• The Journal of the Korea Contents Association
• /
• v.16 no.10
• /
• pp.435-442
• /
• 2016

Obesity extends the global economic burden and it causes that the failure of a reduction of physical activity, and diet management. In this work, nutritional information and personalized diet based on calorie supply system and is discriminatory utilized the obesity-related SNP information in order to recommend a personalized functional foods. This study performed a GWAS analysis for the excavation of a Korean-specific and obesity-related SNP, which utilizes genetic information were recommended by entering a personalized diet in accordance with the SNP genotype-phenotype information. In addition, we integrated Database with relation of nutrient for utilizing the USDA Food information and it was applied to recommend with Sickdanome. As a result, the obesity-related SNP information was confirmed in the sample which has the normal value BMI. In this study, we have recognized that the phenotype information related obesity, BMI is inconsistent with the SNP genotype information. This result is shown that it is necessary to provide the personalized dietary SickdanOme recommendation based on the both pheotype-genotype information.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
• /
• v.13 no.1
• /
• pp.1-10
• /
• 2015

In the safety assessment of the geological disposal system of the radioactive wastes, the abnormal scenarios, in which the system is impacted by the abnormal events, need to be considered in addition to the reference scenario. In this study, characterization and prediction of well intrusion as one of the abnormal events which will impact the disposal system were conducted probabilistically and statistically for the safety assessment. The domestic well development data were analyzed, and the prediction methodologies of the well intrusion were suggested with a computation example. From the results, the annual well development rate per unit area in Korea was about 0.8 well/yr/km² in the conservative point of view. Considering the area of the overall disposal system which is about 1.5 km², the annual well development rate within the disposal system could be 1.2 well/yr. That is, it could be expected that more than one well would be installed within the disposal system every year after the institutional management period. From the statistical analysis, the probabilistic distribution of the well depth followed the log-normal distribution with 3.0363 m of mean value and 1.1467 m of standard deviation. This study will be followed by the study about the impacts of the well intrusion on the geological disposal system, and the both studies will contribute to the increased reliability of safety assessment.

• Reproductive and Developmental Biology
• /
• v.37 no.1
• /
• pp.35-40
• /
• 2013

The objective of this study was to investigate the effects of abnormal ovarian cycles after superovulation treatment of Holstein Donor Cows. CIDRs were inserted into the vaginas of twenty two head of Holstein cows, regardless of estrous cycle. Superovulation was induced using folliclar stimulating hormone (FSH). For artificial insemination, donor cows were injected with $PGF_2{\alpha}$ and estrus was checked about 48 hours after the injection. Then they were treated with 4 straws of semen 3 times, with 12-hour intervals. Embryos were collected by a non-surgical method 7 days after the first artificial insemination. The cows were considered to have resumed ovarian cyclicity on the day of ovulation if followed by regular ovarian cycles. Seventy two point seven percentage of the cows(16/22) had normal resumption of ovarian cyclicity(resumption within 40 days after superovulation), and 27.3%(6/22) had delayed resumption(resumption did not occur until>40 days after superovulation). Delayed resumption Type II(first ovulation did not occur until ${\geq}40$ days after superovulation, i.e. delayed first ovulation 13.6%) were the most common types of delayed resumptions. The mean numbers of total ova from < 10 and $10{\leq}$ of corpora lutea(CL) was $7.8{\pm}1.8$ and $12.7{\pm}2.7$, respectively. The number of transferable embryos differed between < 10 and $10{\leq}$ CL was $5.4{\pm}1.3$ and $8.1{\pm}3.4$, respectively. Four point five percentage of the cows(1/22) did not resumption their ovarian cyclicity until 60 days after superovulation treatment. Diverse researches on the superovulation treatment method that is suitable for high-producing Holstein donor cows would contribute to preventing ovarian cyclicity disorder, as well as to the early multiplication of cows with superior genes by increasing the utilization value of donor cows.

