• Journal of Korean Society on Water Environment
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• v.22 no.3
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• pp.566-571
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• 2006

A sequencing batch biofilm reactor (SBBR) operated with a cycle of anaerobic - aerobic - anoxic - aerobic has been evaluated for the nutrient removal characteristics. The sponge-like moving media was filled to about 10% of reactor volume. The sewage was the major substrate while external synthetic carbon substrate was added to the anoxic stage to enhance the nitrogen removal. The operational results indicated that maximum T-N and T-P removal efficiencies were 97% and 94%, respectively were achieved, while COD removal of 92%. The observations of significant nitrogen removal in the first aerobic stage indicated that nitrogen removal behaviour in this SBBR was different to conventional SBR. Although the reasons for aerobic nitrogen removal has speculated to either simultaneous nitrification and denitrification or anoxic denitrification inside of the media, further researches are required to confirm the observation. The specific oxygen uptake rate (SOUR) test with biofilm and suspended growth sludge indicated that biofilm in SBBR played a major role to remove substrates.

• Journal of Bio-Environment Control
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• v.28 no.4
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• pp.376-387
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• 2019

Melons are mostly grown in soil, but it is susceptible to damage due to injury by continuous cropping such as Fusarium wilt and root rot. Hydroponic cultivation system can overcome the disadvantages of soil cultivation with precise nutrition management and a clean environment. When using the coir substrate, the most environmentally friendly organic substrate used for hydroponics, it is analyzed how the growth and fruit quality of the melon depends on the ratio of chips and dust and the amount of irrigation. The purpose of this study was to provide the basic data of melon hydroponics when cultivated in spring. The two types of the coir substrates used in the experiments were chip and dust ratios of 3 :7 and 5 : 5 respectively. The substrate with high dust ratios had excellent physical characteristics, such as container capacity and total porosity, and the drainage EC level showed a high value of $3.0-6.8dS{\cdot}m^{-1}$. When the amount of irrigation is provided based on the drainage rate, the group provided the nutrient solution on the basis of 10% drainage supplied 91 L per plant, which was reduced by about 30% compared to the group with the highest water supply. In addition, the total drainage showed less than 10 L per plant with a minimum water supply and was reduced by 30 - 70% in substrate with a high dust rates. In substrate with high water supply and high dust ratio, leaf growth and fruit enlargement were good, and the soluble solids content varies greatly from cultivar to cultivar. If you provided the amount of irrigation based on 10% drainage rate, the fruit weight will be decreased, but the amount of irrigation can be reduced. Therefore, it is considered that managing the water & nutrient properly taking into account the characteristics of coir substrate and cultivar can produce melon of uniform quality using hydroponics.

• Journal of the Korea Organic Resources Recycling Association
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• v.29 no.1
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• pp.19-27
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• 2021

Hydroponic farming is a method to grow a plant without soil. Plants can be grown on water or hydroponic growing media, and they are fed with mineral nutrient solutions, which are fertilizers dissolved into water. Hydroponic farming has the advantage of increasing plant productivity over conventional greenhouse farming. Previous studies of hydroponic nutrient wastewater from acyclic hydroponic farms pointed out that hydroponic nutrient wastewater contained residual nutrients, and they were drained to a nearby river bank which causes several environmental issues. Also, previous studies suggest that excessive use of the nutrient solution and disposal of used hydroponic growing media and crop wastes in hydroponic farms are major problems to hydroponic farming. This study was conducted to determine the impact of hydroponic nutrient wastewater, used hydroponic growing media, and crop wastes from acyclic hydroponic farms on the surrounding environment by analyzing water quality and soil analysis of the above three factors. Three soil cultivation farms and several hydroponic farms in the Gangwon C region were selected for this study. Samples of water and soils were collected from both inside and outside of each farm. Also, a sample of soil and leachate from crop waste piles stacked near the farm was collected for analysis. Hydroponic nutrient wastewater from acyclic hydroponic farm contained an average of 402 mg/L of total nitrogen (TN) concentration, and 77.4 mg/L of total phosphate (TP) concentration. The result of TP in hydroponic nutrient wastewater exceeds the living environmental standard of the river in enforcement decree of the framework act on environmental policy by 993.7 times. Also, it exceeds the standard of industrial wastewater discharge standards under the water environment conservation act by 6~19 times in TN, and 2~27 times in TP. Leachate from crop waste piles contained 11,828 times higher COD and 395~2662 times higher TP than the standard set by the living environmental standard of the river in enforcement decree of the framework act on environmental policy and exceeds 778 times higher TN and 5 times higher TP than the standard of industrial wastewater discharge standards under the water environment conservation act. For more precise studies of the impact of hydroponic nutrient wastewater, used hydroponic growing media, and crop wastes from acyclic hydroponic farms on the surrounding environment, additional information regarding a number of hydroponic farms, arable area(ha), hydroponic farming area, seasonal, weather, climate factor around the river, and the property of the area and farm is needed. Analysis of these factors and additional water and soil samples are needed for future studies.

