• Title/Summary/Keyword: Nursery Soil

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Infection of Daughter Plants by Fusarium oxysporum f. sp. fragariae through Runner Propagation of Strawberry (딸기 영양번식을 통한 Fusarium oxysporum f. sp. fragariae의 자묘 감염)

  • Nam, Myeong-Hyeon;Kang, Yang-Jae;Lee, In-Ha;Kim, Hong-Gi;Chun, Chang-Hoo
    • Horticultural Science & Technology
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    • v.29 no.3
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    • pp.273-277
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    • 2011
  • Fusarium oxysporum f. sp. fragariae (Fof), the causal agent of crown and root rot in strawberry, is the most serious soilborne disease of nursery plants in Korea. The possibility of infection by Fof through runner propagation from infected mother plants of strawberry cv. 'Kumhyang' was assessed in stolons and daughter plants hanging from raised beds. The number of daughter plants from an infected mother plant in plastic house and photosynthetic photon flux (PPF) system, 280 ${\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ was 2.7 and 3.8 plants after 58 days, respectively. However, healthy mother plants produced 6.5 and 8.4 daughter plants, respectively. The pathogen was detected in the uppermost portion of the stolon after 58 days, but was not detected further down the stolon. After 90 days, it was detected in all portions of the stolon between mother and $1^{st}$ daughter plant and in 60% of all $1^{st}$ daughter plants. The pathogen was not detected in the corresponding portions of the non-infected controls. These results show that infected mother plants can transmit Fof to their daughter plants without passing through the soil and $1^{st}$ daughter was used as mother plant in PPF system for propagating healthy plants.

In vitro Culture Response to NaCl of Korean Ginseng (Panax ginseng C.A. Meyer) Tissues (기내배양을 통한 고려인삼(Panax ginseng C.A. Meyer)조직의 NaCl에 대한 반응)

  • Yoon Jae-Ho;Song Won-Seob;Lee Mee Sook;Shin Dong-il;Yang Deok Chun
    • Korean Journal of Plant Resources
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    • v.18 no.1
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    • pp.123-130
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    • 2005
  • High salt concentrations in the ginseng nursery soil environment of Korea is one of important reducing factors for the stable production of quality ginseng. These studies were accomplished for check the response on germination of ginseng seed, somatic embryogenesis of zygotic embryo, and biosynthesis of ginsenoside from ginseng hairy root against NaCl. Ratio of germination was at the $3\%\;and\;84.5\%$ on the basic media with 0.1M and free of NaCl repectedly, but $0\%$ at the upper of 0.2M NaCl. Somatic embryogenesis from zygotic embryo were the highest when immatured embryo was cultured on free of NaCl concentration, and which was intend to decrease at treatment of NaCl. However, in case of using the matured embryo, treatment of 0.05M NaCl resulted in better embryogenesis than NaCl free media. Red pigment was synthesized from ginseng hairy root cultured on the medium with various NaCl concentration(from 0.04 to 0.08M) and its pigment was analyzed as spectrum of anthocyane by spectrophoto- meter scanning. This cell line biosynthesized lots of crude saponin and total ginsenoside than other cell lines, also had 2 times of panaxadiol than panaxatriol.

Pseudomonas oleovorans Strain KBPF-004 Culture Supernatants Reduced Seed Transmission of Cucumber green mottle mosaic virus and Pepper mild mottle virus, and Remodeled Aggregation of 126 kDa and Subcellular Localization of Movement Protein of Pepper mild mottle virus

