• Title/Summary/Keyword: Non-Dominant

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Expression of γ-Tocopherol Methyltransferase Transgene Improves Tocopherol Composition in Lettuce (Latuca sativa L.)

  • Cho, Eun Ae;Lee, Chong Ae;Kim, Young Soo;Baek, So Hyeon;de los Reyes, Benildo G.;Yun, Song Joong
    • Molecules and Cells
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    • v.19 no.1
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    • pp.16-22
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    • 2005
  • A cDNA encoding ${\gamma}-tocopherol$ methyltransferase (${\gamma}-TMT$) from Arabidopsis thaliana was overexpressed in lettuce (Latuca sativa L.) to improve the tocopherol composition. Seven lines of lettuce ($T_0$) containing the ${\gamma}-TMT$ transgene were produced by Agrobacterium-mediated transformation. The inheritance and expression of the transgene were confirmed by DNA and RNA gel blot analyses as well as quantification of tocopherols and ${\gamma}-TMT$ activities. The ratio of ${\alpha}-/{\gamma}-tocopherol$ content (TR) varied from 0.6 to 1.2 in non-transformed plants, while the $T_0$ plants had ratios of 0.8 to 320. The ratio ranged from 0.4 to 544 in 41 $T_1$ progenies of the $T_0$ transgenic line gTM3, and the phenotypic segregation indicated monogenic inheritance of the transgene (i.e., 3:1 = dominant:wild-type classes). There was a tight relationship between the TR phenotype and ${\gamma}-TMT$ activity, and enzyme activities were affected by the copy number and transcript levels of the transgene. The TR phenotype was stably expressed in $T_2$ progenies of $T_1$ plants. The results from this study indicated that a stable inheritance and expression of Arabidopsis ${\gamma}-TMT$ transgene in lettuce results in a higher enzyme activity and the conversion of the ${\gamma}-tocopherol$ pool to ${\alpha}-tocopherol$ in transgenic lettuce.

Assessment of DMS photochemistry at Jeju Island During the Asian Oust-Storm Period of Spring 2001 : Comparison of Model Simulations with Field Observations

  • Shon, Zang-Ho;Hilton Swan;Keith N. Bower;Kim, Ki-Hyun;Lee, Gangwoong;Kim, Jiyoung
    • Proceedings of the Korea Air Pollution Research Association Conference
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    • 2002.11a
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    • pp.343-343
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    • 2002
  • This study examines the influence of long-range transport of dust particles and air pollutants on both local/regional DMS oxidation chemistry and the distribution of sulfur compounds at Jeju Island (33.17$^{\circ}$ N. 126.10$^{\circ}$ E) during the Asian dust-storm(ADS) period in April 2001. The atmospheric concentrations of these sulfur species were measured at a ground station on Jeju Island. Korea as Part of the ACE-Asia intensive operation. Three ADS events were observed during the periods of 10-12, 13-14. and 25-26 April. respectively. The concentrations of DMS and CS$_2$ were higher during the ADS period than during the non-Asian-dust-storm (NADS) period. Conversely. a difference in SO$_2$ levels during the ADS period was not distinguishable from those during the NADS period. The diurnal variation pattern of DMS observed was largely different from that in the remote marine boundary layer. DMS loss by NO$_3$ in the atmospheric boundary layer was dominant due to significantly high NOx levels influenced by the long-range transport of pollutants from East Asia to Jeju Island The DMS maximum during the ADS period was observed in the late afternoon. The oceanic fluxes of DMS during the ADS and NADS periods were estimated to be 5.7$\pm$2.3 and 2.9 (+2.8/-1.5) mole m$^{-2}$ day$^{-1}$ . respectively. The contribution of oxidized DMS to SO$_2$ levels at Jeju Island during the study period was found to be insignificant.

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Ramlibacter ginsenosidimutans sp. nov., with Ginsenoside-Converting Activity

  • Wang, Liang;An, Dong-Shan;Kim, Song-Gun;Jin, Feng-Xie;Kim, Sun-Chang;Lee, Sung-Taik;Im, Wan-Taek
    • Journal of Microbiology and Biotechnology
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    • v.22 no.3
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    • pp.311-315
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    • 2012
  • A novel ${\beta}$-proteobacterium, designated BXN5-$27^T$, was isolated from soil of a ginseng field of Baekdu Mountain in China, and was characterized using a polyphasic approach. The strain was Gram-staining-negative, aerobic, motile, non-spore-forming, and rod shaped. Strain BXN5-$27^T$ exhibited ${\beta}$-glucosidase activity that was responsible for its ability to transform ginsenoside $Rb_1$ (one of the dominant active components of ginseng) to compound Rd. Phylogenetic analysis based on 16S rRNA gene sequences showed that this strain belonged to the family Comamonadaceae; it was most closely related to Ramlibacter henchirensis $TMB834^T$ and Ramlibacter tataouinensis$TTB310^T$ (96.4% and 96.3% similarity, respectively). The G+C content of the genomic DNA was 68.1%. The major menaquinone was Q-8. The major fatty acids were $C_{16:0}$, summed feature 4 (comprising $C_{16:1}$ ${\omega}7c$ and/or iso-$C_{15:0}$ 2OH), and $C_{17:0}$ cyclo. Genomic and chemotaxonomic data supported the affiliation of strain BXN5-$27^T$ to the genus Ramlibacter. However, physiological and biochemical tests differentiated it phenotypically from the other established species of Ramlibacter. Therefore, the isolate represents a novel species, for which the name Ramlibacter ginsenosidimutans sp. nov. is proposed, with the type strain being BXN5-$27^T$ (=DSM $23480^T$ = LMG $24525^T$ = KCTC $22276^T$).

