical health, dwelling, lighting, heating, air-conditioning, and food will have substantial increase, whereas those in the sectors associated with education, transport, catering, and accommodation services will turn downward relatively early. In addition, the study analyzes through policy simulation the impact of aging-related policy similar to the basic pension system, which is recently being discussed for legislation, on energy use and carbon emissions.

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A Study on the Correlation and Concentration in Volatile Organic Compounds(Benzene, Toluene, Xylene) Levels According to the Indoor/Outdoor and the Type of Residents' House in Industrial Area (공단지역 일부 주민들의 주택유형 및 실내/외에 따른 VOCs(Benzene, Toluene, Xylene) 농도 및 상관성에 관한 연구)

  • Lee, Che-Won;Jeon, Hye-Li;Hong, Eun-Ju;Yu, Seung-Do;Kim, Dae-Sun;Son, Bu-Soon
    • Journal of Environmental Health Sciences
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    • v.36 no.5
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    • pp.351-359
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    • 2010
  • The objectives of this study were to understand the characteristics of residents in industrial areas and factors affecting exposure to the Volatile Organic Compounds(VOCs : Benzene, Toluene, Xylene) as well as to assess exposure levels according to house-type, and whether residents were indoors or outdoors. This research was designed to assess the differences in exposure levels to indoor, outdoor and personal VOCs in a case group and a control group across all areas, as well as in each different area, from May to October 2007, in. 110 residents of the G, Y and H industrial areas of the Jun-nam province. The geometric mea-levels of airborne benzene for the case group 1.31part per billion(ppb) indoor, 1.29 ppb outdoor, and 1.32 ppb for personal exposure were significantly higher than for the control group 0.99, 0.87 and 0.57 ppb, respectively. The geometric mean level for toluene personal exposure across the G, Y and H areas was 5.70 ppb for the case group and 6.31 ppb for the control group. While the outdoor level was 4.27 ppb for the case group and 5.06 ppb for the control group, The indoor level for the case group was 4.78 ppb, similar to that of the control group 4.69 ppb. The geometric mean levels for airborne xylene across the G, Y and H areas were 0.16 ppb(outdoor), 0.12 ppb(personal exposure) and 0.10 ppb(indoor) for the case group, and for the control group were 0.17(personal exposure) and 0.09 ppb(indoor and outdoor). The indoor/outdoor(I/O) ratio for case group is 1.19, while that of the control group is 1.15, indicating that the indoor level was higher than the outdoor level. The interrelationship differences among the three different types of levels in the air in the G, Y and H areas are statistically significant, except for the difference between the indoor and outdoor figures for xylene. In terms of the different types of houses and energy type uesd, the geometric mean level for airborne benzene, toluene and xylene for houses were 1.61, 5.39 and 0.12 ppb, respectively. while the figures for flats were 0.67, 3.32 and 0.05 ppb, respectively. Outdoors, the levels of benzene and toluene in flats were 0.71 and 2.62 ppb, respectively. and 1.58 and 5.35 ppb in houses. For personal exposure, the house levels of benzene, toluene and xylene were all higher than for flats. Houses using oil for heating have significantly higher levels than flats, which use gas for heating.

Spawning Behavior and Development of Eggs and Larvae of the Korea Freshwater Goby, Rhinogobius brunneus (Gobiidae: Perciformes) (한국산 밀어 (Rhinogobius brunneus)의 산란습성, 난발생 및 자어의 형태발달)

  • HAN Kyeong-Ho;KIM Yong Uk;CHOE Kyu-Jung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.31 no.1
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    • pp.114-120
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    • 1998
  • The spawning behavior, development of eggs and larvae of the Korea freshwater goby, Rhinogobius brunneus (Temminck et Schlegel) were studied. The eggs were spawned as a one-layer mass, hanging from the underside of a small pebble, and guarded by one male. The eggs were elliptic, about 1.48 mm in length and 0.65 mm in breadth, with a round top and a somewhat flat base with glutinous fibers. Hatching in the indoor tank with $17.0^{\circ}C$ in mean water temperature started from the 146 hours after fertilization. In the late developing stages, the embryo moved and freely changed its head to face the free tip of the chorion (normal egg) or to the basal end with a boundle of adhesive filaments (agrippa egg). Newly-hatched larvae were $3.10\~3.30$ mm in total length (mean: 3.22 mm), and mouth and anus were not yet open. Melanophores were present on the air- bladder, around the anus, and on the ventral part of the caudal region. The larva $3\~4$ days old transformed to postlarval stage, and they were $3.30\~3.85$ mm in total length (mean: 3.60 mm). As yolk sac and oil globules werw nearly absorbed, mouth and anus were open, and they fed rotifers actively. In $20\~22$ days after hatching the larvae grew to 5.85 mm in 71, and the caudal notochord flex at $45^{\circ}$.

