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Two-Dimensional Shear Wave Elastography Predicts Liver Fibrosis in Jaundiced Infants with Suspected Biliary Atresia: A Prospective Study

  • Huadong Chen;Luyao Zhou;Bing Liao;Qinghua Cao;Hong Jiang;Wenying Zhou;Guotao Wang;Xiaoyan Xie
    • Korean Journal of Radiology
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    • v.22 no.6
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    • pp.959-969
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    • 2021
  • Objective: This study aimed to evaluate the role of preoperative two-dimensional (2D) shear wave elastography (SWE) in assessing the stages of liver fibrosis in patients with suspected biliary atresia (BA) and compared its diagnostic performance with those of serum fibrosis biomarkers. Materials and Methods: This study was approved by the ethical committee, and written informed parental consent was obtained. Two hundred and sixteen patients were prospectively enrolled between January 2012 and October 2018. The 2D SWE measurements of 69 patients have been previously reported. 2D SWE measurements, serum fibrosis biomarkers, including fibrotic markers and biochemical test results, and liver histology parameters were obtained. 2D SWE values, serum biomarkers including, aspartate aminotransferase to platelet ratio index (APRi), and other serum fibrotic markers were correlated with the stages of liver fibrosis by METAVIR. Receiver operating characteristic (ROC) curves and area under the ROC (AUROC) curve analyses were used. Results: The correlation coefficient of 2D SWE value in correlation with the stages of liver fibrosis was 0.789 (p < 0.001). The cut-off values of 2D SWE were calculated as 9.1 kPa for F1, 11.6 kPa for F2, 13.0 kPa for F3, and 15.7 kPa for F4. The AUROCs of 2D SWE in the determination of the stages of liver fibrosis ranged from 0.869 to 0.941. The sensitivity and negative predictive value of 2D SWE in the diagnosis of ≥ F3 was 93.4% and 96.0%, respectively. The diagnostic performance of 2D SWE was superior to that of APRi and other serum fibrotic markers in predicting severe fibrosis and cirrhosis (all p < 0.005) and other serum biomarkers. Multivariate analysis showed that the 2D SWE value was the only statistically significant parameter for predicting liver fibrosis. Conclusion: 2D SWE is a more effective non-invasive tool for predicting the stage of liver fibrosis in patients with suspected BA, compared with serum fibrosis biomarkers.

Characteristies of the background fabric and coloring of "Buseoksa Temple Gwaebul" in the possession of the National museum of Korea (국립중앙박물관 소장 <부석사 괘불>의 바탕직물과 채색 특성)

  • Park Seungwon;Yu Heisun;Park Jinho;Cheon Juhyun
    • Conservation Science in Museum
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    • v.31
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    • pp.1-20
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    • 2024
  • The "Buseoksa Temple Gwaebul" (1684, K969) in the possession of the National Museum of Korea is a large Buddhist hanging scroll produced for outdoor rituals (gwaebul) at the eponymous temple. The painting demonstrates the most complex composition among the existing Buddhist hanging scrolls as it depicts the Shakyamuni Buddha in the lower middle, surrounded by the Vairocana Buddha, Medicine Buddha, and Amitabha Buddha. This study examines the characteristics of the background fabric and the production methods of the scroll from Buseoksa Temple and explores the characteristics of the coloring techniques by integrating the results of a non-destructive analysis to determine the materials used for coloring. The gwaebul comprises a total of 13 panels, with 11 panels arranged side by side and one panel each added to the top and bottom. The background fabric of the painting consist of semi-transparent silk tabby for the nine panels in the center, and silk tabby for the four panels surrounding the four sides. The coloring materials used to paint the scroll were analyzed using X-ray fluorescence, and were confirmed to be inorganic pigments of red, yellow, green, blue, and white. For some parts painted in yellow and blue, the colors were expressed by first applying light white pigment before adding organic pigments. In addition, ink was used for the black lines and gold leaf was used for the patterns of the Buddhist robes. X-ray irradiation enabled the determination of the location and technique of coloring according to each pigment color by highlighting the difference in brightness depending on the main component and the thickness of each pigment.