Human Postural Dynamics in Response to the Horizontal Vibration

  • Shin Young-Kyun;Fard Mohammad A.;Inooka Hikaru;Kim Il-Hwan
    • International Journal of Control, Automation, and Systems
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    • v.4 no.3
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    • pp.325-332
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    • 2006
  • The dynamic responses of human standing postural control were investigated when subjects were exposed to long-term horizontal vibration. It was hypothesized that the motion of standing posture complexity mainly occurs in the mid-sagittal plane. The motor-driven support platform was designed as a source of vibration. The AC Servo-controlled motors produced anterior/posterior (AP) motion. The platform acceleration and the trunk angular velocity were used as the input and the output of the system, respectively. A method was proposed to identify the complexity of the standing posture dynamics. That is, during AP platform motion, the subject's knee, hip and neck were tightly constrained by fixing assembly, so the lower extremity, trunk and head of the subject's body were individually immovable. Through this method, it was assumed that the ankle joint rotation mainly contributed to maintaining their body balance. Four subjects took part in this study. During the experiment, the random vibration was generated at a magnitude of $0.44m/s^2$, and the duration of each trial was 40 seconds. Measured data were estimated by the coherence function and the frequency response function for analyzing the dynamic behavior of standing control over a frequency range from 0.2 to 3 Hz. Significant coherence values were found above 0.5 Hz. The estimation of frequency response function revealed the dominant resonance frequencies between 0.60 Hz and 0.68 Hz. On the basis of our results illustrated here, the linear model of standing postural control was further concluded.

Effects of a Pelvic Belt on Hip Muscle Forces and Abdominal Muscle Activities During Isometric Hip Adduction and Abduction (등척성 엉덩관절 모음 및 벌림 시 골반 벨트가 엉덩관절 근육의 근력과 복부 근육 근 활성도에 미치는 영향)

  • Kang, Min-hyeok;Oh, Jae-seop
    • Physical Therapy Korea
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    • v.24 no.2
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    • pp.19-26
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    • 2017
  • Background: To improve lumbo-pelvic stability, passive support devices (i.e., a pelvic belt) are recommended clinically. Nevertheless, to understand the influence of passive support on lumbo-pelvic stability, it is necessary to examine the influence of a pelvic belt on the abdominal and hip abductor muscles. Objects: To examine the effects of a pelvic belt on the forces of the hip adductor and abductor muscles and activity of the abdominal muscles during isometric hip adduction and abduction. Methods: This study recruited 14 healthy men. All subjects performed isometric hip adduction and abduction with and without a pelvic belt in a neutral hip position. Load cells, wrapped with a non-elastic belt, were placed above the medial and lateral malleoli of the dominant leg to measure the muscle forces of the hip adductors and abductors, respectively. The forces of the hip adductors and abductors were measured using a load cell during isometric hip adduction and abduction, while the electromyographic activities of the bilateral rectus abdominis, internal oblique, and external oblique muscles were measured. Results: The forces generated by the hip adductors and abductors were significantly greater with the pelvic belt than without (p<.05). No significant differences in abdominal muscle activities between the two conditions were found (p>.05). Conclusion: These findings suggest that use of a pelvic belt could lead to effective strengthening exercise of hip muscles in individuals with sacroiliac joint pain.

Studies on Resistance to Organophosphorus Insecticide in the Brown Planthopper, Nilaparvata lugens $St{\aa}l$ (II) Difference of the Biochemical Characteristic (벼멸구의 유기인계(有機燐系) 살충제저항성(殺蟲劑抵抗性)에 대(對)하여(II) 생화학적특성(生化學的特性) 비교(比較))

  • Kim, Jeong-Wha;Hwang, Tae-Gu
    • Korean journal of applied entomology
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    • v.26 no.3 s.72
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    • pp.165-170
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    • 1987
  • This experiment was carried out to evaluate the difference in the biochemical characteristic of the brown planthoppers of the insecticide risistant, susceptible strains and their hybrid progenies. Activity of the esterase isozyme separated by electrophoresis method was remarkably high in the resistant strain as compared with the susceptible strain. Esterase activity between the insecticide-treated strains and the non-insecticide strains was not degraded in the resistant strain and the $F_1$, but remarkably degraded in the susceptible strain. The increase of esterase activity was associated with the development of resistance, and that was inherited with a dominant gene.

