Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
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Development of Efficient Screening Methods for Resistance of Tomato to Fusarium oxysporum f. sp. lycopersici

토마토 시들음병에 대한 효율적인 저항성 검정법 확립

  • Park, Myung-Soo (Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology) ;
  • Jeong, Bo-Ram (Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology) ;
  • Jang, Kyoung-Soo (Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology) ;
  • Choi, Yong-Ho (Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology) ;
  • Kim, Jin-Cheol (Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology) ;
  • Choi, Gyung-Ja (Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology)
  • 박명수 (한국화학연구원 바이오화학연구센터) ;
  • 정보람 (한국화학연구원 바이오화학연구센터) ;
  • 장경수 (한국화학연구원 바이오화학연구센터) ;
  • 최용호 (한국화학연구원 바이오화학연구센터) ;
  • 김진철 (한국화학연구원 바이오화학연구센터) ;
  • 최경자 (한국화학연구원 바이오화학연구센터)
  • Received : 2012.04.17
  • Accepted : 2012.06.11
  • Published : 2012.08.30
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Abstract

This study was conducted to establish an efficient screening method for resistant tomato to Fusarium wilt caused by Fusarium oxysporum f. sp. lycopersici (FOL). The resistance degrees of the six commercial cultivars of tomato to the pathogen were evaluated by dipping roots of the seedlings in spore suspension of five FOL isolates. On the basis of the results, two cultivars (Dotaerangmaster, resistant cultivar to FOL race 1; Supersunload, resistant cultivar to FOL race 2) and two isolates (KACC40043, FOL race 2; TF104, FOL race 3) were selected for system establishment. The disease development of the FOL isolates on the cultivars according to several conditions including root wounding, incubation temperature, inoculum concentration and dipping period of roots in spore suspension was investigated. The resistance of each cultivar to the disease was a race-specific response and hardly affected by the tested conditions except for incubation temperature of $20^{\circ}C$. The optimum temperature for disease development caused by FOL was 25 to $30^{\circ}C$. On the basis of the results, we suggest that an efficient screening method for resistant tomato cultivars to Fusarium wilt is to dip the non-cut roots of tomato seedlings at two-leaf stage in spore suspension of $1{\times}10^7\;conidia{\cdot}mL^{-1}$ for 0.5 hours and transplant the seedling to plastic pot with horticulture nursery media, and then to cultivate the plants in a growth room at $25^{\circ}C$ for 3 weeks with 12 hours light a day.

Fusarium oxysporum f. sp. lycopersici(FOL)에 의해 발생하는 토마토 시들음병에 대한 효과적인 저항성 검정법을 확립하기 위하여, 시판 중인 토마토 6개 품종에서 FOL 5개 균주에 의한 시들음병 발생을 조사한 후에 토마토 2개 품종(도태랑마스터, race 1 저항성; 슈퍼선로드, race 1과 2 저항성)과 FOL 2개 균주(KACC40043, race 2; TF104, race 3)를 선발하였다. 선발한 토마토 품종과 FOL 균주를 이용하여 뿌리 상처 유무, 재배 온도, 접종원 농도 및 포자현탁액에 침지하는 시간 등에 따른 시들음병 발생을 조사하였다. FOL에 대한 토마토 품종의 저항성 및 감수성 반응은 레이스 특이적이며, $20^{\circ}C$의 재배온도를 제외한 실험한 모든 발병 조건에 의해 이들 반응은 영향을 받지 않았다. 그리고 FOL에 의한 토마토 시들음병 발생의 최적 온도는 $25-30^{\circ}C$임을 알 수 있었다. 따라서, 토마토 시들음병에 대한 저항성을 효과적으로 검정하는 방법으로 2엽기 토마토 유묘의 뿌리를 $1{\times}10^7conidia/mL$ 농도의 FOL 포자 현탁액에 30분간 침지한 후에 원예용 상토에 이식하고 $25^{\circ}C$ 생육상에서 하루에 12시간씩 광을 조사하면서 3주 동안 재배하는 것을 제안한다.