Analysis of Water Surface Area Change in Reservoir Using Satellite Images (위성영상을 이용한 저수지 수체면적 변화 분석)

  • Kim, Joo-Hun;Kim, Dong-Phil
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.44 no.5
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    • pp.629-636
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    • 2024
  • The purpose of this study is to monitor changes in the water surface of reservoirs in verifiable areas in Korea using satellite images and to analyze the water surface area and water storage. The target area of this study is the Daecheong dam of the Geumgang(Riv.), which supplies water to some areas in the Chungcheong area. A study was conducted to detect water surface area by using the Sentinel-1(SAR-C) image and the optical image of Sentinel-2(MSI) among the various observation sensors of satellite images. The correlation between the reservoir's water storage volume, which is ground measurement data, and the extracted water surface area was analyzed. As a result of the analysis, the coefficient of determination(R2) between water surface area and daily storage using SAR images was analyzed to be 0.9242, and in the analysis using Sentinel-2's MSI optical image, it was analyzed to be correlated at 0.8995. In addition, it is analyzed that the water storage volume of the water surface area extracted from the image using the relationship between the water storage volume and the water surface area represents a hydrograph similar to the actual water storage volume. This study is a basic study for the use of satellite images in unmeasured/non-access areas such as North Korea, and plans to conduct a study to analyze annual changes and long-term trends in major dam reservoirs in North Korea by reflecting the results obtained through this study.

Studies on the Hematology and Blood Chemistry of Korean Cattle Part II. Studies on the Blood Chemistry of Korean Cattle (한국성우(韓國成牛)의 혈액학치(血液學値) 및 혈액화학치(血液化學値)에 관한 연구(硏究) 제2보(第二報) 한국성우(韓國成牛)의 혈액화학치(血液化學値)에 관한 연구(硏究))