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Selective Nitrate Removal Performance Analysis of Ion Exchange Resin in Shipboard Waste Washwater by Air Pollution Prevention Facility (선박용 대기오염장치 폐세정수 내 질산염의 선택적 제거를 위한 이온교환수지 공정 성능 평가)

  • Kim, Bong-Chul;Yeo, In-Seol;Park, Chan-Gyu
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.41 no.4
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    • pp.399-404
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    • 2021
  • From 1 January 2020, the limit for Sulphur in fuel oil used on board ships operating outside designated emission control areas will be reduced to 0.5 %. This regulation by international maritime organization (IMO) is able to significantly reduce the amount of Sulphur oxides (SOx) discharging from ships and should have environmental advantages and health for all over the world. To meet the regulation, in these days, wet scrubber system is being actively developed. However, this process leads to make washing wastewater. In this study, we evaluated ion exchange resin system in accordance with scrubber wastewater discharge regulation by IMO. Theoretical wastewater used as feed solution of lab scale water treatment systems. The results revealed that nitrate ion was removed selectively in spite of high TDS wash wastewater solution depending on ion exchange resin property. Moreover, it was possible to improve efficiency of the system by optimizing operating conditions.

Determination of Correlation between Cetane Number, Derived Cetane Number and Cetane Index for Diesel Fuel (경유연료의 세탄가, 유도세탄가 및 세탄지수의 상관관계 분석)

  • Jeon, Hwayeon;Kim, Ji Yeon;Kim, Shin;Yim, Eui Soon
    • Journal of the Korean Applied Science and Technology
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    • v.35 no.4
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    • pp.1134-1144
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    • 2018
  • Cetane Number is one of the quality standard for diesel, which assesses the compatibility of ignition quality of diesel compression in diesel engines. Cetane number must be upper 52 to keep the recent diesel quality standards. It is known that if cetane number is high, there will be shorter ignition delay periods than being lower. On the other hands, if cetane number is too high that exceeds the quality standard, there will increase the air pollution and decrease of the fuel efficiency because incomplete combustion. In South Korea, various methods are being used to measure the cetane number such as cetane number that used CFR engine, cetane index from calculate density and distillation temperature and derived cetane number to make up for CFR engine that ignition delay in high temperature is implemented. In this study will be conducted by collecting the diesel from the major oil companies, and try to analyze the correlation between the different methods of cetane number with various factors. At the results of this study, it was shown that the cetane index is high then cetane engine and derived cetane number. therefore it will be necessary to additional research for out of cetane number quality standards.

Study on Characteristics of Change of Physical/Chemical Property in Domestic Aviation Fuel by the Quality Monitoring Analysis (국내 항공유(Jet A-1) 품질모니터링을 통한 물성 변화 특성 연구)

  • Doe, Jin-woo;Youn, Ju-min;Jeon, Hwa-yeon;Yim, Eui-soon;Lee, Joung-min;Kang, Hyung-kyu
    • Journal of the Korean Applied Science and Technology
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    • v.35 no.4
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    • pp.1327-1337
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    • 2018
  • Aviation fuel oil is more strictly controlled than other transport fuels because it can lead to major accidents in the event of a problem. The quality standards of the aircraft are specified by the domestic Korean Standard, the American Society for Testing and Materials and the International Air Transport Association, respectively. From 2016 to 2017, the quality analysis of 6 items such as aromatic content, sulfur content and distillation characteristics was carried out on the jet fuel produced at five domestic refineries. Domestic production of jet fuel has been shown to be in conformity with the quality standards and has been maintained at a constant level throughout the year. Compared with the specification of ASTM and IATA the aromatic content of domestic KS specification is set to be strictly 1.5 wt% higher than the ASTM and IATA setting specification, but it satisfies this specification sufficiently. In addition, other items such as sulfur content, distillation property and flash point satisfied both domestic and international specification.

