• Korean Journal of Acupuncture
• /
• v.33 no.2
• /
• pp.58-66
• /
• 2016

Objectives : In order to find a possible non-invasive manipulation tool for maintenance of the cardiovascular functions in hemorrhagic shock, this study was aimed at evaluating effects of acupoints acupressure on the changes in blood pressure and heart rate from an animal model of hemorrhagic shock. Methods : In adult Sprague-Dawley rats, hemorrhagic shock was induced by a withdrawal of arterial blood from the femoral artery with volume of 0.8 ml per 100 g of body weight using peristaltic syringe pump. We applied the acupressure with a pressure oscillator to tail as a control and 2 different acupoints of sobu(HT8), youngchun(KI1) under 3 different conditions : 1) normal arterial blood pressure without bleeding, 2) at the beginning of bleeding, and finally 3) hemorrhagic shock. Results : Under normal arterial blood pressure without hemorrhage, there was a significant increase in systolic and diastolic blood pressures by the acupressure to the tail, HT8 and especially KI1 for 30 sec compared with before acupressure. Under hemorrhagic shock condition, the tail acupressure had minimal changes in cardiovascular parameters. Either the HT8 or KI1 acupressure resulted in a significant increase in arterial pressure but did not heart rate. At the beginning of bleeding, tail acupressure failed to change the reduction of arterial pressure and heart rate. However, there was a significant increase in blood pressure and heart rate following either the HT8 or especially KI1 acupressure. Conclusions : HT8 and KI1 acupressure affected cardiovascular signs but tail acupressure did not in rat model of hemorrhagic shock. These experimental data suggest that a acupressure with a pressure oscillator to HT8 or KI1 can be one of alternative emergency manipulations to ameliorate compromised cardiovascular functions under hemorrhagic shock condition.

• Transactions of the Korean Society for Noise and Vibration Engineering
• /
• v.20 no.12
• /
• pp.1183-1189
• /
• 2010

Shock-resistance test for a real equipment for a normal vessel is one of the difficult problem in many cases because of terrible cost and weight. An analysis technique to evaluate the shock resistance in a design stage is necessary, instead In this paper, the process to evaluate the shock resistance of a propulsion motor for naval vessels was presented based on German navy's BV043 regulation. The shock signal to impose the equipment under the test was first evaluated, and was then applied to the structural FE model of the equipment. From the transient FEA, the time history of von-Mises stress was obtained by the mode superposition method. The shock resistance was evaluated using the peak value of the von-Mises stress.

• Asian-Australasian Journal of Animal Sciences
• /
• v.29 no.3
• /
• pp.419-427
• /
• 2016

There is a high association of heat shock on the alteration of energy and lipid metabolism. The alterations associated with thermal stress are composed of gene expression changes and adaptation through biochemical responses. Previous study showed that Angelica gigas Nakai (AGN) root extract promoted adipogenic differentiation in murine 3T3-L1 preadipocytes under the normal temperature condition. However, its effect in heat shocked 3T3-L1 cells has not been established. In this study, we investigated the effect of AGN root hot water extract in the adipogenic differentiation of murine 3T3-L1 preadipocytes following heat shock and its possible mechanism of action. Thermal stress procedure was executed within the same stage of preadipocyte confluence (G0) through incubation at $42^{\circ}C$ for one hour and then allowed to recover at normal incubation temperature of $37^{\circ}C$ for another hour before AGN treatment for both cell viability assay and Oil Red O. Cell viability assay showed that AGN was able to dose dependently (0 to $400{\mu}g/mL$) increase cell proliferation under normal incubation temperature and also was able to prevent cytotoxicity due to heat shock accompanied by cell proliferation. Confluent preadipocytes were subjected into heat shock procedure, recovery and then AGN treatment prior to stimulation with the differentiation solution. Heat shocked preadipocytes exhibited reduced differentiation as supported by decreased amount of lipid accumulation in Oil Red O staining and triglyceride measurement. However, those heat shocked preadipocytes that then were given AGN extract showed a dose dependent increase in lipid accumulation as shown by both evaluation procedures. In line with these results, real-time polymerase chain reaction (RT-PCR) and Western blot analysis showed that AGN increased adipogenic differentiation by upregulating heat shock protection related genes and proteins together with the adipogenic markers. These findings imply the potential of AGN in heat shock amelioration among 3T3-L1 preadipocytes through heat shock factor and proteins augmentation and enhanced adipogenic marker expression.

• Transactions of the Korean Society of Mechanical Engineers B
• /
• v.41 no.9
• /
• pp.615-622
• /
• 2017

A normal shock wave is initially formed in the shock tube that migrates towards the closed end of the tube, which, in turn, leads to the reflection of shock. Due to the interaction of the reflected shock with the boundary layer, bifurcation of the shock wave takes place. A shock train will be generated after the bifurcated shock wave approaches the contact surface. Until now, only a few studies have been conducted to investigate this shock train phenomenon inside the shock tube. For the present study, a CFD analysis has been performed on a two dimensional axisymmetric model of a shock tube using unsteady, compressible Navier-Stokes equations. In order to investigate the detailed characteristics of the shock train phenomenon, quantitative studies have been performed by varying shock tube length, diameter under fixed diaphragm, and pressure ratio inside a shock tube.