• Journal of Mushroom
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• v.16 no.4
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• pp.257-262
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• 2018

The aim of this study was to re-use spent mushroom substrate (SMS) with increased total nitrogen (T-N) and amino acid content and reduce the amount of cottonseed meal used as nutrient supplement in Pleurotus ostreatus cultivation. Bacteria used for improvement of the T-N content were GM20-4(Bacillus sp.) and Rhodobacter sphaeroides (RS). GM20-4 was isolated from the SMS of P. ostreatus and RS was obtained from Gwangjusi agricultural technology center. SMS in T1, T2, and T3 was reused as substrate after drying and the T-N content of dried SMS (D-SMS) was increased by 0.34% by treatment with the bacteria. T1 with 8% D-SMS and T2 with 18% D-SMS had higher rates of primordia formation compared with T3 and the control. The biological efficiency of the control and of treatment with 8%, 18%, and 26% D-SMS was 110%, 114%, 112%, and 79%, respectively. Considering the economic cost, yield, and biological efficiency, T2 with 18% D-SMS as the culture substrate for P. ostreatus was shown to be the most effective for cultivation.

• Journal of environmental and Sanitary engineering
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• v.16 no.3
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• pp.22-27
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• 2001

It have been investigated how algal and bacterial symbiotic reaction influences on removal of organic carbon in river ecosystem. And artificial experimentation apparatus was made for algae'and bacteia'culture as lab scale. Investigating and researching minutely the change of concentration of organic carbon substrate and the change of population density of algae'and of bacteria'with this artificial experimentation apparatus, the next results could be obtained. 1. Successful decrease of DOC(dissolved organic carbon) could not be expected unless algal and bacterial biomass floe was nut formed effectively and unless biosorption was not proceeded effectively in the very culture system in which artificial synthetic wastewater was supplied continuously at constant rate. 2. In conditions of culture liquid of 1335 glucnse mg/L(type 1) and of 267 glucose mg:L(type 2), the algal dominant species was always Chlorella vulgaris in both types in which artificial synthetic wastewater were supplied continuously at constant rate and algae population density was around maximum 107 cells/mL. 3. It was around 108 ~ 107 cells/mL that the population density of heterotrophic bacterium. In culture medium systems type 1 and type 2 in which artificial wastewater were supplied continuously at constant rate, the same density appeared initially when using the population density of Escherichia coli w 3110 as indirect indicator. And this density decreased rapidly till the culturing date 35 days were passed away, while this density increased with gentle slope after same date and then the trend of change at type 2 was more severe than one at type 1. 4. When seeing such a change of population density of Escherichia coli w 3110, the growth of heterotrophic bacterium appeared as survival instinct pattern of broader requirement of nutrient at condition of low concentration of organic carbon substrate than condition of high concentration of same substrate.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.26 no.4B
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• pp.433-437
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• 2006

Recently, mathematical modeling for the activated sludge process is important to design and control of wastewater treatment plant. Nevertheless, there is a lack of information regarding the pathway of substrate utilization between external and internal substrates in biological nutrient removal (BNR). In this research, a new activated sludge model (step growth model) is proposed and compare with ASM No.3. This model structure is consist of five processes; aerobic storage, growth on external substrate and stored intercellular storage compounds (ISCs), endogenous respiration and aerobic respiration of ISCs. The predicted results by the step growth model were more good accordance with the results of oxygen utilization rate (OUR) and TCOD experiment than that of the ASM No.3.

