• Korean Journal of Soil Science and Fertilizer
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• v.45 no.6
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• pp.949-954
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• 2012

This study is conducted to evaluate the effects of chelating agents for improving plant growth and reusing accumulated nutrients in soils of plastic film house. Two experiments were carried out at follows: i) The incubation test was conducted using soils treated with 0, 300 mM of EDTA and DTPA to examine the availability of nutrients. ii) For the pot test, chinese cabbages were cultivated in soils with 0, 0.1, 0.5, 1, and 5 mM of EDTA and DTPA to examine the impacts of plant growth response. The application of chelating agents increased ther availability of soil nutrients in the following order: DTPA > EDTA > control. Inorganic concentration of chinese cabbages in DTPA treatments consderably increased in nitrogen, phosphate, iron and aluminium contents than that of the other treatments. The optimal concentration of DTPA for vigorous plant growth as 0.5 mM. Thus, DTPA was more effective than other chelating agents for healty growth of cabbages and the availability of nutrients accumulated in plastic film house.

• Korean Journal of Environmental Agriculture
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• v.17 no.4
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• pp.312-318
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• 1998

The potentials of some Korean wild plants as a phytoremediator for cleaning heavy metal pollution were measured. Several plant species, Ambrosia trifida, Brassica juncea, Rumex crispus, and Abutilon theophrasti screened previously for phytoremediator were treated with cadmium and copper solution. In order to know the growth response to heavy metal stress, the plants were cultivated in hydroponic system containing heavy metals with different concentration. To know the effects of heavy metals on emergence and seedling growth, seeds of 4 species were sown in the pot and watered with heavy metal solution adjusted pH to 6.5, 5.5, and 4.5. A proposed species as potential phytoremediator, A. trifida, showed tolerance to $20{\mu}mol/L$ Cd and $80{\mu}mol/L$ Cu in nutrient solution without apparent growth reduction, and up to $100{\mu}mol/L$ Cd and $400{\mu}mol/L$ Cu without critical visual injury. Up to 311mg/kg of Cd and 369mg/kg were accumulated in dried aerial part in A. trifida. In contrast, A. theophrasti showed injury at $400{\mu}mol/L$ Cu. Significant differences were shown in Cu accumulation among the four species. A. trifida had much higher concentrations of Cd in the shoot, whereas R, crispus accumulated higher concentrations of Cd in the shoot. Testing plant species showed reduced emergence rate with heavy metal treatment. When pH was lowered, the emergence and seedling growth were affected severely with heavy metal. We can suggested that A. trifida was the most proper species for phytoremediation in heavy metal-polluted regions.

• Journal of the Korean Society for Marine Environment & Energy
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• v.16 no.3
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• pp.189-201
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• 2013

To study characteristics of the water quality in the Hansan-Geoje bay, we analyzed the long term monitoring data collected at the two sites during the period of 1987~2010. The trophic state of the waters in Hansan-Geoje bay was the mesotrophic level by the classification of Wasmud et al.[2001]. The water nutrients increased steadily from a wet season (Aug.), it reached the maximum concentration peak in a dry season (Nov.), and then decreased steadily to the winter, it reached at the minimum value in May in the next year. The result of factor analysis divided the waters of Hansan-Geoje bay into the five factors (nutrient, season, inflow land water, pollution, internal production) and the factors represented the 76.82% on the status of the waters. According to time series analysis, temperature, DO and bottom DIP were increased, and pH and COD were decreased during the period of 1987~2010. In particular, the fluctuation trend of DIN has been turned from oversupply to shortage by the N/P ratio since before and after 1990's. The water quality of the Hansan-Geoje bay has been recovered except DIP since 1987, despite of its geographical characteristic which is a general semi-closesd bay and a massive aqua-culture ground. To preserve the waters in Hansan-Geoje bay, we need to know on the cause of the increase or accumulation of DIP, and we should continue to study on the interrelation between the aqua-culture and water environment.

• Horticultural Science & Technology
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• v.19 no.3
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• pp.342-347
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• 2001

The experiment was carried out to examine the effect of culture media and seawater on growth and mineral concentrations in Glasswort (Salicornia herbacea). Glassworts were grown in a greenhouse with 0, 10, 30 and 90% seawater and with or without nutrient media. The salinity tolerance affected on shoot height and Na, K and Ca concentrations significantly. The shoot height was increased with 10 and 30% seawater concentrations. At the higher seawater concentrations, more Na accumulated in a shoot was obserbed. The application of seawater concentration was effective on increasing Na, Mg, and Fe, concentrations and green leaf area rate and on decreasing K concentration. K/Na ratio of shoot was decreased at high seawater concentration, whereas Na concentrations was lowered at a low seawater concentrations. The mineral concentrations were changed according to the growing season. Na, K and Mg were high in June and Ca and P in September, respectively.