  • Kim, Nam-Gyu;Seo, Eun-Young;Han, Sang-Hyuk;Gong, Jun-Su;Park, Cheol-Nam;Park, Ho-Seop;Domier, Leslie L;Hammond, John;Lim, Hyoun-Sub
    • The Plant Pathology Journal
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    • v.33 no.4
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    • pp.393-401
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    • 2017
  • Efforts to control viral diseases in crop production include several types of physical or chemical treatments; antiviral extracts of a number of plants have also been examined to inhibit plant viral infection. However, treatments utilizing naturally selected microorganisms with activity against plant viruses are poorly documented. Here we report isolation of a soil inhabiting bacterium, Pseudomonas oleovorans strain KBPF-004 (developmental code KNF2016) which showed antiviral activity against mechanical transmission of tobamoviruses. Antiviral activity was also evaluated in seed transmission of two tobamoviruses, Pepper mild mottle virus (PMMoV) and Cucumber green mottle mosaic virus (CGMMV), by treatment of seed collected from infected pepper and watermelon, respectively. Pepper and watermelon seeds were treated with culture supernatant of P. oleovorans strain KBPF-004 or control strain ATCC 8062 before planting. Seeds germinated after treatment with water or ATCC 8062 yielded about 60% CGMMV or PMMoV positive plants, whereas < 20% of KBPF-004-treated seeds were virus-infected, a significantly reduced seed transmission rate. Furthermore, supernatant of P. oleovorans strain KBPF-004 remodeled aggregation of PMMoV 126 kDa protein and subcellular localization of movement protein in Nicotiana benthamiana, diminishing aggregation of the 126 kDa protein and essentially abolishing association of the movement protein with the microtubule network. In leaves agroinfiltrated with constructs expressing the coat protein (CP) of either PMMoV or CGMMV, less full-size CP was detected in the presence of supernatant of P. oleovorans strain KBPF-004. These changes may contribute to the antiviral effects of P. oleovorans strain KBPF-004.

Minimum Raising Duration of Infant Rice Seedling for Machine Transplanting (벼 기계이앙용 어린모 최소 육묘기간)

  • Kim, Je-Kyu;Lee, Moon-Hee;Oh, Yun-Jin
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.37 no.1
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    • pp.59-67
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    • 1992
  • The duration of raising seedling of infant rice seedling(IRS) in machine transplanting is 8 to 10 days. This experiment was conducted to investigate the minimum duration of IRS's raising seedling and its method by the combination of several treatments such as plant growth regulator, root-break-seat and temperature. The rice seeds of Hwaseongbyeo was soaked in the solution of metalaxyl for 24 hours at room temperature. Metalaxyl (25% wettable powder), a fungicide, was used in 1, 000 times dilution as a promting substance on the root-mat formation of IRS. The application of fungicide in the nursery soil for the controlling of damping-off and physiologyical seedling rot was omitted due to the metalaxyl seed-soaking treatment. Seeding rate was 220g per seed tray (30$\times$60$\times$3cm). To promote the root-mat formation of IRS, the sheets of polyethylene vinyl and absorptive paper were placed bottom the seed tray. The root-mat formation of IRS was promoted at higher temperature and longer duration of raising seedling. The metalaxyl-treated seeds markedly increased the root length and rooting activity of IRS as compared with the control, thus the root-mat formation was excellent. The absorptive paper with polyethylene vinyl as root-break-seat showed a better root-mat formation compared with control, polyethylene vinyl, alone. The minimum duration of raising seedling of IRS was 5 days after sowing based on the root-mat formation and seedling height under the condition of metalaxyl seed treatment, absorptive paper with polyethylene vinyl as a root-break-seat and the raising seedling temperature 30/2$0^{\circ}C$ (day /night).

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A Study on Transition of Rice Culture Practices During Chosun Dynasty Through Old References IX. Intergrated Discussion on Rice (주요(主要) 고농서(古農書)를 통(通)한 조선시대(朝鮮時代)의 도작기술(稻作技術) 전개(展開) 과정(過程) 연구(硏究) - IX. 도작기술(稻作技術)에 대(對)한 종합고찰(綜合考察))