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Effect of SOx on HC-SCR Kinetics over Ag/Al2O3 Catalyst (SOx 함유 HC-SCR에서 산처리 Ag/Al2O3 촉매의 반응 특성)

  • Lee, Ju-Heon;Park, Jeong-Whan;Kim, Seong-Soo;Yoo, Seung-Joon;Kim, Jin-Gul
    • Journal of Hydrogen and New Energy
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    • v.22 no.5
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    • pp.714-720
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    • 2011
  • Ethanol was used as reducing agent to remove $NO_x$ exhaust from the stationary source. Pre-treatment with sulfuric acid over $Ag/Al_2O_3$ catalyst was dedicated to overcome the $SO_2$ poisoning effect. The $NO_x$ reduction experiment was performed under the simulated condition of power plant The increased surface area with higher CPSI devoted to increase de-$NO_x$ yield. De-$NO_x$ yield of the $NO_x$ exhaust containing 20 ppm of $SO_2$ increased after acid treatment with 0.7% $H_2SO_4$ over 4.0% $Ag/Al_2O_3$, where the increased dispersion of Ag found from the results of XRD and XPS was the dominant factor for the increased de-$NO_x$ yield. However, the reason for the decreased de-$NO_x$ yield with the acid treatment of higher concentration (1.0% and 2.0%) of $H_2SO_4$ was found to be due to the formation of $Ag_2SO_4$ crystallites found from XRD result. Acid-treated $Ag/Al_2O_3$ catalyst showed maximum de-$NO_x$ yield at higher temperature than non-treated $Ag/Al_2O_3$ catalyst did.

Bonding Properties of Steel-reinforced Polymer Cement Mortar Evaluated by Pull-off Test and FEM Modeling (폴리머 시멘트 모르타르의 철근부착력 평가를 위한 인발실험과 모델링)

  • Park, Dong-Cheon;Yoneda, Nobutosi;Cho, Bong-Suk
    • Journal of the Korea Institute of Building Construction
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    • v.14 no.3
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    • pp.216-222
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    • 2014
  • Chloride attack to reinforced concrete structures located in seaside can cause a serious problem of durability and maintenance during the service life. Corrosion of reinforced steel bars in concrete decreases the bond strength and finally causes the detachment of concrete cover. Polymer cement mortar is usually adopted to repair the deteriorated RC structures because of its strong bonding property. The recovered load-carrying capacity after the repair was simulated by non-linear FEM analysis. The properties of concrete, repairing materials, bonding materials and reinforced bar were used as input data. Four types of redispersible polymer powders were used as components of polymer cement mortar. Pull-off tests were carried out to examine the bond properties such as rigidity and strength. Effects of a corrosion inhibitor and the loss of reinforced bars due to the corrosion were also considered in this study. FEM modeling and analysis were conducted to propose the universal model. Physical bonding in the relationship between repair materials and steel reinforced bar is more dominant than chemical bonding.

Numerical Study of Gap Size Ratio Effect for Noncondensable Gas Ventilation in Condensers (응축기의 비응축 가스 배출 타입에 따른 틈 간격 비율의 영향에 대한 수치적 연구)

  • Je, Jun-Ho;Kim, Soo-Jea;Choi, Chi-Woong;Kim, Moo-Hwan
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.36 no.1
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    • pp.67-74
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    • 2012
  • A numerical analysis was carried out to estimate the effect of the gap size ratio on the performance of condensers under noncondensable gas ventilation using the porous medium approach (PMA). In the PMA, the details of the tube bundle in the condenser are considered to be those of a porous medium, and the flow resistance term is added in the momentum equation. Three-dimensional analysis of the condensation for a McAllister condenser was conducted with the PMA using Fluent and user-defined functions (UDFs). The gap size effect on the condensation was negligible under pure steam conditions. However, the gap size effect was dominant in condensation with noncondensable gas and external venting. As the gap size decreased, the condensation rate increased for noncondensable gas in an external venting system.

Use Impacts on Environmental Deteriorations of Trail and Campsite in Tokyusan National Park (덕유산 국립공원 등산로 및 야영장의. 환경훼손에 대한 이용영향)

  • 권태호;오구균;이준우
    • Korean Journal of Environment and Ecology
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    • v.7 no.2
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    • pp.241-251
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    • 1994
  • Use impacts on environmental deteriorations were studied on the four major trails and a campsite of Tokyuksan National Park in 1993. The entire width, bare width, maximum depth and slope of trail as the trail condition were significantly greater on the more heavily used trail. Percentages of rock-exposed, deepening, root-exposed points as the deterioration types of trail which were surveyed at the total of 106 points were higher and trail conditions were significantly different from those of non-deteriorated points. On the Paekryon trail, the damaged area more severe than Class 4 reaches about 910$m^2$/km and the use impact and deterioration on campsites were accelerated. The dominant trees of the the upper and lower layer in trail edge vegetation could be Quercus serrata and Acer pseudo-sieboldianum for Paekryon trail, Q. mongolica and Tripterigium regelii for the other trails. A. pseudo-sieboldianum, Q. serrata, Rhus trichocarpa, Fraxinus sieboldiana, Lespedeza maximowiczii, Symplocos chinensis for Pilosa were classified for tolerant species to use impacts.

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