• Journal of Korean Neurosurgical Society
• /
• v.51 no.6
• /
• pp.328-333
• /
• 2012

Objective : The aim of this study is to determine the association between the cerebrospinal fluid (CSF) biomarkers and inflammation, and the predictive value of these CSF biomarkers for subsequent shunt associated infection. Methods : We obtained CSF samples from the patients with hydrocephalus during ventriculoperitoneal (VP) shunt operations. Twenty-two patients were enrolled for this study and divided into 3 groups: subarachnoid hemorrhage (SAH)-induced hydrocephalus, idiopathic normal pressure hydrocephalus (INPH) and hydrocephalus with a subsequent shunt infection. We analyzed the transforming growth factor-${\beta}1$, tumor necrosis factor-${\alpha}$, vascular endothelial growth factor (VEGF) and total tau in the CSF by performing enzyme-linked immunosorbent assay. The subsequent development of shunt infection was confirmed by the clinical presentations, the CSF parameters and CSF culture from the shunt devices. Results : The mean VEGF concentration (${\pm}$standard deviation) in the CSF of the SAH-induced hydrocephalus, INPH and shunt infection groups was $236{\pm}138$, $237{\pm}80$ and $627{\pm}391$ pg/mL, respectively. There was a significant difference among the three groups (p=0.01). Between the SAH-induced hydrocephalus and infection groups and between the INPH and infection groups, there was a significant difference of the VEGF levels (p<0.01). However, the other marker levels did not differ among them. Conclusion : The present study showed that only the CSF VEGF levels are associated with the subsequent development of shunt infection. Our results suggest that increased CSF VEGF could provide a good condition for bacteria that are introduced at the time of surgery to grow in the brain, rather than reflecting a sequel of bacterial infection before VP shunt.

• Magazine of the Korean Society of Agricultural Engineers
• /
• v.30 no.2
• /
• pp.55-66
• /
• 1988

This study was conducted to investigate the various engineering properties and correlationshops among the soil constants of alluvial clayey deposits distributed in the bay of Asan and their results are summarized as follows : 1. Grain size distribution of soil was consisted of 12 % of clay, 46-73 % of silt, 2-23 % of sand, and as for the consistency characteristics, 26-36 % of liquid limit, 18-21 % of plastic limit and 6-16 % of plastic index, and so the soil belonging to as a lower plastic nonorganic clay, it's specific gravity was 2,66-2.70, and the location on the plastic chart was approximately above the A-line. Z The natural moisture content and unit weight were 30-43 % and 1.76-1.87 g I cm$_3$, respectively, and according to increment of natural moisture content, the unit weight was decreased, and the initial void ratio and degree of saturation were shown of 0,87-1119 and 92- 100 %, most of saturated. 3. Cone resistance value which was shown 2.4 - 6.5 kg / $cm^2$ was a little lower and it was increased with the depth of layer and shown the formular $q_c=0.7_z+1.32$. 4. Unconfined compression strength was about 0.18-0.43kg /$cm^2$, cu, 0.1-0.22kg / $\psi$, $2-6^{\circ}$ under uu-test condition of triaxial, and CCU, 0.08-0.3 kg/cm , $\psi$, $12-18^{\circ}$ under the condition of cu-test. 5. Pre-consolidation load of characteristics of consolidation was 0.4-0.8 kg / $cm^2$, compression index, about 0.17-0.33. 6. Liquid limit and plastic index were incresased with the increment of clay content but most of alluvial clay was appeared as a normal through non-activity clay soil shown more natural moisture content than liquid limit, and their relationship as follows : LL=0.38( cy+54.8), PI=0.836(LL -17.8), PI =0.468(LL -0.48) 7. The initial void ratio presented correlationship of positive among clay content, natural moisture content and liquid limit, and that of reverse with unit weight, and their results as follws : $e_o=0.024(w_n+0.2)$, $e_o=e_o=0.0003c_y+0.0005 LL+0.0151 W_n+\frac{3.58}{r-t}-1.52$ 8. It was shown that the compression index has correlationship of postive among the clay content, liquid limit, plastic index, natural moisture content and initial void ratio, and their relationships as follows ; $c_c=0.44(e_o-0.47)$, $c_c=0.001$

• Journal of Microbiology and Biotechnology
• /
• v.10 no.1
• /
• pp.27-34
• /
• 2000

