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Growth Characteristics and Economic Efficiency of Nursery Plants Production According to Transplanting Container for Acclimatization of Mass Propagated Plantlets via Bioreactor Culture of Ever-bearing Strawberry 'Goha'

생물반응기를 통해 대량증식된 사계성 딸기 '고하' 소식물체의 순화용기에 따른 생육특성과 묘생산 경제성

  • Lee, Jong-Nam (Highland Agriculture Research Center, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Hye-Jin (Highland Agriculture Research Center, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Ki-Deog (Highland Agriculture Research Center, National Institute of Crop Science, Rural Development Administration) ;
  • Im, Ju-Sung (Highland Agriculture Research Center, National Institute of Crop Science, Rural Development Administration) ;
  • Lim, Hak-Tae (Department of Bio-Health Technology, Kangwon National University) ;
  • Yeoung, Young-Rok (Department of Plant Science, Gangneung-Wonju National University)
  • 이종남 (국립식량과학원 고령지농업연구센터) ;
  • 김혜진 (국립식량과학원 고령지농업연구센터) ;
  • 김기덕 (국립식량과학원 고령지농업연구센터) ;
  • 임주성 (국립식량과학원 고령지농업연구센터) ;
  • 임학태 (강원대학교 생명건강공학전공) ;
  • 용영록 (강릉원주대학교 식물생명과학과)
  • Received : 2012.03.14
  • Accepted : 2012.05.22
  • Published : 2012.08.30

Abstract

This study was conducted to find out the optimum container for increasing acclimatization rate of in vitro mass propagated plantlets of Ever-bearing strawberry (Fragaria ${\times}$ ananassa Duch.) via bioreactor. Four types of containers were used such as transparent plastic container (TPC), plug tray (PT), I-pot (IP), and black vinyl pot (BVP). Number of date maintaining soil water content above 10% was five days in TPC, three to four days in BVP, two days in PT, and one day in IP. Survival rate of plantlets was 80% in BVP, 70% in TPC, 55% in IP, and 15% in PT. In TPC, growth increment of plantlets was the greatest among all the tested containers and the lowest in IP. Numbers of runner per plant were 3.3 in BVP, 2.9 in TPC, 1.6 in PT, and 1.2 in IP. Total cost was 44,405,300 won/10 a in BVP, resulting in reducing more 6,659,400 won/10 a than IP's (51,064,700 won/10 a). Around 102,718 plants/10 a were produced by using BVP, suggesting that 30,265.1 plants/10 a more could be produced than IP (72,452.9 plants/10 a). Production cost per plant was 432.3 won in BVP, resulting in reducing 272.5 won than IP's (704.8 won). As a result, BVP was appropriate for acclimatization of in vitro plantlets through bioreactor system.

본 실험은 생물반응기를 통해 대량증식된 사계성 딸기 조직배양묘의 적정 순화용기를 선발하기 위해 실시하였다. 순화용기는 플라스틱투명용기, 플러그 트레이, I-포트 및 흑색비닐포트 등 4가지를 사용하였다. 토양함수율 10%이상 지속일수는 플라스틱 투명용기가 5일, 흑색비닐포트가 3-4일, 플러그 트레이 2일, I-포트가 1일 순이었다. 생존율은 흑색비닐포트가 80%, 플라스틱투명용기가 70%, I-포트가 55% 및 플러그 트레이가 15% 순으로 나타났다. 또한 생육량은 플라스틱 투명용기가 가장 왕성하였고, 흑색비닐포트와 플러그 트레이는 비슷하였으며, I-포트가 가장 저조하였다. 런너 발생은 흑색비닐포트가 주당 3.3개로 가장 많았으며, 플라스틱투명용기가 2.9개, 플러그 트레이가 1.6개 및 I-포트가 1.2개 순으로 나타났다. 총 생산비용은 흑색비닐포트가 44,405,300원/10a로 I-포트의 51,064,700원/10a보다 6,659,400원/10a이 더 절감되었으며, 총 생산주수도 흑색비닐포트가 102,718주/10a로 I-포트의 72,452.9주/10a보다 30,265.1주/10a 더 생산되었다. 또한 주당 생산비용은 흑색비닐포트가 432.3원으로 I-포트의 704.8원에 비해 272.5원의 감소하였다. 따라서 생물반응기를 통해 대량증식된 사계성 딸기 조직배양묘의 순화용기는 흑색비닐포트가 적당하였다.