• Journal of Mushroom
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• v.17 no.3
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• pp.126-131
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• 2019

This study sought to identify the optimum substrate composition for the stable bottle cultivation of Sparassis latifolia. The main substrate was fermented larch sawdust. Six nutrient sources were mixed at a maximum volume ratio of 20%. The fresh weight of fruit body was the highest at 128.5 g for GMSL69033 and 126.6 g for 'Neoul' in the treatments of beet pulp and corn flour in a volume ratio of 15:5. In addition, the total cultivation period was 94 days, which was shorter than that required for other treatments. The selected substrate characteristics were pH 4.7, C:N (carbon to nitrogen) ratio of 106:4, moisture content of 70%, and air filling porosity of 38%. We plan to develop new income items through research on mycelial incubation and fruit body growth conditions.

• Proceedings of the Korean Geotechical Society Conference
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• 2000.03b
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• pp.331-337
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• 2000

When microorganism is injected into porous medium such as soils, biomass retained in the pore. Bacteria within these microcolonies produced large amounts of exopolysaccharides and formed a plugging biofilm. Soil pore size and shape are varied from the initial condition as a result of biofilm formation, which make hydraulic conductivity reduced and friction rate between soil aggregates increased. In this research, hydraulic conductivity reduction was measured after microorganism are inoculated and cultured with synthetic substrate and nutrient. Also, pore sand of before and after biofilm formation compared with scanning electron microscopy. Hydraulic conductivity of Sand and Poorly Graded Sand was decreased approximately 1/10∼1/100 after biomass inoculation and cultivation. Biofilm attached on soil aggregates is resistant to acidic or basic condition.

• KSBB Journal
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• v.14 no.5
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• pp.561-565
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• 1999

Optimum conditions for vacuum-drying ad cultivation of yeast cells for the production of active dry yeast were examined. At lower temperature, more drying time was required to dry the yeast pellet to reach the desirable water content(8%). Optimum temperature of vaccum oven and time for drying was 63$^{\circ}C$ and 90 min, respectively. Optimum medium composition for flask culture using cane molasses as the substrate were 0.25% sugar, 0.013% $K_2$HPO$_4$, 0.1% $K_2$HPO$_4$. and 0.125% (NH$_4$)$_2$SO$_4$. Culture temperature $25^{\circ}C$ gave the highest survival rate of dired yeast. After finishing fed-batch culture and the culture was left in the fermentor without adding any sugar or nutrient, survival of the dried yeast harvested from the fermentor increased to 86.0% after 36 hr. It was also observed that the yeast cells with higher budding rates showed lower survival rate.

• Proceedings of the Microbiological Society of Korea Conference
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• 2005.05a
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• pp.28-32
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• 2005

Epifluorescence microscopy and direct viable counting methods have shown that only 0.01-0.1% of all the microbial cells from marine environments form colonies on standard agar plates. To culture novel marine microorganisms, high throughput culturing (HTC) techniques were developed to isolate cells in very low nutrient media. This approaches was designed to address microbial metabolic precesses that occur at natural substrate concentrations and cell densities, which are typically about three orders of magnitude less than in common laboratory media. Approximately 5000 cultures of pelagic marine bacteria were examined over the course of 3 years. Up to 14% of cells from coastal seawater were cultured using this method, a number that is 1400 to 140-fold higher than obtained by traditional microbiological culturing techniques. Among the cultured organisms are many unique phylogenetic lineages that have been named as new phyla (7), orders (2, 5, 12), families (3), and genera (1, 4, 6). Over 90% of the cells recovered by this method do not replicate in standard agar plating, the most common method of microbial cell cultivation.