The chloroform and methanol (2;1, v/v) extract from an edible plant, Actinidia arguta Planchon, appeared to possess antitumor activity against human leukemias Jurkat T and U937 cells through inducing apoptosis. The substance in the solvent extract was purified by silica gel column chromatography, preparative TLC, and Sephadex LH-20 column chromatography. Characteristics of the substance analyzed by UV scanning analysis, $^1H$ and $^{13}C$ NMR spectra suggested that the substance belongs to the chlorophyll derivatives-like group. The $IC_{50}$ value of the chlorophyll derivative (Cp-D) determined by MTT assay was $15\mu\textrm{g}/ml$ for Jurkat, $10\mu\textrm{g}/ml$ for U937, and $11.4\mu\textrm{g}/ml$ for HL-60m and was more toxic to these leukemias than to solid tumors or normal fibroblast. In order to elucidate cellular mechanisms underlying the cytotoxicity, the effect of the Cp-D on Jurkat T cells was investigated. When cells were treated with the Cp-D at a concentration of $15\mu\textrm{g}/ml$, [3H]thymidine incorporation declined rapidly and wa undetectable in 1h. However, no significant changes were made in the cell cycle distribution of the cells by 24h. The sub-Gl peak representing apoptotic cells began to be detectable in 36h, at which time apoptotic DNA fragmentation was also detected on agarose gel electrophoresis, demonstrating that the cytotoxic effect of the Cp-D is attributable to the induced apoptosis. Under the same conditions, although the protein level of cyclin-dependent kinases such as cdc4, csk6, cdk2, and cdc2 was not significantly changed until 24h, the kinase activity of all c안 rapidly declined and reached a minimum level within 1-6h and then recovered to the initial level by 12h and sustained until 24h. These results suggest that inactivation of cdks at an inappropriate time during the cell cycle progression in jurkat T cells following a treatment with the Cp-D leads to induction of apoptotic cell death.

• Clinical and Experimental Pediatrics
• /
• v.49 no.11
• /
• pp.1167-1173
• /
• 2006

Purpose : To evaluate various sepsis screening tests, individually and in combination, to formulate a guideline for the diagnosis of neonatal sepsis. Methods : The study was a retrospective cohort study. It took place at the neonatal intensive care unit of the Paediatric Department, Il Sin Christian Hospital, Busan, Korea, over a period of 68 months from 1st, April, 2001 to 31st, December, 2005. This study evaluated 100 neonates having clinical features of sepsis and 100 normal asymptomatic neonates and used screening tests including C-reactive protein (CRP), total leukocyte count (TLC), absolute neutrophil count (ANC), immature neutrophils to total neutrophil count ratio (I/T ratio), thrombocytopenia, degenerative changes in the neutrophils and gastric aspirate cytology (GAC) for the diagnosis of neontal sepsis. Results : The sensitivity of CRP and ANC was high. CRP had 86 percent sensitivity for group-A (proven sepsis) and 74 percent sensitivity for group-B (probable sepsis) and 94 percent specificity for group-A, B. ANC had sensitivity of 72 percent for group-A and 62 percent for group-B and 86 percent specificity for group-A, B. For group-A, sensitivity, specificity of GAC for polymorphs was 74 percent and 94 percent respectively. As for sensitivity, specificity of platelet count for group-A was 64 percent and 89 percent respecively. The sensitivity, specificity and predictive values (PV) of the individual tests and different test combinations were also calculated for group-A and B. Conclusion : For the detection of culture negative cases in neonatal sepsis, screening tests including CRP, TLC, ANC, thrombocytopenia, cytoplasmic vacuolization in the neutrophils and GAC for polymorphs have high sensitivity. A combination of three tests has higher sensitivity.

• Journal of Korean Neurosurgical Society
• /
• v.29 no.1
• /
• pp.78-86
• /
• 2000

