• Archives of Pharmacal Research
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• v.23 no.2
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• pp.187-195
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• 2000

Nucleotide sequence extending 2,3-dihydroxybiphenyl 1,2-dioxygenase gene (pcbC) and 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase gene (pcbD) of Pseudomonas sp. DJ-12 was previously analyzed and the two genes were present in the order of pcbD-pcbC preceded by a promoter from Pseudomonas sp. DJ-12. In this study, a 3.8-kb nucleotide sequence located downstream of the pcbC gene was analyzed to have three open reading frames (ORFs) that are designated as orf1, pcbE and orf2 genes. All of the ORFs were preceded by each ribosome-binding sequence of 5-GGAXA-3 (X=G or A). However, no promoter-like sequence and transcription terminator sequence were found in the analyzed region, downstream of pcbC gene. Therefore, the gene cluster appeared to be present in the order of pcbD-pcbC-orf1-pcbE-orf2 as an operon, which is unique organization characterized so far in biphenyl- and PCB-degrading bacteria. The orf1 gene was composed of 1,224 base pairs which can encode a polypeptide of molecular weight 44,950 containing 405 amino acid residues. A deduced amino acid sequence of the orf1 gene product exhibited 21-33％ identity with those of indole dioxygenase and phenol hydroxylase components. The pcbE gene was composed of 783 base pairs encoding 2-hydroxypenta-2,4-dienoate hydratase involved in the 4-chlorobiphenyl catabolism. The orf2 gene was composed of 1,017 base pairs encoding a polypeptide of molecular weight 37,378 containing 338 amino acid residues. A deduced amino acid sequence of the orf2 gene product exhibited 31％ identity with that of a nitrilotriacetate monooxygenase component.

• Journal of Korean Institute of Industrial Engineers
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• v.29 no.1
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• pp.49-64
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• 2003

Although assembly sequence planning is an essential task in assembly process planning, it is known as one of the most difficult and time consuming jobs because its complexity is increased geometrically when the number of parts in an assembly is increased. The purpose of this study is to develop a more efficient algorithm for generating assembly sequences automatically. By considering subassemblies, a new heuristic method generates a preferred parallel assembly sequence that can be used in robotic assembly systems. A parallel assembly sequence concept provides a new representation scheme for an assembly in which the assembly sequence precedence information is not required. After an user inputs both the directional mating relation information and the mating condition information, an assembly product is divided into subgroups if the product has cut-vertices. Then, a virtual disassembly process is executed to generate alternate parallel assembly sequences with intermediate assembly stability. Through searching parts relations in the virtual disassembly process, stable subassemblies are extracted from translation-free parts along disassembling directions and this extraction continues until no more subassemblies are existed. Also, the arithmetic mean parallelism formula as a preference criterion is adapted to select the best parallel assembly sequence among others. Finally a preferred parallel assembly sequence is converted to an assembly BOM structure. The results from this study can be utilized for developing CAAPP(Computer-Aided Assembly Process Planning) systems as an efficient assembly sequence planning algorithm.

• The Transactions of the Korean Institute of Power Electronics
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• v.4 no.3
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• pp.275-284
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• 1999

Unbalanced source voltage in the 3-phase power system is decomposed into positive, negative and zero sequence c components. Also, assuming there is no neutral path in the system, the zero sequence component is not shown on the l load side. Therefore, in the unbalanced power system without neutral path. it is possible to provide balanced voltage to t the load side by compensating negative sequence component and also to regulate the voltage amplitude by controlling t the positive sequence component. In addition, the symmetrical components due to voltage unbalance can be effectively d detected on the synchronous reference frame by using dlongleftarrowq transformation. In this paper, an algorithm not only c compensating unbalanced source voltage by canceling the negative sequence component on the synchronous reference f frame but also maintaining load voltages constantly is proposed. Also a novel method for phase angle detection s synchronized by positive sequence component under unbalanced source voltage is suggested and this detected phase a angle is used for d-q transformation. The performances and characteristics of the proposed compensating system are a analyzed by simulation and verified through experimental results.

• BMB Reports
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• v.44 no.10
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• pp.653-658
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• 2011

Sclerotinia sclerotiorum fungus has three endoxylanases induced by wheat bran. In the first part, a partial xylanase sequence gene (90 bp) was isolated by PCR corresponding to catalytic domains (${\beta}5$ and ${\beta}6$ strands of this protein). The high homology of this sequence with xylanase of Botryotinia fuckeliana has permitted in the second part to amplify the XYN1 gene. Sequence analysis of DNA and cDNA revealed an ORF of 746 bp interrupted by a 65 bp intron, thus encoding a predicted protein of 226 amino acids. The mature enzyme (20.06 kDa), is coded by 188 amino acid (pI 9.26). XYN1 belongs to G/11 glycosyl hydrolases family with a conserved catalytic domain containing $E_{86}$ and $E_{178}$ residues. Bioinformatics analysis revealed that there was no Asn-X-Ser/Thr motif required for N-linked glycosylation in the deduced sequence however, five O-glycosylation sites could intervene in the different folding of xylanses isoforms and in their secretary pathway.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.31 no.9C
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• pp.889-896
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• 2006

The crest factor distribution of orthogonal frequency division multiplexing (OFDM) symbol sequences is evaluated and it is shown that OFDM symbol sequences with a short period are expected to have a high crest factor. The crest factor relationship between two input symbol sequences, Hamming distance D apart is also derived. Using these two results, we propose two criteria for a phase sequence set of the selected mapping (SLM) scheme and suggest the rows of the cyclic Hadamard matrix constructed from an m-sequence as the near optimal phase sequence set of the SLM scheme.