Keywords

References

  1. Alexander, L.J. and M.M. Hoover. 1955. Disease resistance in wild species of tomato. Ohio Agric. Exp. Stn. Res. Bull. 752:1-76.
  2. Alexander, L.J. and C.M. Tucker. 1945. Physiologic specialization in the tomato wilt fungus Fusarium oxysporum f. sp. lycopersici. J. Agric. Res. 70:303-313.
  3. Bost, S.C. 2001. First report of Fusarium oxysporum f. sp. lycopersici race 3 on tomato in Tennessee. Plant Dis. 85:802.
  4. Bohn, G.W. and C.M. Tucker. 1939. Immunity to Fusarium wilt in the tomato. Science 89:603-604. https://doi.org/10.1126/science.89.2322.603
  5. Bournival, B.L., J.W. Scott, and C.E. Vallejos. 1989. An isozyme marker for resistance to race 3 of Fusarium oxysporum f. sp. lycopersici in tomato. Theor. Appl. Genet. 78:489-494. https://doi.org/10.1007/BF00290832
  6. Bournival, B.L. and C.E. Vallejos. 1991. New sources of genetic resistance to race 3 of Fusarium wilt of tomato. Plant Dis. 75:281-284. https://doi.org/10.1094/PD-75-0281
  7. Cai, G., L.R. Gale, R.W. Schneider, H.C. Kistler, R.M. Davis, K.S. Elias, and E.M. Miyao. 2003. Origin of race 3 of Fusarium oxysporum f. sp. lycopersici at a single site in California. Phytopathology 93:1014-1022. https://doi.org/10.1094/PHYTO.2003.93.8.1014
  8. Cirulli, M. and L.J. Alexander. 1966. A comparison of pathogenic isolates of Fusarium oxysporum f. sp. lycopersici and different sources of resistance in tomato. Phytopathology 56:1301-1304.
  9. Davis, R.M., K.A. Kimble, and J.J. Farrar. 1988. A third race of Fusarium oxysporum f. sp. lycopersici identified in California. Plant Dis. 72:453.
  10. El Mohtar, C.A., H.S. Atamian, R.B. Dagher, Y. Abou-Jawdah, M.S. Salus, and D.P. Maxwell. 2007. Marker-assisted selection of tomato genotypes with the I-2 gene for resistance to Fusarium oxysporum f. sp. lycopersici race 2. Plant Dis. 91:758-762. https://doi.org/10.1094/PDIS-91-6-0758
  11. Gabe, H.L. 1975. Standardization of nomenclature for pathogenic races of Fusarium oxysporum f. sp. lycopersici. Trans. Br. Mycol. Soc. 64:156-159. https://doi.org/10.1016/S0007-1536(75)80089-1
  12. Grattidge, R. and R.G. O'Brien. 1982. Occurrence of a third race of Fusarium wilt of tomatoes in Queensland. Plant Dis. 66:165-166. https://doi.org/10.1094/PD-66-165
  13. Huang, C.-C. and P. Lindhout. 1997. Screening for resistance in wild Lycopersicon species to Fusarium oxysporum f. sp. lycopersici race 1 and race 2. Euphytica 93:145-153. https://doi.org/10.1023/A:1002943805229
  14. Jones, J.B., J.P. Jones, R.E. Stall, and T.A. Zitter. 1991. Compendium of tomato diseases. American Phytopathological Society, St. Paul, MN.
  15. Kim, J.T. 2005. Pathogenicity and ecophysiological characteristics of Fusarium oxysporum causing tomato wilt disease. PhD Diss., Chungnam National Univ., Daejeon, Korea.
  16. Laterrot, H. and J. Philouze. 1984. Recombination between resistance to pathotype 1 (I-2 allele) and susceptibility to pathotype 0 (I+ allele) of Fusarium oxysporum f. sp. lycopersici in tomato (Lycopersicon esculentum Mill.). Proc. Mtg. Eucarpia Tomato Working Group 9:70-74.
  17. Marlatt, M.L., J.C. Correll, P. Kaufmann, and P.E. Cooper. 1996. Two genetically distinct populations of Fusarium oxysporum f. sp. lycopersici race 3 in the United States. Plant Dis. 80:1336-1342. https://doi.org/10.1094/PD-80-1336
  18. McGrath, D.J., D. Gillespie, and L. Vawdrey. 1987. Inheritance of resistance to Fusarium oxysporum f. sp. lycopersici races 2 and 3 in Lycopersicon pennellii. Aust. J. Agric. Res. 38:729-733. https://doi.org/10.1071/AR9870729
  19. Mes, J.J., E.A. Weststeijn, F. Herlaar, J.J.M. Lambalk, J. Wijbrandi, M.A. Haring, and B.J.C. Cornelissen. 1999. Biological and molecular characterization of Fusarium oxysporum f. sp. lycopersici divides race 1 isolates into separate virulence groups. Phytopathology 89:156-160. https://doi.org/10.1094/PHYTO.1999.89.2.156
  20. Rural Development Administration (RDA). 2009. Tomato. RDA, Suwon, Korea.
  21. Scott, J.W. and J.P. Jones. 1989. Monogenic resistance in tomato to Fusarium oxysporum f. sp. lycopersici race 3. Euphytica 40:49-53.
  22. Stall, R.E. and J.M. Walter. 1965. Selection and inheritance of resistance in tomato to isolates of race 1 and 2 of the Fusarium wilt organism. Phytopathology 55:1213-1215.
  23. Volin, R.B. and J.P. Jones. 1982. A new race of Fusarium wilt of tomato in Florida and sources of resistance. Proc. Fla. State Hortic. Soc. 95:268-270.
  24. Walker, J.C. 1971. Fusarium wilt of tomato. Monogr. 6. American Phytopathological Society, St. Paul, MN.

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  1. 토마토 시들음병에 대한 간편 대량 저항성 검정법 vol.31, pp.1, 2012, https://doi.org/10.7235/hort.2013.12134