• Korean Journal of Bronchoesophagology
• /
• v.7 no.1
• /
• pp.24-28
• /
• 2001

Background and Objectives： Heat shock protein(HSP) 27 is a member of the small HSP family that plays a part in the epithelial cell growth and differentiation, wound healing. apoptosis and cell protection against inflammatory cytotoxic mediators. The expression of HSP27 was investigated in normal laryngeal tissue and squamous cell carcinoma of the larynx. Materials and Methods : We studied expression of HSP27 by Western blot on 20 patients of laryngeal squamous cell carcinoma. Results： HSP27 expressed in all normal and cancer tissues. In 9 cases(45%), expression of HSP27 more prominent in cancer tissue. Statistically, there were no significant difference in the expression of HSP27 in normal and cancer tissue, clinical stage and tumor differentiation. Conclusion 45% of cancer tissues was more prominent than normal tissue. But further studies on expression of HSP27 in laryngeal cancer and relationship with clinical parameter should be done.

• Asian-Australasian Journal of Animal Sciences
• /
• v.18 no.2
• /
• pp.192-196
• /
• 2005

A study was conducted to monitor the changes in serum protein profile, cholesterol and blood glucose during endotoxic shock in buffalo calves and also to assess the role of prophylactic supplementation of vitamin E and selenium in alleviating the endotoxic effects. Fifteen male buffalo calves (6-8 months of age) were divided into three groups: Group I (control)-infused with 0.9% saline solution; Group II-infused with E. coli endotoxin at 5${\mu}g/kg$ body weight in normal saline solution; Group III- supplemented prophylactically with 250 mg vitamin E and 7.5 mg selenium by i/m injections at weekly intervals for one month prior to the induction of endotoxic shock. The blood samples were collected at 0, 1, 3, 6, 9, 12, 24, 48 and 72 h after the induction of shock. Endotoxin caused a significant (p<0.05) hypoproteinemia from 3-12 h post infusion in group II but this hypoproteinemia was less pronounced and only from 3-9 h post infusion in vitamin E and selenium supplemented calves. Hypoglycemia was observed in group II from 3-24 h and blood glucose level returned to normal at 72 h. However hypoglycemia was mild in group III and blood glucose returned to normal at 48h. Hypocholesterolaemia and hypoalbuminemia were found in both groups II and III but these changes were less pronounced in group III i.e. vitamin E and Se supplemented calves. Serum electrophoretic protein patterns of group III were quite similar to those of control group but animals of group II had different electrophoretic pattern. It was concluded that the antioxidant effects of vitamin E and Se prevent the liver against oxidative stress during endotoxic shock.

• Journal of the Korean Society of Visualization
• /
• v.13 no.3
• /
• pp.29-34
• /
• 2015

The interaction between a normal shock wave and a boundary layer along a wall surface in internal compressible flows causes a very complicated flow. This interaction region containing shock train and mixing region is called as pseudo-shock waves. Pseudo-shock waves in the divergent part of a rectangular nozzle have been investigated by using large-eddy simulation (LES). LES studies have been done for the complex flow phenomena of three-dimensional pseudo-shock waves. The LES results have been validated against experimental wall-pressure measurements. The LES results are in good agreement with experimental results. Pseudo-shock length and corner separation have been studied in three-dimensional LES model. Comparison of centerline pressure measurement and 3D visualization measurement has been discussed for the corner separation position. It has been concluded that the pseudo-shock length should be measured by using 3D visualization measurement.

• Asian-Australasian Journal of Animal Sciences
• /
• v.21 no.2
• /
• pp.190-197
• /
• 2008

This study was designed to analyze boar sperm to compare motility, acrosome morphology, viability and ATP by various preservation methods between Duroc boar A with cold shock resistance sperm and Duroc boar B with cold shock sensitivity sperm. Semen volume, sperm concentration, motility and normal acrosome between Duroc boar A and B did not show any differences within 2 h after collection. There were no differences in sperm motility and normal acrosome between boar A and B at 1 day of preservation at $17^{\circ}C$ and $4^{\circ}C$, respectively. However, sperm motility and normal acrosome from 2 day of preservation at $17^{\circ}C$ and $4^{\circ}C$, respectively, were higher for boar A than boar B. The frozen-thawed sperm motility and normal acrosome were higher for boar A than boar B. The sperm viability and ATP concentration according to storage period of liquid semen at $17^{\circ}C$ and $4^{\circ}C$ were higher for boar A than boar B. Also, the sperm viability and ATP concentration of frozen-thawed semen were higher for boar A than boar B. In conclusion, we found out that the original quality of boar semen with cold shock resistance sperm played an important role.

• Biomolecules & Therapeutics
• /
• v.5 no.3
• /
• pp.223-227
• /
• 1997

The protective effect of human urinary trypsin inhibitor(UTI) on acute hemorrhagic shock in beagle dog was studied. Hemorrhagic shock was experimentally induced in thoracotomized beagle dogs by removing blood and maintaining low arterial blood pressure for 30 min, and then blood removed was entirely transfused back into the dogs within one hour. When the blood was transfused, UTI was administered together to check the potential protective effect of UTI on hemorrhagic shock. The arterial blood pressure recovery was accelerated slightly by UTI treatment. Blood pH and $P_{a co2}$ returned to normal level in shorter time in the UTI treatment group. These data suggest that UTI may have protective effects on experimentally induced hemorrhagic shock.

• Proceedings of the KSME Conference
• /
• 2001.11b
• /
• pp.456-461
• /
• 2001

This paper depicts the weak shock wave propagating inside some kinds of pipe bends. Computational work is to solve the two-dimensional, compressible, unsteady Euler Equations. The second-order TVD scheme is employed to discretize the governing equations. For the computations, the incident normal shock wave is assumed at the entrance of the pipe bend, and its Mach number is changed between 1.1 and 1.7. The turning angle and radius of the curvature of the pipe bend are changed to investigate the effects on the shock wave structure. The present computational results clearly show the shock wave reflection and diffraction occurring in the pipe bend. In particular, the vortex generation, which occurs at the edge of the bend, and its shedding mechanism are discussed in details.