• Journal of the Korean Society for Marine Environment & Energy
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• v.7 no.1
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• pp.13-21
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• 2004

Temporal changes of Chl-α, physical and chemical factors were investigated by diurnal observation at 2-hour interval at three fixed stations in the western Chinhae Bay from 12 Aug. to 13 Aug. 1999. Difference of dissolved oxygen between surface and bottom layer was maximum when the thermocline were strong. Organic distribution such as COD was affected by the growth of phytoplankton. Limitting factor was nitrogen, that is, inorganic nitrogen plays a significant role on regulating the algal growth. Surface distribution of dissolved inorganic nitrogen was very low compared to bottom layer by uptake of organisms. Maximum value of Chl-α at station C2 and C11 were observed from subsurface layer, ranges of which exceeded possibility concentration of red tide outbreak, 10 mg/㎥. On the other hand, that of C15 exist at surface layer. In this area, DIN and DIP concentrations increased by input sources such as rainfall and benthic flux before the bloom of phytoplankton. Accumulation of phytoplankton occurred at subsurface layer by the rapid uptake of DIN, especially nitrate ion, when strong thermocline existed as approach to the afternoon, which led to the increase of organics in water column and oxygen deficiency water mass at bottom layer until late at evening. Since then, DIN increases gradually as water temperature decrease to minimum. The quantitative understanding of nitrogen of fluxed to and from the various sources is necessary for environmental management.

• Journal of the Korean Society for Marine Environment & Energy
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• v.3 no.4
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• pp.76-90
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• 2000

The estimation of material cycle of pollutants is necessary for the environment management in coastal zone. Model for material budgets are useful tools to understand the phenomena of natural system and to provide an insight into the complex processes including physical, chemical and biological processes occuring in natural system. Budgets of fresh water, salt and nutrients were estimated in order to clarify the characteristics of seasonal material cycle in Keum river estuary. Inflow volumes of freshwater into system was approximately 1.014×10/sup 8/～12.565×10/sup 8/m³/month and discharge in Keum river has occupied 99.7% of total freshwater. Seasonal variations of freshwater volume in the system were found to be very high in the range of about 4 ～ 14 times due to rainfall in summer season. Existing water mass of freshwater in system calculated by salt budget was approximately 0.339×10/sup 8/～0.652×10/sup 8/m³. Mean residence time of freshwater was calculated to be about 1.6～10.0day, and exchange time was calculated to be about 2.2～11.9day. Mean residence time was short as 1.6day in summer due to precipitation, and long as 10.1day in winter due to a drought. Inflow masses of DIP and DIN were approximately 5.57～32.68ton/month and 234.93～2,373.39ton/month, respectively. Seasonal inflow mass of DIP was larger than the outflow mass except for summer season. Thus, we postulate that accumulation of DIP in the system will happen. Residence times of DIP and DIN were calculated to be 1.1～6.4day and 1.8～10.9day, respectively. The ratio of water residence time versus DIP, DIN residence time was calculated to be 0.39～2.31 times and 0.83～1.13 times, respectively.

• IMMUNE NETWORK
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• v.3 no.3
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• pp.211-218
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• 2003

Background: Vitamin C is an essential nutrient, taken as a daily supplement by many people. Recently, high-dose vitamin C is considered as a therapeutic regimen in some clinical situations. Until now, few studies have been done with the effects of high-dose vitamin C on the immune response. Methods: In this experiment, the effects of high-dose vitamin C on cell-mediated immune response in immunologically competent mice were evaluated. After intraperitoneal injection of 2.5, 5, or 10 mg/day of vitamin C for 10 days, delayed type hypersensitivity (DTH) was provoked against DNFB in the pinnae as a model for cell-mediated immune response. Severity of DTH reaction was evaluated as the thickness of pinnae, and the vitamin C levels were measured in the serum, liver, kidney, lung, pinnae, and splenocytes. Results: After challenge, the thickness increased at its peak on the $2^{nd}$ day in all groups. On the first day, the pinnae were thicker in the injected groups than in the control. On the contrary, the increment of the pinnae thickness was attenuated and the number of cells infiltrated in the site of DTH decreased proportionately to the amount of vitamin C administered from the second day on. With vitamin C exogenously given, the serum level peaked at 30 min after injection, and returned abruptly to its basal level without accumulation. However, it accumulated in the liver, kidney, and especially in the pinnae inflamed and splenopcytes, proportionately to the amount administered. Conclusion: Based on these results, it is suggested that, in one hand, exogenously administered high-dose vitamin C accumulated in the splenocytes and presumably changed the function of them resulting in the augmented cell-mediated immune response, as was revealed in the first day of DTH reaction. On the other hand, it seems likely that the vitamin C also showed anti-inflammatory effects.

