• Restorative Dentistry and Endodontics
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• v.29 no.4
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• pp.399-408
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• 2004

Odontoblasts are responsible for the formation and maintenance of dentin. They are known to synthesize unique gene products including dentin sialophosphoprotein (DSPP). Another unique genes of the cells remain unclear. OD314 was isolated from the odontoblasts/pulp cells of rats and partially characterized as an odontoblast-enriched gene (Dey et al., 2001). This study aimed to elucidate the biological function of OD314, relating to odontoblast differentiation and dentinogenesis. After determining the open reading frame (ORP) of OD314 by transient transfection analysis using green fluorescent protein (GPP) expression vector, mRNA in-situ hybridization, immunohistochemistry, reverse transcription-polymerase chain reaction (RT-PCR) and western analysis were performed. The results were as follows: 1. In in-situ hybridization, OD314 mRNAs were expressed in odontoblasts of developing coronal and root pulp. 2. OD314 was a novel protein encoding 154 amino acids, and the protein was mainly expressed in cytoplasm by transient transfection analysis. 3. Mineralized nodules were associated with multilayer cell nodules in the culture of human dental pulp cells and first detected from day 21 using alizarin-red S staining. 4. In RT-PCR analysis, OD314, osteocalcin (OC) and DSPP strongly expressed throughout 28 days of culture. Whereas, osteonectin (ON) mRNA expression stayed low up to day 14, and then gradually decreased from day 21. 5. Western blots showed an approximately 17 kDa band. OD314 protein was expressed from the start of culture and then increased greatly from day 21. In conclusion, OD314 is considered as an odontoblast-enriched gene and may play important roles in odontoblast differentiation and dentin mineralization.

• Journal of Korean Society of Transportation
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• v.33 no.2
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• pp.113-124
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• 2015

The KOTI(Korea Transport Institute) released the new version of KTDB(Korea Transport DataBase) in public. The new KTDB is different from the past KTDB in using the concept of trip generation and trip attraction instead of using the concept of Origin-Destination (OD), which was used in the past KTDB. Thus, the appropriate analysis method for future travel demand became necessary for the new type of KTDB. The method should be based on the concept of PA(Production-Attraction). This study focused on analysis of trip generation and trip distribution related to newly generated trips by future land developments. The study also described clearly the standardized forecasting process and methods with PA travel tables. The study showed that the analysis results with OD-based analysis can be different from the results with PA-based analysis in forecasting travel demand for a simple example case even though they used exactly same orignal travel data. Therefore, this study emphasized that a proper method should be applied with the new PA-based KTDB. It is necessary to prepare and disseminate guidelines of the proper forecasting method and application with PA-based travel data for practician.

• Journal of the Korean Applied Science and Technology
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• v.38 no.3
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• pp.629-637
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• 2021

To develop a safer cosmetic preservative, we carried out a comparative study on characteristics of OD and OD-gal, where OD-gal was synthesized from OD using E. coli β-gal. OD-gal synthesis was confirmed by mass spectrometry analysis as sodium adduct ion (m/z=331.1731) and protonated ion (m/z=309.1926) of OD-gal. To compare the antimicrobial activities of OD and newly synthesized OD-gal, MIC values were investigated using E. coli, S. aureus, C. albicans, and A. niger. As a result, it was observed that there was no remarkable difference between MIC values of OD and OD-gal. In addition, to compare the cytotoxicity of OD-gal and OD, HaCaT cells were treated with OD or OD-gal, and then cell viability was quantified using EZ-Cytox assay. In the case of 1.5% OD, the cell viability was 64% at 24 h and 42% at 48 h compared to the control, and cell viability of 1.5% OD-gal-treated cells showed no significant change at 24 h and was 85% at 48 h. Consequently, the cytotoxicity of OD-gal-treated cells was reduced by more than 40% when compared with that of OD-treated cells. Thus, the newly synthesized OD-gal in this study is safer than the existing OD used as a cosmetic additive. In the future, OD-gal will be applicable as a substitute for OD as a less toxic preservative for the cosmetic industry.

• Journal of Korean Society of Transportation
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• v.18 no.6
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• pp.89-99
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• 2000

To cope with the limits of conventional O-D trip matrix collecting methods, several approaches have been developed. One of them is bilevel Programming method Proposed by Yang(1995), which uses Sensitivity Analysis Based(SAB) algorithm to solve Generalized Least Square(GLS) problem. However, the SAB a1gorithm has revealed two critical short-comings. The first is that when there exists a significant difference between target O-D matrix and true O-D matrix, SAB algorithm may not produce correct solution. This stems from the heavy dependance on the historical O-D information, in special when gravel Patterns are dramatically changed. The second is the assumption of iterative linear approximation to original Problem. Because of the approximation, SAB algorithm has difficulty in converging to Perfect Stackelberg game condition. So as to avoid the Problems. we need a more robust and stable solution method. The main purpose of this Paper is to show the problem of the dependency of Previous models and to Propose an alternative solution method to handle it. The Problem of O-D matrix estimation is intrinsically nonlinear and nonconvex. thus it has multiple solutions. Therefore it is necessary to require a method for searching globa1 solution. In this paper, we develop a solution algorithm combined with genetic algorithm(GA) , which is widely used as probabilistic global searching method To compare the efficiency of the algorithm, SAB algorithm suggested by Yang et al. (1992,1995) is used. From the results of numerical example, the Proposed algorithm is superior to SAB algorithm irrespective of travel patterns.