Keywords

References

  1. Carmi, A. 1995. Growth, water transport and transpiration in root -restricted plants of bean, and their relation to abscisic acid accumulation. Plant Sci. 107:69-76. https://doi.org/10.1016/0168-9452(95)04097-E
  2. Grout, B.W.W. and M.J. Aston. 1977. Transplanting cauliflower plants regenerated from meristem culture. I. Water loss and water transfer related to changes in leaf wax to xylem regeneration. Hort. Res. 17:1-7.
  3. Hess, L. and H. De Kroon. 2007. Effects of rooting volume and nutrient availability as an alternative explanation for root self/ non-self discrimination. J. Ecol. 95:241-251. https://doi.org/10.1111/j.1365-2745.2006.01204.x
  4. Kim, H.J., J.N. Lee, K.D. Kim, J.S. Im, H.T. Lim, and Y.R. Yeoung. 2011. Suitable hormone-free medium for in vitro mass propagation via bioreactor culture of Ever-bearing strawberry. J. Plant Biotechnol. 38:221-227. https://doi.org/10.5010/JPB.2011.38.3.221
  5. Lee, J.N., H.Y. Kim, K.D. Kim, Y.S. Kwon, H.T. Lim, and Y.R. Yeoung. 2010a. In vitro mass propagation and economic effects of bioreactor culture in Ever-bearing strawberry 'Goha'. Kor. J. Hort. Sci. Technol. 28:845-849.
  6. Lee, J.N., H.Y. Kim, K.D. Kim, Y.S. Kwon, J.S. Im, Y.R. Yeoung, and H.T. Lim. 2010b. Appropriate in vitro culture conditions of growing medium for new ever-bearing strawberry 'Goha'. Kor. J. Hort. Sci. Technol. 28:1051-1056.
  7. Ray, J.D. and T.R. Sinclair. 1998. The effect of pot size on growth and transpiration of maize and soybean during water deficit stress. J. Exp. Bot. 49:1381-1386. https://doi.org/10.1093/jxb/49.325.1381
  8. Rita, I. and E.I.S. Floh. 1995. Tissue culture and micropropagation of Cuphea ericoides, a potential source of medium-chain fatty acids. Plant Cell, Tiss. Org. Cult. 40:187-195. https://doi.org/10.1007/BF00037674
  9. Ronchi, C.P., F.M. DaMatta, K.D. Batista, G. Moraes, M.E. Loureiro, and C. Ducatti. 2006. Growth and photosynthetic down-regulation in Coffee arabica in response to restricted root volume. Functional Plant Biol. 33:1013-1023. https://doi.org/10.1071/FP06147
  10. Skirvin, R.M. and S. Sriskandarajah. 1993. The use of fogging and phototron units to acclimatize in vitro-derived apple shoots. HortTechnology 3:208-210.
  11. Slavtcheva, T. and V. Dimitrova. 1997. Gas exchange with in vitro cultured vine plants during their acclimatization. Riv. Vitic. Enol. 2:3-9.
  12. Sutter, E. and R.W. Langhans. 1982. Formation of epicuticular wax and its effect on water loss in cabbage plants regenerated from shoot-tip culture. Can. J. Bot. 60:2896-2902. https://doi.org/10.1139/b82-350
  13. Thomas, P. 1998. Humid incubation period and plantlet age influence acclimatization and establishment of micropropagated grapes. In Vitro Cell. Dev. Biol. Plant. 34:52-56. https://doi.org/10.1007/BF02823123
  14. Wetzestein, H.Y. and H.E. Sommer. 1982. Leaf anatomy of tissue -cultured Liquidambar styraciflua (Hammelidaceae) during acclimatization. Amer. J. Bot. 69:1579-1586. https://doi.org/10.2307/2442913
  15. Zhu, L.N., S.P. Wang, T.Y. Yang, C.X. Zhang, and W.P. Xu. 2006. Vine growth and nitrogen metabolism of 'Fujiminori' grapevine in response to root restriction. Sci. Hort. 107:143-149. https://doi.org/10.1016/j.scienta.2005.06.007

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