Synthesis and Characterization of Interfacial Properties of Sorbitan Laurate Surfactant (Sorbitan Laurate 계면활성제 합성 및 계면 특성에 관한 연구)

  • Lee, Seul;Kim, ByeongJo;Lee, JongGi;Lim, JongChoo
    • Applied Chemistry for Engineering
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    • v.22 no.1
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    • pp.37-44
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    • 2011
  • The critical micelle concentration (CMC) of sorbitan laurate SP 20 surfactant in this paper was near $7.216{\times}10^{-4}mol/L$ and the surface tension at CMC was about 26.0 mN/m, which showed higher CMC and lower surface tension than those of octylphenol ethoxylate octylphenol ethoxylate (OPE) 10 surfactant. Dynamic surface tension measurement using a maximum bubble pressure tensiometer showed that the adsorption rate at the interface between air and surfactant solution was found to be slower with SP 20 surfactant, presumably due to a low mobility of SP 20 surfactant monomer. The contact angle of SP 20 surfactant solution was observed to decrease with an increase in surfactant concentration and showed a larger value than that of OPE 10 surfactant solution. Half-life time for foams generated with 1 wt% surfactant solution was also larger with SP 20 surfactant, which indicated higher foam stability with SP 20 surfactant. Dynamic behavior study reveals that the solubilization of n-decane oil was much lower with SP 20, which is in good agreement with experimental results of foam stability, contact angle and CMC. Dynamic interfacial tension measurement by a spinning drop tensiometer shows that interfacial tensions at equilibrium condition in both systems were almost the same but the time required to reach equilibrium was longer with SP 20.

Improvement of Optimal Bus Headway for Intermodal Transfer Station (교통수단간 연계를 위한 최적 버스 배차간격 조정 알고리즘 개발)

  • Ryu, Byoungyong;Yang, Seungtae;Bae, Sanghoon
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.29 no.1D
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    • pp.17-23
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    • 2009
  • Due to the rapid increase of vehicles on the street, Korean society is facing worsening traffic congestions and air pollutions. Also, the oil price pickup has led to increasing need for the use of public transportation. In particular, transfering among public transportation may be a main factor for riders who are commuting for a long distance journey. In order to ensure such connectivity, transfer stations have been actively built in Korea. However, it would be necessary to shift those vehicles, from cars to public transportations by enhancing the users' satisfaction with public transportation through strategies for minimizing the users' waiting cost by building an efficient connective system between transportation modes as well as the preparation of aforementioned transfer stations. Therefore, this study aimed to develop an algorithm for minimizing transferring passengers' waiting costs based on service intervals of linked buses within the transfer facilities. In order to adjust the service interval, we calculated the total costs, involving the wait cost of transfer passengers and bus operation costs, and produced an allocation interval, that would minimize the costs. We selected a KTX departing from Seoul station, and a No. 6014 bus route in Gwangmyeong city where it starts from the Gwangmyeong station in order to for verifying the model. Then, the transfer passengers' total waitting cost was reduced equivalent to the maximum of 212 minutes, and it revealed that the model performed very effectively.

Changes of Physical Properties of Soils by Organic Material application (유기성 물질 시용에 따른 농경지 토양물리성 변화 연구)

  • Kim, Lee-Yul;Cho, Hyun-Jun;Han, Kyung-Hwa
    • Korean Journal of Soil Science and Fertilizer
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    • v.37 no.5
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    • pp.304-314
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    • 2004
  • The objective of this study was to investigate the effect of organic materials (compost, straw, green manure, pig manure, seed production oil cake, and industrial by products including municipal sewage sludge, industrial sewage sludge, leather processing sludge, and alcohol fermentation processing sludge) on physical properties of soils in seven paddy and four upland fields with differential soil textures, sandy loam, loam, or clay loam, etc. The investigated physical parameters were bulk density (BD), air permeability (AP), macroporosity, hardness, shear resistance, frictional resistance, water stability aggregate (WSA), and Middleton's dispersion ratio. Except for coarse sandy loam field with weak structure, a decrease in BD and shear resistance, and an increase in macroporosity and AP in plots with applying organic materials compared to plots without applying organic materials appeared. In upland fields, the positive effect of organic materials on WSA, BD, and air permeability was higher than in paddy fields. The combined plot of NPK and compost had lower BD, hardness, and shear resistance, and higher macroporosity and WSA than plot with compost. Green manure had higher positive effect on physical properties of soils compared to other organic materials and the extent of positive effect had no significant correlation with soil organic matter content. Of industrial byproducts applied in coarse sandy loam soil under upland condition, municipal sewage sludge and pig manure compost had higher effect on increase of WSA than leather processing sludge and alcohol fermentation processing sludge. Unlike WSA, there were no significant differences between industrial byproduct types in other physical properties. in silty clay loam soil under the upland condition, straw had more positive effect on soil physical parameters than hairy vetch and pig manure. Therefore, different organic materials had differently active effect on physical parameters depending on types of soil and land use. Especially, it could be thought that well-decomposed organic materials have the advantage of an increase in organic matter content, while coarse organic materials of an increase in WSA.