  • Guh, J.O.;Lee, S.K.;Lee, E.W.;Lee, H.S.
    • Korean Journal of Weed Science
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    • v.12 no.1
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    • pp.70-79
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    • 1992
  • From the beginning of the chosun dynasty, an agriculture-first policy was imposed by being written farming books, for instance, Nongsajiksul, matched with real conditions of local agriculture, which provided the grounds of new, intensive farming technologies. This farming book was the collection of good fanning technologies that were experienced in rural farm areas at that time. According to Nongsajiksul, rice culture systems were divided into "Musarmi"(Water-Seeded rice), /"Kunsarmi"(dry-seeded rice), /transplanted rice and mountainous rice (upland rice) culture. The characteristics of these rice cultures with high technologies were based of scientific weeding methods, improved fertilization, and cultivation works using cattle power and manpower tools systematically. Reclamation of coastal swampy and barren land was possible in virtue of fire cultivation farming(火耕) and a weeding tool called "Yoonmok"(輪木). Also, there was an improved hoe to do weeding works as well as thinning and heaping-up of soil at seeding stages of rice. Direct-seeded rice culture in flat paddy fields were expanded by constructing the irrigation reservoirs and ponds, and the valley paddy fields was reclaimed by constructing "Boh(洑)". These were possible due to weed control by irrigation waters, keeping soil fertility by inorganic fertilization during irrigation, and increased productivity of rice fields by supplying good physiological conditions for rice. Also, labor-saving culture of rice was feasible by transplanting but in national-wide, rice should not basically be transplanted because of the restriction of water use. Thus, direct-seeded rice in dry soils was established, in which rice was direct-seeded and grown in dry soils by seedling stages and was grown in flooded fields when rained, as in the book "Nongsajiksul". During the middle of the dynasty(AD 1495-1725), the excellent labor-saving farmings include check-rowing transplanting because of weeding efficiency and availability in rice("Hanjongrok"), and, nurserybed techniques (early transplanting of rice) were emphasized on the basis of rice transplanting ["Nongajibsung"]. The techniques for deep plowing with cattle powers and for putting more fertilizers were to improve the productivity of labor and land, The matters advanced in "Sanlimkyungje" more than in "Nongajibsung" were, development of "drybed of rice nursery stock", like "upland rice nursery" today, transplanting, establishment of "winter barly on drained paddy field, and improvement of labor and land-productivity in rice". This resulted in the community of large-scale farming by changing the pattern of small-farming into the production system of rice management. Woo-hayoung(1741-1812) in his book "Chonilrok" tried to reform from large-scale farmings into intensive farmings, of which as eminent view was to divide the land use into transplanting (paddy) and groove-seeding methods(dry field). Especially as insisted by Seo-yugo ("Sanlimkyungjeji"), the advantages of transplanting were curtailment of weeding labors, good growth of rice because of soil fertility of both nurserybed and paddy field, and newly active growth because rice plants were pulled out and replanted. Of course, there were reestimation of transplanting, limitation of two croppings a year, restriction of "paddy-upland alternation", and a ban for large-scale farming. At that period, Lee-jiyum had written on rice farming technologies in dry upland with consider of the land, water physiology of rice, and convenience for weeding, and it was a creative cropping system to secure the farm income most safely. As a integrated considerations, the followings must be introduced to practice the improved farming methods ; namely, improvement of farming tools, putting more fertilizers, introduction of cultural technologies more rational and efficient, management of labor power, improvement of cropping system to enhance use of irrigation water and land, introduction of new crops and new varieties.

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Influence of Root Restriction Materials and Media on Soil Environment and Growth of Runner Plantlets during Propagation of 'Seolhyang' Strawberry (차근육묘를 위한 자재 및 배지 종류가 토양환경과 '설향' 딸기 자묘의 생장에 미치는 영향)

  • Park, Gab Soon;Chae, Soo Cheon;Oh, Chan Sik;Choi, Jong Myung
    • Horticultural Science & Technology
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    • v.33 no.4
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    • pp.511-517
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    • 2015
  • This research was conducted to evaluate the influence of root restriction materials and media on the growth of runner plantlets of 'Seolhyang' strawberry in a nursery field. To achieve this, the influence of three kinds of root media on the growth of runner plantlets was monitored when polyethylene film was used as the root restriction material. In addition, the influence of various root restriction materials (RRS) such as transparent polyethylene film (PE), non-woven fabric (NF), perforated polyethylene film (PP), and root proofing sheet (RPS) on the changes in volumetric water content (VWC) and temperature of root media as well as growth of runner plantlet were investigated when expanded rice hull (ERH) was used as the root medium. In the comparison of root media, growth parameters such as leaf area and crown thickness at 20 d after fixation as well as crown thickness and fresh weights of root and above-ground tissue at 40 d after runner plantlet fixation were higher in the ERH treatment than in sandy loam and loamy sand. When the influence of RRS was compared, the VWC of ERH was 55% just after irrigation, but decreased to 26% at just before irrigation. Ranges of the VWC as influenced by irrigation cycle were 16 to 10% in the PP and less than 10% in the NF and RPS. The soil temperature in the PE treatment was around $1^{\circ}C$ lower than in NF, PP, and RPS. The differences between day and night temperatures were also smaller in the PE treatment rather than those in NF, PP, and RPS. The growths of runner plantlet 50 d after fixation showed that plant heights as well as fresh weights of root and above-ground tissue were higher in the PE treatment than in NF, PP, and RPS. NF and PP did not effectively restrict roots inside the medium and the roots of runner plantlets penetrated through the root restriction materials resulting in the formation of root system below the restriction materials. The above results indicate that ERH is more effective than sandy loam or loamy sand as root medium. PE rather than NF, PP, or RPS as root restriction material resulted in better growth of runner plantlets in propagation of 'Seolhyang' strawberry. The results of this research will be used for production of high quality runner plantlets in strawberry propagation.

