• 대한수의학회지
• /
• 제39권1호
• /
• pp.220-225
• /
• 1999

The aim of these experiments was to investigate the effects of bovine oviduct epithelial cells (OEC) derived from different segments to bind sperm binding and maintain their motility in vitro. In experiment 1, the number of sperm attached to OEC derived from isthmus or ampulla, the motility of unattached sperm during co-culture and fertilizing ability were assessed. In experiment 2, heparin treated sperm (hsp) or no treated sperm (nsp) were used to evaluate OEC binding ability of capacitated sperm. In experiment 1, regardless of their origin, approximately 65% of the sperm were attached to OEC within 2h. From 6h of co-culture, the numbers of unattached sperm on ampullary OEC were significantly higher than those on isthmic OEC (p<0.005). From 12h of co-culture, the motility of unattached sperm on isthmic OEC were significantly higher than those on ampullary OEC(p<0.05). The cleavage rate of oocytes inseminated on OEC derived from isthmic segment was also significantly higher than those from ampullary segment (p<0.01). In experiment 2, the numbers of unattached hsp on OEC were significantly higher than those of controls (p<0.01), between 2-24h examination. From 12h of co-culture, the motility of unattached nsp were significantly greater than those of hsp (p<0.01). These results show that bovine OEC derived from the isthmus play more important role(s) for sperm binding, maintaining motility and fertilization in vitro than those from the ampulla, and heparin induced capacitation may change sperm binding ability on OEC in vitro.

• 대한기계학회논문집B
• /
• 제28권2호
• /
• pp.160-166
• /
• 2004

Combustion using oxygen enriched air is known as a technology which can increase flame stability as well as thermal efficiency due to improving the burning rate. Lift-off, blowout limit and flame length were examined as a function of jet velocity, coflow velocity and OEC(Oxygen Enriched Concentration). Blowout limit of the flame below OEC 25% decreased with increase of coflow velocity, but the limit above OEC 25% increased inversely. Lift-off height decreased with increase of OEC. In particular, lift-off hardly occurred in the condition above OEC 40%. Flame length of the flames above OEC 40% was increased until the blowout occurred. Great flame stability was obtained since lift-off and blowout limit significantly increased with increase of OEC.

• 대한기계학회:학술대회논문집
• /
• 대한기계학회 2003년도 추계학술대회
• /
• pp.326-331
• /
• 2003

Combustion using oxygen enriched air is known as a technology which can increase flame stability as well as thermal efficiency due to improvement of the burning rate. Lift-off, blowout limit and flame length were examined as a function of jet velocity, coflow velocity and OEC(Oxygen Enriched Concentration). Blowout limit of the flame below OEC 25% decreased with coflow velocity, but the limit above OEC 25% increased inversely. Lift-off height decreased with increase of OEC. Especially lift-off hardly occurred in the condition above OEC 40%. Flame length of the flames above OEC 40% was increased until the blowout occurred. Flame stability became improved since lift-off and blowout limit increased much with increase of OEC.

• 한국수정란이식학회지
• /
• 제13권3호
• /
• pp.227-233
• /
• 1998

난관상피세포와 그 배양액에서 분비된 고분자 분획이 소정자의 수정능력에 어떠한 영향을 미치는지 알아보고자 실시한 실험에서 다음과 같은 결론을 얻었다. 1. 난관상피세포배양액으로부터 MW 5 kDa cut-off bucket을 사용하여 탈염 및 농축을 실시, 단백질/고분자분획을 회수하고 이를 체외수정용 배양액에 첨가하고 난관상피세포 monolayer와 공배양을 통해 체외수정을 실시한 결과 고분자분획첨가 및 난관상피세포 공배양(OM+OEC)군이 고분자분획첨가(OM) 군에 비하여 유의적으로 높은 분할율을 나타내었으며 난관상피 세포 공배 양(OEC)군 및 OM 군 모두 대조군에 비해서는 유의적으로 높은 분할율을 나타내었다(p〈0.01). 2. 난관상피세포와 전배양한 정자의 체외수정능을 조사한 결과 정자와 OEC를 4시간 동안 전배양한 후 체외수정한 군이 난자와 정자를 동시에 배양한 군에 비하여 유의적으로 높은 분할율을 나타내었다(p〈0.01). 이상의 결과에서 소 정자의 체외수정능은 난관 상피세포와의 접촉 및 분비액유래 고분자단백분획의 공동효과에 의한 것으로, 난관상피세포와의전배양은 체외에서 수정능획득을 유도하는 것으로 판단된다.