Objective : Lumbar spinal instability occurs when normal biomechanics support in lumbar vertebrae interrupted. Despite the recent enthusiastic studies, the precise radiological assessment has not been fully established, yet. Therefore, we carefully studied our cases to analyze the radiologic findings in lumbar spinal instability. Patients and Methods : We have put together radiological analysis and assessment based on 38 patients who have been diagnosed and treated for lumbar spinal instabilities from June 1994 to December 1998, Patients who have been diagnosed and treated for trauma were excluded from study. Results : The outcomes are as follows : 1) Lumbar lordotic curve was statistically significant in unstable group by 23.7, compared to the control group ($17.0^{\circ}$). 2) According to the resting x-ray, sagittal plane angulation measured on unstable group was $21.1^{\circ}$, control group $18.0^{\circ}$. Therefore unstable group was noticeably higher(p<0.01). 3) According to the resting x-ray sagittal plane displacement, unstable group had 4.3mm, the comparison had 1.2mm. Therefore measurement from the unstable group were significantly higher(p<0.01). 4) According to stress view, sagittal plane translation was 4.1mm for the unstables and 2.7mm for the comparisons. Therefore unstables were noticeably higher(p<0.01). 5) According to stress view, sagittal plane rotation was $15.1^{\circ}$ at L3-4, $22.0^{\circ}$ at L4-5, $27.9^{\circ}$ at L5S1 for the unstable group and $11.3^{\circ}$, $18.1^{\circ}$, $21.0^{\circ}$ each for the comparison. 6) Facet angle for unstable group, left $29.3^{\circ}-61.5^{\circ}$, right $24.4^{\circ}-63.2^{\circ}$ and the mean for each are $43.1^{\circ}$, $47.2^{\circ}$. The difference between left and right facet angle was $3.5^{\circ}-20.7^{\circ}$ and the mean value $15.3^{\circ}$. Facet angle for the comparisons for the left was $29.3^{\circ}-59.5^{\circ}$, right was in between $25.7^{\circ}-64.5^{\circ}$ range and the each mean are $44.9^{\circ}$ and $47.6^{\circ}$. Also, the difference between left and right facet angle was $4.1^{\circ}-9.3^{\circ}$ and the average was $17.1^{\circ}$. The average and the difference between the left and right angle are found not to have statistic necessity for both unstable and stable measurements(p>0.01). 7) 19 patients were found to have vacuum facet phenomenon among unstable group etc. results were collected. Conclusion : According to above results, we attempted to prepare the application to the patient of radiological analysis and assessment for lumbar spinal instability early checkup.

• Journal of Nutrition and Health
• /
• v.14 no.2
• /
• pp.59-70
• /
• 1981

The objective of this study is to induce the primary characteristics of phenylketonuria in infant rats during the first 2 weeks after birth. The critical biochemical parameter in the development of phenylketonuria is the elevation of plasma phenylalanine while tyrosine is maintained at a relatively low level. A PKU-like condition was induced in infant rats during the first 2 weeks after birth using a modification of our previously published procedure for the development of a temporary (1 to 3 days) PKU-like condition. Phenylalanine was administered by stomach intubation every 6 hours (starting at 6:00 a.m.) at a dose level of 400mg per kg body weight (after birth-day 2 thru 5) and 500mg per kg body weight (day 6 thru 14). Amethopterin was given at 0.00625 or 0.0125mg per kg body weight (day 3 thru 14) and p-chlorophenylalanine at 50 mg per kg body weight (day 5 thru 14) at 9:00 a.m. and 9:00 p.m. At the times measured (6,10 and 14 days) plasma phenylalanine/tyrosine (P/T) ratios were elevated from a normal value of the or less to values ranging from 6 to 15. During the second week after birth a staggering gait, abnormal stance and decreased social behavior were also observed. None of these PKU-like characteristics were apparent in the three control groups receiving (a) no phenylalanine or inhibitors, (b) phenylalanine alone, or (c) inhibitors alone. The establishment of these primary biochemical characteristics of phenylketonuria by stomach intubation of phenylalanine and a combination of low dose levels of enzyme inhibitors to infant rats provides an experimental system which should he valuable for extensive biochemical, histological and behavioral studies in phenylketonuria.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.32 no.3
• /
• pp.353-358
• /
• 1999

This study is to find out the effects of various iron concentrations on growth, feeding and feed efficiency of olive flounder, Paralichthys olivaceus, The growth rate, feeding rate and feed efficiency of olive flounder in each experiments were represented by the relative value. The relative growth rate and relative feed efficiency of olive flounder were kept almost constant below the iron concentration of 1.0 mg/$\ell$, but were significantly decreased above the 5,0 mg/$\ell$ iron concentration. The growth rate, feeding rate and feed efficiency of olive flounder exposed to 10.0mg/$\ell$ iron concentration were significantly decreased than that exposed to normal condition. From these results, it could be concluded that the high level of 5.0 mg/$\ell$ iron concentration in the bottom water would curtail production of olive flounder in coastal area.