• Biomedical Science Letters
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• v.8 no.3
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• pp.189-193
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• 2002

We have identified and analyzed the 5'-coding region of SCN5A gene in Korean genome. Although its sequence has already been reported in western countries it is still important to confirm our own sequence for the establishment of Korean-suitable diagnosis on genetic basis. Total RNAs were obtained from three healthy Korean adult hearts and reversely transcribed. RT products were then subjected to PCR reaction followed by DNA sequencing. Three different sets of SCN5A primers were designed and used for the amplification of 5'-coding region of SCN5A from Korean genome. Amplified sequence was roughly one-10th of the entire SCN5A mRNA in size and its detailed sequence was completely matched up to the previously reported sequence. There was no difference between three heart samples, either. So, SCN5A was concluded as the relatively stable gene comparing to other genes that are involved in long QT syndrome.

• Journal of the Society of Naval Architects of Korea
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• v.54 no.5
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• pp.368-377
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• 2017

Outfittings of offshore plants and high value-added vessels, such as FPSO, drillship, are much more than outfittings of general ships. So the complexity of the outfittings is increased and the importance of outfittings has also increased. But, there is no clear method to define the installation sequence of outfittings, yet. In this study, we developed the algorithm to define the installation sequence of outfittings by applying variables and constraints related to outfitting, such as process parameters, weighting coefficients, installation constraints. Also, we developed the application that applied the algorithm and compared cases by changing the weighting coefficients of process parameters. We verified the practicality of the algorithm by developing the application. The results of this study are that the accuracy of the outfitting planning is improved and efficient lead time can be predicted by defining the installation sequence of outfittings.

• Proceedings of the KOSOMBE Conference
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• v.1989 no.05
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• pp.35-37
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• 1989

A new MR angiography technique using a composite sequence for the suppression of static sample signals is proposed and verified with experiments. When the composite [$90^{\circ}-{\tau}-180^{\circ}-2{\tau}-180^{\circ}-{\tau}$] sequence is applied, the large signal from the static sample is sufficiently suppressed but the signal from fresh inflow sample of which amplitude. is observed without suppression. These properties are appropriate for angiographic applications. In this paper, a modified line scan method (Block line scan angiography) incorporated with the composite [$90^{\circ}-{\tau}-180^{\circ}-2{\tau}-180^{\circ}-{\tau}$] sequence is used to obtain flow-only images, i.e., angiograms. The block line scan method improves the resolution in the flow-direction at the expense of imaging time. With the composite sequence, there is no need for subtraction procedure as in the most conventional angiographic methods. Experimental results for a phantom and a normal volunteer with KAIS 2.0 Tesla MRI system are shown.

• Journal of Microbiology and Biotechnology
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• v.5 no.3
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• pp.125-131
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• 1995

A 3, 346 bp of the cdd upstream region in Bacillus subtilis was sequenced from the pSO1 (Song BH and J Neuhard. 1989. Mol. Gen. Genet 216: 462-468) and sequence homology was searched to the known genes in Genbank and European Molecular Biology Laboratory databanks. Five complete and one truncated putative coding sequences deduced from the nucleotide sequence were found through the ORF searching by Genetyx and Macvector software, and one of them was identified as the dgk (diacylglycerol kinase) gene and another, a truncated one, as the phoH (phosphate starvation-inducible gene) gene. The B. subtilis dgk gene, having a role for response to several environmental stress signals, revealed an open reading frame of 134 amino acids with 43.1% of sequence identity to the Streptococcus mutans dgk gene. The carboxy terminal 59 residues of the truncated phoH gene showed 52.7% and 34.5% of sequence identity in amino acids with the corresponding genes of Mycobacterium leprae and Escherichia coli. The four remaining coding sequences consisting of 115, 421, 91, and 91 residues were thought to be unknown ORFs because they have no significant similarity to known genes.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.34 no.4
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• pp.82-88
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• 2011

A typical job sequencing problem is studied in this research to improve productivities in manufacturing companies. The problem consists of two-stage parts and assembly processes. Two parts are provided independently each other and then two sequential assembly processes are followed. A new heuristic is developed to solve the new type of sequencing problem. Initial solution is developed in the first stage and then the initial solution is improved in the second stage. In the first stage, a longer part manufacturing time for each job is selected between two, and then a sequence is determined by descending order of the times. This initial sequence is compared with Johnson's sequence obtained from 2-machine assembly times. Any mismatches are tried to switch as one possible alternative and completion time is calculated to determine whether to accept the new sequence or not to replace the current sequence. Searching process stops if no more improvement can be made.