  • Cheong, Chang Kook
    • Korean Journal of Veterinary Research
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    • v.5 no.1
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    • pp.97-123
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    • 1965
  • Observations were made on the blood picture of total 196 heads of healthy Korean cattles, including 98 males and females in the purpose of determination of blood chemical values and their sex differences and seasonal variations during one year period from December, 1963 to November, 1964. The blood sampling were scheduled by random in four different seasons and the sample size of both sex included in each season were designated to be same size. The ranges, averages or mean values of the blood glucose, total serum protein, serum globulin, serum albumin, total non-protein nitrogen, blood urea nitrogn, total serum cholesterol, serum inorganic phosphorus and serum calcium were determined in this studies and their respective standard deviation, standard error of means, sex differences and seasonal variations were as follows. 1. The blood glucose values for the male ranged from 32.8 to 70.0 mg/100cc. with a mean of $49.781{\pm}0.823mg/100cc$; for the female the range was 32.0 to 64.0mg/100cc. with a mean of $47.235{\pm}0.782mg/100cc$. Sex difference showed significant at 5% level and seasonal variation was highly significant at 1% level. 2. The total serum protein values for the male ranged from 5.61 to 8.83 gm/100cc with a. mean of $7.366{\pm}0.062gm/100cc$; for the female ranged from 5.53 to 8. 43 gm/100cc. with a mean of $6.832{\pm}0.063gm/100cc$. Sex difference and seasonal variation was not significant. 3. The serum globulin values for the male ranged from 2.97 to 4.78 gm/100cc. with a mean of $3.961{\pm}0.039gm/100cc$.; for the female ranged from 2.87 to 4.41 gm/100cc. with a mean of $3.699{\pm}0.037gm/100cc$. Sex difference showed highly significant at 1% level and seasonal variation was not significant. 4. The serum albumin values for the male ranged from 2.58 to 4.21 gm/100cc. with a mean of $3.405{\pm}0.029gm/100cc$.; for the female ranged from 2.39 to 4.10 gm/100cc. with a mean of $3.204{\pm}0.031gm/100cc$. Sex difference showed highly significant at 1% level and seasonal variation was not significant. 5. The total non-protein nitrogan values for the male ranged from 19.1 to 44.8 gm/100cc. with a mean of $31.166{\pm}0.582mg/100cc$.; for the female the range was 15.2 to 50.5 mg/100cc. with a mean of $28.89.6{\pm}0.673mg/100cc$. Sex difference showed significant at 5% level and seasonal variation was highly significant at 1 % level. 6. The blood urea nitrogen values for the male ranged from 6.4 to 28.3 mg/100cc. with a mean of $13.371{\pm}0.466mg/100cc$.; for the female the range, was 6.0 to 26.9 mg/100cc. with a mean of $13.631{\pm}0.321mg/100cc$. Sex difference was not significant and seasonal variation showed highly significant at 1 % level. 7. The total serum cholesterol values for the male ranged from 60.0 to 238.6 mg/100cc. with a mean of $140.897{\pm}2.826mg/100cc$.; for the female ranged from 50.0 to 243.0 mg/100cc. with a mean of $124.840{\pm}3.553mg/100cc$. Sex difference and seasonal variation showed highly significant at 1% level. 8. The serum inorganic phosphorus values for the male ranged from 3.5 to 7.8 mg/100cc. with a mean of $5.426{\pm}0.096mg/100cc$.; for the female ranged from 3.1 to 8.8 mg/100cc. with a mean of $5.570{\pm}0.128mg/100cc$. Sex difference and seasonal variation showed no significant. 9. The serum calcium values for the male ranged from 7.8 to 12.8 mg/100cc. with a mean of $10.761{\pm}0.102mg/100cc$.; for the female ranged from 8.0 to 13.0 mg/100cc. with a mean of 10. $756{\pm}0.097mg/100cc$. Sex difference was not significant and seasonal variation showed highly significant at 1% level. 10. The age of test group ranged from 2 years to 6 years in both sex and the averageage were, $4.45{\pm}0.114$ years in male and $4.50{\pm}0116$ years in female. Sex difference and seasonal variation of age were not found to be significant.

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Studies on the Hematology and Blood Chemistry of Korean Cattle Part I. Studies on the Hematology of Korean Cattle (한국성우(韓國成牛)의 혈액학치(血液學値) 및 혈액화학치(血液化學値)에 관한 연구(硏究) 제1보(第一報) 한국성우(韓國成牛)의 혈액학치(血液學値)에 관한 연구(硏究))

