Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
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Growth Characteristics of in Vitro Mass Propagated Plantlets of Ever-bearing Strawberry 'Goha' according to Aeration Rate in Bioreactor

사계성 딸기 '고하' 조직배양묘의 대량증식 시 생물반응기 내 공기주입량에 따른 생육 특성

  • Kim, Hye-Jin (Highland Agriculture Research Center, National Institute of Crop Science) ;
  • Lee, Jong-Nam (Highland Agriculture Research Center, National Institute of Crop Science) ;
  • Kim, Ki-Deog (Highland Agriculture Research Center, National Institute of Crop Science) ;
  • Im, Ju-Sung (Highland Agriculture Research Center, National Institute of Crop Science) ;
  • Lim, Hak-Tae (Department of Bio-Health Technology, Kangwon National University) ;
  • Yeoung, Young-Rok (Department of Plant Science, Gangneung-Wonju National University)
  • 김혜진 (국립식량과학원 고령지농업연구센터) ;
  • 이종남 (국립식량과학원 고령지농업연구센터) ;
  • 김기덕 (국립식량과학원 고령지농업연구센터) ;
  • 임주성 (국립식량과학원 고령지농업연구센터) ;
  • 임학태 (강원대학교 생명건강공학전공) ;
  • 용영록 (강릉원주대학교 식물생명과학과)
  • Received : 2012.03.14
  • Accepted : 2012.05.11
  • Published : 2012.08.30
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Abstract

This study was conducted to determine the optimal aeration rate for mass propagation of ever-bearing strawberry by bioreactor culture. The aeration rate was treated in four levels: 0.1 vvm (air volume/medium volume/min), 0.2 vvm, 0.3 vvm, and 0.4 vvm. In 0.2 vvm conditions, shoot length was the longest at 9.03 cm in bioreactor culture, leave numbers were 40.4 ea and fresh weight was 6,106 mg. Plant growth rate at 0.2 vvm condition was faster than other treatments. In the aeration condition, 0.2 vvm was most effective to increase aerial part growth and to decrease medium consumption. As the culture periods increased, the fresh weight also increased rapidly. After six weeks of cultivation, shoots were emerged with 10.4 ea per plantlet, resulting in developing a complete plant. As a result, the bioreactor culture system for mass propagation of strawberry is required to continuously supply the air by 0.2 vvm speed and cultivate at least for six weeks.

본 실험은 사계성 딸기의 무병묘 생산을 위한 생물반응기 배양 시 공기주입량에 따른 생육특성을 비교하고, 적정 공기주입량을 구명하기 위해 실시하였다. 생물반응기 내 공기주입량은 0.1vvm, 0.2vvm, 0.3vvm 및 0.4vvm 등 4 수준으로 처리하였다. 공기주입량 0.2vvm에서 초장이 9.03cm로 가장 길었으며, 엽수는 40.4개, 생체중은 6,106mg으로 타처리구에 비해 생육이 왕성하였다. 생물반응기 배양기간이 길어짐에 따라 잎, 뿌리 등이 점진적으로 많이 발생하였으며, 유식물체의 생체중도 급격히 늘어났으며, 배양 6주째에는 신초수가 주당 10.4개 발생하였으며 완전한 하나의 독립된 식물체로 발달되었다. 그리고 생물반응기 내의 공기는 0.2vvm으로 지속적으로 공급해주며, 최소 6주간 배양해야 완전한 식물체로 성장하였다.

Keywords

References

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