Assessment of Substantial Equivalence and Environmental Risk for Event Selection of Genetically Modified Chrysanthemum (국화의 GM 이벤트 선발을 위한 실질적 동등성 및 환경위해성 평가)

  • Lim, Jin Hee;Shim, Myung Syun;Oh, Kwan Hun;Seo, Ji Yeon;Lee, Su Young;Park, Sang Kun;Shin, Hak Ki;Cho, Myoung Rae
    • Horticultural Science & Technology
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    • v.32 no.4
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    • pp.517-524
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    • 2014
  • As an assessment of substantial equivalence and environmental risks of branchless chrysanthemum GM lines (LeLS69, LeLS76, LeLS80) developed in Korea, we investigated their degree of branchlessness and developmental characteristics including growth and flowering, as well as the stability of populations of insect pests and soil microbes. The GM lines were compared with a control variety, 'Jinba'. LeLS80 showed the highest level of branchlessness among the three GM lines, whereas abnormal axillary buds developed in lines LeLS76 and LeLS69. There were no significant differences between the growth characteristics of 'Jinba' and the GM lines. The branchlessness LeLS80 was increased during the summer season, suggesting that it is a useful germplasm for developing elite branchless cultivars. These GM lines showed no significant differences in the occurrence of five insect pests including aphids, mites, leaf miners, thrips, and whiteflies. Similarly, for soil microbes including bacteria and actinomycetes, there were no significant differences between 'Jinba' and the GM lines. However, there was a higher occurrence of fungi in the GM lines compared to 'Jinba'. Our findings suggest that 'Jinba' and the GM lines are substantially equivalent, although further monitoring studies are needed to confirm this.

Quality of Yellow Poplar (Liriodendron tulipifera) Seedlings by the Method of Seedling Production (백합나무 양묘방법에 따른 묘목품질 비교)

  • Ryu, Keun-Ok;Song, Jeong-Ho;Choi, Hyung-Soon;Kwon, Hae-Yun;Kwon, Yong-Rak
    • Journal of Korean Society of Forest Science
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    • v.96 no.3
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    • pp.307-316
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    • 2007
  • Yellow poplar (Liriodendron tulipifera L.) has low germination rate relatively other species, so the seedling production of Yellow poplar is a hard task. Accordingly this study was conducted to determine the optimal germination conditions for healthy seedling production and to promote survival rate after afforestation. Gemination percentage was examined at different media and seed covering materials using planting flats in the greenhouse. The best germination percentage was observed in sand for media and compound soil for covering materials. But it was time to transplant, seedlings became a poor character (i.e. height, root length, number of root, dry weight) in sand for media. In order to produce healthy seedlings, each different medium was compounded with TKS-2 (this is a gardening bed soil.) in the ratio 1:1 (v/v.), and compared two conditions. Quality of seedling was better than not mixed TKS-2 into each medium. Transplanting seedlings from greenhouse to nursery grew up rapidly 2 months later (early in August~early in October). Growth amount during two months corresponded to 85.6% and 71.3% in total growth amount of height and diameter at root collar, respectively. In the case of the competition-density effect on yellow-poplar seedlings, direct seedling produced the maximum 35 standard seedlings above 8 mm of root collar diameter per $m^2$, while transplanting seedling produced the maximum 64 standard seedlings per $m^2$. And produced seedlings of two way were significantly different rootlet while axial root and lateral root was not significantly different.