  • Cheong, Chang Kook
    • Korean Journal of Veterinary Research
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    • v.5 no.1
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    • pp.61-96
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    • 1965
  • Observations were made on the blood picture of total 196 heads of healthy Korean cattles, including 98 males and 98 females in the purpose of determination of hematological values and its sex difference, and seasonal variations during one year period from December 1963 to November 1964. The blood sampling were scheduled by random in four different seasons and the sample size of both sex included in each season were designated to be same size. The ranges, averages or mean values of the erythrocytes, hemoglobin, hematocrit, mean corpuscular hemoglobin concentration, total white blood cell count and differential count were determined in this studies and their respective standard deviation, standard error of means, sex defferences and seasonal variations were as follows; 1. The erythrocyte count of male showed a range of $5.0{\times}10^6/c.mm$ to $8.75{\times}10^6/c.mm$ with a mean of $6.5{\pm}0.096{\times}10^6/c.mm$. Female showed a range of $5.0{\times}10^6/c.mm$ to $8.30{\times}10^6/c.mm$, with a mean of 6. $131{\pm}0.078{\times}10^6/c.mm$. There was a highly significant sex difference and seasomal variation was not found to be significant. 2. The hemoglobin value of male showed a range of 9.0g/100cc. to 14.5g/100cc. with a mean of $11.074{\pm}0.143g/100cc$. Female showed a range of 9.0g/100cc to 13.0g/100cc. with a mean of $10.745{\pm}0.034g/100cc$. There was a highly significant sex difference and seasonal variation was not found to be significant. 3. The hematocrit value of male showed a range of 28% to 45% and with a mean of $34.867{\pm}0.468%$. Female showed a range of 28% to 42% with a mean of $32.888{\pm}0.322%$. There was a highly significant sex difference and seasonal variation was not found to be significant. 4. The mean corpuscular hemoglobin of male showed a range of 14.4rr. to 19.6rr. with a mean of $17.1{\pm}0.112rr$. Female showed a range of 14.7rr. to 19.5rr. with a mean of $17.6{\pm}0.113rr$. 5. The mean corpusular volume of male showed a range of $42.5{\mu}^3$ to $62.2{\mu}^3$ with a mean of $53.9{\pm}0.419{\mu}^3$, Female showed a range of $44.2{\mu}^3$ to $60.0{\mu}^3$ with a mean of $53.8{\pm}0.375{\mu}^3$. 6. The mean corpuscular hemoglobin concentration of male showed a range of 28.1 % to 34.9% with a mean of $31.4{\pm}0.161%$. Female showed a range of 28.0% to 34.9% with a mean of $30.9{\pm}0.169%$. 7. The total leucocyte count of male showed a range of 4,000/c.mm to 13,100/c.mm. with a mean of $9,338{\pm}218.23/c.mm$. Female showed a range of 4,000/c.mm. to 14,000/c.mm. with a mean of $9,338{\pm}235.90/c.mm$. Six difference was not found to be significant and there was a highly significant seasonal variation. 8. The differential count of male, the means of neutrophil, stab, segmented cell, Iymphocyte, monocyte, eosinophil and basophil were $31.173{\pm}0.570%$, 0.3%. $30.867{\pm}0.564%$, $55.112{\pm}0.603%$, $3.745{\pm}0.082%$, $9.867{\pm}0.422%$ and 0.14% rspectively. Female showed means of $31.010{\pm}0.572%$, 0.2%, $30.806{\pm}0.569%$, $53.929{\pm}0.634%$, $4.082{\pm}0.109%$, $10.908{\pm}0.503%$ and 0.12% respectively. There were significant sex differences in monocyte and highly significant sex difference in eosinophil, and seasonal variation were found to be highly significant in neutrophil, monocyte and eosinophil. 9. Hematological comparison made between cattles infested with so called "small type piroplasma" and non-infested group. The result of investigation showed no significant difference upon the red blood cell, hemoglobin and hematocrit values between lighty infested group and non-infested group. 10. Age distribution of test group in this study ranged from 2 years to 6 years in both sex and their average age were $4.45{\pm}0.114$(male) and $4.50{\pm}0.116$(female). There found to be no significant sex difference and seasonal variations in the age of test group.

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Actual Evapotranspiration of Sesame Crop Cultured With and Without Transparent Plastic Film Mulch (투명(透明) 프라스틱 필름 피복(被覆)에 따른 참깨의 실증발산량(實蒸發散量) 변화(變化))