• Rapid Communication in Photoscience
• /
• 제3권2호
• /
• pp.35-37
• /
• 2014

PEC (photoelectrochemical) water splitting for $O_2/H_2$ production is one of the promising but difficult way to utilize solar energy. Among photocatalytic materials for PEC water oxidation, $BiVO_4$ (Eg = 2.4 eV) has been recently intensively studied since it has various advantageous properties. But its maximum efficiency has not been realized owing to kinetic factors - slow water oxidation at surface & insufficient stability. These problems can be simultaneously solved by application of oxygen evolution catalyst (OEC) such as $CoO_x$, Co-Pi, $IrO_x$ etc. Herein we report the first successful application of $NiFeO_x$ OEC on $BiVO_4$, showing good performance compared to other effective OEC applied on $BiVO_4$ under basic conditions. The enhanced activity of OEC loaded $BiVO_4$ has been supported by the surface charge separation efficiency and electrochemical impedance studies.

• 대한기계학회논문집B
• /
• 제28권2호
• /
• pp.223-229
• /
• 2004

Combustion using oxygen enriched air is known as a technology which can increase thermal efficiency due to increase of the flame temperature. Flame shapes, schlieren photos, OH radical chemiluminescence and local flame temperature were examined as a function of OEC(Oxygen Enriched Concentration) in a coaxial non-premixed jet. With increase of OEC, flame length and width decreased, but its brightness increased significantly, and the size of vortices in the flame also increased. Especially, the reaction around the flame surface became active. The strong OH intensity appeared to be made and moved from middle stream to upper one with increase of OEC, which shows combustion reaction in the upper stream becomes more dominant In addition, the temperature distributions of the flames showed similar tendency with OH radical intensities. A flame with high temperature and strong stability was obtained with increasing OEC of the coflow.

• 한국정보과학회:학술대회논문집
• /
• 한국정보과학회 2012년도 한국컴퓨터종합학술대회논문집 Vol.39 No.1(A)
• /
• pp.113-115
• /
• 2012

안드로이드 앱의 불법 복제를 방지하고자 기존에 온라인 실행(Online Execution Class: OEC) 또는 앱을 암호화된 상태로 배포하여 실행 시에 적법한 사용자만이 암호를 복호화하여 실행이 가능하도록 하는 방법들이 있다. 하지만 스마트폰이 저전력과 저사양인 점을 고려할 때, 위에 방법은 스마트폰의 보안을 보장할 수 있지만 성능 면에서 부담이 된다. 본 논문에서 OEC를 암호화하여 보안 수준을 높이고 개발자에게 보안 수준을 선택할 수 있게 하여 환경에 맞추어 안전을 유지할 수 있도록 시스템 설계를 제안한다.

• 자원ㆍ환경경제연구
• /
• 제15권2호
• /
• pp.201-245
• /
• 2006

본 연구는 일반균형모형을 이용하여 배출권 거래로 인한 일반균형효과와 온실가스 저감비용의 효율성에 대해 알아보았다. 모든 국가가 교토의정서를 비준하고 연합을 형성한 국가끼리 배출권 거래를 한다는 가정 하에서 분할함수형태의 온실가스저감게임을 구성하였고, 전지구를 미국과 미국 이외의 OECD 국가군, 구소련연방, 개도국으로 나누어 연합구조별로 각 국가군에 미치는 영향을 일반균형모형으로 분석한 결과 대연합 하에서 기후변화협약은 연합을 형성하지 않은 ${\phi}$연합구조에 비해 동일한 환경적 효과를 달성하는데 978억 달러를 줄일 수 있어서 효율적인 것으로 나타났다. 하지만, 이 중 약 절반을 미국이 가지고 OEC가 20%를 가지는 반면 배출권 판매국인 구소련연방과 개도국은 각각 18%와 10%만을 가지는 것으로 나타나 배출권 구매국이 전체 연합이익의 약 70%를 가지는 것을 확인하였다. 개도국이 연합에 참여하지 않으면 2010년 Annex-I 국가의 저감량의 최대 22%를 상쇄하는 유출량을 발생하므로 기후변화협약의 본래의 목적인 온실가스 저감 및 안정화를 위해서는 개도국이 연합에 참여하여 유출량을 줄여야 한다. 하지만, 전지구적 배출권 거래시 Annex I 국가가 상당량의 연합이익을 가져가고 개도국의 에너지 산업은 위축되므로 기후변화협약의 안정성을 위해 추가지원금을 제안하고 개도국을 연합에 참여하도록 유인할 필요가 있다.