Growth Characteristics and Economic Efficiency of Nursery Plants Production According to Transplanting Container for Acclimatization of Mass Propagated Plantlets via Bioreactor Culture of Ever-bearing Strawberry 'Goha' (생물반응기를 통해 대량증식된 사계성 딸기 '고하' 소식물체의 순화용기에 따른 생육특성과 묘생산 경제성)

  • Lee, Jong-Nam;Kim, Hye-Jin;Kim, Ki-Deog;Im, Ju-Sung;Lim, Hak-Tae;Yeoung, Young-Rok
    • Horticultural Science & Technology
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    • v.30 no.4
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    • pp.437-441
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    • 2012
  • This study was conducted to find out the optimum container for increasing acclimatization rate of in vitro mass propagated plantlets of Ever-bearing strawberry (Fragaria ${\times}$ ananassa Duch.) via bioreactor. Four types of containers were used such as transparent plastic container (TPC), plug tray (PT), I-pot (IP), and black vinyl pot (BVP). Number of date maintaining soil water content above 10% was five days in TPC, three to four days in BVP, two days in PT, and one day in IP. Survival rate of plantlets was 80% in BVP, 70% in TPC, 55% in IP, and 15% in PT. In TPC, growth increment of plantlets was the greatest among all the tested containers and the lowest in IP. Numbers of runner per plant were 3.3 in BVP, 2.9 in TPC, 1.6 in PT, and 1.2 in IP. Total cost was 44,405,300 won/10 a in BVP, resulting in reducing more 6,659,400 won/10 a than IP's (51,064,700 won/10 a). Around 102,718 plants/10 a were produced by using BVP, suggesting that 30,265.1 plants/10 a more could be produced than IP (72,452.9 plants/10 a). Production cost per plant was 432.3 won in BVP, resulting in reducing 272.5 won than IP's (704.8 won). As a result, BVP was appropriate for acclimatization of in vitro plantlets through bioreactor system.

Effects of Arbuscular Mycorrhizal Fungus, Glomus intraradices, on the Growth, Photosynthesis and Phosphorus Content of Robinia pseudoacacia Seedlings Treated with Simulated Acid Rain (Glomus 내생균근균 접종이 인공산성우를 처리한 아까시나무 묘목의 생장, 광합성, 인 함량에 미치는 영향)

  • Kim, Eun Ho;Lee, Kyung Joon;Lee, Kyu Hwa
    • Journal of Korean Society of Forest Science
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    • v.95 no.6
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    • pp.735-742
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    • 2006
  • The objective of this study was to elucidate the tolerance of woody plants to simulated acid rain in relation to mycorrhizal inoculation. Germinating seedlings of Robinia pseudoacacia were planted in 1I pots with autoclaved soil mixture of vermiculite, sand and nursery soil at 1:1:1 ratio. Each pot was inoculated with both crushed root nodules from a wild tree of the same species and commercial arbuscular mycorrhizal inoculum of Glomus intraradices at the time of planting the seedlings. Simulated acid rains at pH 2.6, 3.6, 4.6, and 5.6 were made by mixing sulfuric acid and nitric acid at 3: 1 ratio. Each pot received nutrient solution without N and P, and was also supplied with 180 ml of the one pH level of the acid rains once a week for 50 days. The plants were grown in the green house. At the end of experimental period, plants were harvested to determine contents of chlorophyll, mineral nutrients and net photosynthesis in the tissues, dry weight of the plants, and mycorrhizal infection in the roots. Mycorrhizal infection rate was significantly reduced only at pH 2.6, which meant vitality of G intraradices was inhibited at extremely low pH. Height growth, dry weight production, nodule production and chlorophyll content were increased by mycorrhizal infection in all the pH levels except pH 3.6. Particularly, mycorrhizal inoculation increased root nodule production by 85% in pH 5.6 and 45% in 4.6 treatments. But the stimulatory effect of mycorrhizal inoculation on nodule production was reduced at pH 3.6 and 2.6. Net photosynthesis was increased by mycorrhizal infection in all the pH levels. The phosphorus(P) content in the tissues was increased by 43% in average by mycorrhizal inoculation, which was statistically significant except in pH 2.6. It was concluded that mycorrhizal inoculation of Robinia pseudoacacia would enhance growth and resistance of the plants to acid rain by improving the photosynthesis, phosphorus nutrition, and more nodule production.