  • Oh, Dong-Shin;Kwon, Yong-Woong;Im, Jung-Nam;Ryu, Kwan-Shig
    • Korean Journal of Soil Science and Fertilizer
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    • v.29 no.1
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    • pp.34-43
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    • 1996
  • Determining the actual evapotranspiration(ETa) of a crop, and appropriate water management of the crop based on the ETa are very important For increasing the yield. The present study aimed at determining ETa and crop coefficient of sesame growing under different climatic conditions with the transparent thin polyethylene film mulch(0.03 mm thick) and without this mulch. Bottomless cylindrical lysimeters(105cm in diameter, 120cm in height, protruded 20 cm above the soil surface) were installed on the field of sandy loam "Bonyang series" soil with a moderate drainage. The determination of ETa was performed by measuring each component of a model equation, $ETa=(R+I)-\{Ro+(D1+D2)\}+C{\pm}{\Delta}S$. Sesame, cv. "Ansan" was sown in two rows with the spacing of $50{\times}15cm$ on May 10 in 1991 and 1992. The mulching covers 80% of the soil surface. Sesame consumed the water of 139.0 mm(1.53 mm/day) and 171.2 mm(1.59 mm/day) in ETa without the film mulch, but that of 132.6 mm(1.46 mm/day) and 199.8 mm(1.85 mm/day) with its mulching through both years of 1991 and 1992, respectively. The ETa's accounted for 52 and 69% of the potential evapotranspiration(ETp) in the mulched crop, and 54 and 59% of ETp in the non-mulched crop 1991 through 1992, respectively. Its ETa's were much more and their gap between the mulching and non-mulching treatment was larger in 1992 than in 1991 as a result of the better climatic condition of 1992.

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The Changes of Mortality Differentials by Socioeconomic Determinats(1970~86) : Based on Death Registration Data (사회$\cdot$경제적 요인별 차별 사망력의 변화: 1970 ~ 1986)

  • 윤덕중;김태헌
    • Korea journal of population studies
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    • v.12 no.2
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    • pp.1-21
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    • 1989
  • For the analysis of mortality differentials by socioeconomic factors based on death registration data, we have considered four variables : place of residence, educational attainment, marital status and occupation. The age range adopted were 5 to 64 years of age for place of residence, and 25 to 64 years of age for the other factors. The mortality differentials by socioeconomic variables were clear and in the expected direction: mortality levels among urban residents, better educated groups, and non- agricultural workers were lower than among the other sub- groups. The average mortality level in rural areas is much higher than in urban areas : the rural mortality levels were at least double the urban levels at ages below 40 years, but became smaller after age 40, and no clear differentials by urban I rural residence increased until 1974~76 for the both sexes, but since the then differentials have declined slowley for both sexes. This changing pattern of mortality differentials by place of residence can be explained by historical socioeconomic development : the development generally started in urban areas, and rural areas followed : in the course of socioeconomic development the differences between the death rates in the two areas became smaller and finally the mortality levels in the two areas became nearly the same, as is found in the developed countries nowadays. The inverse relationships between mortality and educational level became stronger between the periods 1970~72 and 1984~86, but showed the same atterns of mortality differentials in both period : larger differences among the younger age groups, and for males, than among the older age groups, and for females. The increasing mortality differentials in the fourteen-year period between 1970~72 and 1984~86 were caused by inadequate living standards of the non- educated, whose proportion in the total population, however, dropped sharply during that period. Also, the much lower proportions of low - educated groups or of persons with no formal education among males than females helped to establish the clearly pronounced differentials. The mortality differentials by marital status in Korea showed the usual pattern : the mortality rates of the married in each age and sex group were clearly lower than those of others during the fourteen-year period between 1970~72 and 1984~86. In Korean society which remotes universal marriage, the never married recorded especially high death rates, presumably mainly because of ill - health, but also possibly because of the stigma attached to celibacy. However, the mortality differentials by marital status changed with the changes in the proportionate distribution by marital status during the period : the differences between the death rates of the married and never married groups became smaller, the proportion of the never married group increased : in contrast, the differences between mortalities of the married and widowed / divorced / separated groups widened, with the decrease in the proportion of the later group ; this tendency was perticularly marked for females. Occupational groups also showed clear mortality differences : among four occupational groups mortality of males was highest among agricultural workers and lowest among 'professional, admi-nistrative and clerical workers, However, when the death rates were standardized by educational level, the death rates by occupation in age group 45~64 years were nearly the same (excet for the mixed group consisting of unemployed, students, military servicemen and unknown). Therefore, the clear mortality dfferentials by occupation in Korea resulted mainly from the differences in educational level between different occupation groups. Since socioeconomic characteristics are related to each other, the net effect of each variable was examined. Each of the three variables - ducational level, marital status and urban / rural residence affected significantly Korean adult mortality when the effects of the other variables were controlled. Among the three variables educational level was the most important factor for the determination of the adult mortality level. When male's occupation was added to the above three variables, the effects of occupation on adult mortality were notably smaller after control for the effects of the other three variables while the net effects of these three variables were nearly the same irrespectively whether occupation was included or not. Thus, the differences in educational level (mainly), place of residence and marital status bring out the clear differences in observed mortality levels by occupation.