• Molecules and Cells
• /
• 제41권6호
• /
• pp.582-590
• /
• 2018

Endothelial progenitor cells (EPCs) and outgrowth endothelial cells (OECs) play a pivotal role in vascular regeneration in ischemic tissues; however, their therapeutic application in clinical settings is limited due to the low quality and quantity of patient-derived circulating EPCs. To solve this problem, we evaluated whether three priming small molecules (tauroursodeoxycholic acid, fucoidan, and oleuropein) could enhance the angiogenic potential of EPCs. Such enhancement would promote the cellular bioactivities and help to develop functionally improved EPC therapeutics for ischemic diseases by accelerating the priming effect of the defined physiological molecules. We found that preconditioning of each of the three small molecules significantly induced the differentiation potential of $CD34^+$ stem cells into EPC lineage cells. Notably, long-term priming of OECs with the three chemical cocktail (OEC-3C) increased the proliferation potential of EPCs via ERK activation. The migration, invasion, and tube-forming capacities were also significantly enhanced in OEC-3Cs compared with unprimed OECs. Further, the cell survival ratio was dramatically increased in OEC-3Cs against $H_2O_2$-induced oxidative stress via the augmented expression of Bcl-2, a pro-survival protein. In conclusion, we identified three small molecules for enhancing the bioactivities of ex vivo-expanded OECs for vascular repair. Long-term 3C priming might be a promising methodology for EPC-based therapy against ischemic diseases.

• Asian Spine Journal
• /
• 제12권6호
• /
• pp.998-1009
• /
• 2018

Study Design: Olfactory ensheathing cells (OECs) from rat olfactory mucosa were cultured, characterized, and transplanted into a rat model of spinal cord injury (SCI). Purpose: To evaluate different doses of OECs in a rat model of SCI. Overview of Literature: SCI causes permanent functional deficit because the central nervous system lacks the ability to perform spontaneous repair. Cell therapy strategies are being explored globally. The clinical use of human embryonic stem cell is hampered by ethical controversies. Alternatively, OECs are a promising cell source for neurotransplantation. This study aimed to evaluate the efficacy of different doses of allogenic OEC transplantation in a rat model of SCI. Methods: OECs were cultured from the olfactory mucosa of Albino Wistar rats; these cells were characterized using immunohistochemistry and flow cytometry. Rats were divided into five groups (n=6 rats each). In each group, different dosage ($2{\times}10^5$, $5{\times}10^5$, $10{\times}10^5$, and >$10{\times}10^5$) of cultured cells were transplanted into experimentally injured spinal cords of rat models. However, in the SCI group, only DMEM (Dulbecco's modified Eagle's medium) was injected. Rats were followed up upto 8 weeks post-transplantation. The outcome of transplantation was assessed using the Basso, Beattie, Bresnahan (BBB) scale; motor-evoked potential studies; and histological examination. Results: Cultured cells expressed 41% of p75NTR, a marker for OEC, and 35% of anti-fibronectin, a marker for olfactory nerve fibroblast. These cells also expressed $S100{\beta}$ and glial fibrillary acid protein of approximately 75% and 83%, respectively. All the transplanted groups showed promising BBB scores for hind-limb motor recovery compared with the SCI group (p<0.05). A motor-evoked potential study showed increased amplitude in all the treated groups compared with the SCI. Green fluorescent protein-labeled cells survived in the injured cord, suggesting their role in the transplantation-mediated repair. Transplantation of $5{\times}10^5$ cells showed the best motor outcomes among all the doses. Conclusions: OECs demonstrated a therapeutic effect in rat models with the potential for future clinical applications.