• Journal of the Korea Convergence Society
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• v.10 no.12
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• pp.315-325
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• 2019

The purpose of this study is to design and construct a data mart that anyone can easily analyze subway OD movement patterns. Subway OD data of the year 2017 was downloaded from the Seoul Open Data Plaza and used as the source data. A multidimensional model was designed, and Gaussian mixed cluster analysis and visualization analysis using Tableau were performed. Interestingly, movement between suburban and Seoul accounts for 23% of the total traffic. The passengers of Suwon Station move to the suburbs much more than Seoul, while Pangyo Station mostly moves to Seoul. As a result of Gaussian mixed cluster, eight clusters of OD segments were found, and the characteristics of each cluster were characterized by segment distance and passenger size.

• Journal of the Korean Society for Railway
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• v.19 no.5
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• pp.656-662
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• 2016

Based on time dependent location data of mobile phone users, users' ODs were produced after tracing their travel route and inducing their origins and destinations. System considered average signalizing frequency, which means that the longer the travel length is the more frequent the signal is. This is a home-based OD and is limited to the Seoul Metropolitan area. The OD matrix from the mobile phone data which was aggregated to the cell and transformed to the 'Dong' area, was compared to the KTDB OD. The results can be analyzed and it was determined that they are highly correlated because individual coefficients are 0.98 and 0.85, the former between the OD of this study and the KTDB Si/Gun/Gu unit area OD and the latter between the OD of this study and the Dong unit area KTDB OD.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2013.05a
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• pp.205-207
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• 2013

In a situation that building energy consumption is increasing worldwide, the research utilizing BIM technology to analyze building energy has been actively conducted. On the other hand, data input method of the building energy analysis has been still manually entered. This paper proposed a improved input method of required information for building energy analysis using the ECO2-OD program. As a result, although some required information of BIM based design software could be almost entered when it comes to general information and architectural sector, it has a problem to be handled in HVAC sector. Therefore, in the both of general and architectural sectors, the BIM information from the BIM-based design software could be directly used to automatically and systematically input the information. Future research should be studied the algorism and method in connection with data exchange to utilize input method of ECO2-OD from BIM data.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.22 no.1
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• pp.219-232
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• 2023

Mobility services need to change according to the regional characteristics of the target service area. Accordingly, analysis of mobility patterns and characteristics based on Origin-Destination (OD) data that reflect travel behaviors in the target service area is required. However, since conventional methods construct the OD data obtained from the administrative district-based zone system, it is hard to ensure spatial homogeneity. Hence, there are limitations in analyzing the inherent travel patterns of each mobility service, particularly for new mobility service like Demand Responsive Transit (DRT). Unlike the conventional approach, this study applies a data-driven clustering technique to conduct spatial analyses on OD travel patterns of regional mobility services based on reconstructed OD data derived from re-aggregation for original OD distributions. Based on the reconstructed OD data that contains information on the inherent feature vectors of the original OD data, the proposed method enables analysis of the spatial characteristics of regional mobility services, including public transit bus, taxi and DRT.

• Journal of the Korean Applied Science and Technology
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• v.38 no.3
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• pp.824-831
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• 2021

1, 2-Octanediol galactoside (OD-gal) has been synthesized from 1, 2-octanediol (OD), as a safer cosmetic preservative, using recombinant Escherichia coli β-galactosidase (β-gal). To confirm the molecular structure of synthesized OD-gal, mass spectrometry and NMR (¹H- and ¹³C-) spectroscopy of OD-gal were carried out. In the reaction mixture, a sodium adduct ion of OD-gal (m/z=331.1732) was identified using mass spectrometry analysis. In addition, ¹H NMR spectrum of OD-gal showed multiple peaks corresponding to the galactosyl group, which is evidence of galactosylation on OD. Downfield proton peaks at δ_H 4.39 ppm and multiple peaks from δ_H 3.98~3.55 ppm were indicative of galactosylation on OD. Up field proton peaks at δ_H 1.52~1.26 ppm and 0.89 ppm showed the presence of CH₂ and CH₃ protons of OD. ¹³C NMR spectrum revealed the presence of 24 carbons suggestive of α- and β-anomers of OD-gal. Among 14 carbon peaks from each anomer, the 4 peaks at δ_C 31.4, 29.0, 22.3, and 13.7 ppm were assigned to be overlapped showing only 24 peaks out of a total of 28 peaks. The mass value from mass spectrometry analysis of OD-gal, and ¹H and ¹³C NMR spectral data were in good agreement with the expecting structure of OD-gal. Finally, we identified a galactose molecule from the hydrolysate of OD-gal using β-gal. We are expecting that through future study it will eventually be able to develop a safe cosmetic preservative.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.18 no.5
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• pp.100-111
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• 2019

The existing selected station analysis (SSA) method analyzes the link transfer mode data between origin and destination of individuals passing through stations from a microscopic standpoint. As such, existing SSA is insufficient as it uses integrated analysis using macroscopic data such as subway lines. This research builds a line chain OD based on path search of individual passenger's movement through the subway, and explores means to utilize the findings. First, a method is proposed that searches the traversed subway path from the linked passage modes that the passenger uses and applies the results to SSA line analysis. Compared to the existing SSA, this method provides for analysis of commonly conflicting features such as the line on which the station is passed, and the stations included on the line thanks to the presence of complete information of the individual passenger's traversed path. It also allows for integrated observation of the line chain OD that approaches a certain station. For enhanced understanding, Seoul Metro Line 9 is used as a case study to demonstrate the integrated formulation concept of line chain OD centered around a certain station as well as the macroscopic features of the traversed path that approaches stations included on the line.