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Age Determination by Tooth Wear and Histological Analysis of Seasonal Variation of Breeding in the Lesser White-Toothed Shrew, Crocidura suaveolens (작은땃쥐 Crocidura suaveolens의 치아 마모에 의한 연령결정과 번식의 계절적 변이의 조직학적 분석)

  • Jeong, Soon-Jeong;Yoon, Myung-Hee;Kim, Sook-Hyang;Ham, Joo-Hyun;Lim, Do-Seon;Choi, Baik-Dong;Park, Jin-Ju;Jeong, Moon-Jin
    • Applied Microscopy
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    • v.40 no.3
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    • pp.125-132
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    • 2010
  • Captured specimens of the lesser white-toothed shrew, Crocidura suaveolens were classified into three age classes by tooth wear and seasonal variations of reproductive organs were investigated. Molars of juveniles had not tooth wear and the height of the third molars were lower than the first and second molars, young adults had smooth tooth wear and the third molars reached to the first and second molars, and old adults had heavy tooth wear and the third molars also reached to the first and second molars. On the basis of histological examination, seasonal variation of breeding was confirmed that breeding season of adult males was from early February to early October, having a peak of the breeding in April and July, and non-breeding season was from in the middle of October to late January. Young and old adult males of the breeding season had large testes with enlarged seminiferous tubules filling with numerous germ cells and expanded caudal epididymides with a vast number of spermatozoa, Young and old adult males of the non-breeding season had the small testes with the extremely slender seminiferous tubules filling with only spermatogonia and the reduced caudal epididymides without spermatozoa. Males weighing more than 3.9 g in the body weight and 0.013 g in the testis and epididymis weight reached sexual maturation in breeding season, and the females weighing more than 3.8 g in body weight of the breeding season were pregnant condition having 5~6 litters or had the Graafian follicles and the corpus lutea in the ovary.

A Study On Irrigation Water Price Structure and Prescription (농업용수의 가격구조에 관한 연구)