• Journal of Bio-Environment Control
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• v.4 no.1
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• pp.80-88
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• 1995

This survey has been conducted, mostly in inorganic ions, to get some basic data for the culture solution composition, analyzing water quality of some hydroponic farms. pH range was shown from 5.95 to 7.61 and the average of 6.75. Relatively wide range of EC, from 0.07 to 0.97 mS/cm and the average of 0.35 mS/cm were obtained. 19.5 percent of farms investigated showed over 0.5 mS/cm of EC, which means more careful culture solution composition and its management are needed in these farms. Na concentration ranged from 5.0 to 41.4 ppm and Cl concentration ranged from 10 to 99 ppm were shown and their average were 20.38 ppm and 35.16 ppm, respectively. Higher Na concentration compared to standard of 11.5 ppm was shown in 75% of farms and Higher Cl concentration compared to standard of 35.5 ppm was shown in 33.3% of farms. These concentration were considered rather high, which can cause salt accumulation in substrate mats. Ca and Mg concentrations were ranged from 1.60 to 131 ppm and 0.96 to 34.1 ppm, respectively. Average concentrations were 26.11 ppm in Ca and 8.10 ppm in Mg. In case of HCO$_3$, 24 to 295 ppm of concentration range and average of 63.13 ppm were obtained. Fe range was 0.01 to 0.87 ppm and its average was 0.14 ppm. This results showed that Fe elimination was necessary in well water.

• Journal of Bio-Environment Control
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• v.14 no.4
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• pp.245-253
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• 2005

This study was carried out to determine the optimum EC supply by accumulation amount of solar radiation in closed system. EC concentration of drainage was higher than that of supply. The higher EC concentration of supply was, the lower pH of drainage was. It was no difference in the quality of cut rose 'Nobles' (Rosa hybrida), stem length, stem diameter, leaf number, petal length, and petal diameter by EC treatment by season. The yield was higher about $6\%$ in treatment of EC $1.3dS{\cdot}m^{-1}$ in April and May, about $10\%$ in treatment of EC $10dS{\cdot}m^{-1}$ in June, July and August, and about $10\%$ in treatment of EC 1.0 or $1.3dS{\cdot}m^{-1}$ in September and October than the others. In general, the yield of the cut rose was higher in treatment of (B) EC 1.3 mS/cm in spring, EC $1.0dS{\cdot}m^{-1}$ in summer, EC $1.3dS{\cdot}m^{-1}$ in autumn, and EC$1.6dS{\cdot}m^{-1}$ in winter.

• Journal of Plant Biotechnology
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• v.42 no.3
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• pp.186-195
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• 2015

Anthranilate synthase (AS) is a key enzyme in the biosynthesis of tryptophan (Trp), which is the precursor of bioactive metabolites like indole-3-acetic acid and other indole alkaloids. Alpha anthranilate synthase 2 (OsASA2) plays a critical role in the feedback inhibition of tryptophan biosynthesis. In this study, two vectors with single (F124V) and double (S126F/L530D) point mutations of the OsASA2 gene for feedback-insensitive ${\alpha}$ subunit of rice anthranilate synthase were constructed and transformed into wildtype Dongjinbyeo by Agrobacterium-mediated transformation. Transgenic single and double mutant lines were selected as a single copy using TaqMan PCR utilized nos gene probe. To select intergenic lines, the flanking sequence of RB or LB was digested with a BfaI enzyme. Four intergenic lines were selected using a flanking sequence tagged (FST) analysis. Expression in rice (Oryza sativa L.) of the transgenes resulted in the accumulation of tryptophan (Trp), indole-3-acetonitrile (IAN), and indole-3-acetic acid (IAA) in leaves and tryptophan content as a free amino acid in seeds also increased up to 30 times relative to the wildtype. Two homozygous event lines, S-TG1 and D-TG1, were selected for characterization of agronomic traits and metabolite profiling of seeds. Differentially expressed genes (DEGs), related to ion transfer and nutrient supply, were upregulated and DEGs related to co-enzymes that work as functional genes were down regulated. These results suggest that two homozygous event lines may prove effective for the breeding of crops with an increased level of free tryptophan content.