  • 심기영
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.15 no.4
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    • pp.3170-3180
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    • 1973
  • This study of the subject will review past and present irrigation development in Korea. Particular attention will be given to water pricing structure and a case study on the purpose of rational operation and management of irrigation water and organizations, and the optimum irrigation water and organizations, and the optimum irrigation water fee inorder to reduce farmers burden and to rationalize the farmland associations management so as to achieve development of the rural environment. In 1971, the reservoir of the Farmland Improvement A sociation (FIA) produced only 775 millison $m^3$ of irrigation water or 77% of planned capacity of 1,015 million $m^3$. It was caused by inefficient maintenance of irrigation facilities; for instance, about 21% of reservoirs, pumping stations and weirs in Korea have been silted by soil erosion which hinder to water production according to an ADC survey. The first Irritation Association was established in 1906, whcih was renamed the Farmland Assoeiation by the Rural Development Enouragement Law in 1970. By the end of 1971, 411,000 ha of rice paddies were under the control of 267 associations nationwide. The average water price assessed by Associations nationwide rose from 790 won per 0.1 ha. in 1966 to 1,886 won in 1971. The annual growth rate was 20%. The highest water price in 1971 was 4,773 won her 0.1 ha. and the lowest was 437 won. This range was caused by differences in debt burden, geographic conditions and management efficiency among the Associations. In 1971, the number of Associations which exceeded the average water price of 1,886 won per 0.1 ha. was 144, or 55.1% of all Association. In determination of water price, there are two principles; one is determined by production cost such as installation cost of irrigation facilities, maintenance cost, management cost and depreciation ect. For instance, the Yong San River Development project was required 33.7 billion won for total construction and maintenance cost is 3.1 billion won for repayment, maintenance and management cost per year. The project produces 590 million $m^3$ of irrigation water annually. Accordingly, the water price per $m^3$ is 5.25 won. The other principle is determined by water value in the crop products and in compared with production of irrigated paddy and non-irrigated paddy. By using this method, water value in compared with paddy rice vs. upland rice(Average of 1967-1971) was 14.15 won per $m^3$ and irrigated paddy vs. non-irrigated paddy was 2.98 won per $m^3$. In contrast the irrigation fee in average association of 1967-1971 was 1.54 won per $m^3$. Accordingly, the current national average irrigation fee(water price) is resonable compared with its water value. In this study, it is found that the ceiling of water price in terms of water value is 2.98 won per $m^3$ or 2,530 won per 0.1 ha. However, in 1971 55% of the associations were above the average of nationwide irrigation fees. which shows the need for rationalization of the Association's management. In connection with rationalization of the Association's management, this study recommends the following matters. (1) Irrigation fee must be assessed according to the amount of water consumption taking intoaccount the farmer's ability. (2) Irrigation fee should be graded according to behefits and crop patterns. (3) Training personnel in the operation and procedures of water management to save O&M costs. (4) Insolvent farmland association should be integrated into larger, sound associations in the same GUN in order to reduce farmers' water cost. (5) The maintenance and repair of existing irrigation facilities is as important as expansion of facilities. (6) Establishment of a new Union of Farmland Association is required to promoted proper maintenance and to protect the huge investment in irrigation facilities by means of technical supervision and guidance.

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A Study on Red Cell Protoporphyrin Concentration and Iron Metabolism (적혈구(赤血球) Protoporphyrin과 철분대사(鐵分代謝)에 관(關)한 연구(硏究))

  • Cho, Kyung-Hwan;Tchai, Bum-Suk
    • Journal of Nutrition and Health
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    • v.7 no.3
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    • pp.1-13
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    • 1974
  • The relative state of human iron storage may be ascertained more reliably through determination of the serum iron, iron binding capacity, transferrin saturation and absorption of radioactive iron in conjunction with studies of red cell morphology than from the study of red cell morphology alone. Recent investigations have shown that there is an increase in red cell protoporphyrin concentration in iron deficiency anemia. The significance of the red cell protoporphyrin has been discussed greatly during the years since its discovery. Two of the main factors which appear to influence the amaunt of protoporphyrin are increased erythropoiesis and factors interfering with the utilization of iron in the synthesis of hemoglobin, and iron deficiency. Recently Heller et al. have described a simplified method for blood protoporphyrin assay and this technique could be used assess nutritional iron status, wherein even minor insufficiencies are detectable as increased protoporphyrin concentrations. Based on the evaluation of the relationship between nutritional iron status and red cell protoporphyrin as an index suitable for the detection of the iron deficiency is described in this paper. RESULTS 1. Hemoglobin Concentrations and Anthropometric Measurements. The mean and standard deviations of the various anthropometric measurements of different age and sex groups are shown in table 1. There measurements have been compared with the Korean Standard. In the absence of local standards for arm circumference and skin-fold thickness over triceps, they have been compared with the standard from Jelliffe. Table 2,3, and 4 give anthropometric measurements and frequency (%) of anemia in children surveyed. The mean height of the children studid was 10 to 20 percent; below the Korean Standard. The distribution of height below 80 percent of the Standard was 21.2 percent, however, among anemic group this percentage was 27.7 percent. In general, the mean weight of the children was 10 to 15 percent below the Korean Standard. The percentage of children with weight less than 80 percent of the Standard was about 35 percent. But in the anemic group of the children, this percentage was 44 percent. The mean arm circumference was about 15 percent lower than the Jelliffe's standard. 61.2 percent of the children had values of arm circumference below 80 percent of the standard. Children with low hemoglobin levels, this percentage was 80 percent. The mean skinfold thickness over the triceps of the children studied was about 25 Percent lower than the Jelliffe's standard and 61.2 percent of the children had the value less than 80 percent of the standard. Among anemic children, this percentage was 70.8%. As may be seen from table 5, the mean hemoglobin concentration of the total group was 11.3g/100ml. Hemoglobin concentration was less than 11.0g/100ml. in 65(36.5%) of the 178 children. The degree of anemia in most of these children was mild with a hemoglobin level of less than 8.0g/100ml. found in only one child. In general, the prevalence of anemia was high in female children than male and decreased its frequency with increasing age. Relatively close relationship was observed between hemoglobin level and anthrophometric measurements especially high between arm circumference and skinfold thickness and hemoglobin but very low in height and low in weight and hemoglobin level, estimated by chi-square value. II. Serum iron, Transferrin saturation (1) Serum iron, and transferrin saturation Serum iron, transferrin saturation and red cell protoporphyrin concentrations were estimated in sub-sample of 84 children from 1 to 6 years and 24 older children between 7 and 13 years of age. The findings are presented in table 6. The mean serum iron concentration of the total group was 59ug/100ml. However, the level incrased with age from 36.6ug/100ml. (1-3years) to 80.8ug/100ml. (7-13 years). 60 percent of these children had a serum iron level less than 50ug/10ml. in the 1-3 years age group and 31.4 percent for 4-6 years group. These contrast with the finding of 12.5 percent anemic children in the 7-13 years age group. The mean transferrin saturation for the total group was 18.1 percent and frequency of anemia by transferrin saturation was observed same pattern as serum iron concentration. (2) Red cell protoporphyrin concentrations. (a) Red cell protoporphrin levels of children: Red cell protoporphyrin and other biochemical data are shown in table 4. The mean concentration in red cell of all children was fround 46.3ug/100ml. RBC. and differences with age groups were observed; in the age group 1-3 years, the mean concentration was $59.5{\pm}32.14$ ug/100ml. RBC; 4-6 years $44.1{\pm}22.57$ ug/100ml. RBC. and 7-13 years, $39.0{\pm}13.56$ ug/100ml. RBC. (b) Normal protoporphyrin values in adults: It was observed that in 10 normal adult males studied here the level of protoporphyrin in red cell ranged from 18 to 54 ug/100ml. RBC. and the mean concentration was $47.5{\sim}14.47$ ug/100ml. RBC. Other biochemical determination made on the same subjects are presented in table 8. (c) Red tell protoporphyrin concentration of occupational blood donors: The results of analyses for red cell protoporphyrin as well as serum iron, transferrin saturation and hemoglobin in the 76 blood donors are presented in table 7 and 8. In this experiment, donors were selected at random, however, most of them bled repeatedly because of poor economic situation, I doubt. Table 9 shows the distribution of red cell protoporphyrin concentration and hemoglobin concentration of occupational donors. The mean hemoglobin value for the total was 11.9 g/100 ml. When iron deficiency anemia is defined as a transferrin saturation below 15%, prevalence of anemia was 47.4 percent and the mean serum iron was 27.1ug/100ml. and red cell protoporphyrin, 168.3ug/100ml. RBC. However, mean serum iron and protoporphyrin concentration of above 15% transferrin saturation were 11.6 ug/100 ml. and 58.8 ug/100 ml. RBC. respectively. The mean Protoporphyrin concentration of non-anemic (above 15% transferrin saturation) donors was slightly higher than the results of normal adult males.

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